PT	AU	BA	BE	GP	AF	BF	CA	TI	SO	SE	BS	LA	DT	CT	CY	CL	SP	HO	DE	ID	AB	C1	RP	EM	RI	OI	FU	FX	CR	NR	TC	Z9	U1	U2	PU	PI	PA	SN	EI	BN	J9	JI	PD	PY	VL	IS	PN	SU	SI	MA	BP	EP	AR	DI	D2	EA	PG	WC	SC	GA	UT	PM	OA	HC	HP	DA
J	Phan, T				Tung Phan			Novel coronavirus: From discovery to clinical diagnostics	INFECTION GENETICS AND EVOLUTION			English	Article						China; coronavirus; Bat; Pneumonia		A novel coronavirus designated as 2019-nCoV first appeared in Wuhan, China in late December 2019. Dozens of people died in China, and thousands of people infected as 2019-nCoV continues to spread around the world. We have described the discovery, emergence, genomic characteristics, and clinical diagnostics of 2019-nCoV.	[Tung Phan] Univ Pittsburgh, Div Clin Microbiol, Pittsburgh, PA 15260 USA; [Tung Phan] Univ Pittsburgh, Med Ctr, Pittsburgh, PA 15260 USA	Phan, T (reprint author), Univ Pittsburgh, Div Clin Microbiol, Pittsburgh, PA 15260 USA.; Phan, T (reprint author), Univ Pittsburgh, Med Ctr, Pittsburgh, PA 15260 USA.	phantg@upmc.edu			Division of Clinical Microbiology, University of Pittsburgh Medical Center	We acknowledge support from Division of Clinical Microbiology, University of Pittsburgh Medical Center.	Babady NE, 2018, J CLIN MICROBIOL, V56, DOI 10.1128/JCM.01658-17; Beckmann C, 2016, J MED VIROL, V88, P1319, DOI 10.1002/jmv.24492; Fung TS, 2019, ANNU REV MICROBIOL, V73, P529, DOI 10.1146/annurev-micro-020518-115759; Huang HS, 2018, CLIN MICROBIOL INFEC, V24, P1055, DOI 10.1016/j.cmi.2017.11.018; Luk HKH, 2019, INFECT GENET EVOL, V71, P21, DOI 10.1016/j.meegid.2019.03.001; Ramadan Nour, 2019, Germs, V9, P35, DOI 10.18683/germs.2019.1155; Wong ACP, 2019, VIRUSES-BASEL, V11, DOI 10.3390/v11020174; Zhu N, 2020, NEW ENGL J MED, V382, P727, DOI 10.1056/NEJMoa2001017	8	0	0	515	515	ELSEVIER	AMSTERDAM	RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS	1567-1348	1567-7257		INFECT GENET EVOL	Infect. Genet. Evol.	APR	2020	79								104211	10.1016/j.meegid.2020.104211			2	Infectious Diseases	Infectious Diseases	KH7YP	WOS:000510866400017	32007627	Bronze			2020-04-01	
J	Paraskevis, D; Kostaki, EG; Magiorkinis, G; Panayiotakopoulos, G; Sourvinos, G; Tsiodras, S				Paraskevis, D.; Kostaki, E. G.; Magiorkinis, G.; Panayiotakopoulos, G.; Sourvinos, G.; Tsiodras, S.			Full-genome evolutionary analysis of the novel corona virus (2019-nCoV) rejects the hypothesis of emergence as a result of a recent recombination event	INFECTION GENETICS AND EVOLUTION			English	Article						Novel coronavirus; Genomic sequence analysis; Phylogenetic analysis; Recombination; Origin; Molecular epidemiology	RESPIRATORY SYNDROME CORONAVIRUS; SARS-CORONAVIRUS; COV	Background: A novel coronavirus (2019-nCoV) associated with human to human transmission and severe human infection has been recently reported from the city of Wuhan in China. Our objectives were to characterize the genetic relationships of the 2019-nCoV and to search for putative recombination within the subgenus of sarbecovirus. Methods: Putative recombination was investigated by RDP4 and Simplot v3.5.1 and discordant phylogenetic clustering in individual genomic fragments was confirmed by phylogenetic analysis using maximum likelihood and Bayesian methods. Results: Our analysis suggests that the 2019-nCoV although closely related to BatCoV RaTG13 sequence throughout the genome (sequence similarity 96.3%), shows discordant clustering with the Bat_SARS-like coronavirus sequences. Specifically, in the 5'-part spanning the first 11,498 nucleotides and the last 3'-part spanning 24,341-30,696 positions, 2019-nCoV and RaTG13 formed a single cluster with Bat_SARS-like coronavirus sequences, whereas in the middle region spanning the 3'-end of ORF1a, the ORF1b and almost half of the spike regions, 2019-nCoV and RaTG13 grouped in a separate distant lineage within the sarbecovirus branch. Conclusions: The levels of genetic similarity between the 2019-nCoV and RaTG13 suggest that the latter does not provide the exact variant that caused the outbreak in humans, but the hypothesis that 2019-nCoV has originated from bats is very likely. We show evidence that the novel coronavirus (2019-nCov) is not-mosaic consisting in almost half of its genome of a distinct lineage within the betacoronavirus. These genomic features and their potential association with virus characteristics and virulence in humans need further attention.	[Paraskevis, D.; Kostaki, E. G.; Magiorkinis, G.] Natl & Kapodistrian Univ Athens, Med Sch, Dept Hyg Epidemiol & Med Stat, Athens, Greece; [Panayiotakopoulos, G.] NPHO, Athens, Greece; [Sourvinos, G.] Univ Crete, Sch Med, Lab Clin Virol, Iraklion, Greece; [Tsiodras, S.] Natl & Kapodistrian Univ Athens, Med Sch, Athens, Greece	Paraskevis, D (reprint author), Univ Athens, Sch Med, Dept Hyg Epidemiol & Med Stat, 75 Mikras Asias St, Athens 11527, Greece.	dparask@med.uoa.gr	Ribeiro, Nuno/AAH-2299-2020	Paraskevis, Dimitrios/0000-0001-6167-7152; Kostaki, Evangelia/0000-0002-3346-0930			Al Hajjar S, 2013, ANN SAUDI MED, V33, P427, DOI 10.5144/0256-4947.2013.427; Alagaili AN, 2014, MBIO, V5, DOI 10.1128/mBio.00884-14; Babcock GJ, 2004, J VIROL, V78, P4552, DOI 10.1128/JVI.78.9.4552-4560.2004; Cui J, 2019, NAT REV MICROBIOL, V17, P181, DOI 10.1038/s41579-018-0118-9; Drosten C, 2003, NEW ENGL J MED, V348, P1967, DOI 10.1056/NEJMoa030747; ECDC, 2020, ECDC TECHN REP; Fehr AR, 2015, METHODS MOL BIOL, V1282, P1, DOI 10.1007/978-1-4939-2438-7_1; Guan Y, 2003, SCIENCE, V302, P276, DOI 10.1126/science.1087139; Huelsenbeck JP, 2001, BIOINFORMATICS, V17, P754, DOI 10.1093/bioinformatics/17.8.754; Hui DS, 2020, INT J INFECT DIS, V91, P264, DOI 10.1016/j.ijid.2020.01.009; Ithete NL, 2013, EMERG INFECT DIS, V19, P1697, DOI 10.3201/eid1910.130946; Ji W, 2020, INT J INFECT DIS, V91, P264; Kahn Jeffrey S, 2005, Pediatr Infect Dis J, V24, pS223, DOI 10.1097/01.inf.0000188166.17324.60; Katoh K, 2013, MOL BIOL EVOL, V30, P772, DOI 10.1093/molbev/mst010; Ksiazek TG, 2003, NEW ENGL J MED, V348, P1953, DOI 10.1056/NEJMoa030781; Lau SKP, 2005, P NATL ACAD SCI USA, V102, P14040, DOI 10.1073/pnas.0506735102; Li WD, 2005, SCIENCE, V310, P676, DOI 10.1126/science.1118391; Li WH, 2005, EMBO J, V24, P1634, DOI 10.1038/sj.emboj.7600640; Lole KS, 1999, J VIROL, V73, P152, DOI 10.1128/JVI.73.1.152-160.1999; Magiorkinis G, 2004, J MED VIROL, V74, P369, DOI 10.1002/jmv.20187; Martin DP, 2015, VIRUS EVOL, V1, DOI 10.1093/ve/vev003; Paules CI, 2020, JAMA-J AM MED ASSOC, V323, P707, DOI 10.1001/jama.2020.0757; Peiris JSM, 2003, LANCET, V361, P1319, DOI 10.1016/S0140-6736(03)13077-2; Perlman S, 2020, NEW ENGL J MED, V382, P760, DOI 10.1056/NEJMe2001126; Stecher Glen, 2020, Mol Biol Evol, V37, P1237, DOI 10.1093/molbev/msz312; Swofford D.L., 2003, PAUP PHYLOGENETIC AN; WHO, 2018, ANN REV DIS PRIOR RE; World Health Organization, 2020, NOV COR 2019 NCOV SI; Zaki AM, 2012, NEW ENGL J MED, V367, P1814, DOI 10.1056/NEJMoa1211721; Zhou P, 2020, DISCOVERY NOVEL CORO; Zhu N, 2020, N ENGL J MED	31	4	4	473	473	ELSEVIER	AMSTERDAM	RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS	1567-1348	1567-7257		INFECT GENET EVOL	Infect. Genet. Evol.	APR	2020	79								104212	10.1016/j.meegid.2020.104212			4	Infectious Diseases	Infectious Diseases	KH7YP	WOS:000510866400018	32004758	Bronze			2020-04-01	
J	Huang, CL; Wang, YM; Li, XW; Ren, LL; Zhao, JP; Hu, Y; Zhang, L; Fan, GH; Xu, JY; Gu, XY; Cheng, ZS; Yu, T; Xia, JA; Wei, Y; Wu, WJ; Xie, XL; Yin, W; Li, H; Liu, M; Xiao, Y; Gao, H; Guo, L; Xie, JG; Wang, GF; Jiang, RM; Gao, ZC; Jin, Q; Wang, JW; Cao, B				Huang, Chaolin; Wang, Yeming; Li, Xingwang; Ren, Lili; Zhao, Jianping; Hu, Yi; Zhang, Li; Fan, Guohui; Xu, Jiuyang; Gu, Xiaoying; Cheng, Zhenshun; Yu, Ting; Xia, Jiaan; Wei, Yuan; Wu, Wenjuan; Xie, Xuelei; Yin, Wen; Li, Hui; Liu, Min; Xiao, Yan; Gao, Hong; Guo, Li; Xie, Jungang; Wang, Guangfa; Jiang, Rongmeng; Gao, Zhancheng; Jin, Qi; Wang, Jianwei; Cao, Bin			Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China	LANCET			English	Article							EAST RESPIRATORY SYNDROME; INFLAMMATORY CYTOKINES; SARS	Background A recent cluster of pneumonia cases in Wuhan, China, was caused by a novel betacoronavirus, the 2019 novel coronavirus (2019-nCoV). We report the epidemiological, clinical, laboratory, and radiological characteristics and treatment and clinical outcomes of these patients. Methods All patients with suspected 2019-nCoV were admitted to a designated hospital in Wuhan. We prospectively collected and analysed data on patients with laboratory-confirmed 2019-nCoV infection by real-time RT-PCR and next-generation sequencing. Data were obtained with standardised data collection forms shared by WHO and the International Severe Acute Respiratory and Emerging Infection Consortium from electronic medical records. Researchers also directly communicated with patients or their families to ascertain epidemiological and symptom data. Outcomes were also compared between patients who had been admitted to the intensive care unit (ICU) and those who had not. Findings By Jan 2, 2020, 41 admitted hospital patients had been identified as having laboratory-confirmed 2019-nCoV infection. Most of the infected patients were men (30 [73%] of 41); less than half had underlying diseases (13 [32%]), including diabetes (eight [20%]), hypertension (six [15%]), and cardiovascular disease (six [15%]). Median age was 49.0 years (IQR 41.0-58.0). 27 (66%) of 41 patients had been exposed to Huanan seafood market. One family cluster was found. Common symptoms at onset of illness were fever (40 [98%] of 41 patients), cough (31 [76%]), and myalgia or fatigue (18 [44%]); less common symptoms were sputum production (11 [28%] of 39), headache (three [8%] of 38), haemoptysis (two [5%] of 39), and diarrhoea (one [3%] of 38). Dyspnoea developed in 22 (55%) of 40 patients (median time from illness onset to dyspnoea 8.0 days [IQR 5.0-13.0]). 26 (63%) of 41 patients had lymphopenia. All 41 patients had pneumonia with abnormal findings on chest CT. Complications included acute respiratory distress syndrome (12 [29%]), RNAaemia (six [15%]), acute cardiac injury (five [12%]) and secondary infection (four [10%]). 13 (32%) patients were admitted to an ICU and six (15%) died. Compared with non-ICU patients, ICU patients had higher plasma levels of IL2, IL7, IL10, GSCF, IP10, MCP1, MIP1A, and TNF alpha. Interpretation The 2019-nCoV infection caused clusters of severe respiratory illness similar to severe acute respiratory syndrome coronavirus and was associated with ICU admission and high mortality. Major gaps in our knowledge of the origin, epidemiology, duration of human transmission, and clinical spectrum of disease need fulfilment by future studies. Copyright (C) 2020 Elsevier Ltd. All rights reserved.	[Huang, Chaolin; Zhang, Li; Yu, Ting; Xia, Jiaan; Wei, Yuan; Wu, Wenjuan; Xie, Xuelei] Jin Yin tan Hosp, Wuhan, Peoples R China; [Wang, Yeming; Fan, Guohui; Gu, Xiaoying; Li, Hui; Cao, Bin] China Japan Friendship Hosp, Ctr Resp Med, Natl Clin Res Ctr Resp Dis, Dept Pulm & Crit Care Med, Beijing, Peoples R China; [Fan, Guohui; Gu, Xiaoying] China Japan Friendship Hosp, Inst Clin Med Sci, Beijing, Peoples R China; [Liu, Min] China Japan Friendship Hosp, Dept Radiol, Beijing, Peoples R China; [Wang, Yeming; Fan, Guohui; Gu, Xiaoying; Li, Hui; Cao, Bin] Chinese Acad Med Sci, Inst Resp Med, Peking Union Med Coll, Beijing, Peoples R China; [Wang, Yeming; Li, Hui; Cao, Bin] Capital Med Univ, Dept Resp Med, Beijing, Peoples R China; [Li, Xingwang; Jiang, Rongmeng] Capital Med Univ, Beijing Ditan Hosp, Clin & Res Ctr Infect Dis, Beijing, Peoples R China; [Ren, Lili; Xiao, Yan; Guo, Li; Jin, Qi; Wang, Jianwei] Chinese Acad Med Sci & Peking Union Med Coll, NHC Key Lab Syst Biol Pathogens & Christophe Meri, Inst Pathogen Biol, Beijing 100730, Peoples R China; [Gao, Hong] Chinese Acad Med Sci & Peking Union Med Coll, Inst Lab Anim Sci, Beijing, Peoples R China; [Zhao, Jianping; Xie, Jungang] Huazhong Univ Sci & Technol, Tongji Med Coll, Tongji Hosp, Wuhan, Peoples R China; [Hu, Yi; Yin, Wen] Huazhong Univ Sci & Technol, Tongji Med Coll, Dept Pulm & Crit Care Med, Cent Hosp Wuhan, Wuhan, Peoples R China; [Xu, Jiuyang] Tsinghua Univ, Sch Med, Beijing, Peoples R China; [Cheng, Zhenshun] Wuhan Univ, Resp Med, Zhongnan Hosp, Wuhan, Peoples R China; [Wang, Guangfa] Peking Univ First Hosp, Dept Pulm & Crit Care Med, Beijing, Peoples R China; [Gao, Zhancheng] Peking Univ Peoples Hosp, Dept Pulm & Crit Care Med, Beijing, Peoples R China; [Cao, Bin] Tsinghua Univ Peking Univ Joint Ctr Life Sci, Beijing, Peoples R China	Wang, JW (reprint author), Chinese Acad Med Sci & Peking Union Med Coll, NHC Key Lab Syst Biol Pathogens & Christophe Meri, Inst Pathogen Biol, Beijing 100730, Peoples R China.; Cao, B (reprint author), China Japan Friendship Hosp, Dept Pulm & Crit Care Med, Beijing 100029, Peoples R China.	wangjw28@163.com; caobin_ben@163.com	Ribeiro, Nuno/AAH-2299-2020		Ministry of Science and Technology [2020YFC0841300]; Chinese Academy of Medical Sciences (CAMS) Innovation Fund for Medical Sciences [CIFMS 2018-I2M-1-003]; National Science Grant for Distinguished Young Scholars [81425001/H0104]; National Key Research and Development Program of China [2018YFC1200102]; Beijing Science and Technology Project [Z19110700660000]; CAMS Innovation Fund for Medical Sciences [2016-I2M-1-014]; National Mega-projects for Infectious Diseases in China [2017ZX10103004, 2018ZX10305409]	This work is funded by the Special Project for Emergency of the Ministry of Science and Technology (2020YFC0841300) Chinese Academy of Medical Sciences (CAMS) Innovation Fund for Medical Sciences (CIFMS 2018-I2M-1-003), a National Science Grant for Distinguished Young Scholars (81425001/H0104), the National Key Research and Development Program of China (2018YFC1200102), The Beijing Science and Technology Project (Z19110700660000), CAMS Innovation Fund for Medical Sciences (2016-I2M-1-014), and National Mega-projects for Infectious Diseases in China (2017ZX10103004 and 2018ZX10305409). We acknowledge all health-care workers involved in the diagnosis and treatment of patients in Wuhan; we thank the Chinese National Health Commission for coordinating data collection for patients with 2019-nCoV infection; we thank WHO and the International Severe Acute Respiratory and Emerging Infections Consortium (ISARIC) for sharing data collection templates publicly on the website; and we thank Prof Chen Wang and Prof George F Gao for guidance in study design and interpretation of results.	Arabi YM, 2018, AM J RESP CRIT CARE, V197, P757, DOI 10.1164/rccm.201706-1172OC; Arabi YM, 2018, TRIALS, V19, DOI 10.1186/s13063-017-2427-0; Assiri A, 2013, LANCET INFECT DIS, V13, P752, DOI 10.1016/S1473-3099(13)70204-4; CDC, 2020, 1 TRAV REL CAS 2019; Chu CM, 2004, THORAX, V59, P252, DOI 10.1136/thorax.2003.012658; Cui J, 2019, NAT REV MICROBIOL, V17, P181, DOI 10.1038/s41579-018-0118-9; de Groot RJ, 2013, J VIROL, V87, P7790, DOI 10.1128/JVI.01244-13; Drosten C, 2003, NEW ENGL J MED, V348, P1967, DOI 10.1056/NEJMoa030747; Falzarano D, 2013, NAT MED, V19, P1313, DOI 10.1038/nm.3362; Faure E, 2014, PLOS ONE, V9, DOI 10.1371/journal.pone.0088716; Gao Chang, 2020, Crit Care Med, V48, P451, DOI 10.1097/CCM.0000000000004207; GARNER JS, 1988, AM J INFECT CONTROL, V16, P128, DOI 10.1016/0196-6553(88)90053-3; Ge XY, 2013, NATURE, V503, P535, DOI 10.1038/nature12711; He L, 2006, J PATHOL, V210, P288, DOI 10.1002/path.2067; Kidney disease: improving global outcomes (KDIGO) acute kidney injury work group, 2012, KDIGO CLIN PRACTICE; Ksiazek TG, 2003, NEW ENGL J MED, V348, P1953, DOI 10.1056/NEJMoa030781; Kuiken T, 2003, LANCET, V362, P263, DOI 10.1016/S0140-6736(03)13967-0; Lansbury L, 2019, COCHRANE DB SYST REV, DOI 10.1002/14651858.CD010406.pub3; Lee N, 2003, NEW ENGL J MED, V348, P1986, DOI 10.1056/NEJMoa030685; Mahallawi WH, 2018, CYTOKINE, V104, P8, DOI 10.1016/j.cyto.2018.01.025; Perlman S, 2009, NAT REV MICROBIOL, V7, P439, DOI 10.1038/nrmicro2147; Richman DD, 2016, CLIN VIROLOGY; Sanz F, 2011, EUR RESP J S55, V38, P2492; Sheahan Timothy P, 2020, Nat Commun, V11, P222, DOI 10.1038/s41467-019-13940-6; Sheahan TP, 2017, SCI TRANSL MED, V9, DOI 10.1126/scitranslmed.aal3653; Stockman LJ, 2006, PLOS MED, V3, P1525, DOI 10.1371/journal.pmed.0030343; Tan W, 2019, NOVEL CORONAVIRUS GE; Wang Manli, 2013, Virologica Sinica, V28, P315, DOI 10.1007/s12250-013-3402-x; *WHO, 2003, SUMM PROB SARS CAS O; WHO, 2020, NOVEL CORONAVIR 0114; WHO, 2020, NOVEL CORONAVIR 0112; WHO, 2020, NOVEL CORONAVIR 0117; WHO, 2020, NOVEL CORONAVIR 0121; Wong CK, 2004, CLIN EXP IMMUNOL, V136, P95, DOI 10.1111/j.1365-2249.2004.02415.x; World Health Organization, 2019, MIDDL E RESP SYNDR C; World Health Organization, 2020, CLIN MAN SEV AC RESP; Zaki AM, 2012, NEW ENGL J MED, V367, P1814, DOI 10.1056/NEJMoa1211721	37	124	123	465	465	ELSEVIER SCIENCE INC	NEW YORK	STE 800, 230 PARK AVE, NEW YORK, NY 10169 USA	0140-6736	1474-547X		LANCET	Lancet	FEB 15	2020	395	10223					497	506		10.1016/S0140-6736(20)30183-5			10	Medicine, General & Internal	General & Internal Medicine	KN1CG	WOS:000514576900032	31986264	Bronze			2020-04-01	
J	Ji, W; Wang, W; Zhao, XF; Zai, JJ; Li, XG				Ji, Wei; Wang, Wei; Zhao, Xiaofang; Zai, Junjie; Li, Xingguang			Cross-species transmission of the newly identified coronavirus 2019-nCoV	JOURNAL OF MEDICAL VIROLOGY			English	Article						2019-nCoV; codon usage bias; cross-species transmission; phylogenetic analysis; recombination	NATURAL-POPULATIONS; RECOMBINATION; VIRUS; FEVER; SARS	The current outbreak of viral pneumonia in the city of Wuhan, China, was caused by a novel coronavirus designated 2019-nCoV by the World Health Organization, as determined by sequencing the viral RNA genome. Many initial patients were exposed to wildlife animals at the Huanan seafood wholesale market, where poultry, snake, bats, and other farm animals were also sold. To investigate possible virus reservoir, we have carried out comprehensive sequence analysis and comparison in conjunction with relative synonymous codon usage (RSCU) bias among different animal species based on the 2019-nCoV sequence. Results obtained from our analyses suggest that the 2019-nCoV may appear to be a recombinant virus between the bat coronavirus and an origin-unknown coronavirus. The recombination may occurred within the viral spike glycoprotein, which recognizes a cell surface receptor. Additionally, our findings suggest that 2019-nCoV has most similar genetic information with bat coronovirus and most similar codon usage bias with snake. Taken together, our results suggest that homologous recombination may occur and contribute to the 2019-nCoV cross-species transmission.	[Ji, Wei] Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Microbiol, Beijing, Peoples R China; [Wang, Wei] Guangxi Univ Chinese Med, Affiliated Hosp 1, Dept Spleen & Stomach Dis, Nanning, Peoples R China; [Zhao, Xiaofang] Guangxi Univ Chinese Med, Ruikang Hosp, Dept Sci & Technol, Nanning, Peoples R China; [Zai, Junjie] Ningbo Univ, Sch Med, Immunol Innovat Team, Ningbo, Peoples R China; [Li, Xingguang] Wuhan Univ Bioengn, Hubei Engn Res Ctr Viral Vector, Wuhan, Peoples R China	Ji, W (reprint author), Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Microbiol, Beijing, Peoples R China.	j102938@126.com	zorlu, taha/AAH-6820-2020		Natural Science Foundation of Guangxi Province of ChinaNational Natural Science Foundation of Guangxi Province [2017GXNSFAA198080]; Project of Guangxi Health Committee [Z20191111]; K.C. Wong Magna Fund		Bahir I, 2009, MOL SYST BIOL, V5, DOI 10.1038/msb.2009.71; Bollyky PL, 1996, J MOL EVOL, V42, P97, DOI 10.1007/BF02198834; Chan PKS, 2002, CLIN INFECT DIS, V34, pS58, DOI 10.1086/338820; CLAVEL F, 1989, J VIROL, V63, P1455, DOI 10.1128/JVI.63.3.1455-1459.1989; Colina R, 2004, J GEN VIROL, V85, P31, DOI 10.1099/vir.0.19472-0; Cunha CB, 2014, VIRULENCE, V5, P650, DOI 10.4161/viru.32077; Graham RL, 2010, J VIROL, V84, P3134, DOI 10.1128/JVI.01394-09; Guan Y, 2003, SCIENCE, V302, P276, DOI 10.1126/science.1087139; Hall T.A., 1999, NUCL ACIDS S SER, V41, P95, DOI DOI 10.1021/BK-1999-0734.CH008; Howe H, 2019, BIOMEDICAL INFORM CA, P267; Howley DMKP, 2013, FIELDS VIROLOGY, V1, p830P; Ji W, 2014, INFECT GENET EVOL, V25, P69, DOI 10.1016/j.meegid.2014.04.008; Katoh K, 2013, MOL BIOL EVOL, V30, P772, DOI 10.1093/molbev/mst010; Liu XS, 2012, VIROL J, V9, DOI 10.1186/1743-422X-9-68; Lole KS, 1999, J VIROL, V73, P152, DOI 10.1128/JVI.73.1.152-160.1999; Lu HZ, 2020, J MED VIROL, V92, P401, DOI 10.1002/jmv.25678; MacLachlan NJ, 2017, FENNERS VET VIROLOGY, P393; Smith RD, 2006, SOC SCI MED, V63, P3113, DOI 10.1016/j.socscimed.2006.08.004; Stamatakis A, 2014, BIOINFORMATICS, V30, P1312, DOI 10.1093/bioinformatics/btu033; Tolou HJG, 2001, J GEN VIROL, V82, P1283, DOI 10.1099/0022-1317-82-6-1283; Valencia A, 2016, PLOS ONE, V11, DOI 10.1371/journal.pone.0151001; Wikipedia, 2020, MAN BAND KRAIT; Wikipedia, 2020, CHIN COBR; World Health Organisation, 2003, CONS DOC EP SEV AC R; World Health Organization, 2019, COR; WRIGHT F, 1990, GENE, V87, P23, DOI 10.1016/0378-1119(90)90491-9; Yu XJ, 2011, NEW ENGL J MED, V364, P1523, DOI 10.1056/NEJMoa1010095; Zhang NR, 2020, J MED VIROL, V92, P408, DOI 10.1002/jmv.25674	28	14	14	417	417	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0146-6615	1096-9071		J MED VIROL	J. Med. Virol.	APR	2020	92	4			SI		433	440		10.1002/jmv.25682			8	Virology	Virology	KM6QU	WOS:000514263400007	31967321	Bronze			2020-04-01	
J	Zhu, N; Zhang, DY; Wang, WL; Li, XW; Yang, B; Song, JD; Zhao, X; Huang, BY; Shi, WF; Lu, RJ; Niu, PH; Zhan, FX; Ma, XJ; Wang, DY; Xu, WB; Wu, GZ; Gao, GGF; Tan, WJ				Zhu, Na; Zhang, Dingyu; Wang, Wenling; Li, Xingwang; Yang, Bo; Song, Jingdong; Zhao, Xiang; Huang, Baoying; Shi, Weifeng; Lu, Roujian; Niu, Peihua; Zhan, Faxian; Ma, Xuejun; Wang, Dayan; Xu, Wenbo; Wu, Guizhen; Gao, George F.; Tan, Wenjie		China Novel Coronavirus	A Novel Coronavirus from Patients with Pneumonia in China, 2019	NEW ENGLAND JOURNAL OF MEDICINE			English	Article							RESPIRATORY SYNDROME; EPIDEMIOLOGY; SARS	In December 2019, a cluster of patients with pneumonia of unknown cause was linked to a seafood wholesale market in Wuhan, China. A previously unknown betacoronavirus was discovered through the use of unbiased sequencing in samples from patients with pneumonia. Human airway epithelial cells were used to isolate a novel coronavirus, named 2019-nCoV, which formed a clade within the subgenus sarbecovirus, Orthocoronavirinae subfamily. Different from both MERS-CoV and SARS-CoV, 2019-nCoV is the seventh member of the family of coronaviruses that infect humans. Enhanced surveillance and further investigation are ongoing. (Funded by the National Key Research and Development Program of China and the National Major Project for Control and Prevention of Infectious Disease in China.) The authors report the emergence and isolation of a previously unknown betacoronavirus, the seventh member of the family of coronaviruses that infect humans, in Wuhan, China. Findings in three patients are described.	[Zhu, Na; Wang, Wenling; Zhao, Xiang; Huang, Baoying; Lu, Roujian; Niu, Peihua; Ma, Xuejun; Wang, Dayan; Xu, Wenbo; Wu, Guizhen; Gao, George F.; Tan, Wenjie] Chinese Ctr Dis Control & Prevent, Natl Inst Viral Dis Control & Prevent, NHC Key Lab Biosafety, Beijing, Peoples R China; [Li, Xingwang] Capital Med Univ, Beijing Ditan Hosp, Dept Infect Dis, Beijing, Peoples R China; [Zhang, Dingyu] Chinese Acad Sci, Wuhan Jinyintan Hosp, Wuhan, Hubei, Peoples R China; [Yang, Bo; Zhan, Faxian] Chinese Acad Sci, Hubei Prov Ctr Dis Control & Prevent, Div Viral Dis Detect, Wuhan, Hubei, Peoples R China; [Tan, Wenjie] Chinese Acad Sci, Ctr Biosafety Mega Sci, Wuhan, Hubei, Peoples R China; [Shi, Weifeng] Shandong First Med Univ, Jinan, Shandong, Peoples R China; [Shi, Weifeng] Shandong Acad Med Sci, Jinan, Shandong, Peoples R China	Wu, GZ; Tan, WJ (reprint author), China CDC, Natl Inst Viral Dis Control & Prevent, NHC Key Lab Biosafety, 155 Changbai Rd, Beijing 102206, Peoples R China.; Gao, GGF (reprint author), China CDC, Natl Inst Viral Dis Control & Prevent, Beijing 102206, Peoples R China.	wugz@ivdc.chinacdc.cn; gaof@im.ac.cn; tanwj@ivdc.chinacdc.cn			National Key Research and Development Program of China [2016YFD0500301]; National Major Project for Control and Prevention of Infectious Disease in China [2018ZX10101002]	This work was supported by grants from the National Key Research and Development Program of China (2016YFD0500301) and the National Major Project for Control and Prevention of Infectious Disease in China (2018ZX10101002).	[Anonymous], 2019, REP CLUST PNEUM UNKN; [Anonymous], 2020, REP NOV COR INF PNEU; Armstrong GL, 2019, NEW ENGL J MED, V381, P2569, DOI 10.1056/NEJMsr1813907; Cui J, 2019, NAT REV MICROBIOL, V17, P181, DOI 10.1038/s41579-018-0118-9; Drosten C, 2003, NEW ENGL J MED, V348, P1967, DOI 10.1056/NEJMoa030747; Gao GF, 2018, CELL, V172, P1157, DOI 10.1016/j.cell.2018.02.025; Jonsdottir HR, 2016, VIROL J, V13, DOI 10.1186/s12985-016-0479-5; Ksiazek TG, 2003, NEW ENGL J MED, V348, P1953, DOI 10.1056/NEJMoa030781; Liu GS, 2019, BIOMED ENVIRON SCI, V32, P438, DOI 10.3967/bes2019.058; Masters P.S., 2013, FIELDS VIROLOGY, P825; Palacios G, 2008, NEW ENGL J MED, V358, P991, DOI 10.1056/NEJMoa073785; Su S, 2016, TRENDS MICROBIOL, V24, P490, DOI 10.1016/j.tim.2016.03.003; Tan W, 2020, CHINA CDC WEEKLY, V2, P61; Weiss SR, 2011, ADV VIRUS RES, V81, P85, DOI 10.1016/B978-0-12-385885-6.00009-2; Wong G, 2015, CELL HOST MICROBE, V18, P398, DOI 10.1016/j.chom.2015.09.013; Zaki AM, 2012, NEW ENGL J MED, V367, P1814, DOI 10.1056/NEJMoa1211721; Zhong NS, 2003, LANCET, V362, P1353, DOI 10.1016/S0140-6736(03)14630-2	17	86	85	360	360	MASSACHUSETTS MEDICAL SOC	WALTHAM	WALTHAM WOODS CENTER, 860 WINTER ST,, WALTHAM, MA 02451-1413 USA	0028-4793	1533-4406		NEW ENGL J MED	N. Engl. J. Med.	FEB 20	2020	382	8					727	733		10.1056/NEJMoa2001017			7	Medicine, General & Internal	General & Internal Medicine	KQ7SF	WOS:000517119800008	31978945	Bronze			2020-04-01	
J	Cui, J; Li, F; Shi, ZL				Cui, Jie; Li, Fang; Shi, Zheng-Li			Origin and evolution of pathogenic coronaviruses	NATURE REVIEWS MICROBIOLOGY			English	Review							RESPIRATORY-SYNDROME-CORONAVIRUS; RECEPTOR-BINDING DOMAIN; SARS-LIKE CORONAVIRUSES; DIPEPTIDYL PEPTIDASE 4; CHINESE HORSESHOE BATS; TO-HUMAN TRANSMISSION; MERS-COV; DROMEDARY CAMELS; SPIKE PROTEIN; FUNCTIONAL RECEPTOR	Severe acute respiratory syndrome coronavirus (SARS-CoV) and Middle East respiratory syndrome coronavirus (MERS-CoV) are two highly transmissible and pathogenic viruses that emerged in humans at the beginning of the 21st century. Both viruses likely originated in bats, and genetically diverse coronaviruses that are related to SARS-CoV and MERS-CoV were discovered in bats worldwide. In this Review, we summarize the current knowledge on the origin and evolution of these two pathogenic coronaviruses and discuss their receptor usage; we also highlight the diversity and potential of spillover of bat-borne coronaviruses, as evidenced by the recent spillover of swine acute diarrhoea syndrome coronavirus (SADS-CoV) to pigs.	[Cui, Jie; Shi, Zheng-Li] Chinese Acad Sci, Wuhan Inst Virol, CAS Key Lab Special Pathogens & Biosafety, Wuhan, Hubei, Peoples R China; [Li, Fang] Univ Minnesota, Coll Vet Med, Dept Vet & Biomed Sci, St Paul, MN 55108 USA	Shi, ZL (reprint author), Chinese Acad Sci, Wuhan Inst Virol, CAS Key Lab Special Pathogens & Biosafety, Wuhan, Hubei, Peoples R China.	zlshi@wh.iov.cn	Causey, Douglas/O-8584-2019; ?, ??/A-1013-2013	Causey, Douglas/0000-0002-4302-1771; Cui, Jie/0000-0001-8176-9951; ?, ??/0000-0001-8089-163X	Strategic Priority Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [XDB29010000]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [31621061]; US National Institutes of Health (NIH) National Institute of Allergy and Infection Diseases [R01AI110964]; NIHUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [R01AI089728, R01AI110700]; CAS Pioneer Hundred Talents Program; Wuhan Institute of Virology (WIV) "One-Three-Five" Strategic Program [WIV-135-TP1]	This work was jointly funded by the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB29010000), the National Natural Science Foundation of China (31621061) and the US National Institutes of Health (NIH) National Institute of Allergy and Infection Diseases (R01AI110964) to Z.-L.S; NIH grants (R01AI089728 and R01AI110700) to F.L.; the CAS Pioneer Hundred Talents Program to J.C.; and the Wuhan Institute of Virology (WIV) "One-Three-Five" Strategic Program (WIV-135-TP1) to J.C. and Z.-L.S.	Alagaili AN, 2014, MBIO, V5, DOI 10.1128/mBio.00884-14; Anthony SJ, 2017, MBIO, V8, DOI [10.1128/mBio.00373-17, 10.1128/mbio.00373-17]; Anthony SJ, 2017, VIRUS EVOL, V3, DOI 10.1093/ve/vex012; Azhar EI, 2014, NEW ENGL J MED, V370, P2499, DOI 10.1056/NEJMoa1401505; Babcock GJ, 2004, J VIROL, V78, P4552, DOI 10.1128/JVI.78.9.4552-4560.2004; Balboni A, 2012, SCI WORLD J, DOI 10.1100/2012/989514; Barlan A, 2014, J VIROL, V88, P4953, DOI 10.1128/JVI.00161-14; Becker MM, 2008, P NATL ACAD SCI USA, V105, P19944, DOI 10.1073/pnas.0808116105; Brian DA, 2005, CURR TOP MICROBIOL, V287, P1, DOI 10.1007/3-540-26765-4_1; Chen CY, 2007, J INFECT DIS, V196, P405, DOI 10.1086/519166; Chen YQ, 2013, J VIROL, V87, P10777, DOI 10.1128/JVI.01756-13; Chu DKW, 2018, P NATL ACAD SCI USA, V115, P3144, DOI 10.1073/pnas.1718769115; Cockrell AS, 2014, J VIROL, V88, P5195, DOI 10.1128/JVI.03764-13; Corman VM, 2016, P NATL ACAD SCI USA, V113, P9864, DOI 10.1073/pnas.1604472113; Corman VM, 2014, EMERG INFECT DIS, V20, P1319, DOI 10.3201/eid2008.140596; Corman VM, 2014, J VIROL, V88, P11297, DOI 10.1128/JVI.01498-14; de Groot RJ, 2013, J VIROL, V87, P7790, DOI 10.1128/JVI.01244-13; de Wit E, 2016, NAT REV MICROBIOL, V14, P523, DOI 10.1038/nrmicro.2016.81; Drexler JF, 2014, ANTIVIR RES, V101, P45, DOI 10.1016/j.antiviral.2013.10.013; Drexler JF, 2010, J VIROL, V84, P11336, DOI 10.1128/JVI.00650-10; Drosten C, 2003, NEW ENGL J MED, V348, P1967, DOI 10.1056/NEJMoa030747; Du LY, 2013, J VIROL, V87, P9939, DOI 10.1128/JVI.01048-13; Dudas G, 2016, VIRUS EVOL, V2, DOI 10.1093/ve/vev023; Emsley P, 2004, ACTA CRYSTALLOGR D, V60, P2126, DOI 10.1107/S0907444904019158; Forni D, 2017, TRENDS MICROBIOL, V25, P35, DOI 10.1016/j.tim.2016.09.001; Fouchier RAM, 2003, NATURE, V423, P240, DOI 10.1038/423240a; Ge XY, 2013, NATURE, V503, P535, DOI 10.1038/nature12711; Gong L, 2017, EMERG INFECT DIS, V23, P1607, DOI 10.3201/eid2309.170915; Gouilh MA, 2011, INFECT GENET EVOL, V11, P1690, DOI 10.1016/j.meegid.2011.06.021; Graham RL, 2013, NAT REV MICROBIOL, V11, P836, DOI 10.1038/nrmicro3143; Guan Y, 2003, SCIENCE, V302, P276, DOI 10.1126/science.1087139; Guindon S, 2010, SYST BIOL, V59, P307, DOI 10.1093/sysbio/syq010; Haagmans BL, 2014, LANCET INFECT DIS, V14, P140, DOI 10.1016/S1473-3099(13)70690-X; Harcourt JL, 2018, ZOONOSES PUBLIC HLTH, V65, P749, DOI 10.1111/zph.12482; He BA, 2014, J VIROL, V88, P7070, DOI 10.1128/JVI.00631-14; He JF, 2004, SCIENCE, V303, P1666, DOI 10.1126/science.1092002; Hemida MG, 2013, EUROSURVEILLANCE, V18, P21, DOI 10.2807/1560-7917.ES2013.18.50.20659; Hemida MG, 2014, EMERG INFECT DIS, V20, P1231, DOI 10.3201/eid2007.140571; Hon CC, 2008, J VIROL, V82, P1819, DOI 10.1128/JVI.01926-07; Hu B, 2017, PLOS PATHOG, V13, DOI 10.1371/journal.ppat.1006698; Hu B, 2015, VIROL J, V12, DOI 10.1186/s12985-015-0422-1; Huang YW, 2013, MBIO, V4, DOI 10.1128/mBio.00737-13; Huynh J, 2012, J VIROL, V86, P12816, DOI 10.1128/JVI.00906-12; Ithete NL, 2013, EMERG INFECT DIS, V19, P1697, DOI 10.3201/eid1910.130946; Kan B, 2005, J VIROL, V79, P11892, DOI 10.1128/JVI.79.18.11892-11900.2005; Ksiazek TG, 2003, NEW ENGL J MED, V348, P1953, DOI 10.1056/NEJMoa030781; Lacroix A, 2017, INFECT GENET EVOL, V48, P10, DOI 10.1016/j.meegid.2016.11.029; Lai MMC, 1997, ADV VIRUS RES, V48, P1, DOI 10.1016/S0065-3527(08)60286-9; Lau SKP, 2005, P NATL ACAD SCI USA, V102, P14040, DOI 10.1073/pnas.0506735102; Lau SKP, 2007, VIROLOGY, V367, P428, DOI 10.1016/j.virol.2007.06.009; Lau SKP, 2018, J INFECT DIS, V218, P197, DOI 10.1093/infdis/jiy018; Lau SKP, 2013, J VIROL, V87, P8638, DOI 10.1128/JVI.01055-13; Lau SKP, 2010, J VIROL, V84, P2808, DOI 10.1128/JVI.02219-09; Le TM, 2007, FEBS J, V274, P4211, DOI 10.1111/j.1742-4658.2007.05947.x; Li F, 2005, SCIENCE, V309, P1864, DOI 10.1126/science.1116480; Li F., ADV EXPT MED BIOL, V581, P229; Li F, 2008, J VIROL, V82, P6984, DOI 10.1128/JVI.00442-08; Li F, 2006, J VIROL, V80, P6794, DOI 10.1128/JVI.02744-05; Li F, 2015, J VIROL, V89, P1954, DOI 10.1128/JVI.02615-14; Li F, 2013, ANTIVIR RES, V100, P246, DOI 10.1016/j.antiviral.2013.08.014; Li WD, 2005, SCIENCE, V310, P676, DOI 10.1126/science.1118391; Li WH, 2003, NATURE, V426, P450, DOI 10.1038/nature02145; Li WH, 2006, J VIROL, V80, P4211, DOI 10.1128/JVI.80.9.4211-4219.2006; Li WH, 2005, EMBO J, V24, P1634, DOI 10.1038/sj.emboj.7600640; Liang GD, 2004, EMERG INFECT DIS, V10, P1774, DOI 10.3201/eid1010.040445; Lin CM, 2016, VIRUS RES, V226, P20, DOI 10.1016/j.virusres.2016.05.023; Lin XD, 2017, VIROLOGY, V507, P1, DOI 10.1016/j.virol.2017.03.019; Liu C, 2015, J VIROL, V89, P6121, DOI 10.1128/JVI.00430-15; Liu L, 2007, J VIROL, V81, P4694, DOI 10.1128/JVI.02389-06; Lu GW, 2013, NATURE, V500, P227, DOI 10.1038/nature12328; Luo CM, 2018, J VIROL, V92, DOI 10.1128/JVI.00116-18; Mackay IM, 2015, VIROL J, V12, DOI 10.1186/s12985-015-0439-5; Simas PVM, 2015, EMERG INFECT DIS, V21, P729, DOI 10.3201/eid2104.141783; Masters P.S., 2013, FIELDS VIROLOGY, P825; Matthews KL, 2014, J GEN VIROL, V95, P874, DOI 10.1099/vir.0.062059-0; Memish ZA, 2013, EMERG INFECT DIS, V19, P1819, DOI 10.3201/eid1911.131172; Menachery VD, 2017, MBIO, V8, DOI 10.1128/mBio.00665-17; Menachery VD, 2016, P NATL ACAD SCI USA, V113, P3048, DOI 10.1073/pnas.1517719113; Menachery VD, 2015, NAT MED, V21, P1508, DOI 10.1038/nm.3985; Mou H, 2013, J VIROL, V87, P9379, DOI 10.1128/JVI.01277-13; Muller MA, 2015, LANCET INFECT DIS, V15, P559, DOI 10.1016/S1473-3099(15)70090-3; Muller MA, 2014, EMERG INFECT DIS, V20, P2093, DOI 10.3201/eid2012.141026; Nagy PD, 1997, VIROLOGY, V235, P1, DOI 10.1006/viro.1997.8681; Oostra M, 2007, J VIROL, V81, P13876, DOI 10.1128/JVI.01631-07; Paden CR, 2018, ZOONOSES PUBLIC HLTH, V65, P322, DOI 10.1111/zph.12435; Pan YF, 2017, VET MICROBIOL, V211, P15, DOI 10.1016/j.vetmic.2017.09.020; Peck KM, 2017, J VIROL, V91, DOI 10.1128/JVI.00534-17; Peck KM, 2015, J VIROL, V89, P4696, DOI 10.1128/JVI.03445-14; Perera RA, 2013, EUROSURVEILLANCE, V18, P8, DOI 10.2807/1560-7917.ES2013.18.36.20574; Perlman S, 2009, NAT REV MICROBIOL, V7, P439, DOI 10.1038/nrmicro2147; Qian ZH, 2013, AM J RESP CELL MOL, V48, P742, DOI 10.1165/rcmb.2012-0339OC; Qu XX, 2005, J BIOL CHEM, V280, P29588, DOI 10.1074/jbc.M500662200; Raj VS, 2014, EMERG INFECT DIS, V20, P1339, DOI 10.3201/eid2008.140663; Raj VS, 2013, NATURE, V495, P251, DOI 10.1038/nature12005; Ren W, 2008, J VIROL, V82, P1899, DOI 10.1128/JVI.01085-07; Ren W, 2006, J GEN VIROL, V87, P3355, DOI 10.1099/vir.0.82220-0; Reusken CBEM, 2013, LANCET INFECT DIS, V13, P859, DOI 10.1016/S1473-3099(13)70164-6; Rihtaric D, 2010, ARCH VIROL, V155, P507, DOI 10.1007/s00705-010-0612-5; Rowe CL, 1997, J VIROL, V71, P6183, DOI 10.1128/JVI.71.8.6183-6190.1997; Sabir JSM, 2016, SCIENCE, V351, P81, DOI 10.1126/science.aac8608; Saqib M, 2017, EMERG INFECT DIS, V23, P550, DOI 10.3201/eid2303.161285; Scobey T, 2013, P NATL ACAD SCI USA, V110, P16157, DOI 10.1073/pnas.1311542110; Sheahan T, 2008, J VIROL, V82, P2274, DOI 10.1128/JVI.02041-07; Song HD, 2005, P NATL ACAD SCI USA, V102, P2430, DOI 10.1073/pnas.0409608102; Su S, 2016, TRENDS MICROBIOL, V24, P490, DOI 10.1016/j.tim.2016.03.003; Sung SC, 2009, VIROLOGY, V387, P402, DOI 10.1016/j.virol.2009.02.021; Tao Y, 2017, J VIROL, V91, DOI 10.1128/JVI.01953-16; Tong SX, 2009, EMERG INFECT DIS, V15, P482, DOI 10.3201/eid1503.081013; Towler P, 2004, J BIOL CHEM, V279, P17996, DOI 10.1074/jbc.M311191200; Tu CC, 2004, EMERG INFECT DIS, V10, P2244, DOI 10.3201/eid1012.040520; van Doremalen N, 2014, J VIROL, V88, P9220, DOI 10.1128/JVI.00676-14; Wacharapluesadee S, 2015, VIROL J, V12, DOI 10.1186/s12985-015-0289-1; Wang L, 2018, TRENDS MICROBIOL, V26, P466, DOI 10.1016/j.tim.2018.03.001; Wang MN, 2016, VIROL SIN, V31, P78, DOI 10.1007/s12250-015-3703-3; Wang Ming, 2004, Zhonghua Liu Xing Bing Xue Za Zhi, V25, P503; Wang NS, 2013, CELL RES, V23, P986, DOI 10.1038/cr.2013.92; Wang N, 2018, VIROL SIN, V33, P104, DOI 10.1007/s12250-018-0012-7; Wang QH, 2014, CELL HOST MICROBE, V16, P328, DOI 10.1016/j.chom.2014.08.009; Wang YQ, 2015, MBIO, V6, DOI 10.1128/mBio.01280-15; Wong HH, 2018, VIROLOGY, V515, P165, DOI 10.1016/j.virol.2017.12.028; Wong SK, 2004, J BIOL CHEM, V279, P3197, DOI 10.1074/jbc.C300520200; Woo PCY, 2012, J VIROL, V86, P3995, DOI 10.1128/JVI.06540-11; Woo PCY, 2009, EXP BIOL MED, V234, P1117, DOI 10.3181/0903-MR-94; Wu KL, 2012, J BIOL CHEM, V287, P8904, DOI 10.1074/jbc.M111.325803; Wu KL, 2011, J VIROL, V85, P5331, DOI 10.1128/JVI.02274-10; Wu ZG, 2016, J INFECT DIS, V213, P579, DOI 10.1093/infdis/jiv476; Xu Hui-fang, 2004, Zhonghua Yufang Yixue Zazhi, V38, P81; Yang L, 2014, EMERG INFECT DIS, V20, P1260, DOI 10.3201/eid2007.140318; Yang L, 2013, EMERG INFECT DIS, V19, P989, DOI 10.3201/eid1906.121648; Yang XL, 2016, J VIROL, V90, P3253, DOI 10.1128/JVI.02582-15; Yang Y, 2015, SCI REP-UK, V5, DOI 10.1038/srep17554; Yang Y, 2014, P NATL ACAD SCI USA, V111, P12516, DOI 10.1073/pnas.1405889111; Yuan JF, 2010, J GEN VIROL, V91, P1058, DOI 10.1099/vir.0.016378-0; Yuan Y, 2017, NAT COMMUN, V8, DOI 10.1038/ncomms15092; Zaki AM, 2012, NEW ENGL J MED, V367, P1814, DOI 10.1056/NEJMoa1211721; Zeng LP, 2017, SCI CHINA LIFE SCI, V60, P1399, DOI 10.1007/s11427-017-9189-3; Zhang Z, 2016, SCI REP-UK, V6, DOI 10.1038/srep25049; Zhao ZM, 2004, BMC EVOL BIOL, V4, DOI 10.1186/1471-2148-4-21; Zhong NS, 2003, LANCET, V362, P1353, DOI 10.1016/S0140-6736(03)14630-2; Zhou P, 2018, NATURE, V556, P255, DOI 10.1038/s41586-018-0010-9; Zhou P, 2012, J GEN VIROL, V93, P275, DOI 10.1099/vir.0.033589-0	141	49	48	236	267	NATURE PUBLISHING GROUP	LONDON	MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND	1740-1526	1740-1534		NAT REV MICROBIOL	Nat. Rev. Microbiol.	MAR	2019	17	3					181	192		10.1038/s41579-018-0118-9			12	Microbiology	Microbiology	HL2CL	WOS:000458510700009	30531947	Other Gold	Y	N	2020-04-01	
J	Chen, Y; Liu, QY; Guo, DY				Chen, Yu; Liu, Qianyun; Guo, Deyin			Emerging coronaviruses: Genome structure, replication, and pathogenesis	JOURNAL OF MEDICAL VIROLOGY			English	Review						coronavirus; epidemiology; pathogenesis; respiratory tract; virus classification; zoonoses	RESPIRATORY SYNDROME-CORONAVIRUS; MURINE HEPATITIS-VIRUS; RNA VIRUS; IN-VITRO; SARS; PROTEIN; NSP12; DELTACORONAVIRUS; EXORIBONUCLEASE; IDENTIFICATION	The recent emergence of a novel coronavirus (2019-nCoV), which is causing an outbreak of unusual viral pneumonia in patients in Wuhan, a central city in China, is another warning of the risk of CoVs posed to public health. In this minireview, we provide a brief introduction of the general features of CoVs and describe diseases caused by different CoVs in humans and animals. This review will help understand the biology and potential risk of CoVs that exist in richness in wildlife such as bats.	[Chen, Yu; Liu, Qianyun] Wuhan Univ, Coll Life Sci, Modern Virol Res Ctr, State Key Lab Virol, Wuhan, Peoples R China; [Guo, Deyin] Sun Yat Sen Univ, Ctr Infect & Immun Study, Sch Med, Guangzhou 510080, Peoples R China	Guo, DY (reprint author), Sun Yat Sen Univ, Ctr Infect & Immun Study, Sch Med, Guangzhou 510080, Peoples R China.; Chen, Y (reprint author), Wuhan Univ, Coll Life Sci, Modern Virol Res Ctr, Wuhan 430072, Peoples R China.	chenyu@whu.edu.cn; guodeyin@mail.sysu.edu.cn			China National Science and Technology Major Project [2018ZX10733403]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81620108020, 81672008]; Shenzhen Science and Technology Program [KQTD20180411143323605]; Guangdong Zhujiang Talents Program	China National Science and Technology Major Project, Grant/Award Number: #2018ZX10733403; National Natural Science Foundation of China, Grant/Award Number: #81620108020 & #81672008; Shenzhen Science and Technology Program, Grant/Award Number: KQTD20180411143323605; Guangdong Zhujiang Talents Program, Grant/Award Number: 2017	Adedeji AO, 2016, MSPHERE, V1, DOI 10.1128/mSphere.00235-16; Ahn DG, 2012, ARCH VIROL, V157, P2095, DOI 10.1007/s00705-012-1404-x; Angelini MM, 2013, MBIO, V4, DOI 10.1128/mBio.00524-13; Beachboard DC, 2015, J VIROL, V89, P2080, DOI 10.1128/JVI.02776-14; Beniac DR, 2006, NAT STRUCT MOL BIOL, V13, P751, DOI 10.1038/nsmb1123; Bhardwaj K, 2006, J MOL BIOL, V361, P243, DOI 10.1016/j.jmb.2006.06.021; Bouvet M, 2014, J BIOL CHEM, V289, P25783, DOI 10.1074/jbc.M114.577353; Bouvet M, 2012, P NATL ACAD SCI USA, V109, P9372, DOI 10.1073/pnas.1201130109; Cauchemez S., 2013, Eurosurveillance, V18, P20503; Chan JFW, 2013, J INFECTION, V67, P606, DOI 10.1016/j.jinf.2013.09.029; Chang CK, 2006, J BIOMED SCI, V13, P59, DOI 10.1007/s11373-005-9035-9; Chen Y, 2016, VIROL SIN, V31, P3, DOI 10.1007/s12250-016-3726-4; Chen Y, 2011, PLOS PATHOG, V7, DOI 10.1371/journal.ppat.1002294; Chen Y, 2009, P NATL ACAD SCI USA, V106, P3484, DOI 10.1073/pnas.0808790106; Cheng KW, 2015, ANTIVIR RES, V115, P9, DOI 10.1016/j.antiviral.2014.12.011; Cinatl J, 2003, LANCET, V362, P293, DOI 10.1016/S0140-6736(03)13973-6; Cottam EM, 2014, AUTOPHAGY, V10, P1426, DOI 10.4161/auto.29309; Cui J, 2019, NAT REV MICROBIOL, V17, P181, DOI 10.1038/s41579-018-0118-9; Cui L, 2015, J VIROL, V89, P9029, DOI 10.1128/JVI.01331-15; de Wit E, 2016, NAT REV MICROBIOL, V14, P523, DOI 10.1038/nrmicro.2016.81; Decroly E, 2011, PLOS PATHOG, V7, DOI 10.1371/journal.ppat.1002059; DeDiego ML, 2007, J VIROL, V81, P1701, DOI 10.1128/JVI.01467-06; DELMAS B, 1990, J VIROL, V64, P5367, DOI 10.1128/JVI.64.11.5367-5375.1990; Deng XF, 2017, P NATL ACAD SCI USA, V114, pE4251, DOI 10.1073/pnas.1618310114; Eckerle LD, 2010, PLOS PATHOG, V6, DOI 10.1371/journal.ppat.1000896; Egloff MP, 2004, P NATL ACAD SCI USA, V101, P3792, DOI 10.1073/pnas.0307877101; Fang SG, 2008, VIROLOGY, V379, P175, DOI 10.1016/j.virol.2008.06.038; Fehr AR, 2015, METHODS MOL BIOL, V1282, P1, DOI 10.1007/978-1-4939-2438-7_1; Gadlage MJ, 2010, J VIROL, V84, P280, DOI 10.1128/JVI.01772-09; Gadlage MJ, 2008, J VIROL, V82, P11964, DOI 10.1128/JVI.01126-07; Ge XY, 2013, NATURE, V503, P535, DOI 10.1038/nature12711; Graham RL, 2013, NAT REV MICROBIOL, V11, P836, DOI 10.1038/nrmicro3143; Graham RL, 2005, J VIROL, V79, P13399, DOI 10.1128/JVI.79.21.13399-13411.2005; Hao W, 2017, PLOS PATHOG, V13, DOI 10.1371/journal.ppat.1006474; Huang C, 2011, PLOS PATHOG, V7, DOI 10.1371/journal.ppat.1002433; Hurst KR, 2009, J VIROL, V83, P7221, DOI 10.1128/JVI.00440-09; Hussain S, 2005, J VIROL, V79, P5288, DOI 10.1128/JVI.79.9.5288-5295.2005; Jia ZH, 2019, NUCLEIC ACIDS RES, V47, P6538, DOI 10.1093/nar/gkz409; Kirchdoerfer RN, 2019, NAT COMMUN, V10, DOI 10.1038/s41467-019-10280-3; Lei J, 2018, ANTIVIR RES, V149, P58, DOI 10.1016/j.antiviral.2017.11.001; Ma YY, 2015, P NATL ACAD SCI USA, V112, P9436, DOI 10.1073/pnas.1508686112; Mair-Jenkins J, 2015, J INFECT DIS, V211, P80, DOI 10.1093/infdis/jiu396; Simas PVM, 2015, EMERG INFECT DIS, V21, P729, DOI 10.3201/eid2104.141783; Masters PS, 2006, ADV VIRUS RES, V66, P193, DOI 10.1016/S0065-3527(06)66005-3; Minskaia E, 2006, P NATL ACAD SCI USA, V103, P5108, DOI 10.1073/pnas.0508200103; Nal B, 2005, J GEN VIROL, V86, P1423, DOI 10.1099/vir.0.80671-0; Neuman BW, 2011, J STRUCT BIOL, V174, P11, DOI 10.1016/j.jsb.2010.11.021; Nieto-Torres JL, 2014, PLOS PATHOG, V10, DOI 10.1371/journal.ppat.1004077; Ogando NS, 2019, FRONT MICROBIOL, V10, DOI 10.3389/fmicb.2019.01813; Perlman S, 2009, NAT REV MICROBIOL, V7, P439, DOI 10.1038/nrmicro2147; Sawicki SG, 2007, J VIROL, V81, P20, DOI 10.1128/JVI.01358-06; Serrano P, 2009, J VIROL, V83, P12998, DOI 10.1128/JVI.01253-09; Shi PD, 2019, VIRUS RES, V265, P57, DOI 10.1016/j.virusres.2019.03.005; Smith EC, 2013, PLOS PATHOG, V9, DOI 10.1371/journal.ppat.1003565; Snijder EJ, 2006, J VIROL, V80, P5927, DOI 10.1128/JVI.02501-05; Stobart CC, 2013, J VIROL, V87, P12611, DOI 10.1128/JVI.02050-13; Su S, 2016, TRENDS MICROBIOL, V24, P490, DOI 10.1016/j.tim.2016.03.003; Tanaka T, 2012, J VIROL, V86, P11128, DOI 10.1128/JVI.01700-12; Velthuis AJWT, 2012, NUCLEIC ACIDS RES, V40, P1737, DOI 10.1093/nar/gkr893; Velthuis AJWT, 2010, NUCLEIC ACIDS RES, V38, P203, DOI 10.1093/nar/gkp904; Wang LF, 2006, EMERG INFECT DIS, V12, P1834; Wang Y, 2015, J VIROL, V89, P8416, DOI 10.1128/JVI.00948-15; Woo PCY, 2012, J VIROL, V86, P3995, DOI 10.1128/JVI.06540-11; Zeng Z, 2018, J VIROL, V92, DOI 10.1128/JVI.00692-18; Zhai YJ, 2005, NAT STRUCT MOL BIOL, V12, P980, DOI 10.1038/nsmb999; Zhang LQ, 2018, J VIROL, V92, DOI 10.1128/JVI.00893-18; Zhou P, 2018, NATURE, V556, P255, DOI 10.1038/s41586-018-0010-9; Zhu XY, 2017, J VIROL, V91, DOI [10.1128/JVI.00003-17, 10.1128/jvi.00003-17]; Zhu XY, 2017, VIROLOGY, V502, P33, DOI 10.1016/j.virol.2016.12.005; Ziebuhr J, 2000, J GEN VIROL, V81, P853, DOI 10.1099/0022-1317-81-4-853	70	15	15	250	250	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0146-6615	1096-9071		J MED VIROL	J. Med. Virol.	APR	2020	92	4			SI		418	423		10.1002/jmv.25681		FEB 2020	6	Virology	Virology	KM6QU	WOS:000511535700001	31967327	Bronze			2020-04-01	
J	Zaher, NH; Mostafa, MI; Altaher, AY				Zaher, Nashwa Hafez; Mostafa, Mohammed Ismail; Altaher, Abdullah Yousef			Design, synthesis and molecular docking of novel triazole derivatives as potential CoV helicase inhibitors	ACTA PHARMACEUTICA			English	Article						triazole derivatives; anti-MERS-CoV activity; MERS-CoV helicase; docking	ACUTE RESPIRATORY SYNDROME; REPLICATION INHIBITOR; CORONAVIRUS; SARS; HEPATITIS	Middle East respiratory syndrome coronavirus (MERS-CoV) had emerged and spread because of the worldwide travel and inefficient healthcare provided for the infected patients in several countries. Herein we investigated the anti-MERS-CoV activity of newly synthesized sixteen halogenated triazole compounds through the inhibition of helicase activity using the FRET assay. All new compounds underwent justification for their target structures via microanalytical and spectral data. SAR studies were performed. Biological results revealed that the most potent compounds were 4-(cyclopent-1-en-3-ylamino)-5-(2-(4-iodophenyl)hydrazinyl)-4H-1,2,4-triazole-3-thiol (16) and 4-(cyclopent-1-en-3-ylamino)-5-[2-(4-chlorophenyl)hydrazinyl]-4H-1,2,4-triazole-3-thiol (12). In silico molecular docking of the most potent compounds was performed to the active binding site of MERS-CoV helicase nsp13. Molecular docking results are in agreement with experimental findings.	[Zaher, Nashwa Hafez] EAEA, NCRRT, Radiat Drug Res Dept, Cairo, Egypt; [Mostafa, Mohammed Ismail] Cairo Univ, Coll Vet Med, Dept Pharmacol, Gizah, Egypt; [Altaher, Abdullah Yousef] King Faisal Univ, Coll Vet Med, Dept Physiol Biochem & Pharmacol, Al Hasa, Saudi Arabia	Zaher, NH (reprint author), EAEA, NCRRT, Radiat Drug Res Dept, Cairo, Egypt.	nashwazah@hotmail.com			deanship of Scientific Research at King Faisal University [160002]	Authors would like to express their gratitude to Egyptian Atomic Energy Authority (EAEA), National Center for Radiation Research and Technology (NCRRT), Drug Radiation Research Department, drug chemistry lab, for the accommodation of the chemical synthetic part of this work. Authors would like also to extend their sincere appreciation to the deanship of Scientific Research at King Faisal University for its funding of this research through the research Project no. 160002.	Abdel-Hamid MK, 2007, BIOORGAN MED CHEM, V15, P6975, DOI 10.1016/j.bmc.2007.07.044; Adedeji AO, 2016, MSPHERE, V1, DOI 10.1128/mSphere.00235-16; Adedeji AO, 2014, CURR OPIN VIROL, V8, P45, DOI 10.1016/j.coviro.2014.06.002; Adedeji AO, 2014, ANTIMICROB AGENTS CH, V58, P4894, DOI 10.1128/AAC.02994-14; Adedeji AO, 2012, ANTIMICROB AGENTS CH, V56, P4718, DOI 10.1128/AAC.00957-12; Boguszewska-Chachulska AM, 2004, FEBS LETT, V567, P253, DOI 10.1016/j.febslet.2004.04.072; Dheer D, 2017, BIOORG CHEM, V71, P30, DOI 10.1016/j.bioorg.2017.01.010; Fadda AA, 2008, TURK J CHEM, V32, P259; Frick DN, 2006, CURR PHARM DESIGN, V12, P1315, DOI 10.2174/138161206776361147; Graham RL, 2013, NAT REV MICROBIOL, V11, P836, DOI 10.1038/nrmicro3143; He YW, 2014, EUR J MED CHEM, V76, P245, DOI 10.1016/j.ejmech.2014.02.029; Kao RY, 2004, CHEM BIOL, V11, P1293, DOI 10.1016/j.chembiol.2004.07.013; Peiris JSM, 2003, NEW ENGL J MED, V349, P2431, DOI 10.1056/NEJMra032498; Penttinen PM, 2013, EUROSURVEILLANCE, V18, P11, DOI 10.2807/1560-7917.ES2013.18.39.20596; Porter DJT, 1998, J BIOL CHEM, V273, P7390, DOI 10.1074/jbc.273.13.7390; Qian L, 2018, PROCEEDINGS OF INTERNATIONAL CONFERENCE ON ROBOTICS, CONTROL AND AUTOMATION ENGINEERING (RAE 2018) AND INTERNATIONAL CONFERENCE ON ADVANCED MECHANICAL AND ELECTRICAL ENGINEERING (AMEE 2018), P132, DOI 10.1145/3303714.3303721; Raj VS, 2013, NATURE, V495, P251, DOI 10.1038/nature12005; Rota PA, 2003, SCIENCE, V300, P1394, DOI 10.1126/science.1085952; Stockman LJ, 2006, PLOS MED, V3, P1525, DOI 10.1371/journal.pmed.0030343; Tanner JA, 2003, J BIOL CHEM, V278, P39578, DOI 10.1074/jbc.C300328200; Tanner JA, 2005, CHEM BIOL, V12, P303, DOI 10.1016/j.chembiol.2005.01.006; World Health Organization, 2018, MERS COV GLOB SUMM A; Wu ZY, 2014, CHEMMEDCHEM, V9, P1546, DOI 10.1002/cmdc.201400075; Zaki AM, 2012, NEW ENGL J MED, V367, P1814, DOI 10.1056/NEJMoa1211721; Zhao JY, 2012, BIOORG MED CHEM LETT, V22, P2321, DOI 10.1016/j.bmcl.2012.01.056	25	1	1	238	238	HRVATSKO FARMACEUTSKO DRUSTOV (HFD)-CROATION PHARMACEUTICAL SOC	ZAGREB	MASARYKOVA 2, 10000 ZAGREB, CROATIA	1330-0075	1846-9558		ACTA PHARMACEUT	Acta Pharm.	JUN	2020	70	2					145	159		10.2478/acph-2020-0024			15	Pharmacology & Pharmacy	Pharmacology & Pharmacy	KD6PH	WOS:000507986300002	31955138	DOAJ Gold			2020-04-01	
J	Hui, DS; Azhar, EI; Madani, TA; Ntoumi, F; Kock, R; Dar, O; Ippolito, G; Mchugh, TD; Memish, ZA; Drosten, C; Zumla, A; Petersen, E				Hui, David S.; Azhar, Esam I.; Madani, Tariq A.; Ntoumi, Francine; Kock, Richard; Dar, Osman; Ippolito, Giuseppe; Mchugh, Timothy D.; Memish, Ziad A.; Drosten, Christian; Zumla, Alimuddin; Petersen, Eskild			The continuing 2019-nCoV epidemic threat of novel coronaviruses to global health - The latest 2019 novel coronavirus outbreak in Wuhan, China	INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES			English	Editorial Material									[Hui, David S.] Chinese Univ Hong Kong, Prince Wales Hosp, Dept Med & Therapeut, Shatin, Hong Kong, Peoples R China; [Azhar, Esam I.] King Abdulaziz Univ, King Fahd Med Res Ctr, Special Infect Agents Unit, Fac Appl Med Sci, Jeddah, Saudi Arabia; [Azhar, Esam I.] King Abdulaziz Univ, Dept Med Lab Technol, Fac Appl Med Sci, Jeddah, Saudi Arabia; [Madani, Tariq A.] King Abdulaziz Univ, Dept Med, Fac Med, Jeddah, Saudi Arabia; [Ntoumi, Francine] Fdn Congolaise Rech Med, Brazzaville, Rep Congo; [Kock, Richard] Univ London, Royal Vet Coll, Hawkshead Lane, Hatfield, Herts, England; [Dar, Osman] Royal Inst Int Affairs, Chatham House Ctr Global Hlth Secur, London, England; [Ippolito, Giuseppe] Lazzaro Spallanzani Natl Inst Infect Dis IRCCS, Rome, Italy; [Mchugh, Timothy D.; Zumla, Alimuddin] UCL, Div Infect & Immun, Ctr Clin Microbiol, London, England; [Memish, Ziad A.] King Saud Med City, Minist Hlth, Res Ctr, Riyadh, Saudi Arabia; [Memish, Ziad A.] Alfaisal Univ, Coll Med, Riyadh, Saudi Arabia; [Memish, Ziad A.] Emory Univ, Rollins Sch Publ Hlth, Hubert Dept Global Hlth, Atlanta, GA 30322 USA; [Drosten, Christian] Charite Univ Med Berlin, Berlin, Germany; [Drosten, Christian] Free Univ Berlin, Berlin, Germany; [Drosten, Christian] Humboldt Univ, Berlin, Germany; [Drosten, Christian] Berlin Inst Hlth, Inst Virol, Berlin, Germany; [Drosten, Christian] German Ctr Infect Res, Associated Partner Charite, Berlin, Germany; [Zumla, Alimuddin] UCL Hosp NHS Fdn Trust, NIHR Biomed Res Ctr, London, England; [Petersen, Eskild] Minist Hlth, Directorate Gen Dis Surveillance & Control, Muscat, Oman; [Petersen, Eskild] Univ Aarhus, Inst Clin Med, Fac Hlth Sci, Aarhus, Denmark	Hui, DS (reprint author), Chinese Univ Hong Kong, Prince Wales Hosp, Dept Med & Therapeut, Shatin, Hong Kong, Peoples R China.	dschui@cuhk.edu.hk	Ribeiro, Nuno/AAH-2299-2020; Hui, David SC/O-2754-2015; McHugh, Timothy D/AAF-7231-2020	McHugh, Timothy D/0000-0003-4658-8594; Zumla, Alimuddin/0000-0002-5111-5735	European and Developing Countries Clinical Trials Partnership the EU Horizon 2020 Framework Programme for Research and Innovation	All authors have a specialist interest in emerging and reemerging pathogens. FN, RK, OD, GI, TDMc, CD and AZ are members of the Pan-African Network on Emerging and Re-emerging Infections (PANDORA-ID-NET) funded by the European and Developing Countries Clinical Trials Partnership the EU Horizon 2020 Framework Programme for Research and Innovation. AZ is a National Institutes of Health Research senior investigator. All authors declare no conflicts of interest.	[Anonymous], DIS OUTBREAK NE 0208; Azhar EI, 2019, INFECT DIS CLIN N AM, V33, P891, DOI 10.1016/j.idc.2019.08.001; Hui DSC, 2019, INFECT DIS CLIN N AM, V33, P869, DOI 10.1016/j.idc.2019.07.001; Parry J, 2020, BMJ-BRIT MED J, V368, DOI 10.1136/bmj.m56; WHO, 2019, MERS SIT UPD; World Health Organization, 2019, SARS SEV AC RESP SYN; Zumla A, 2016, INT J INFECT DIS, V47, P5, DOI 10.1016/j.ijid.2016.06.012	7	24	24	229	229	ELSEVIER SCI LTD	OXFORD	THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND	1201-9712	1878-3511		INT J INFECT DIS	Int. J. Infect. Dis.	FEB	2020	91						264	266		10.1016/j.ijid.2020.01.009			3	Infectious Diseases	Infectious Diseases	KH7YW	WOS:000510867100045	31953166	DOAJ Gold, Green Accepted			2020-04-01	
J	Chan, JFW; Kok, KH; Zhu, Z; Chu, H; To, KKW; Yuan, SF; Yuen, KY				Chan, Jasper Fuk-Woo; Kok, Kin-Hang; Zhu, Zheng; Chu, Hin; To, Kelvin Kai-Wang; Yuan, Shuofeng; Yuen, Kwok-Yung			Genomic characterization of the 2019 novel human-pathogenic coronavirus isolated from a patient with atypical pneumonia after visiting Wuhan	EMERGING MICROBES & INFECTIONS			English	Article						Coronavirus; Wuhan; SARS; emerging; genome; respiratory; virus; bioinformatics	RESPIRATORY SYNDROME CORONAVIRUS; PROTEIN; EXPRESSION	A mysterious outbreak of atypical pneumonia in late 2019 was traced to a seafood wholesale market in Wuhan of China. Within a few weeks, a novel coronavirus tentatively named as 2019 novel coronavirus (2019-nCoV) was announced by the World Health Organization. We performed bioinformatics analysis on a virus genome from a patient with 2019-nCoV infection and compared it with other related coronavirus genomes. Overall, the genome of 2019-nCoV has 89% nucleotide identity with bat SARS-like-CoVZXC21 and 82% with that of human SARS-CoV. The phylogenetic trees of their orf1a/b, Spike, Envelope, Membrane and Nucleoprotein also clustered closely with those of the bat, civet and human SARS coronaviruses. However, the external subdomain of Spike's receptor binding domain of 2019-nCoV shares only 40% amino acid identity with other SARS-related coronaviruses. Remarkably, its orf3b encodes a completely novel short protein. Furthermore, its new orf8 likely encodes a secreted protein with an alpha-helix, following with a beta-sheet(s) containing six strands. Learning from the roles of civet in SARS and camel in MERS, hunting for the animal source of 2019-nCoV and its more ancestral virus would be important for understanding the origin and evolution of this novel lineage B betacoronavirus. These findings provide the basis for starting further studies on the pathogenesis, and optimizing the design of diagnostic, antiviral and vaccination strategies for this emerging infection.	[Chan, Jasper Fuk-Woo; Kok, Kin-Hang; Chu, Hin; To, Kelvin Kai-Wang; Yuan, Shuofeng] Univ Hong Kong, State Key Lab Emerging Infect Dis, Pokfulam, Hong Kong, Peoples R China; [Chan, Jasper Fuk-Woo; To, Kelvin Kai-Wang; Yuen, Kwok-Yung] Univ Hong Kong, Shenzhen Hosp, Dept Clin Microbiol & Infect Control, Shenzhen, Guangdong, Peoples R China; [Chan, Jasper Fuk-Woo; Kok, Kin-Hang; Zhu, Zheng; Chu, Hin; To, Kelvin Kai-Wang; Yuan, Shuofeng; Yuen, Kwok-Yung] Univ Hong Kong, Li Ka Shing Fac Med, Dept Microbiol, Pokfulam, Hong Kong, Peoples R China; [Chan, Jasper Fuk-Woo; Kok, Kin-Hang; Chu, Hin; To, Kelvin Kai-Wang; Yuan, Shuofeng; Yuen, Kwok-Yung] Univ Hong Kong, Li Ka Shing Fac Med, Carol Yu Ctr Infect, Pokfulam, Hong Kong, Peoples R China	Kok, KH (reprint author), Univ Hong Kong, State Key Lab Emerging Infect Dis, Pokfulam, Hong Kong, Peoples R China.; Yuen, KY (reprint author), Univ Hong Kong, Shenzhen Hosp, Dept Clin Microbiol & Infect Control, Shenzhen, Guangdong, Peoples R China.; Kok, KH; Yuen, KY (reprint author), Univ Hong Kong, Li Ka Shing Fac Med, Dept Microbiol, Pokfulam, Hong Kong, Peoples R China.; Kok, KH; Yuen, KY (reprint author), Univ Hong Kong, Li Ka Shing Fac Med, Carol Yu Ctr Infect, Pokfulam, Hong Kong, Peoples R China.	kyyuen@hku.hk; kyyuen@hku.hk	Chan, Jasper Fuk-Woo/D-8007-2013	Chan, Jasper Fuk-Woo/0000-0001-6336-6657	Respiratory Viral Research Foundation Limited; Chow Sin Lan Charity Fund Limited, Chan Yin Chuen Memorial Charitable Foundation; Hong Kong Hainan Commercial Association South China Microbiology Research Fund; Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Diseases and Research Capability on Antimicrobial Resistance for Department of Health of the Hong Kong Special Administrative Region Government [T11/707/15]; Research Grants Council, Hong Kong Special Administrative RegionHong Kong Research Grants Council; Sanming Project of Medicine in Shenzhen, China [SZSM201911014]; Health Commission of Guangdong Province, China	This study was partly supported by the donations of Michael Seak-Kan Tong, Respiratory Viral Research Foundation Limited, Hui Ming, Hui Hoy and Chow Sin Lan Charity Fund Limited, Chan Yin Chuen Memorial Charitable Foundation, Marina Man-Wai Lee, and the Hong Kong Hainan Commercial Association South China Microbiology Research Fund; and funding from the Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Diseases and Research Capability on Antimicrobial Resistance for Department of Health of the Hong Kong Special Administrative Region Government; the ThemeBased Research Scheme (T11/707/15) of the Research Grants Council, Hong Kong Special Administrative Region; Sanming Project of Medicine in Shenzhen, China (No. SZSM201911014); and the High Level-Hospital Program, Health Commission of Guangdong Province, China.	Buchan DWA, 2019, NUCLEIC ACIDS RES, V47, pW402, DOI 10.1093/nar/gkz297; Chan JFW, 2015, CLIN MICROBIOL REV, V28, P465, DOI 10.1128/CMR.00102-14; Chan JFW, 2020, LANCET, V395, P514, DOI 10.1016/S0140-6736(20)30154-9; Chan JFW, 2013, TRENDS MICROBIOL, V21, P544, DOI 10.1016/j.tim.2013.05.005; Cheng VCC, 2007, CLIN MICROBIOL REV, V20, P660, DOI 10.1128/CMR.00023-07; FELSENSTEIN J, 1985, EVOLUTION, V39, P783, DOI 10.1111/j.1558-5646.1985.tb00420.x; Huang CL, 2020, LANCET, V395, P497, DOI 10.1016/S0140-6736(20)30183-5; Khan S, 2006, VIRUS RES, V122, P20, DOI 10.1016/j.virusres.2006.06.005; Kopecky-Bromberg SA, 2007, J VIROL, V81, P548, DOI 10.1128/JVI.01782-06; Kumar S, 2018, MOL BIOL EVOL, V35, P1547, DOI 10.1093/molbev/msy096; Lau SKP, 2015, J VIROL, V89, P10532, DOI 10.1128/JVI.01048-15; Oostra M, 2007, J VIROL, V81, P13876, DOI 10.1128/JVI.01631-07; Peiris JSM, 2003, LANCET, V361, P1319, DOI 10.1016/S0140-6736(03)13077-2; SAITOU N, 1987, MOL BIOL EVOL, V4, P406, DOI 10.1093/oxfordjournals.molbev.a040454; Shi CS, 2019, CELL DEATH DISCOV, V5, DOI 10.1038/s41420-019-0181-7; Song HD, 2005, P NATL ACAD SCI USA, V102, P2430, DOI 10.1073/pnas.0409608102; South China Morning Post, S CHINA MORNING POST; Wang QH, 2014, CELL HOST MICROBE, V16, P328, DOI 10.1016/j.chom.2014.08.009; Woo PCY, 2005, J VIROL, V79, P884, DOI 10.1128/JVI.79.2.884-895.2005; World Health Organization, NOV COR; World Health Organization, NOV COR THAIL EX CHI; Xia S, 2019, SCI ADV, V5, DOI 10.1126/sciadv.aav4580; Yang D, 2015, VIRUS RES, V206, P120, DOI 10.1016/j.virusres.2015.02.025; Yeung ML, 2016, NAT MICROBIOL, V1, DOI [10.1038/NMICROBIOL.2016.4, 10.1038/nmicrobiol.2016.4]; Yount B, 2005, J VIROL, V79, P14909, DOI 10.1128/JVI.79.23.14909-14922.2005; Zhou P, 2012, J GEN VIROL, V93, P275, DOI 10.1099/vir.0.033589-0; Zuckerkandl E, 1965, EVOLVING GENES PROTE, P97, DOI DOI 10.1016/B978-1-4832-2734-4.50017-6	27	9	9	185	185	TAYLOR & FRANCIS LTD	ABINGDON	2-4 PARK SQUARE, MILTON PARK, ABINGDON OR14 4RN, OXON, ENGLAND		2222-1751		EMERG MICROBES INFEC	Emerg. Microbes Infect.	JAN 1	2020	9	1					221	236		10.1080/22221751.2020.1719902			16	Immunology; Microbiology	Immunology; Microbiology	KH1BL	WOS:000510381600001	31987001	DOAJ Gold, Green Published			2020-04-01	
J	Zhang, NR; Wang, LL; Deng, XQ; Liang, RY; Su, M; He, C; Hu, LF; Su, YD; Ren, J; Yu, F; Du, LY; Jiang, SB				Zhang, Naru; Wang, Lili; Deng, Xiaoqian; Liang, Ruiying; Su, Meng; He, Chen; Hu, Lanfang; Su, Yudan; Ren, Jing; Yu, Fei; Du, Lanying; Jiang, Shibo			Recent advances in the detection of respiratory virus infection in humans	JOURNAL OF MEDICAL VIROLOGY			English	Review						adenovirus; coronavirus; diagnostic methods; influenza virus; respiratory syncytial virus; respiratory viral infection; rhinovirus	REVERSE TRANSCRIPTION-PCR; ISOTHERMAL AMPLIFICATION ASSAY; SYNCYTIAL VIRUS; RAPID DIAGNOSIS; MOLECULAR DIAGNOSIS; CLINICAL VALIDATION; INFLUENZA; IDENTIFICATION; CLASSIFICATION; IMMUNOASSAY	Respiratory tract viral infection caused by viruses or bacteria is one of the most common diseases in human worldwide, while those caused by emerging viruses, such as the novel coronavirus, 2019-nCoV that caused the pneumonia outbreak in Wuhan, China most recently, have posed great threats to global public health. Identification of the causative viral pathogens of respiratory tract viral infections is important to select an appropriate treatment, save people's lives, stop the epidemics, and avoid unnecessary use of antibiotics. Conventional diagnostic tests, such as the assays for rapid detection of antiviral antibodies or viral antigens, are widely used in many clinical laboratories. With the development of modern technologies, new diagnostic strategies, including multiplex nucleic acid amplification and microarray-based assays, are emerging. This review summarizes currently available and novel emerging diagnostic methods for the detection of common respiratory viruses, such as influenza virus, human respiratory syncytial virus, coronavirus, human adenovirus, and human rhinovirus. Multiplex assays for simultaneous detection of multiple respiratory viruses are also described. It is anticipated that such data will assist researchers and clinicians to develop appropriate diagnostic strategies for timely and effective detection of respiratory virus infections.	[Zhang, Naru] Zhejiang Univ City Coll, Sch Med, Dept Clin Med, Hangzhou, Peoples R China; [Wang, Lili] Hebei Agr Univ, Res Ctr Chinese Jujube, State Key Lab North China Crop Improvement & Regu, Baoding, Peoples R China; [Deng, Xiaoqian; Liang, Ruiying; Su, Meng; He, Chen; Hu, Lanfang; Su, Yudan; Ren, Jing; Yu, Fei] Hebei Agr Univ, Coll Life & Sci, State Key Lab North China Crop Improvement & Regu, Baoding, Peoples R China; [Du, Lanying; Jiang, Shibo] New York Blood Ctr, Lindsley F Kimball Res Inst, New York, NY 10065 USA; [Jiang, Shibo] Fudan Univ, Key Lab Med Mol Virol MOE NHC CAMS, Sch Basic Med Sci, Shanghai Med Coll, Shanghai, Peoples R China	Yu, F (reprint author), Hebei Agr Univ, Coll Life & Sci, State Key Lab North China Crop Improvement & Regu, Baoding, Peoples R China.; Du, LY; Jiang, SB (reprint author), New York Blood Ctr, Lindsley F Kimball Res Inst, New York, NY 10065 USA.	shmyf@hebau.edu.cn; ldu@nybc.org; shibojiang@fudan.edu.cn	Yu, Fei/K-4953-2019	Yu, Fei/0000-0001-6332-4326	Hebei Province's Program for Talents Returning from Studying Overseas [CN201707]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81974302, 81601761]; Hebei Agricultural University [ZD2016026, YJ201843]; Program for Youth Talent of Higher Learning Institutions of Hebei Province [BJ2018045]	Hebei Province's Program for Talents Returning from Studying Overseas, Grant/Award Number: CN201707; National Natural Science Foundation of China, Grant/Award Numbers: 81974302, 81601761; starting grant from Hebei Agricultural University, Grant/Award Numbers: ZD2016026, YJ201843; Program for Youth Talent of Higher Learning Institutions of Hebei Province, Grant/Award Number: BJ2018045	Abdel-Moneim AS, 2017, MOL CELL PROBE, V33, P16, DOI 10.1016/j.mcp.2017.02.006; Azar MM, 2018, J CLIN MICROBIOL, V56, DOI [10.1128/JCM.00367-18, 10.1128/jcm.00367-18]; Bentzen EL, 2005, NANO LETT, V5, P591, DOI 10.1021/nl048073u; Bhadra S, 2015, PLOS ONE, V10, DOI 10.1371/journal.pone.0123126; Bhandary R, 2016, J CLIN DIAGN RES, V10, pDC10, DOI 10.7860/JCDR/2016/17530.8044; BinSaeed AA, 2014, SAUDI MED J, V35, P277; Bochkov YA, 2014, J CLIN MICROBIOL, V52, P2461, DOI 10.1128/JCM.00075-14; Borek AP, 2006, J CLIN MICROBIOL, V44, P1105, DOI 10.1128/JCM.44.3.1105-1107.2006; Chan JFW, 2015, J CLIN MICROBIOL, V53, P2722, DOI 10.1128/JCM.01224-15; Chen C, 2018, DIAGN MICR INFEC DIS, V90, P90, DOI 10.1016/j.diagmicrobio.2017.10.005; Chen JHK, 2016, J CLIN MICROBIOL, V54, P1820, DOI 10.1128/JCM.00517-16; Chen M, 2015, PLOS ONE, V10, DOI 10.1371/journal.pone.0121375; Chen YX, 2016, J INFECTION, V73, P82, DOI 10.1016/j.jinf.2016.04.014; Corman VM, 2012, EUROSURVEILLANCE, V17; Deng Jikui, 2013, Virologica Sinica, V28, P97, DOI 10.1007/s12250-013-3312-y; Do DH, 2010, J MOL DIAGN, V12, P102, DOI 10.2353/jmoldx.2010.090071; Dou YH, 2018, MOL MED REP, V18, P2889, DOI 10.3892/mmr.2018.9253; Eboigbodin KE, 2017, BMC INFECT DIS, V17, DOI 10.1186/s12879-017-2227-x; Echenique IA, 2013, PLOS ONE, V8, DOI 10.1371/journal.pone.0060845; Falsey AR, 2002, J CLIN MICROBIOL, V40, P817, DOI 10.1128/JCM.40.3.817-820.2002; Fendrick AM, 2003, ARCH INTERN MED, V163, P487, DOI 10.1001/archinte.163.4.487; Feng ZS, 2018, VIROL J, V15, DOI 10.1186/s12985-018-1061-0; Go YY, 2017, J MOL DIAGN, V19, P817, DOI 10.1016/j.jmoldx.2017.06.007; Hashemzadeh MS, 2016, IRAN RED CRESCENT ME, V18, DOI 10.5812/ircmj.23874; Heim A, 2003, J MED VIROL, V70, P228, DOI 10.1002/jmv.10382; Hu AZ, 2003, J CLIN MICROBIOL, V41, P149, DOI 10.1128/JCM.41.1.149-154.2003; Hu B, 2015, VIROL J, V12, DOI 10.1186/s12985-015-0422-1; Huang P, 2018, FRONT MICROBIOL, V9, DOI 10.3389/fmicb.2018.01101; Huang T, 2009, PLOS ONE, V4, DOI 10.1371/journal.pone.0006355; Hung HM, 2019, J MICROBIOL IMMUNOL, V52, P233, DOI 10.1016/j.jmii.2018.08.009; Huzly D, 2016, ARCH VIROL, V161, P2417, DOI 10.1007/s00705-016-2930-8; Imai K, 2018, FRONT MICROBIOL, V9, DOI 10.3389/fmicb.2018.02748; Imai M, 2007, J VIROL METHODS, V141, P173, DOI 10.1016/j.jviromet.2006.12.004; Imai M, 2006, VACCINE, V24, P6679, DOI 10.1016/j.vaccine.2006.05.046; Kim MN, 2016, ANN LAB MED, V36, P450, DOI 10.3343/alm.2016.36.5.450; Koo B, 2018, BIOSENS BIOELECTRON, V119, P79, DOI 10.1016/j.bios.2018.08.007; Kubo T, 2010, J CLIN MICROBIOL, V48, P728, DOI 10.1128/JCM.01481-09; Li J, 2012, BMC INFECT DIS, V12, DOI 10.1186/1471-2334-12-189; Lim XF, 2019, J GEN VIROL, V100, P1363, DOI 10.1099/jgv.0.001307; Lion T, 2014, CLIN MICROBIOL REV, V27, P441, DOI 10.1128/CMR.00116-13; Loeffelholz MJ, 2011, J CLIN MICROBIOL, V49, P4083, DOI 10.1128/JCM.05010-11; Lu XY, 2014, J CLIN MICROBIOL, V52, P67, DOI 10.1128/JCM.02533-13; Madisch I, 2006, J MED VIROL, V78, P1210, DOI 10.1002/jmv.20683; Mahony JB, 2011, CRIT REV CL LAB SCI, V48, P217, DOI 10.3109/10408363.2011.640976; McIntyre CL, 2013, J GEN VIROL, V94, P1791, DOI 10.1099/vir.0.053686-0; Myatt TA, 2003, BMC PUBLIC HEALTH, V3, DOI 10.1186/1471-2458-3-5; Nakamura H, 2018, J MED VIROL, V90, P873, DOI 10.1002/jmv.25032; Nakauchi M, 2014, J VIROL METHODS, V204, P101, DOI 10.1016/j.jviromet.2014.03.028; Nascimento MS, 2010, CLINICS, V65, P1133, DOI 10.1590/S1807-59322010001100014; Noh JY, 2018, ARCH VIROL, V163, P819, DOI 10.1007/s00705-017-3677-6; Canela LNP, 2018, BRAZ J INFECT DIS, V22, P402, DOI 10.1016/j.bjid.2018.09.001; Poon LLM, 2005, J CLIN MICROBIOL, V43, P427, DOI 10.1128/JCM.43.1.427-430.2005; Rochelet M, 2012, TALANTA, V100, P139, DOI 10.1016/j.talanta.2012.07.088; Sam SS, 2018, J CLIN MICROBIOL, V56, DOI [10.1128/jcm.00787-18, 10.1128/JCM.00787-18]; Sato M, 2005, J CLIN MICROBIOL, V43, P36, DOI 10.1128/JCM.43.1.36-40.2005; Schibler M, 2012, J CLIN MICROBIOL, V50, P2868, DOI 10.1128/JCM.06752-11; Sears MR, 2008, J ALLERGY CLIN IMMUN, V122, P662, DOI 10.1016/j.jaci.2008.08.003; Selvarangan R, 2008, DIAGN MICR INFEC DIS, V62, P157, DOI 10.1016/j.diagmicrobio.2008.05.005; Shimada Y, 2004, J CLIN MICROBIOL, V42, P1577, DOI 10.1128/JCM.42.4.1577-1584.2004; Shirato K, 2018, J VIROL METHODS, V258, P41, DOI 10.1016/j.jviromet.2018.05.006; Shirato K, 2014, VIROL J, V11, DOI 10.1186/1743-422X-11-139; Simmonds P, 2010, J GEN VIROL, V91, P2409, DOI 10.1099/vir.0.023994-0; Simpson R, 2003, J MED VIROL, V70, P258, DOI 10.1002/jmv.10386; Slinger R, 2004, J CLIN MICROBIOL, V42, P3731, DOI [10.1128/JCM.42.8.3731-3733.2004, 10.1128/jcm.42.8.3731-3733.2004]; Sullender WM, 2000, CLIN MICROBIOL REV, V13, P1, DOI 10.1128/CMR.13.1.1-15.2000; Tripp RA, 2007, INT J NANOMED, V2, P117, DOI 10.2147/nano.2007.2.1.117; Trombetta Valentina K, 2018, Hawaii J Med Public Health, V77, P226; Turunen R, 2016, J MED VIROL, V88, P2059, DOI 10.1002/jmv.24587; Vemula SV, 2016, VIRUSES-BASEL, V8, DOI 10.3390/v8040096; Wang N, 2019, VIRUSES-BASEL, V11, DOI 10.3390/v11040379; Wong SSY, 2018, VIROL J, V15, DOI 10.1186/s12985-018-1059-7; Zaki AM, 2012, NEW ENGL J MED, V367, P1814, DOI 10.1056/NEJMoa1211721; Zhang NR, 2015, MICROBES INFECT, V17, P123, DOI 10.1016/j.micinf.2014.12.006; Zhang PH, 2014, J CLIN MICROBIOL, V52, P4385, DOI 10.1128/JCM.02635-14; Zhang QW, 2017, EMERG MICROBES INFEC, V6, DOI 10.1038/emi.2017.78; Zhao JQ, 2015, EMERG INFECT DIS, V21, P400, DOI 10.3201/eid2103.141169; Zhao JQ, 2012, VIRUSES-BASEL, V4, P3012, DOI 10.3390/v4113012; Zhao JQ, 2010, BMC BIOTECHNOL, V10, DOI 10.1186/1472-6750-10-74; Zhao L, 2019, DIAGN MICR INFEC DIS, V93, P101, DOI 10.1016/j.diagmicrobio.2018.09.001	79	7	7	182	182	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0146-6615	1096-9071		J MED VIROL	J. Med. Virol.	APR	2020	92	4			SI		408	417		10.1002/jmv.25674		FEB 2020	10	Virology	Virology	KM6QU	WOS:000510795900001	31944312	Bronze			2020-04-01	
J	Lu, RJ; Zhao, X; Li, J; Niu, PH; Yang, B; Wu, HL; Wang, WL; Song, H; Huang, BY; Zhu, N; Bi, YH; Ma, XJ; Zhan, FX; Wang, L; Hu, T; Zhou, H; Hu, ZH; Zhou, WM; Zhao, L; Chen, J; Meng, Y; Wang, J; Lin, Y; Yuan, JY; Xie, ZH; Ma, JM; Liu, WJ; Wang, DY; Xu, WB; Holmes, EC; Gao, GF; Wu, GZ; Chen, WJ; Shi, WF; Tan, WJ				Lu, Roujian; Zhao, Xiang; Li, Juan; Niu, Peihua; Yang, Bo; Wu, Honglong; Wang, Wenling; Song, Hao; Huang, Baoying; Zhu, Na; Bi, Yuhai; Ma, Xuejun; Zhan, Faxian; Wang, Liang; Hu, Tao; Zhou, Hong; Hu, Zhenhong; Zhou, Weimin; Zhao, Li; Chen, Jing; Meng, Yao; Wang, Ji; Lin, Yang; Yuan, Jianying; Xie, Zhihao; Ma, Jinmin; Liu, William J.; Wang, Dayan; Xu, Wenbo; Holmes, Edward C.; Gao, George F.; Wu, Guizhen; Chen, Weijun; Shi, Weifeng; Tan, Wenjie			Genomic characterisation and epidemiology of 2019 novel coronavirus: implications for virus origins and receptor binding	LANCET			English	Article							MERS-COV; SARS CORONAVIRUS; DOMAIN; TOOL	Background In late December, 2019, patients presenting with viral pneumonia due to an unidentified microbial agent were reported in Wuhan, China. A novel coronavirus was subsequently identified as the causative pathogen, provisionally named 2019 novel coronavirus (2019-nCoV). As of Jan 26, 2020, more than 2000 cases of 2019-nCoV infection have been confirmed, most of which involved people living in or visiting Wuhan, and human-to-human transmission has been confirmed. Methods We did next-generation sequencing of samples from bronchoalveolar lavage fluid and cultured isolates from nine inpatients, eight of whom had visited the Huanan seafood market in Wuhan. Complete and partial 2019-nCoV genome sequences were obtained from these individuals. Viral contigs were connected using Sanger sequencing to obtain the full-length genomes, with the terminal regions determined by rapid amplification of cDNA ends. Phylogenetic analysis of these 2019-nCoV genomes and those of other coronaviruses was used to determine the evolutionary history of the virus and help infer its likely origin. Homology modelling was done to explore the likely receptor-binding properties of the virus. Findings The ten genome sequences of 2019-nCoV obtained from the nine patients were extremely similar, exhibiting more than 99.98% sequence identity. Notably, 2019-nCoV was closely related (with 88% identity) to two bat-derived severe acute respiratory syndrome (SARS)-like coronaviruses, bat-SL-CoVZC45 and bat-SL-CoVZXC21, collected in 2018 in Zhoushan, eastern China, but were more distant from SARS-CoV (about 79%) and MERS-CoV (about 50%). Phylogenetic analysis revealed that 2019-nCoV fell within the subgenus Sarbecovirus of the genus Betacoronavirus, with a relatively long branch length to its closest relatives bat-SL-CoVZC45 and bat-SL-CoVZXC21, and was genetically distinct from SARS-CoV. Notably, homology modelling revealed that 2019-nCoV had a similar receptor-binding domain structure to that of SARS-CoV, despite amino acid variation at some key residues. Interpretation 2019-nCoV is sufficiently divergent from SARS-CoV to be considered a new human-infecting betacoronavirus. Although our phylogenetic analysis suggests that bats might be the original host of this virus, an animal sold at the seafood market in Wuhan might represent an intermediate host facilitating the emergence of the virus in humans. Importantly, structural analysis suggests that 2019-nCoV might be able to bind to the angiotensinconverting enzyme 2 receptor in humans. The future evolution, adaptation, and spread of this virus warrant urgent investigation.	[Lu, Roujian; Zhao, Xiang; Niu, Peihua; Wang, Wenling; Huang, Baoying; Zhu, Na; Ma, Xuejun; Zhou, Weimin; Zhao, Li; Meng, Yao; Wang, Ji; Liu, William J.; Wang, Dayan; Xu, Wenbo; Gao, George F.; Wu, Guizhen; Tan, Wenjie] Chinese Ctr Dis Control & Prevent, Natl Inst Viral Dis Control & Prevent, NHC Key Lab Biosafety, Beijing 102206, Peoples R China; [Li, Juan; Zhou, Hong; Shi, Weifeng] Shandong First Med Univ & Shandong Acad Med Sci, Key Lab Etiol & Epidemiol Emerging Infect Dis Uni, Tai An 271000, Shandong, Peoples R China; [Yang, Bo; Zhan, Faxian] Hubei Prov Ctr Dis Control & Prevent, Div Viral Dis Detect, Wuhan, Peoples R China; [Wu, Honglong; Lin, Yang; Yuan, Jianying; Xie, Zhihao; Ma, Jinmin; Chen, Weijun] BGI PathoGenesis Pharmaceut Technol, Shenzhen, Peoples R China; [Song, Hao] Chinese Acad Sci, Beijing Inst Life Sci, RNIH, Beijing, Peoples R China; [Bi, Yuhai; Wang, Liang; Gao, George F.] Chinese Acad Sci, Inst Microbiol, Key Lab Pathogen Microbiol & Immunol, Beijing, Peoples R China; [Bi, Yuhai; Wang, Liang; Gao, George F.] Chinese Acad Sci, CAS TWAS Ctr Excellence Emerging Infect Dis CEEID, Ctr Influenza Res & Early Warning CASCIRE, Beijing, Peoples R China; [Hu, Zhenhong] Peoples Liberat Army Gen Hosp, Cent Theater, Wuhan, Peoples R China; [Chen, Jing; Tan, Wenjie] Wenzhou Med Univ, Minist Educ, Key Lab Lab Med, Wenzhou, Peoples R China; [Chen, Jing; Tan, Wenjie] Wenzhou Med Univ, Sch Lab Med & Life Sci, Inst Med Virol, Zhejiang Prov Key Lab Med Genet, Wenzhou, Peoples R China; [Holmes, Edward C.] Univ Sydney, Sch Life & Environm Sci, Marie Bashir Inst Infect Dis & Biosecur, Sydney, NSW, Australia; [Holmes, Edward C.] Univ Sydney, Sch Med Sci, Sydney, NSW, Australia; [Shi, Weifeng] Shandong First Med Univ, Shandong Prov Qianfoshan Hosp, Affiliated Hosp 1, Jinan, Peoples R China; [Tan, Wenjie] Chinese Acad Sci, Ctr Biosafety Mega Sci, Beijing, Peoples R China	Tan, WJ (reprint author), Chinese Ctr Dis Control & Prevent, Natl Inst Viral Dis Control & Prevent, NHC Key Lab Biosafety, Beijing 102206, Peoples R China.; Shi, WF (reprint author), Shandong First Med Univ & Shandong Acad Med Sci, Key Lab Etiol & Epidemiol Emerging Infect Dis Uni, Tai An 271000, Shandong, Peoples R China.	wfshi@sdfmu.edu.cn; tanwj@ivdc.chinacdc.cn			National Key Research and Development Program of China; National Major Project for Control and Prevention of Infectious Disease in China; Chinese Academy of SciencesChinese Academy of Sciences; Shandong First Medical University	National Key Research and Development Program of China, National Major Project for Control and Prevention of Infectious Disease in China, Chinese Academy of Sciences, Shandong First Medical University.	ALAGAILI AN, 2014, MBIO, V5, DOI DOI 10.1128/MBIO.00884-14; Cavanagh D, 2007, VET RES, V38, P281, DOI 10.1051/vetres:2006055; Chan JFW, 2020, LANCET, V395, P514, DOI 10.1016/S0140-6736(20)30154-9; Chan-Yeung M, 2003, RESPIROLOGY, V8, pS9, DOI 10.1046/j.1440-1843.2003.00518.x; Guan Y, 2003, SCIENCE, V302, P276, DOI 10.1126/science.1087139; He YX, 2004, BIOCHEM BIOPH RES CO, V324, P773, DOI 10.1016/j.bbrc.2004.09.106; Hu D, 2018, EMERG MICROBES INFEC, V7, DOI 10.1038/s41426-018-0155-5; Huang CL, 2020, LANCET, V395, P497, DOI 10.1016/S0140-6736(20)30183-5; Ismail MM, 2003, AVIAN DIS, V47, P515, DOI 10.1637/5917; Lee JY, 2017, BMC INFECT DIS, V17, DOI 10.1186/s12879-017-2576-5; Lee J, 2016, J THEOR BIOL, V408, P118, DOI 10.1016/j.jtbi.2016.08.009; Li F, 2005, SCIENCE, V309, P1864, DOI 10.1126/science.1116480; Li F, 2016, ANNU REV VIROL, V3, P237, DOI 10.1146/annurev-virology-110615-042301; Li F, 2012, J VIROL, V86, P2856, DOI 10.1128/JVI.06882-11; Li H, 2009, BIOINFORMATICS, V25, P1754, DOI 10.1093/bioinformatics/btp324; Lole KS, 1999, J VIROL, V73, P152, DOI 10.1128/JVI.73.1.152-160.1999; Lu GW, 2015, TRENDS MICROBIOL, V23, P468, DOI 10.1016/j.tim.2015.06.003; Lu GW, 2013, NATURE, V500, P227, DOI 10.1038/nature12328; Marchler-Bauer A, 2017, NUCLEIC ACIDS RES, V45, pD200, DOI 10.1093/nar/gkw1129; Nakamura T, 2018, BIOINFORMATICS, V34, P2490, DOI 10.1093/bioinformatics/bty121; Niu PH, 2016, VIROL SIN, V31, P85, DOI 10.1007/s12250-015-3653-9; Nurk Sergey, 2013, Research in Computational Molecular Biology. 17th Annual International Conference (RECOMB 2013). Proceedings, P158, DOI 10.1007/978-3-642-37195-0_13; P Zhou, 2020, BIORXIV, DOI [10.1101/2020.01.22.914952., DOI 10.1101/2020.01.22.914952, 10.1101/2020.01.22.914952]; Pan M, 2016, J INFECTION, V72, P52, DOI 10.1016/j.jinf.2015.06.009; Peiris JSM, 2004, NAT MED, V10, pS88, DOI 10.1038/nm1143; Prabakaran P, 2006, J BIOL CHEM, V281, P15829, DOI 10.1074/jbc.M600697200; Stamatakis A, 2014, BIOINFORMATICS, V30, P1312, DOI 10.1093/bioinformatics/btu033; Su S, 2016, TRENDS MICROBIOL, V24, P490, DOI 10.1016/j.tim.2016.03.003; Tan W, 2020, CHINA CDC WEEKLY, V2, P61; Wang NS, 2013, CELL RES, V23, P986, DOI 10.1038/cr.2013.92; Wang QH, 2016, ANTIVIR RES, V133, P165, DOI 10.1016/j.antiviral.2016.07.015; Wang QH, 2014, CELL HOST MICROBE, V16, P328, DOI 10.1016/j.chom.2014.08.009; Waterhouse A, 2018, NUCLEIC ACIDS RES, V46, pW296, DOI 10.1093/nar/gky427; Zaki AM, 2012, NEW ENGL J MED, V367, P1814, DOI 10.1056/NEJMoa1211721; Zhao YA, 2012, BIOINFORMATICS, V28, P125, DOI 10.1093/bioinformatics/btr595; Zhou P, 2018, NATURE, V556, P255, DOI 10.1038/s41586-018-0010-9; Zhu N, 2020, NEW ENGL J MED, V382, P727, DOI 10.1056/NEJMoa2001017	37	36	35	181	181	ELSEVIER SCIENCE INC	NEW YORK	STE 800, 230 PARK AVE, NEW YORK, NY 10169 USA	0140-6736	1474-547X		LANCET	Lancet	FEB 22	2020	395	10224					565	574		10.1016/S0140-6736(20)30251-8			10	Medicine, General & Internal	General & Internal Medicine	KN5AJ	WOS:000514849400027	32007145	Bronze			2020-04-01	
J	Chan, JFW; Yuan, SF; Kok, KH; To, KKW; Chu, H; Yang, J; Xing, FF; Liu, JL; Yip, CCY; Poon, RWS; Tsoi, HW; Lo, SKF; Chan, KH; Poon, VKM; Chan, WM; Ip, JD; Cai, JP; Cheng, VCC; Chen, HL; Hui, CKM; Yuen, KY				Chan, Jasper Fuk-Woo; Yuan, Shuofeng; Kok, Kin-Hang; To, Kelvin Kai-Wang; Chu, Hin; Yang, Jin; Xing, Fanfan; Liu, Jieling; Yip, Cyril Chik-Yan; Poon, Rosana Wing-Shan; Tsoi, Hoi-Wah; Lo, Simon Kam-Fai; Chan, Kwok-Hung; Poon, Vincent Kwok-Man; Chan, Wan-Mui; Ip, Jonathan Daniel; Cai, Jian-Piao; Cheng, Vincent Chi-Chung; Chen, Honglin; Hui, Christopher Kim-Ming; Yuen, Kwok-Yung			A familial cluster of pneumonia associated with the 2019 novel coronavirus indicating person-to-person transmission: a study of a family cluster	LANCET			English	Article							RESPIRATORY SYNDROME CORONAVIRUS; VIRAL LOAD; COMMUNITY OUTBREAK; SYNDROME SARS; BETACORONAVIRUS; INVOLVEMENT; DISCOVERY; SUPPORTS; SAMPLES; VIRUS	Background An ongoing outbreak of pneumonia associated with a novel coronavirus was reported in Wuhan city, Hubei province, China. Affected patients were geographically linked with a local wet market as a potential source. No data on person-to-person or nosocomial transmission have been published to date. Methods In this study, we report the epidemiological, clinical, laboratory, radiological, and microbiological findings of five patients in a family cluster who presented with unexplained pneumonia after returning to Shenzhen, Guangdong province, China, after a visit to Wuhan, and an additional family member who did not travel to Wuhan. Phylogenetic analysis of genetic sequences from these patients were done. Findings From Jan 10, 2020, we enrolled a family of six patients who travelled to Wuhan from Shenzhen between Dec 29, 2019 and Jan 4, 2020. Of six family members who travelled to Wuhan, five were identified as infected with the novel coronavirus. Additionally, one family member, who did not travel to Wuhan, became infected with the virus after several days of contact with four of the family members. None of the family members had contacts with Wuhan markets or animals, although two had visited a Wuhan hospital. Five family members (aged 36-66 years) presented with fever, upper or lower respiratory tract symptoms, or diarrhoea, or a combination of these 3-6 days after exposure. They presented to our hospital (The University of Hong Kong-Shenzhen Hospital, Shenzhen) 6-10 days after symptom onset. They and one asymptomatic child (aged 10 years) had radiological ground-glass lung opacities. Older patients (aged >60 years) had more systemic symptoms, extensive radiological ground-glass lung changes, lymphopenia, thrombocytopenia, and increased C-reactive protein and lactate dehydrogenase levels. The nasopharyngeal or throat swabs of these six patients were negative for known respiratory microbes by point-of-care multiplex RT-PCR, but five patients (four adults and the child) were RT-PCR positive for genes encoding the internal RNA-dependent RNA polymerase and surface Spike protein of this novel coronavirus, which were confirmed by Sanger sequencing. Phylogenetic analysis of these five patients' RT-PCR amplicons and two full genomes by next-generation sequencing showed that this is a novel coronavirus, which is closest to the bat severe acute respiatory syndrome (SARS)-related coronaviruses found in Chinese horseshoe bats. Interpretation Our findings are consistent with person-to-person transmission of this novel coronavirus in hospital and family settings, and the reports of infected travellers in other geographical regions. Copyright (C) 2020 Elsevier Ltd. All rights reserved.	[Chan, Jasper Fuk-Woo; Yuan, Shuofeng; Kok, Kin-Hang; To, Kelvin Kai-Wang; Chu, Hin; Yip, Cyril Chik-Yan; Poon, Rosana Wing-Shan; Tsoi, Hoi-Wah; Chan, Kwok-Hung; Poon, Vincent Kwok-Man; Chan, Wan-Mui; Cai, Jian-Piao; Cheng, Vincent Chi-Chung; Chen, Honglin] Univ Hong Kong, Li Ka Shing Fac Med, Dept Microbiol,Pokfulam, State Key Lab Emerging Infect Dis,Carol Yu Ctr In, Hong Kong, Peoples R China; [Chan, Jasper Fuk-Woo; To, Kelvin Kai-Wang; Yang, Jin; Xing, Fanfan; Liu, Jieling; Lo, Simon Kam-Fai; Chen, Honglin; Yuen, Kwok-Yung] Univ Hong Kong, Shenzhen Hosp, Dept Clin Microbiol & Infect Control, Shenzhen 518009, Guangdong, Peoples R China; [Hui, Christopher Kim-Ming] Univ Hong Kong, Shenzhen Hosp, Dept Med, Shenzhen, Guangdong, Peoples R China	Yuen, KY (reprint author), Univ Hong Kong, Shenzhen Hosp, Dept Clin Microbiol & Infect Control, Shenzhen 518009, Guangdong, Peoples R China.	kyyuen@hku.hk			Shaw Foundation Hong Kong; Michael Seak-Kan Tong; Respiratory Viral Research Foundation; Hui Ming, Hui Hoy and Chow Sin Lan Charity Fund Limited; Hong Kong Hainan Commercial Association South China Microbiology Research Fund; Sanming Project of Medicine in Shenzhen, China [SZSM201911014, SZSM201612096]; High Level-Hospital Program, Health Commission of Guangdong Province, China	This study was partly supported by the Shaw Foundation Hong Kong; Michael Seak-Kan Tong; Respiratory Viral Research Foundation; Hui Ming, Hui Hoy and Chow Sin Lan Charity Fund Limited; Marina Man-Wai Lee; the Hong Kong Hainan Commercial Association South China Microbiology Research Fund; Sanming Project of Medicine in Shenzhen, China (SZSM201911014 and SZSM201612096); and the High Level-Hospital Program, Health Commission of Guangdong Province, China.	Centre for Health Protection of the Hong Kong Special Administrative Region Government, 2020, CHP PROV FURTH INF C; Centre for Health Protection of the Hong Kong Special Administrative Region Government, 2019, CHP CLOS MON CLUST P; Chan JFW, 2015, CLIN MICROBIOL REV, V28, P465, DOI 10.1128/CMR.00102-14; Chan JFW, 2016, EBIOMEDICINE, V14, P112, DOI 10.1016/j.ebiom.2016.11.017; Chan KH, 2017, ADV VIROL, DOI 10.1155/2017/1324276; Che XY, 2006, CLIN INFECT DIS, V43, pE1, DOI 10.1086/504943; Che XY, 2005, J INFECT DIS, V191, P2033, DOI 10.1086/430355; Cheng VCC, 2004, CLIN INFECT DIS, V38, P467, DOI 10.1086/382681; Cheng VCC, 2007, CLIN MICROBIOL REV, V20, P660, DOI 10.1128/CMR.00023-07; Cohen J., 2020, CHINESE RES REVEAL D; Hu D, 2018, EMERG MICROBES INFEC, V7, DOI 10.1038/s41426-018-0155-5; Hung IFN, 2004, EMERG INFECT DIS, V10, P1550, DOI 10.3201/eid1009.040058; Juan D., 2020, CHINADAILY; Lau SKP, 2005, P NATL ACAD SCI USA, V102, P14040, DOI 10.1073/pnas.0506735102; Lau SKP, 2015, J VIROL, V89, P10532, DOI 10.1128/JVI.01048-15; Lau SKP, 2015, J VIROL, V89, P3076, DOI 10.1128/JVI.02420-14; Leung WK, 2003, GASTROENTEROLOGY, V125, P1011, DOI [10.1016/j.gastro.2003.08.001, 10.1053/S0016-5085(03)01215-0]; Lewandowski K, 2020, J CLIN MICROBIOL, V58, DOI 10.1128/JCM.00963-19; Li WD, 2005, SCIENCE, V310, P676, DOI 10.1126/science.1118391; Memish ZA, 2014, J INFECT DIS, V210, P1590, DOI 10.1093/infdis/jiu292; Peiris JSM, 2003, LANCET, V361, P1319, DOI 10.1016/S0140-6736(03)13077-2; Peiris JSM, 2003, LANCET, V361, P1767, DOI 10.1016/S0140-6736(03)13412-5; To KKW, 2019, CLIN MICROBIOL INFEC, V25, P372, DOI 10.1016/j.cmi.2018.06.009; To KKW, 2017, J GEN VIROL, V98, P1004, DOI 10.1099/jgv.0.000766; To KKW, 2010, J MED VIROL, V82, P1, DOI 10.1002/jmv.21664; Tse H, 2012, PLOS ONE, V7, DOI 10.1371/journal.pone.0043986; Woo PCY, 2014, VIROLOGY, V471, P117, DOI 10.1016/j.virol.2014.09.020; Woo PCY, 2012, J VIROL, V86, P3995, DOI 10.1128/JVI.06540-11; Woo PCY, 2005, J VIROL, V79, P884, DOI 10.1128/JVI.79.2.884-895.2005; Zhou J, 2017, SCI ADV, V3, DOI 10.1126/sciadv.aao4966	30	71	70	180	180	ELSEVIER SCIENCE INC	NEW YORK	STE 800, 230 PARK AVE, NEW YORK, NY 10169 USA	0140-6736	1474-547X		LANCET	Lancet	FEB 15	2020	395	10223					514	523		10.1016/S0140-6736(20)30154-9			10	Medicine, General & Internal	General & Internal Medicine	KN1CG	WOS:000514576900034	31986261	Bronze			2020-04-01	
J	Jiang, SB; Xia, S; Ying, TL; Lu, L				Jiang, Shibo; Xia, Shuai; Ying, Tianlei; Lu, Lu			A novel coronavirus (2019-nCoV) causing pneumonia-associated respiratory syndrome	CELLULAR & MOLECULAR IMMUNOLOGY			English	Editorial Material; Early Access									[Jiang, Shibo; Xia, Shuai; Ying, Tianlei; Lu, Lu] Fudan Univ, Sch Basic Med Sci, 131 Dong An Rd, Shanghai 200032, Peoples R China	Jiang, SB (reprint author), Fudan Univ, Sch Basic Med Sci, 131 Dong An Rd, Shanghai 200032, Peoples R China.	shibojiang@fudan.edu.cn	Ribeiro, Nuno/AAH-2299-2020	Ying, Tianlei/0000-0002-9597-2843			Huang CL, 2020, LANCET, V395, P497, DOI 10.1016/S0140-6736(20)30183-5; Jiang SB, 2020, EMERG MICROBES INFEC, V9, P275, DOI 10.1080/22221751.2020.1723441; Li Qun, 2020, N Engl J Med, V382, P1199, DOI 10.1056/NEJMoa2001316	3	1	1	165	165	NATURE PUBLISHING GROUP	LONDON	MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND	1672-7681	2042-0226		CELL MOL IMMUNOL	Cell. Mol. Immunol.												10.1038/s41423-020-0372-4		FEB 2020	1	Immunology	Immunology	KI3YK	WOS:000511284100003	32024976	Bronze			2020-04-01	
J	[Anonymous]				[Anonymous]			COVID-19: fighting panic with information	LANCET			English	Editorial Material												Ribeiro, Nuno/AAH-2299-2020					0	0	0	162	162	ELSEVIER SCIENCE INC	NEW YORK	STE 800, 230 PARK AVE, NEW YORK, NY 10169 USA	0140-6736	1474-547X		LANCET	Lancet	FEB 22	2020	395	10224					537	537					1	Medicine, General & Internal	General & Internal Medicine	KN5AJ	WOS:000514849400006	32087777	Bronze			2020-04-01	
J	Chen, NS; Zhou, M; Dong, X; Qu, JM; Gong, FY; Han, Y; Qiu, Y; Wang, JL; Liu, Y; Wei, Y; Xia, JA; Yu, T; Zhang, XX; Zhang, L				Chen, Nanshan; Zhou, Min; Dong, Xuan; Qu, Jieming; Gong, Fengyun; Han, Yang; Qiu, Yang; Wang, Jingli; Liu, Ying; Wei, Yuan; Xia, Jia'an; Yu, Ting; Zhang, Xinxin; Zhang, Li			Epidemiological and clinical characteristics of 99 cases of 2019 novel coronavirus pneumonia in Wuhan, China: a descriptive study	LANCET			English	Article							RESPIRATORY SYNDROME CORONAVIRUS; MERS; OUTBREAK; SARS; COV	Background In December, 2019, a pneumonia associated with the 2019 novel coronavirus (2019-nCoV) emerged in Wuhan, China. We aimed to further clarify the epidemiological and clinical characteristics of 2019-nCoV pneumonia. Methods In this retrospective, single-centre study, we included all confirmed cases of 2019-nCoV in Wuhan Jinyintan Hospital from Jan 1 to Jan 20, 2020. Cases were confirmed by real-time RT-PCR and were analysed for epidemiological, demographic, clinical, and radiological features and laboratory data. Outcomes were followed up until Jan 25, 2020. Findings Of the 99 patients with 2019-nCoV pneumonia, 49 (49%) had a history of exposure to the Huanan seafood market. The average age of the patients was 55.5 years (SD 13.1), including 67 men and 32 women. 2019-nCoV was detected in all patients by real-time RT-PCR. 50 (51%) patients had chronic diseases. Patients had clinical manifestations of fever (82 [83%] patients), cough (81 [82%] patients), shortness of breath (31 [31%] patients), muscle ache (11 [11%] patients), confusion (nine [9%] patients), headache (eight [8%] patients), sore throat (five [5%] patients), rhinorrhoea (four [4%] patients), chest pain (two [2%] patients), diarrhoea (two [2%] patients), and nausea and vomiting (one [1%] patient). According to imaging examination, 74 (75%) patients showed bilateral pneumonia, 14 (14%) patients showed multiple mottling and ground-glass opacity, and one (1%) patient had pneu-mothorax. 17 (17%) patients developed acute respiratory distress syndrome and, among them, 11 (11%) patients worsened in a short period of time and died of multiple organ failure. Interpretation The 2019-nCoV infection was of clustering onset, is more likely to affect older males with comorbidities, and can result in severe and even fatal respiratory diseases such as acute respiratory distress syndrome. In general, characteristics of patients who died were in line with the MuLBSTA score, an early warning model for predicting mortality in viral pneumonia. Further investigation is needed to explore the applicability of the MuLBSTA score in predicting the risk of mortality in 2019-nCoV infection. Copyright (C) 2020 Elsevier Ltd. All rights reserved.	[Chen, Nanshan; Dong, Xuan; Wei, Yuan; Xia, Jia'an; Yu, Ting; Zhang, Li] Wuhan Jinyintan Hosp, TB & Resp Dept, Wuhan 430023, Peoples R China; [Gong, Fengyun; Wang, Jingli] Wuhan Jinyintan Hosp, Infect Dis Dept, Wuhan, Peoples R China; [Han, Yang] Wuhan Jinyintan Hosp, Sci & Educ Dept, Wuhan, Peoples R China; [Liu, Ying] Wuhan Jinyintan Hosp, Off Drug Clin Trial Inst, Wuhan, Peoples R China; [Zhou, Min; Qu, Jieming] Shanghai Jiao Tong Univ, Sch Med, Dept Resp & Crit Care Med, Ruijin Hosp, Shanghai, Peoples R China; [Zhou, Min; Qu, Jieming] Shanghai Jiao Tong Univ, Sch Med, Inst Resp Dis, Shanghai, Peoples R China; [Zhang, Xinxin] Shanghai Jiao Tong Univ, Sch Med, Res Lab Clin Virol, Ruijin Hosp, Shanghai 200025, Peoples R China; [Zhang, Xinxin] Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp North, Shanghai 200025, Peoples R China; [Zhang, Xinxin] Shanghai Jiao Tong Univ, Sch Med, Clin Res Ctr, Ruijin Hosp North, Shanghai, Peoples R China; [Han, Yang; Qiu, Yang] Chinese Acad Sci, Wuhan Inst Virol, Ctr Biosafety Mega Sci, State Key Lab Virol, Wuhan, Peoples R China	Zhang, L (reprint author), Wuhan Jinyintan Hosp, TB & Resp Dept, Wuhan 430023, Peoples R China.; Zhang, XX (reprint author), Shanghai Jiao Tong Univ, Sch Med, Res Lab Clin Virol, Ruijin Hosp, Shanghai 200025, Peoples R China.; Zhang, XX (reprint author), Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp North, Shanghai 200025, Peoples R China.	zhangx@shsmu.edu.cn; zhangli080806@163.com	Ribeiro, Nuno/AAH-2299-2020		National Key R&D Program of China [2017YFC1309700]	This study was funded by the National Key R&D Program of China (number 2017YFC1309700). We thank all patients involved in the study.	Azhar EI, 2014, NEW ENGL J MED, V370, P2499, DOI 10.1056/NEJMoa1401505; Badawi A, 2016, INT J INFECT DIS, V49, P129, DOI 10.1016/j.ijid.2016.06.015; Channappanavar R, 2017, J IMMUNOL, V198, P4046, DOI 10.4049/jimmunol.1601896; Cui J, 2019, NAT REV MICROBIOL, V17, P181, DOI 10.1038/s41579-018-0118-9; Drosten C, 2003, NEW ENGL J MED, V348, P1967, DOI 10.1056/NEJMoa030747; Dryden M, 2015, CLIN MICROBIOL INFEC, V21, pS27, DOI 10.1016/j.cmi.2015.03.024; Gao HN, 2013, NEW ENGL J MED, V368, P2277, DOI 10.1056/NEJMoa1305584; Guo LX, 2019, FRONT MICROBIOL, V10, DOI 10.3389/fmicb.2019.02752; Haagmans BL, 2014, LANCET INFECT DIS, V14, P140, DOI 10.1016/S1473-3099(13)70690-X; Hu B, 2017, PLOS PATHOG, V13, DOI 10.1371/journal.ppat.1006698; Huang CL, 2020, LANCET, V395, P497, DOI 10.1016/S0140-6736(20)30183-5; Hui DS, 2020, INT J INFECT DIS, V91, P264, DOI 10.1016/j.ijid.2020.01.009; Jaillon S, 2019, CLIN REV ALLERG IMMU, V56, P308, DOI 10.1007/s12016-017-8648-x; Liu WJ, 2017, ANTIVIR RES, V137, P82, DOI 10.1016/j.antiviral.2016.11.006; Lu HZ, 2020, J MED VIROL, V92, P401, DOI 10.1002/jmv.25678; Song HD, 2005, P NATL ACAD SCI USA, V102, P2430, DOI 10.1073/pnas.0409608102; Song ZQ, 2019, VIRUSES-BASEL, V11, DOI 10.3390/v11010059; Tao Y, 2017, J VIROL, V91, DOI 10.1128/JVI.01953-16; Wang XF, 2014, CLIN RESPIR J, V8, P410, DOI 10.1111/crj.12087; World Health Organization, 2020, CLIN MAN SEV AC RESP; Xu XT, 2020, SCI CHINA LIFE SCI, V63, P457, DOI 10.1007/s11427-020-1637-5; Yin YD, 2018, RESPIROLOGY, V23, P130, DOI 10.1111/resp.13196; Zaki AM, 2012, NEW ENGL J MED, V367, P1814, DOI 10.1056/NEJMoa1211721; Zhou P, 2018, NATURE, V556, P255, DOI 10.1038/s41586-018-0010-9	24	54	54	158	158	ELSEVIER SCIENCE INC	NEW YORK	STE 800, 230 PARK AVE, NEW YORK, NY 10169 USA	0140-6736	1474-547X		LANCET	Lancet	FEB 15	2020	395	10223					507	513		10.1016/S0140-6736(20)30211-7			7	Medicine, General & Internal	General & Internal Medicine	KN1CG	WOS:000514576900033	32007143	Bronze			2020-04-01	
J	Zhou, P; Yang, XL; Wang, XG; Hu, B; Zhang, L; Zhang, W; Si, HR; Zhu, Y; Li, B; Huang, CL; Chen, HD; Chen, J; Luo, Y; Guo, H; Jiang, RD; Liu, MQ; Chen, Y; Shen, XR; Wang, X; Zheng, XS; Zhao, K; Chen, QJ; Deng, F; Liu, LL; Yan, B; Zhan, FX; Wang, YY; Xiao, GF; Shi, ZL				Zhou, Peng; Yang, Xing-Lou; Wang, Xian-Guang; Hu, Ben; Zhang, Lei; Zhang, Wei; Si, Hao-Rui; Zhu, Yan; Li, Bei; Huang, Chao-Lin; Chen, Hui-Dong; Chen, Jing; Luo, Yun; Guo, Hua; Jiang, Ren-Di; Liu, Mei-Qin; Chen, Ying; Shen, Xu-Rui; Wang, Xi; Zheng, Xiao-Shuang; Zhao, Kai; Chen, Quan-Jiao; Deng, Fei; Liu, Lin-Lin; Yan, Bing; Zhan, Fa-Xian; Wang, Yan-Yi; Xiao, Geng-Fu; Shi, Zheng-Li			A pneumonia outbreak associated with a new coronavirus of probable bat origin	NATURE			English	Article							SARS; IDENTIFICATION	Since the outbreak of severe acute respiratory syndrome (SARS) 18 years ago, a large number of SARS-related coronaviruses (SARSr-CoVs) have been discovered in their natural reservoir host, bats(1-4). Previous studies have shown that some bat SARSr-CoVs have the potential to infect humans(5-7). Here we report the identification and characterization of a new coronavirus (2019-nCoV), which caused an epidemic of acute respiratory syndrome in humans in Wuhan, China. The epidemic, which started on 12 December 2019, had caused 2,794 laboratory-confirmed infections including 80 deaths by 26 January 2020. Full-length genome sequences were obtained from five patients at an early stage of the outbreak. The sequences are almost identical and share 79.6% sequence identity to SARS-CoV. Furthermore, we show that 2019-nCoV is 96% identical at the whole-genome level to a bat coronavirus. Pairwise protein sequence analysis of seven conserved non-structural proteins domains show that this virus belongs to the species of SARSr-CoV. In addition, 2019-nCoV virus isolated from the bronchoalveolar lavage fluid of a critically ill patient could be neutralized by sera from several patients. Notably, we confirmed that 2019-nCoV uses the same cell entry receptor-angiotensin converting enzyme II (ACE2)-as SARS-CoV.	[Zhou, Peng; Yang, Xing-Lou; Hu, Ben; Zhang, Lei; Zhang, Wei; Si, Hao-Rui; Zhu, Yan; Li, Bei; Chen, Jing; Luo, Yun; Guo, Hua; Jiang, Ren-Di; Liu, Mei-Qin; Chen, Ying; Shen, Xu-Rui; Wang, Xi; Zheng, Xiao-Shuang; Zhao, Kai; Chen, Quan-Jiao; Deng, Fei; Yan, Bing; Wang, Yan-Yi; Xiao, Geng-Fu; Shi, Zheng-Li] Chinese Acad Sci, Wuhan Inst Virol, Ctr Biosafety Mega Sci, CAS Key Lab Special Pathogens, Wuhan, Peoples R China; [Wang, Xian-Guang; Huang, Chao-Lin; Chen, Hui-Dong] Wuhan Jin Yin Tan Hosp, Wuhan, Peoples R China; [Si, Hao-Rui; Chen, Jing; Luo, Yun; Guo, Hua; Jiang, Ren-Di; Liu, Mei-Qin; Chen, Ying; Shen, Xu-Rui; Wang, Xi; Zheng, Xiao-Shuang; Zhao, Kai] Univ Chinese Acad Sci, Beijing, Peoples R China; [Liu, Lin-Lin; Zhan, Fa-Xian] Hubei Prov Ctr Dis Control & Prevent, Wuhan, Peoples R China	Shi, ZL (reprint author), Chinese Acad Sci, Wuhan Inst Virol, Ctr Biosafety Mega Sci, CAS Key Lab Special Pathogens, Wuhan, Peoples R China.	zlshi@wh.iov.cn			Strategic Priority Research Program of the Chinese Academy of Sciences (CAS) [XDB29010101, XDB29010104]; China Natural Science Foundation for excellent scholars [81822028, 31770175, 31800142]; Mega-Project for Infectious Disease from Minister of Science and Technology of the People's Republic of China [2018ZX10305409-004-001]; Youth innovation promotion association of CAS [2019328]	We thank P. Zhang and A. Du from the WIV core facility centre for their help with producing transmission electron microscopy micrographs; H.-Z. Liu and P. Yu from WIV for bioinformatics analysis. This work was jointly supported by the Strategic Priority Research Program of the Chinese Academy of Sciences (CAS) (XDB29010101 to Z.-L.S. and XDB29010104 to P.Z.), China Natural Science Foundation for excellent scholars (81822028 to P.Z., 31770175 to Z.-L.S. and 31800142 to B.H.), Mega-Project for Infectious Disease from Minister of Science and Technology of the People's Republic of China (2018ZX10305409-004-001 to P.Z.), Youth innovation promotion association of CAS (2019328 to X.-L.Y.).	Cui J, 2019, NAT REV MICROBIOL, V17, P181, DOI 10.1038/s41579-018-0118-9; Drosten C, 2003, NEW ENGL J MED, V348, P1967, DOI 10.1056/NEJMoa030747; Fan Y, 2019, VIRUSES-BASEL, V11, DOI 10.3390/v11030210; Ge XY, 2013, NATURE, V503, P535, DOI 10.1038/nature12711; Gorbalenya A. E, 2020, SEVERE ACUTE RESP SY, DOI DOI 10.1101/2020.02.07.937862V1; Hu B, 2017, PLOS PATHOG, V13, DOI 10.1371/journal.ppat.1006698; Li WD, 2005, SCIENCE, V310, P676, DOI 10.1126/science.1118391; Li WH, 2003, NATURE, V426, P450, DOI 10.1038/nature02145; Menachery VD, 2016, P NATL ACAD SCI USA, V113, P3048, DOI 10.1073/pnas.1517719113; Menachery VD, 2015, NAT MED, V21, P1508, DOI 10.1038/nm.3985; Poon LLM, 2005, J VIROL, V79, P2001, DOI 10.1128/JVI.79.4.2001-2009.2005; Wang N, 2018, VIROL SIN, V33, P104, DOI 10.1007/s12250-018-0012-7; WHO, 2020, WHO DIR GEN REM MED; Wuhan Municipal Health Commission, 2020, PRESS STAT REL NOV C; Yang L, 2013, EMERG INFECT DIS, V19, P989, DOI 10.3201/eid1906.121648; Zaki AM, 2012, NEW ENGL J MED, V367, P1814, DOI 10.1056/NEJMoa1211721	16	59	59	157	157	NATURE PUBLISHING GROUP	LONDON	MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND	0028-0836	1476-4687		NATURE	Nature	MAR	2020	579	7798					270	+		10.1038/s41586-020-2012-7			16	Multidisciplinary Sciences	Science & Technology - Other Topics	KU0CC	WOS:000519378900030	32015507	Other Gold			2020-04-01	
J	Zhou, P; Yang, XL; Wang, XG; Hu, B; Zhang, L; Zhang, W; Si, HR; Zhu, Y; Li, B; Huang, CL; Chen, HD; Chen, J; Luo, Y; Guo, H; Jiang, RD; Liu, MQ; Chen, Y; Shen, XR; Wang, X; Zheng, XS; Zhao, K; Chen, QJ; Deng, F; Liu, LL; Yan, B; Zhan, FX; Wang, YY; Xiao, GF; Shi, ZL				Zhou, Peng; Yang, Xing-Lou; Wang, Xian-Guang; Hu, Ben; Zhang, Lei; Zhang, Wei; Si, Hao-Rui; Zhu, Yan; Li, Bei; Huang, Chao-Lin; Chen, Hui-Dong; Chen, Jing; Luo, Yun; Guo, Hua; Jiang, Ren-Di; Liu, Mei-Qin; Chen, Ying; Shen, Xu-Rui; Wang, Xi; Zheng, Xiao-Shuang; Zhao, Kai; Chen, Quan-Jiao; Deng, Fei; Liu, Lin-Lin; Yan, Bing; Zhan, Fa-Xian; Wang, Yan-Yi; Xiao, Geng-Fu; Shi, Zheng-Li			A pneumonia outbreak associated with a new coronavirus of probable bat origin	NATURE			English	Article; Early Access							SARS; IDENTIFICATION	Since the outbreak of severe acute respiratory syndrome (SARS) 18 years ago, a large number of SARS-related coronaviruses (SARSr-CoVs) have been discovered in their natural reservoir host, bats(1-4). Previous studies have shown that some bat SARSr-CoVs have the potential to infect humans(5-7). Here we report the identification and characterization of a new coronavirus (2019-nCoV), which caused an epidemic of acute respiratory syndrome in humans in Wuhan, China. The epidemic, which started on 12 December 2019, had caused 2,794 laboratory-confirmed infections including 80 deaths by 26 January 2020. Full-length genome sequences were obtained from five patients at an early stage of the outbreak. The sequences are almost identical and share 79.6% sequence identity to SARS-CoV. Furthermore, we show that 2019-nCoV is 96% identical at the whole-genome level to a bat coronavirus. Pairwise protein sequence analysis of seven conserved non-structural proteins domains show that this virus belongs to the species of SARSr-CoV. In addition, 2019-nCoV virus isolated from the bronchoalveolar lavage fluid of a critically ill patient could be neutralized by sera from several patients. Notably, we confirmed that 2019-nCoV uses the same cell entry receptor-angiotensin converting enzyme II (ACE2)-as SARS-CoV. Characterization of full-length genome sequences from patients infected with a new coronavirus (2019-nCoV) shows that the sequences are nearly identical and indicates that the virus is related to a bat coronavirus.	[Zhou, Peng; Yang, Xing-Lou; Hu, Ben; Zhang, Lei; Zhang, Wei; Si, Hao-Rui; Zhu, Yan; Li, Bei; Chen, Jing; Luo, Yun; Guo, Hua; Jiang, Ren-Di; Liu, Mei-Qin; Chen, Ying; Shen, Xu-Rui; Wang, Xi; Zheng, Xiao-Shuang; Zhao, Kai; Chen, Quan-Jiao; Deng, Fei; Yan, Bing; Wang, Yan-Yi; Xiao, Geng-Fu; Shi, Zheng-Li] Chinese Acad Sci, Ctr Biosafety Mega Sci, Wuhan Inst Virol, CAS Key Lab Special Pathogens, Wuhan, Peoples R China; [Wang, Xian-Guang; Huang, Chao-Lin; Chen, Hui-Dong] Wuhan Jin Yin Tan Hosp, Wuhan, Peoples R China; [Si, Hao-Rui; Chen, Jing; Luo, Yun; Guo, Hua; Jiang, Ren-Di; Liu, Mei-Qin; Chen, Ying; Shen, Xu-Rui; Wang, Xi; Zheng, Xiao-Shuang; Zhao, Kai] Univ Chinese Acad Sci, Beijing, Peoples R China; [Liu, Lin-Lin; Zhan, Fa-Xian] Hubei Prov Ctr Dis Control & Prevent, Wuhan, Peoples R China	Shi, ZL (reprint author), Chinese Acad Sci, Ctr Biosafety Mega Sci, Wuhan Inst Virol, CAS Key Lab Special Pathogens, Wuhan, Peoples R China.	zlshi@wh.iov.cn			Strategic Priority Research Program of the Chinese Academy of Sciences (CAS) [XDB29010101, XDB29010104]; China Natural Science FoundationNational Natural Science Foundation of China [81822028, 31770175, 31800142]; Mega-Project for Infectious Disease [2018ZX10305409-004-001]; Youth innovation promotion association of CAS [2019328]	We thank P. Zhang and A. Du from the WIV core facility centre for their help with producing transmission electron microscopy micrographs; H.-Z. Liu and P. Yu from WIV for bioinformatics analysis. This work was jointly supported by the Strategic Priority Research Program of the Chinese Academy of Sciences (CAS) (XDB29010101 to Z.-L.S. and XDB29010104 to P.Z.), China Natural Science Foundation for excellent scholars (81822028 to P.Z., 31770175 to Z.-L.S. and 31800142 to B.H.), Mega-Project for Infectious Disease from Minister of Science and Technology of the People's Republic of China (2018ZX10305409-004-001 to P.Z.), Youth innovation promotion association of CAS (2019328 to X.-L.Y.).	Cui J, 2019, NAT REV MICROBIOL, V17, P181, DOI 10.1038/s41579-018-0118-9; Drosten C, 2003, NEW ENGL J MED, V348, P1967, DOI 10.1056/NEJMoa030747; Fan Y, 2019, VIRUSES-BASEL, V11, DOI 10.3390/v11030210; Ge XY, 2013, NATURE, V503, P535, DOI 10.1038/nature12711; Gorbalenya A. E, 2020, SEVERE ACUTE RESP SY, DOI DOI 10.1101/2020.02.07.937862V1; Hu B, 2017, PLOS PATHOG, V13, DOI 10.1371/journal.ppat.1006698; Li WD, 2005, SCIENCE, V310, P676, DOI 10.1126/science.1118391; Li WH, 2003, NATURE, V426, P450, DOI 10.1038/nature02145; Menachery VD, 2016, P NATL ACAD SCI USA, V113, P3048, DOI 10.1073/pnas.1517719113; Menachery VD, 2015, NAT MED, V21, P1508, DOI 10.1038/nm.3985; Poon LLM, 2005, J VIROL, V79, P2001, DOI 10.1128/JVI.79.4.2001-2009.2005; Wang N, 2018, VIROL SIN, V33, P104, DOI 10.1007/s12250-018-0012-7; WHO, 2020, WHO DIR GEN REM MED; Wuhan Municipal Health Commission, 2020, PRESS STAT REL NOV C; Yang L, 2013, EMERG INFECT DIS, V19, P989, DOI 10.3201/eid1906.121648; Zaki AM, 2012, NEW ENGL J MED, V367, P1814, DOI 10.1056/NEJMoa1211721	16	59	59	157	157	NATURE PUBLISHING GROUP	LONDON	MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND	0028-0836	1476-4687		NATURE	Nature												10.1038/s41586-020-2012-7		FEB 2020	20	Multidisciplinary Sciences	Science & Technology - Other Topics	KS1VQ	WOS:000518098100001	32015507	Other Gold			2020-04-01	
J	Corman, VM; Landt, O; Kaiser, M; Molenkamp, R; Meijer, A; Chu, DKW; Bleicker, T; Brunink, S; Schneider, J; Schmidt, ML; Mulders, DGJC; Haagmans, BL; van der Veer, B; van den Brink, S; Wijsman, L; Goderski, G; Romette, JL; Ellis, J; Zambon, M; Peiris, M; Goossens, H; Reusken, C; Koopmans, MPG; Drosten, C				Corman, Victor M.; Landt, Olfert; Kaiser, Marco; Molenkamp, Richard; Meijer, Adam; Chu, Daniel K. W.; Bleicker, Tobias; Bruenink, Sebastian; Schneider, Julia; Schmidt, Marie Luisa; Mulders, Daphne G. J. C.; Haagmans, Bart L.; van der Veer, Bas; van den Brink, Sharon; Wijsman, Lisa; Goderski, Gabriel; Romette, Jean-Louis; Ellis, Joanna; Zambon, Maria; Peiris, Malik; Goossens, Herman; Reusken, Chantal; Koopmans, Marion P. G.; Drosten, Christian			Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR	EUROSURVEILLANCE			English	Article							CLASSIFICATION	Background: The ongoing outbreak of the recently emerged novel coronavirus (2019-nCoV) poses a challenge for public health laboratories as virus isolates are unavailable while there is growing evidence that the outbreak is more widespread than initially thought, and international spread through travellers does already occur. Aim: We aimed to develop and deploy robust diagnostic methodology for use in public health laboratory settings without having virus material available. Methods: Here we present a validated diagnostic workflow for 2019-nCoV, its design relying on close genetic relatedness of 2019-nCoV with SARS coronavirus, making use of synthetic nucleic acid technology. Results: The workflow reliably detects 2019-nCoV, and further discriminates 2019-nCoV from SARS-CoV. Through coordination between academic and public laboratories, we confirmed assay exclusivity based on 297 original clinical specimens containing a full spectrum of human respiratory viruses. Control material is made available through European Virus Archive - Global ( EVAg), a European Union infrastructure project. Conclusion: The present study demonstrates the enormous response capacity achieved through coordination of academic and public laboratories in national and European research networks.	[Corman, Victor M.; Bleicker, Tobias; Bruenink, Sebastian; Schneider, Julia; Schmidt, Marie Luisa; Drosten, Christian] Charite Univ Med Berlin, Inst Virol, Berlin, Germany; [Corman, Victor M.; Bleicker, Tobias; Bruenink, Sebastian; Schneider, Julia; Schmidt, Marie Luisa; Drosten, Christian] German Ctr Infect Res DZIF, Berlin, Germany; [Landt, Olfert; Kaiser, Marco] Tib Molbiol, Berlin, Germany; [Molenkamp, Richard; Mulders, Daphne G. J. C.; Haagmans, Bart L.; Koopmans, Marion P. G.] Erasmus MC, Dept Virosci, Rotterdam, Netherlands; [Meijer, Adam; van der Veer, Bas; van den Brink, Sharon; Wijsman, Lisa; Goderski, Gabriel; Reusken, Chantal] Natl Inst Publ Hlth & Environm RIVM, Bilthoven, Netherlands; [Peiris, Malik] Univ Hong Kong, Hong Kong, Peoples R China; [Romette, Jean-Louis] Univ Aix Marseille, Marseille, France; [Ellis, Joanna; Zambon, Maria] Publ Hlth England, London, England; [Goossens, Herman] Univ Antwerp, Vaccine & Infect Dis Inst, Dept Med Microbiol, Antwerp, Belgium	Drosten, C (reprint author), Charite Univ Med Berlin, Inst Virol, Berlin, Germany.; Drosten, C (reprint author), German Ctr Infect Res DZIF, Berlin, Germany.	christian.drosten@charite.de	Ribeiro, Nuno/AAH-2299-2020; Corman, Victor Max/K-1319-2019	Corman, Victor Max/0000-0002-3605-0136; Haagmans, Bart/0000-0001-6221-2015	European Union DG Research through project Prepare [GA602525]; European Union DG Research through project Compare [GA643476]; European Union DG Research through project EVAg [GA653316]; European Union DG SANCO through EVD-LabNet; German Ministry of Research through project RAPID [01KI1723A]; German Ministry of Research through project DZIF [301-4-7-01.703]	This work was funded by European Union DG Research through projects Prepare (GA602525), Compare (GA643476), and EVAg (GA653316); by European Union DG SANCO through EVD-LabNet, as well as by the German Ministry of Research through projects RAPID (01KI1723A) and DZIF (301-4-7-01.703).	Abbott A, 2003, NATURE, V423, P114, DOI 10.1038/423114a; Alleweldt F, 2017, REV SCI TECH OIE, V36, P311, DOI 10.20506/rst.36.1.2631; Corman VM, 2013, EUROSURVEILLANCE, V18, P10; Corman VM, 2012, EUROSURVEILLANCE, V17, P2; Corman VM, 2012, EUROSURVEILLANCE, V17, P3; Corman VM, 2018, ADV VIRUS RES, V100, P163, DOI 10.1016/bs.aivir.2018.01.001; Corman VM, 2016, B WORLD HEALTH ORGAN, V94, P880, DOI 10.2471/BLT.16.175950; Cui J, 2019, NAT REV MICROBIOL, V17, P181, DOI 10.1038/s41579-018-0118-9; deGroot R.J., 2012, VIRUS TAXONOMY, P806; Domingo C, 2018, EUROSURVEILLANCE, V23, P11, DOI 10.2807/1560-7917.ES.2018.23.28.1800341; Drexler JF, 2014, ANTIVIR RES, V101, P45, DOI 10.1016/j.antiviral.2013.10.013; Drexler JF, 2010, J VIROL, V84, P11336, DOI 10.1128/JVI.00650-10; Drosten C, 2003, NEW ENGL J MED, V348, P1967, DOI 10.1056/NEJMoa030747; Gobat Nina, 2019, ERJ Open Res, V5, DOI 10.1183/23120541.00227-2018; Muth D, 2018, SCI REP-UK, V8, DOI 10.1038/s41598-018-33487-8; Panning M, 2009, EMERG INFECT DIS, V15, P469, DOI 10.3201/eid1503.081104; Pas SD, 2015, J CLIN VIROL, V69, P81, DOI 10.1016/j.jcv.2015.05.022; Peiris JSM, 2003, NEW ENGL J MED, V349, P2431, DOI 10.1056/NEJMra032498; Romette JL, 2018, ANTIVIR RES, V158, P127, DOI 10.1016/j.antiviral.2018.07.017; World Health Organization, 2020, 1 WHO; World Health Organization (WHO), 2020, COR; Zhang Y-Z, NOVEL 2019 CORONAVIR	22	16	16	155	155	EUR CENTRE DIS PREVENTION & CONTROL	STOCKHOLM	TOMTEBODAVAGEN 11A, STOCKHOLM, 171 83, SWEDEN	1560-7917			EUROSURVEILLANCE	Eurosurveillance	JAN 23	2020	25	3					23	30		10.2807/1560-7917.ES.2020.25.3.2000045			8	Infectious Diseases	Infectious Diseases	KF6OZ	WOS:000509360400005	31992387	DOAJ Gold, Green Published			2020-04-01	
J	Lu, HZ; Stratton, CW; Tang, YW				Lu, Hongzhou; Stratton, Charles W.; Tang, Yi-Wei			Outbreak of pneumonia of unknown etiology in Wuhan, China: The mystery and the miracle	JOURNAL OF MEDICAL VIROLOGY			English	Editorial Material							SARS; CORONAVIRUS; IDENTIFICATION; MERS		[Lu, Hongzhou] Fudan Univ, Shanghai Publ Hlth Clin Ctr, Shanghai 201508, Peoples R China; [Stratton, Charles W.] Vanderbilt Univ, Med Ctr, Dept Pathol Microbiol & Immunol, Nashville, TN USA; [Tang, Yi-Wei] Cepheid, Danaher Diagnost Platform, Shanghai, Peoples R China	Lu, HZ (reprint author), Fudan Univ, Shanghai Publ Hlth Clin Ctr, Shanghai 201508, Peoples R China.; Tang, YW (reprint author), Cepheid, Danaher Diagnost Platform, Shanghai, Peoples R China.	luhongzhou@fudan.edu.cn; yi-wei.tang@cepheid.com		Tang, Yi-Wei/0000-0003-4888-6771			Cavanagh D, 2005, BIRK ADV INFECT DIS, P1; de Wit E, 2016, NAT REV MICROBIOL, V14, P523, DOI 10.1038/nrmicro.2016.81; Drosten C, 2003, NEW ENGL J MED, V348, P1967, DOI 10.1056/NEJMoa030747; Fredricks DN, 1996, CLIN MICROBIOL REV, V9, P18, DOI 10.1128/CMR.9.1.18; Guan Y, 2003, SCIENCE, V302, P276, DOI 10.1126/science.1087139; Kan B, 2005, J VIROL, V79, P11892, DOI 10.1128/JVI.79.18.11892-11900.2005; Li WD, 2005, SCIENCE, V310, P676, DOI 10.1126/science.1118391; Yin YD, 2018, RESPIROLOGY, V23, P130, DOI 10.1111/resp.13196; Zumla A, 2016, NAT REV DRUG DISCOV, V15, P327, DOI 10.1038/nrd.2015.37	9	18	18	142	142	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0146-6615	1096-9071		J MED VIROL	J. Med. Virol.	APR	2020	92	4			SI		401	402		10.1002/jmv.25678		FEB 2020	2	Virology	Virology	KM6QU	WOS:000512726300001	31950516	Bronze			2020-04-01	
J	Wang, WE; Tang, JM; Wei, FQ				Wang, Weier; Tang, Jianming; Wei, Fangqiang			Updated understanding of the outbreak of 2019 novel coronavirus (2019-nCoV) in Wuhan, China	JOURNAL OF MEDICAL VIROLOGY			English	Article						coronavirus; epidemiology; infection	RESPIRATORY SYNDROME; SARS	To help health workers and the public recognize and deal with the 2019 novel coronavirus (2019-nCoV) quickly, effectively, and calmly with an updated understanding. A comprehensive search from Chinese and worldwide official websites and announcements was performed between 1 December 2019 and 9:30 am 26 January 2020 (Beijing time). A latest summary of 2019-nCoV and the current outbreak was drawn. Up to 24 pm, 25 January 2020, a total of 1975 cases of 2019-nCoV infection were confirmed in mainland China with a total of 56 deaths having occurred. The latest mortality was approximately 2.84% with a total of 2684 cases still suspected. The China National Health Commission reported the details of the first 17 deaths up to 24 pm, 22 January 2020. The deaths included 13 males and 4 females. The median age of the people who died was 75 (range 48-89) years. Fever (64.7%) and cough (52.9%) were the most common first symptoms among those who died. The median number of days from the occurence of the first symptom to death was 14.0 (range 6-41) days, and it tended to be shorter among people aged 70 years or more (11.5 [range 6-19] days) than those aged less than 70 years (20 [range 10-41] days; P = .033). The 2019-nCoV infection is spreading and its incidence is increasing nationwide. The first deaths occurred mostly in elderly people, among whom the disease might progress faster. The public should still be cautious in dealing with the virus and pay more attention to protecting the elderly people from the virus.	[Wang, Weier; Wei, Fangqiang] Hangzhou Med Coll, Zhejiang Prov Peoples Hosp, Dept Hepatobiliary & Pancreat Surg, Hangzhou 310014, Zhejiang, Peoples R China; [Wang, Weier] Zhejiang Chinese Med Univ, Clin Med Coll 2, Hangzhou, Zhejiang, Peoples R China; [Tang, Jianming] Hangzhou Med Coll, Zhejiang Prov Peoples Hosp, Dept Radiat Oncol, Hangzhou 310014, Zhejiang, Peoples R China	Wei, FQ (reprint author), Hangzhou Med Coll, Zhejiang Prov Peoples Hosp, Dept Hepatobiliary & Pancreat Surg, Hangzhou 310014, Zhejiang, Peoples R China.; Tang, JM (reprint author), Hangzhou Med Coll, Zhejiang Prov Peoples Hosp, Dept Radiat Oncol, Hangzhou 310014, Zhejiang, Peoples R China.	15900792812@163.com; wdfwfq@126.com	Ribeiro, Nuno/AAH-2299-2020	Wei, Fangqiang/0000-0003-0871-2275			[Anonymous], 2020, Nature, V577, P450, DOI 10.1038/d41586-020-00153-x; [Anonymous], 2020, CHINA YOUTH DAILYS B; Centers for Disease Control and Prevention, 2020, UPD INT GUID OUTBR 2; Centers for Disease Control and Prevention, 2020, INT 2019 NOV COR 201; Chan JFW, 2020, LANCET, V395, P514, DOI 10.1016/S0140-6736(20)30154-9; Chen Y, 2020, J MED VIROL, V92, P418, DOI 10.1002/jmv.25681; China Disease Prevention and Control Center, PREV CONTR PNEUM NOV; Feng D, 2009, TROP MED INT HEALTH, V14, P28, DOI 10.1111/j.1365-3156.2008.02188.x; Huang C, 2020, LANCET, DOI [10.1016/S0140-6736(20)30183-5A, DOI 10.1016/S0140-6736(20)30183-5A]; Ji W, 2020, J MED VIROL, V92, P433, DOI 10.1002/jmv.25682; Kan B, 2005, J VIROL, V79, P11892, DOI 10.1128/JVI.79.18.11892-11900.2005; Li WD, 2005, SCIENCE, V310, P676, DOI 10.1126/science.1118391; Luo G, 2020, J MED VIROL, V92, P399, DOI 10.1002/jmv.25683; Oh MD, 2018, KOREAN J INTERN MED, V33, P233, DOI 10.3904/kjim.2018.031; WHO, 2003, AC RESP SYNDR CHIN U; WHO, 2020, M INT HLTH REG 2005; WHO, HOM CAR PAT SUSP NOV; WHO, 2020, INF PREV CONTR HLTH; *WHO, SUMM PROB SARS CAS O; World Health Organization, MIDDL E RESP SYNDR C; World Health Organization (WHO), COR; XINHUANET, 2020, XINHUANET; Xu XT, 2020, SCI CHINA LIFE SCI, V63, P457, DOI 10.1007/s11427-020-1637-5	23	7	7	136	136	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0146-6615	1096-9071		J MED VIROL	J. Med. Virol.	APR	2020	92	4			SI		441	447		10.1002/jmv.25689		FEB 2020	7	Virology	Virology	KM6QU	WOS:000512727900001	31994742	Bronze			2020-04-01	
J	Wang, ML; Cao, RY; Zhang, LK; Yang, XL; Liu, J; Xu, MY; Shi, ZL; Hu, ZH; Zhong, W; Xiao, GF				Wang, Manli; Cao, Ruiyuan; Zhang, Leike; Yang, Xinglou; Liu, Jia; Xu, Mingyue; Shi, Zhengli; Hu, Zhihong; Zhong, Wu; Xiao, Gengfu			Remdesivir and chloroquine effectively inhibit the recently emerged novel coronavirus (2019-nCoV) in vitro	CELL RESEARCH			English	Letter							VIRUS-INFECTION; EBOLA-VIRUS		[Wang, Manli; Zhang, Leike; Yang, Xinglou; Liu, Jia; Xu, Mingyue; Shi, Zhengli; Hu, Zhihong; Xiao, Gengfu] Chinese Acad Sci, Wuhan Inst Virol, Ctr Biosafety Mega Sci, State Key Lab Virol, Wuhan 430071, Peoples R China; [Cao, Ruiyuan; Zhong, Wu] Beijing Inst Pharmacol & Toxicol, Natl Engn Res Ctr Emergency Drug, Beijing 100850, Peoples R China	Hu, ZH; Xiao, GF (reprint author), Chinese Acad Sci, Wuhan Inst Virol, Ctr Biosafety Mega Sci, State Key Lab Virol, Wuhan 430071, Peoples R China.; Zhong, W (reprint author), Beijing Inst Pharmacol & Toxicol, Natl Engn Res Ctr Emergency Drug, Beijing 100850, Peoples R China.	huzh@wh.iov.cn; zhongwu@bmi.ac.cn; xiaogf@wh.iov.cn	Ribeiro, Nuno/AAH-2299-2020	Hu, Zhihong/0000-0002-1560-0928	National Science and Technology Major Projects for "Major New Drugs Innovation and Development" [2018ZX09711003]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [31621061]; Emergency Scientific Research Project for 2019-nCoV from Hubei Province	We thank Xi Wang, Yan Wu, Weijuan Shang, Huanyu Zhang, Yufeng Li, Hengrui Hu, Xiaming Jiang, Yuan Sun, from Wuhan Institute of Virology for their essential assistance with this study. We thank Prof. Fei Deng from National Virus Resource Center, and Tao Du, Jia Wu and Hao Tang from BSL-3 Laboratory of Wuhan Institute of Virology for their critical support. We thank Prof. Yanyi Wang and other colleagues of Wuhan Institute of Virology and Wuhan National Biosafety Laboratory for their excellent coordination. We thank Dr. Basil Arif for scientific editing of the manuscript. We thank the anonymous reviewers for their valuable suggestions. This work was supported in part by grants from the National Science and Technology Major Projects for "Major New Drugs Innovation and Development" (directed by Prof. Song Li) (2018ZX09711003), the National Natural Science Foundation of China (31621061), and the Emergency Scientific Research Project for 2019-nCoV from Hubei Province (to Profs. Zhengli Shi and Gengfu Xiao).	Huang CL, 2020, LANCET, V395, P497, DOI 10.1016/S0140-6736(20)30183-5; MACKENZIE AH, 1983, AM J MED, V75, P40, DOI 10.1016/0002-9343(83)91269-X; Mulangu S, 2019, NEW ENGL J MED, V381, P2293, DOI 10.1056/NEJMoa1910993; Oestereich L, 2014, ANTIVIR RES, V105, P17, DOI 10.1016/j.antiviral.2014.02.014; Savarino A, 2006, LANCET INFECT DIS, V6, P67, DOI 10.1016/S1473-3099(06)70361-9; Sheahan TP, 2017, SCI TRANSL MED, V9, DOI 10.1126/scitranslmed.aal3653; Vincent MJ, 2005, VIROL J, V2, DOI 10.1186/1743-422X-2-69; Warren TK, 2016, NATURE, V531, P381, DOI 10.1038/nature17180; Yan YW, 2013, CELL RES, V23, P300, DOI 10.1038/cr.2012.165; Zhou P, NATURE; Zumla A, 2016, NAT REV DRUG DISCOV, V15, P327, DOI 10.1038/nrd.2015.37	11	17	18	129	129	NATURE PUBLISHING GROUP	LONDON	MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND	1001-0602	1748-7838		CELL RES	Cell Res.	MAR	2020	30	3					269	271		10.1038/s41422-020-0282-0		FEB 2020	3	Cell Biology	Cell Biology	KS3GF	WOS:000511096100001	32020029	Green Published, Other Gold			2020-04-01	
J	Han, WZ; Quan, B; Guo, Y; Zhang, J; Lu, Y; Feng, G; Wu, QW; Fang, F; Cheng, L; Jiao, NL; Li, XN; Chen, Q				Han, Wenzheng; Quan, Bin; Guo, Yi; Zhang, Jun; Lu, Yong; Feng, Gang; Wu, Qiwen; Fang, Fang; Cheng, Long; Jiao, Nanlin; Li, Xiaoning; Chen, Qing			The course of clinical diagnosis and treatment of a case infected with coronavirus disease 2019	JOURNAL OF MEDICAL VIROLOGY			English	Letter						COVID-19; lopinavir and ritonavir tablets; SARS-CoV-2; severe respiratory syndrome			[Han, Wenzheng; Feng, Gang; Wu, Qiwen; Fang, Fang; Cheng, Long; Li, Xiaoning] Wanan Med Coll, Dept Clin Lab, Affiliated Hosp 1, Zheshan West Rd, Wuhu 241001, Anhui, Peoples R China; [Quan, Bin] Wanan Med Coll, Dept Infect Dis, Affiliated Hosp 1, Wuhu, Anhui, Peoples R China; [Guo, Yi] Wanan Med Coll, Dept Gynaecol & Obstet, Affiliated Hosp 1, Wuhu, Anhui, Peoples R China; [Zhang, Jun] Wanan Med Coll, Dept Clin Blood Lab, Affiliated Hosp 1, Wuhu, Anhui, Peoples R China; [Lu, Yong] Wanan Med Coll, Sch Lab Med, Dept Med Lab Sci, Wuhu, Anhui, Peoples R China; [Jiao, Nanlin] Wanan Med Coll, Dept Pathol, Affiliated Hosp 1, Wuhu, Anhui, Peoples R China; [Chen, Qing] Wanan Med Coll, Dept Nucl Med, Affiliated Hosp 1, Zheshan West Rd, Wuhu 241001, Anhui, Peoples R China	Li, XN (reprint author), Wanan Med Coll, Dept Clin Lab, Affiliated Hosp 1, Zheshan West Rd, Wuhu 241001, Anhui, Peoples R China.; Chen, Q (reprint author), Wanan Med Coll, Dept Nucl Med, Affiliated Hosp 1, Zheshan West Rd, Wuhu 241001, Anhui, Peoples R China.	lixiaoning19702006@126.com; 11418163@zju.edu.cn			Natural Science Foundation of Anhui ProvinceNatural Science Foundation of Anhui Province [1908085QH325]; Natural science research project of universities in Anhui Province [KJ2017A260]	This work was supported by the Natural Science Foundation of Anhui Province (no. 1908085QH325) and the Natural science research project of universities in Anhui Province (no. KJ2017A260). The authors thank the patient for participation in this study.	Centers for Disease Control and Prevention, 2020, INT GUID COLL HANDL; Chan JFW, 2020, LANCET, V395, P514, DOI 10.1016/S0140-6736(20)30154-9; Chen NS, 2020, LANCET, V395, P507, DOI 10.1016/S0140-6736(20)30211-7; Corman VM, 2020, EUROSURVEILLANCE, V25, P23, DOI 10.2807/1560-7917.ES.2020.25.3.2000045; Guan W, 2020, CLIN CHARACTERISTICS, DOI [10.1101/2020.02.06.20020974, DOI 10.1101/2020.02.06.20020974]; Wang Dawei, 2020, JAMA, DOI 10.1001/jama.2020.1585; Zhu N, 2020, NEW ENGL J MED, V382, P727, DOI 10.1056/NEJMoa2001017	7	0	0	121	121	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0146-6615	1096-9071		J MED VIROL	J. Med. Virol.	MAY	2020	92	5					461	463		10.1002/jmv.25711		MAR 2020	3	Virology	Virology	KU3AY	WOS:000517096300001	32073161	Bronze			2020-04-01	
J	Benvenuto, D; Giovanetti, M; Ciccozzi, A; Spoto, S; Angeletti, S; Ciccozzi, M				Benvenuto, Domenico; Giovanetti, Marta; Ciccozzi, Alessandra; Spoto, Silvia; Angeletti, Silvia; Ciccozzi, Massimo			The 2019-new coronavirus epidemic: Evidence for virus evolution	JOURNAL OF MEDICAL VIROLOGY			English	Article						coronavirus; epidemiology; macromolecular design; SARS coronavirus		There is a worldwide concern about the new coronavirus 2019-nCoV as a global public health threat. In this article, we provide a preliminary evolutionary and molecular epidemiological analysis of this new virus. A phylogenetic tree has been built using the 15 available whole genome sequences of 2019-nCoV, 12 whole genome sequences of 2019-nCoV, and 12 highly similar whole genome sequences available in gene bank (five from the severe acute respiratory syndrome, two from Middle East respiratory syndrome, and five from bat SARS-like coronavirus). Fast unconstrained Bayesian approximation analysis shows that the nucleocapsid and the spike glycoprotein have some sites under positive pressure, whereas homology modeling revealed some molecular and structural differences between the viruses. The phylogenetic tree showed that 2019-nCoV significantly clustered with bat SARS-like coronavirus sequence isolated in 2015, whereas structural analysis revealed mutation in Spike Glycoprotein and nucleocapsid protein. From these results, the new 2019-nCoV is distinct from SARS virus, probably trasmitted from bats after mutation conferring ability to infect humans.	[Benvenuto, Domenico; Ciccozzi, Alessandra] Univ Campus Biomed Rome, Unit Med Stat & Mol Epidemiol, Rome, Italy; [Giovanetti, Marta; Ciccozzi, Massimo] Fundacao Oswaldo Cruz, Inst Oswaldo Cruz, Lab Flavivirus, Rio De Janeiro, Brazil; [Spoto, Silvia] Univ Campus Biomed Rome, Internal Med Unit, Rome, Italy; [Angeletti, Silvia] Univ Campus Biomed Rome, Unit Clin Lab Sci, I-00128 Rome, Italy	Angeletti, S (reprint author), Univ Campus Biomed Rome, Unit Clin Lab Sci, I-00128 Rome, Italy.	s.angeletti@unicampus.it	; ciccozzi, massimo/C-6484-2016	Benvenuto, Domenico/0000-0003-3833-2927; ciccozzi, massimo/0000-0003-3866-9239			Benkert P, 2009, NUCLEIC ACIDS RES, V37, pW510, DOI 10.1093/nar/gkp322; Chakraborty S, 2017, GENE, V604, P48, DOI 10.1016/j.gene.2016.11.023; Chan JFW, 2020, LANCET, V395, P514, DOI 10.1016/S0140-6736(20)30154-9; Chen Y, 2020, J MED VIROL, V92, P418, DOI 10.1002/jmv.25681; Darriba D, 2012, NAT METHODS, V9, P772, DOI 10.1038/nmeth.2109; Drosten C, 2003, NEW ENGL J MED, V348, P1967, DOI 10.1056/NEJMoa030747; Hall T.A., 1999, NUCL ACIDS S SER, V41, P95, DOI DOI 10.1021/BK-1999-0734.CH008; Huang CL, 2020, LANCET, V395, P497, DOI 10.1016/S0140-6736(20)30183-5; Hui DS, 2020, INT J INFECT DIS, V91, P264, DOI 10.1016/j.ijid.2020.01.009; Ji W, 2020, J MED VIROL, V92, P433, DOI 10.1002/jmv.25682; Katoh K, 2019, BRIEF BIOINFORM, V20, P1160, DOI 10.1093/bib/bbx108; Kumar S, 2018, MOL BIOL EVOL, V35, P1547, DOI 10.1093/molbev/msy096; Lu HZ, 2020, J MED VIROL, V92, P401, DOI 10.1002/jmv.25678; Murrell B, 2013, MOL BIOL EVOL, V30, P1196, DOI 10.1093/molbev/mst030; Murrell B, 2012, PLOS GENET, V8, DOI 10.1371/journal.pgen.1002764; Schrodinger LLC, 2015, PYMOL MOL GRAPH SYST; Waterhouse A, 2018, NUCLEIC ACIDS RES, V46, pW296, DOI 10.1093/nar/gky427; Zhu N, 2020, NEW ENGL J MED, V382, P727, DOI 10.1056/NEJMoa2001017; Zimmermann L, 2018, J MOL BIOL, V430, P2237, DOI 10.1016/j.jmb.2017.12.007	19	4	4	116	116	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0146-6615	1096-9071		J MED VIROL	J. Med. Virol.	APR	2020	92	4			SI		455	459		10.1002/jmv.25688		FEB 2020	5	Virology	Virology	KM6QU	WOS:000511536300001	31994738	Bronze			2020-04-01	
J	Dey, SK; Rahman, MM; Siddiqi, UR; Howlader, A				Dey, Samrat K.; Rahman, Md. Mahbubur; Siddiqi, Umme R.; Howlader, Arpita			Analyzing the epidemiological outbreak of COVID-19: A visual exploratory data analysis approach	JOURNAL OF MEDICAL VIROLOGY			English	Article; Early Access						China; coronavirus; COVID-19; data analysis; SARS-CoV-2; visualization		There is an obvious concern globally regarding the fact about the emerging coronavirus 2019 novel coronavirus (2019-nCoV) as a worldwide public health threat. As the outbreak of COVID-19 causes by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) progresses within China and beyond, rapidly available epidemiological data are needed to guide strategies for situational awareness and intervention. The recent outbreak of pneumonia in Wuhan, China, caused by the SARS-CoV-2 emphasizes the importance of analyzing the epidemiological data of this novel virus and predicting their risks of infecting people all around the globe. In this study, we present an effort to compile and analyze epidemiological outbreak information on COVID-19 based on the several open datasets on 2019-nCoV provided by the Johns Hopkins University, World Health Organization, Chinese Center for Disease Control and Prevention, National Health Commission, and DXY. An exploratory data analysis with visualizations has been made to understand the number of different cases reported (confirmed, death, and recovered) in different provinces of China and outside of China. Overall, at the outset of an outbreak like this, it is highly important to readily provide information to begin the evaluation necessary to understand the risks and begin containment activities.	[Dey, Samrat K.] DIU, Dept Comp Sci & Engn, Dhaka 1205, Bangladesh; [Rahman, Md. Mahbubur] MIST, Dept Comp Sci & Engn, Dhaka, Bangladesh; [Siddiqi, Umme R.] Shaheed Suhrawardy Med Coll ShSMC, Dept Physiol, Dhaka, Bangladesh; [Howlader, Arpita] PSTU, Dept Comp & Commun Engn, Dumki, Bangladesh	Dey, SK (reprint author), DIU, Dept Comp Sci & Engn, Dhaka 1205, Bangladesh.	sopnil.samrat@gmail.com		Dey, Samrat Kumar/0000-0002-7999-8576			Centers for Disease Control and Prevention, 2019, 2019 NOV COR 2019 NC; Chen Y, 2020, J MED VIROL, V92, P418, DOI 10.1002/jmv.25681; Drosten C, 2003, NEW ENGL J MED, V348, P1967, DOI 10.1056/NEJMoa030747; Gorbalenya AE, 2020, ACUTE RESP SYNDROME, DOI [10.1101/2020.02.07.937862, DOI 10.1101/2020.02.07.937862]; Hui DS, 2020, INT J INFECT DIS, V91, P264, DOI 10.1016/j.ijid.2020.01.009; Ji W, 2020, J MED VIROL, V92, P433, DOI 10.1002/jmv.25682; Lauren G, 2020, CORONAVIRUS COVID 19; Lu HZ, 2020, J MED VIROL, V92, P401, DOI 10.1002/jmv.25678; World Health Organization, 2020, NOV COR CHIN; Yoo JH, 2020, J KOREAN MED SCI, V35, DOI 10.3346/jkms.2020.35.e56; Zhu N, 2020, NEW ENGL J MED, V382, P727, DOI 10.1056/NEJMoa2001017	11	1	1	108	108	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0146-6615	1096-9071		J MED VIROL	J. Med. Virol.												10.1002/jmv.25743		MAR 2020	7	Virology	Virology	KT5ZV	WOS:000519094100001	32124990	Bronze			2020-04-01	
J	Heymann, DL; Shindo, N; Bedford, J; Enria, D; Giesecke, J; Heymann, D; Ihekweazu, C; Kobinger, G; Lane, C; Memish, Z; Myoung-don, O; Sall, AA; Ungchusak, K; Wieler, L				Heymann, David L.; Shindo, Nahoko; Bedford, Juliet; Enria, Delia; Giesecke, Johan; Heymann, David; Ihekweazu, Chikwe; Kobinger, Gary; Lane, Clifford; Memish, Ziad; Myoung-don, Oh; Sall, Amadou Alpha; Ungchusak, Kum; Wieler, Lothar		WHO Sci Tech Advisory Grp Infect	COVID-19: what is next for public health?	LANCET			English	Editorial Material									[Heymann, David L.] London Sch Hyg &Trop Med, Infect Dis Epidemiol, London WC1E 7HT, England; [Shindo, Nahoko] WHO, Geneva, Switzerland; [Giesecke, Johan] Karolinska Inst, Stockholm, Sweden; [Ihekweazu, Chikwe] Nigeria Ctr Dis Control, Abuja, Nigeria; [Kobinger, Gary] Univ Laval, Infect Dis Res Ctr, Fac Med, Quebec City, PQ, Canada; [Lane, Clifford] NIAID, 9000 Rockville Pike, Bethesda, MD 20892 USA; [Memish, Ziad] Alfaisal Univ, Riyadh, Saudi Arabia; [Myoung-don, Oh] Seoul Natl Univ Hosp, JW Lee Ctr Global Med, SNU Coll Med, Dept Internal Med, Seoul, South Korea; [Sall, Amadou Alpha] Inst Pasteur, Unite Arbovirus & Virus Fievres Hemorrag, Dakar, Senegal; [Ungchusak, Kum] Minist Hlth, Dept Dis Control, Bangkok, Thailand; [Wieler, Lothar] Robert Koch Inst, Berlin, Germany	Heymann, DL (reprint author), London Sch Hyg &Trop Med, Infect Dis Epidemiol, London WC1E 7HT, England.	david.heymann@lshtm.ac.uk	Ribeiro, Nuno/AAH-2299-2020		World Health OrganizationWorld Health Organization [001]		Chan JFW, 2020, LANCET, V395, P514, DOI 10.1016/S0140-6736(20)30154-9; European Centre for Disease Prevention and Control, 2016, SEV AC RESP SYNDR AN; Lee SH, 2003, J EPIDEMIOL COMMUN H, V57, P652, DOI 10.1136/jech.57.9.652; Lu RJ, 2020, LANCET, V395, P565, DOI 10.1016/S0140-6736(20)30251-8; Peiris JSM, 2003, NEW ENGL J MED, V349, P2431, DOI 10.1056/NEJMra032498; WHO, 2003, DIS OUTBR NEWS AC RE; WHO, 2003, SARS UPD 84 CAN SARS; WHO, 2020, DIS OUTBREAK NE 0208; WHO, DIS OUTBR NEWS SEV A; World Health Organization, 2020, GLOB SURV HUM INF NO	10	3	3	103	103	ELSEVIER SCIENCE INC	NEW YORK	STE 800, 230 PARK AVE, NEW YORK, NY 10169 USA	0140-6736	1474-547X		LANCET	Lancet	FEB 22	2020	395	10224					542	545		10.1016/S0140-6736(20)30374-3			4	Medicine, General & Internal	General & Internal Medicine	KN5AJ	WOS:000514849400011	32061313	Bronze			2020-04-01	
J	Chen, LJ; Liu, WY; Zhang, Q; Xu, K; Ye, GM; Wu, WC; Sun, ZY; Liu, F; Wu, KL; Zhong, B; Mei, Y; Zhang, WX; Chen, Y; Li, YR; Shi, M; Lan, K; Liu, YL				Chen, Liangjun; Liu, Weiyong; Zhang, Qi; Xu, Ke; Ye, Guangming; Wu, Weichen; Sun, Ziyong; Liu, Fang; Wu, Kailang; Zhong, Bo; Mei, Yi; Zhang, Wenxia; Chen, Yu; Li, Yirong; Shi, Mang; Lan, Ke; Liu, Yingle			RNA based mNGS approach identifies a novel human coronavirus from two individual pneumonia cases in 2019 Wuhan outbreak	EMERGING MICROBES & INFECTIONS			English	Article						2019-nCoV; Wuhan pneumonia; metagenomic next-generation sequencing; phylogenetic analyses; virus evolution		From December 2019, an outbreak of unusual pneumonia was reported in Wuhan with many cases linked to Huanan Seafood Market that sells seafood as well as live exotic animals. We investigated two patients who developed acute respiratory syndromes after independent contact history with this market. The two patients shared common clinical features including fever, cough, and multiple ground-glass opacities in the bilateral lung field with patchy infiltration. Here, we highlight the use of a low-input metagenomic next-generation sequencing (mNGS) approach on RNA extracted from bronchoalveolar lavage fluid (BALF). It rapidly identified a novel coronavirus (named 2019-nCoV according to World Health Organization announcement) which was the sole pathogens in the sample with very high abundance level (1.5% and 0.62% of total RNA sequenced). The entire viral genome is 29,881 nt in length (GenBank MN988668 and MN988669, Sequence Read Archive database Bioproject accession PRJNA601736) and is classified into beta-coronavirus genus. Phylogenetic analysis indicates that 2019-nCoV is close to coronaviruses (CoVs) circulating in Rhinolophus (Horseshoe bats), such as 98.7% nucleotide identity to partial RdRp gene of bat coronavirus strain BtCoV/4991 (GenBank KP876546, 370 nt sequence of RdRp and lack of other genome sequence) and 87.9% nucleotide identity to bat coronavirus strain bat-SL-CoVZC45 and bat-SL-CoVZXC21. Evolutionary analysis based on ORF1a/1b, S, and N genes also suggests 2019-nCoV is more likely a novel CoV independently introduced from animals to humans.	[Chen, Liangjun; Zhang, Qi; Xu, Ke; Liu, Fang; Wu, Kailang; Zhong, Bo; Mei, Yi; Zhang, Wenxia; Chen, Yu; Lan, Ke; Liu, Yingle] Wuhan Univ, Coll Life Sci, Modern Virol Res Ctr, State Key Lab Virol, Wuhan, Peoples R China; [Chen, Liangjun; Ye, Guangming; Li, Yirong] Wuhan Univ, Dept Lab Med, Zhongnan Hosp, Wuhan, Peoples R China; [Liu, Weiyong; Sun, Ziyong] Huazhong Univ Sci & Technol, Tongji Med Coll, Tongji Hosp, Dept Lab Med, Wuhan, Peoples R China; [Wu, Weichen; Shi, Mang] Sun Yat Sen Univ, Sch Med, Guangzhou, Peoples R China	Chen, Y; Lan, K; Liu, YL (reprint author), Wuhan Univ, Coll Life Sci, Modern Virol Res Ctr, State Key Lab Virol, Wuhan, Peoples R China.; Li, YR (reprint author), Wuhan Univ, Dept Lab Med, Zhongnan Hosp, Wuhan, Peoples R China.; Shi, M (reprint author), Sun Yat Sen Univ, Sch Med, Guangzhou, Peoples R China.	chenyu@whu.edu.cn; liyirong838@163.com; shim23@mail.sysu.edu.cn; klan@whu.edu.cn; mvlwu@whu.edu.cn			National Mega Project on Major Infectious Disease Prevention [2017ZX10103005]; National Key Research and Development Program of China [2018YFE0204500]; National Science and Technology Major Project [2018ZX10733403]	This work was supported by the National Mega Project on Major Infectious Disease Prevention under [grant number 2017ZX10103005]; National Key Research and Development Program of China under [grant number 2018YFE0204500]; and National Science and Technology Major Project under [grant number 2018ZX10733403].	Buchfink B, 2015, NAT METHODS, V12, P59, DOI 10.1038/nmeth.3176; Chan JFW, 2020, EMERG MICROBES INFEC, V9, P221, DOI 10.1080/22221751.2020.1719902; Gu W, 2019, ANNU REV PATHOL-MECH, V14, P319, DOI 10.1146/annurev-pathmechdis-012418-012751; Guindon S, 2010, SYST BIOL, V59, P307, DOI 10.1093/sysbio/syq010; Hu B, 2017, PLOS PATHOG, V13, DOI 10.1371/journal.ppat.1006698; Jiang SB, 2020, EMERG MICROBES INFEC, V9, P275, DOI 10.1080/22221751.2020.1723441; Li DH, 2015, BIOINFORMATICS, V31, P1674, DOI 10.1093/bioinformatics/btv033; Lole KS, 1999, J VIROL, V73, P152, DOI 10.1128/JVI.73.1.152-160.1999; Menachery VD, 2015, NAT MED, V21, P1508, DOI 10.1038/nm.3985; Shi M, 2018, VIRUS RES, V243, P83, DOI 10.1016/j.virusres.2017.10.016; Shi M, 2017, J VIROL, V91, DOI 10.1128/JVI.00680-17; Truong DT, 2015, NAT METHODS, V12, P902, DOI 10.1038/nmeth.3589	12	5	5	102	102	TAYLOR & FRANCIS LTD	ABINGDON	2-4 PARK SQUARE, MILTON PARK, ABINGDON OR14 4RN, OXON, ENGLAND		2222-1751		EMERG MICROBES INFEC	Emerg. Microbes Infect.	JAN 1	2020	9	1					313	319		10.1080/22221751.2020.1725399			7	Immunology; Microbiology	Immunology; Microbiology	KK1XQ	WOS:000512543700001	32020836	DOAJ Gold, Green Published			2020-04-01	
J	Gralinski, LE; Menachery, VD				Gralinski, Lisa E.; Menachery, Vineet D.			Return of the Coronavirus: 2019-nCoV	VIRUSES-BASEL			English	Article						2019-nCoV; novel CoV; emerging viruses; Wuhan; Wuhan pneumonia; coronavirus; emerging viruses; SARS-CoV; MERS-CoV	RESPIRATORY-SYNDROME CORONAVIRUS; SARS; MERS; INFECTION; RECEPTOR; RHESUS; TRACT	The emergence of a novel coronavirus (2019-nCoV) has awakened the echoes of SARS-CoV from nearly two decades ago. Yet, with technological advances and important lessons gained from previous outbreaks, perhaps the world is better equipped to deal with the most recent emergent group 2B coronavirus.	[Gralinski, Lisa E.] Univ N Carolina, Gillings Sch Global Publ Hlth, Dept Epidemiol, Chapel Hill, NC 27514 USA; [Menachery, Vineet D.] Univ Texas Med Branch, Dept Microbiol & Immunol, Galveston, TX 77555 USA; [Menachery, Vineet D.] Univ Texas Med Branch, Inst Human Infect & Immun, Galveston, TX 77555 USA	Menachery, VD (reprint author), Univ Texas Med Branch, Dept Microbiol & Immunol, Galveston, TX 77555 USA.; Menachery, VD (reprint author), Univ Texas Med Branch, Inst Human Infect & Immun, Galveston, TX 77555 USA.	lgralins@email.unc.edu; Vimenach@utmb.edu	Ribeiro, Nuno/AAH-2299-2020	Menachery, Vineet/0000-0001-8803-7606			Al-Tawfiq JA, 2019, TRAVEL MED INFECT DI, V27, P27, DOI 10.1016/j.tmaid.2018.12.003; [Anonymous], STRAITS TIMES; Anthony SJ, 2017, MBIO, V8, DOI [10.1128/mBio.00373-17, 10.1128/mbio.00373-17]; Assiri A, 2013, LANCET INFECT DIS, V13, P752, DOI 10.1016/S1473-3099(13)70204-4; Azhar EI, 2014, NEW ENGL J MED, V370, P2499, DOI 10.1056/NEJMoa1401505; Becker MM, 2008, P NATL ACAD SCI USA, V105, P19944, DOI 10.1073/pnas.0808116105; Bedford T., GENOMIC EPIDEMIOLOGY; CIDRAP, MERS COV; CIDRAP, SARS SEV AC RESP SYN; Cockrell AS, 2014, J VIROL, V88, P5195, DOI 10.1128/JVI.03764-13; de Wit E, 2013, P NATL ACAD SCI USA, V110, P16598, DOI 10.1073/pnas.1310744110; Fehr AR, 2017, ANNU REV MED, V68, P387, DOI 10.1146/annurev-med-051215-031152; Hung IFN, 2004, EMERG INFECT DIS, V10, P1550, DOI 10.3201/eid1009.040058; Imai Natsuko, ESTIMATING POTENTIAL; Ji W, 2020, J MED VIROL; Kahn N., 2020, WALL STREET J; Kan B, 2005, J VIROL, V79, P11892, DOI 10.1128/JVI.79.18.11892-11900.2005; Kuiken T, 2003, LANCET, V362, P263, DOI 10.1016/S0140-6736(03)13967-0; Lau SKP, 2005, P NATL ACAD SCI USA, V102, P14040, DOI 10.1073/pnas.0506735102; Letko MMV, 2020, FUNCTIONAL ASSESSMEN; Li WH, 2007, VIROLOGY, V367, P367, DOI 10.1016/j.virol.2007.04.035; McAuliffe J, 2004, VIROLOGY, V330, P8, DOI 10.1016/j.virol.2004.09.030; Menachery VD, 2017, CURR OPIN VIROL, V23, P1, DOI 10.1016/j.coviro.2017.01.002; Menachery VD, 2016, P NATL ACAD SCI USA, V113, P3048, DOI 10.1073/pnas.1517719113; Menachery VD, 2015, NAT MED, V21, P1508, DOI 10.1038/nm.3985; Park D, 2016, CSH MOL CASE STUD, V2, DOI 10.1101/mcs.a001214; Quan PL, 2010, MBIO, V1, DOI 10.1128/mBio.00208-10; Rahman A, 2019, AM J PUBLIC HEALTH, V109, P1288, DOI [10.21, 10.2105/AJPH.2019.305186]; Rambaut A, 2020, PRELIMINARY PHYLOGEN; Rivers TM, 1937, J BACTERIOL, V33, P1; Roberts A, 2007, PLOS PATHOG, V3, P23, DOI 10.1371/journal.ppat.0030005; Robertson D., NCOVS RELATIONSHIP B; Shi Z.-L., 2020, DISCOVERY NOVEL CORO; Stein RA, 2011, INT J INFECT DIS, V15, pE510, DOI 10.1016/j.ijid.2010.06.020; van Doremalen N, 2014, J VIROL, V88, P9220, DOI 10.1128/JVI.00676-14; Virological.org, NOV 2019 COR GEN; Wang N, 2018, VIROL SIN, V33, P104, DOI 10.1007/s12250-018-0012-7; Wen X., 15 MED STAFF WUHAN C; Wong G, 2015, CELL HOST MICROBE, V18, P398, DOI 10.1016/j.chom.2015.09.013; World Health Organization, LAB TEST 2019 NOV CO; World Health Organization, WHO STAT REG CLUST P; World Health Organization, NOV COR JAP EX CHIN; Wuhan Municipal Health Commision, WUH MUN COMM HLTH HL; Wuhan Municipal Health Commision, WUH MUN HLTH HLTH CO; Xu YB, 2020, SCI CHINA LIFE SCI, V63, P450, DOI 10.1007/s11427-019-1630-2; Zeng LP, 2016, J VIROL, V90, P6573, DOI 10.1128/JVI.03079-15	46	8	8	101	101	MDPI	BASEL	ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND		1999-4915		VIRUSES-BASEL	Viruses-Basel	FEB	2020	12	2							135	10.3390/v12020135			8	Virology	Virology	KH2ZS	WOS:000510516800001	31991541	DOAJ Gold, Green Published			2020-04-01	
J	Wu, JT; Leung, K; Leung, GM				Wu, Joseph T.; Leung, Kathy; Leung, Gabriel M.			Nowcasting and forecasting the potential domestic and international spread of the 2019-nCoV outbreak originating in Wuhan, China: a modelling study	LANCET			English	Article							EAST RESPIRATORY SYNDROME; HUMAN CORONAVIRUSES 229E; HONG-KONG; TRANSMISSION DYNAMICS; SARS; EPIDEMIC; OC43; IMPACT	Background Since Dec 31, 2019, the Chinese city of Wuhan has reported an outbreak of atypical pneumonia caused by the 2019 novel coronavirus (2019-nCoV). Cases have been exported to other Chinese cities, as well as internationally, threatening to trigger a global outbreak. Here, we provide an estimate of the size of the epidemic in Wuhan on the basis of the number of cases exported from Wuhan to cities outside mainland China and forecast the extent of the domestic and global public health risks of epidemics, accounting for social and non-pharmaceutical prevention interventions. Methods We used data from Dec 31, 2019, to Jan 28, 2020, on the number of cases exported from Wuhan internationally (known days of symptom onset from Dec 25, 2019, to Jan 19, 2020) to infer the number of infections in Wuhan from Dec 1, 2019, to Jan 25, 2020. Cases exported domestically were then estimated. We forecasted the national and global spread of 2019-nCoV, accounting for the effect of the metropolitan-wide quarantine of Wuhan and surrounding cities, which began Jan 23-24, 2020. We used data on monthly flight bookings from the Official Aviation Guide and data on human mobility across more than 300 prefecture-level cities in mainland China from the Tencent database. Data on confirmed cases were obtained from the reports published by the Chinese Center for Disease Control and Prevention. Serial interval estimates were based on previous studies of severe acute respiratory syndrome coronavirus (SARS-CoV). A susceptible-exposed-infectious-recovered metapopulation model was used to simulate the epidemics across all major cities in China. The basic reproductive number was estimated using Markov Chain Monte Carlo methods and presented using the resulting posterior mean and 95% credibile interval (CrI). Findings In our baseline scenario, we estimated that the basic reproductive number for 2019-nCoV was 2.68 (95% CrI 2.47-2.86) and that 75 815 individuals (95% CrI 37 304-130 330) have been infected in Wuhan as of Jan 25, 2020. The epidemic doubling time was 6.4 days (95% CrI 5.8-7.1). We estimated that in the baseline scenario, Chongqing, Beijing, Shanghai, Guangzhou, and Shenzhen had imported 461 (95% CrI 227-805), 113 (57-193), 98 (49-168), 111 (56-191), and 80 (40-139) infections from Wuhan, respectively. If the transmissibility of 2019-nCoV were similar everywhere domestically and over time, we inferred that epidemics are already growing exponentially in multiple major cities of China with a lag time behind the Wuhan outbreak of about 1-2 weeks. Interpretation Given that 2019-nCoV is no longer contained within Wuhan, other major Chinese cities are probably sustaining localised outbreaks. Large cities overseas with close transport links to China could also become outbreak epicentres, unless substantial public health interventions at both the population and personal levels are implemented immediately. Independent self-sustaining outbreaks in major cities globally could become inevitable because of substantial exportation of presymptomatic cases and in the absence of large-scale public health interventions. Preparedness plans and mitigation interventions should be readied for quick deployment globally. Copyright (C) 2020 Elsevier Ltd. All rights reserved.	[Wu, Joseph T.; Leung, Kathy; Leung, Gabriel M.] Univ Hong Kong, Li Ka Shing Fac Med, Sch Publ Hlth, WHO Collaborating Ctr Infect Dis Epidemiol & Cont, Hong Kong, Peoples R China	Wu, JT (reprint author), Univ Hong Kong, Sch Publ Hlth, Li Ka Shing Fac Med, Hong Kong, Peoples R China.	joewu@hku.hk	; Wu, Joseph Tsz Kei/C-4450-2009; Leung, Kathy/D-5605-2017	Leung, Gabriel/0000-0002-2503-6283; Wu, Joseph Tsz Kei/0000-0002-3155-5987; Leung, Kathy/0000-0003-4777-388X	Health and Medical Research Fund from the Government of the Hong Kong Special Administrative Region	We thank Chi-Kin Lam and Miky Wong from School of Public Health, The University of Hong Kong (Hong Kong, China) for technical support. This research was supported by a commissioned grant from the Health and Medical Research Fund from the Government of the Hong Kong Special Administrative Region.	Abbad A, 2019, EUROSURVEILLANCE, V24; Al Kahlout RA, 2019, J IMMUNOL RES, DOI 10.1155/2019/1386740; [Anonymous], 2019, PEOPLES DAILY ONLINE; Bootsma MCJ, 2007, P NATL ACAD SCI USA, V104, P7588, DOI 10.1073/pnas.0611071104; Breban R, 2013, LANCET, V382, P694, DOI 10.1016/S0140-6736(13)61492-0; Cauchemez S, 2016, P NATL ACAD SCI USA, V113, P9081, DOI 10.1073/pnas.1519235113; Cauchemez S, 2014, LANCET INFECT DIS, V14, P50, DOI 10.1016/S1473-3099(13)70304-9; Centers for Disease Control and Prevention, 2005, FREQ ASK QUEST SARS; Chan CM, 2009, J CLIN VIROL, V45, P54, DOI 10.1016/j.jcv.2009.02.011; Chowell G, 2004, EMERG INFECT DIS, V10, P1258, DOI 10.3201/eid1007.030647; Chowell G, 2015, BMC MED, V13, DOI 10.1186/s12916-015-0450-0; Cowling BJ, 2015, EUROSURVEILLANCE, V20, P7, DOI 10.2807/1560-7917.ES2015.20.25.21163; Desai AN, 2019, HEALTH SECUR, V17, P268, DOI 10.1089/hs.2019.0022; Diekmann O, 2000, MATH EPIDEMIOLOGY IN; Gaunt ER, 2010, J CLIN MICROBIOL, V48, P2940, DOI 10.1128/JCM.00636-10; GILL EP, 1994, J CLIN MICROBIOL, V32, P2372, DOI 10.1128/JCM.32.10.2372-2376.1994; Hung LS, 2003, J ROY SOC MED, V96, P374, DOI 10.1258/jrsm.96.8.374; Imai N, 2020, MRC CTR GLOBAL INFEC, V1-3; Jia N, 2009, TROP MED INT HEALTH, V14, P21, DOI 10.1111/j.1365-3156.2008.02147.x; Kucharski AJ, 2015, EUROSURVEILLANCE, V20, P14, DOI 10.2807/1560-7917.ES2015.20.25.21167; Lau EHY, 2010, BMC INFECT DIS, V10, DOI 10.1186/1471-2334-10-50; Lessler J, 2009, LANCET INFECT DIS, V9, P291, DOI 10.1016/S1473-3099(09)70069-6; Leung GM, 2004, ANN INTERN MED, V141, P662, DOI 10.7326/0003-4819-141-9-200411020-00006; Leung GM, 2006, EPIDEMIOL INFECT, V134, P211, DOI 10.1017/S0950268805004826; Li Q, 2020, CHINA CDC WEEKLY, V2, P79; Li Qun, 2020, N Engl J Med, V382, P1199, DOI 10.1056/NEJMoa2001316; Lipsitch M, 2003, SCIENCE, V300, P1966, DOI 10.1126/science.1086616; Lipsitch M, 2011, BIOSECUR BIOTERROR, V9, P89, DOI 10.1089/bsp.2011.0007; Lloyd-Smith JO, 2005, NATURE, V438, P355, DOI 10.1038/nature04153; Matsuno AK, 2019, PLOS ONE, V14, DOI 10.1371/journal.pone.0217744; McDonald LC, 2004, EMERG INFECT DIS, V10, P777, DOI 10.3201/eid1005.030791; Oh MD, 2018, KOREAN J INTERN MED, V33, P233, DOI 10.3904/kjim.2018.031; Park JE, 2018, BMC PUBLIC HEALTH, V18, DOI 10.1186/s12889-018-5484-8; Patrick David M, 2006, Can J Infect Dis Med Microbiol, V17, P330; Polonsky JA, 2019, PHILOS T R SOC B, V374, DOI 10.1098/rstb.2018.0276; Pushkar D, 2010, PSYCHOL RES PROG, P5; Read JM, 2020, NOVEL CORONAVIRUS 20, DOI [10.1101/2020.01.23.20018549, DOI 10.1101/2020.01.23.20018549]; Riley S, 2003, SCIENCE, V300, P1961, DOI 10.1126/science.1086478; State Administration for Market Regulation, 2020, ANN STAT ADM MARK SU; T Liu, 2020, BIORXIV, DOI [10.1101/2020.01.25.919787, DOI 10.1101/2020.01.25.919787, https://doi.org/10.1101/2020.01.25.919787]; Vijgen L, 2005, J VIROL, V79, P1595, DOI 10.1128/JVI.79.3.1595-1604.2005; Virlogeux V, 2016, SCI REP-UK, V6, DOI 10.1038/srep35839; Walsh EE, 2013, J INFECT DIS, V208, P1634, DOI 10.1093/infdis/jit393; Wang L, 2018, NAT COMMUN, V9, DOI 10.1038/s41467-017-02344-z; Zhang SF, 2018, PLOS ONE, V13, DOI 10.1371/journal.pone.0191789; Zhao S, 2020, BIORXIV, DOI [10.1101/2020.01.23.916395 (preprint)., DOI 10.1101/2020.01.23.916395]	46	28	29	100	100	ELSEVIER SCIENCE INC	NEW YORK	STE 800, 230 PARK AVE, NEW YORK, NY 10169 USA	0140-6736	1474-547X		LANCET	Lancet	FEB 29	2020	395	10225					689	697		10.1016/S0140-6736(20)30260-9			9	Medicine, General & Internal	General & Internal Medicine	KQ2JO	WOS:000516755200027	32014114	Bronze			2020-04-01	
J	Chen, PJ; Mao, LJ; Nassis, GP; Harmer, P; Ainsworth, BE; Li, FZ				Chen, Peijie; Mao, Lijuan; Nassis, George P.; Harmer, Peter; Ainsworth, Barbara E.; Li, Fuzhong			Coronavirus disease (COVID-19): The need to maintain regular physical activity while taking precautions	JOURNAL OF SPORT AND HEALTH SCIENCE			English	Editorial Material									[Chen, Peijie; Mao, Lijuan; Nassis, George P.; Ainsworth, Barbara E.] Shanghai Univ Sport, Shanghai 200438, Peoples R China; [Mao, Lijuan] Shanghai Municipal Educ Commiss, Shanghai 200003, Peoples R China; [Nassis, George P.] Univ Southern Denmark, Dept Sports Sci & Clin Biomech, DK-5230 Odense, Denmark; [Harmer, Peter] Willamette Univ, Dept Exercise & Hlth Sci, Salem, OR 97301 USA; [Ainsworth, Barbara E.] Arizona State Univ, Sch Nutr & Hlth Promot, Phoenix, AZ 85004 USA; [Li, Fuzhong] Oregon Res Inst, Eugene, OR 97403 USA	Chen, PJ (reprint author), Shanghai Univ Sport, Shanghai 200438, Peoples R China.	chenpeijie@sus.edu.cn					[Anonymous], 2020, NEWS CHINA; Asia & Pacific, 2020, TRAV BAN GOES EFF CH; Blair SN, 2019, J SPORT HEALTH SCI, V8, P524, DOI 10.1016/j.jshs.2019.09.006; Callaway E, 2020, NATURE, V577, P605, DOI 10.1038/d41586-020-00166-6; Centers for Disease Control and Prevention, INT GUID PERS WHO MA; Guo YC, 2014, J SPORT HEALTH SCI, V3, P3, DOI 10.1016/j.jshs.2013.10.004; Health Emergency Office National Health Commission of the People's Republic of China, OUTBR NOT UPD PNEUM; Johns Hopkins University, COR 2019 NCOV GLOB C; Khaliq R., CHINA PROLONGS SCH C; Luan X, 2019, J SPORT HEALTH SCI, V8, P422, DOI 10.1016/j.jshs.2019.04.002; National Center for Complementary and Integrative Health, YOG WHAT YOU NEED KN; National Health Commission of the People's Republic of China, NOT PRINT DISTR GUID; Owen N, 2010, MAYO CLIN PROC, V85, P1138, DOI 10.4065/mcp.2010.0444; Stoye M., 2020, NATURE, DOI [10.1038/d41586-020-00253-8, DOI 10.1038/D41586-020-00253-8]; Tate Deborah F, 2015, Diabetes Spectr, V28, P45, DOI 10.2337/diaspect.28.1.45; United States Department of Health and Human Services, 2018, PHYS ACT GUID AM; World Health Organization, 2019, NOV COR 2019 NCOV SI; World Health Organization, NOV COR 2019 NCOV AD; World Health Organization, 2005, STAT 2 M INT HLTH RE; XINHUANET, XINH HEADL CHIN MOB	20	0	0	99	99	SHANGHAI UNIV SPORT	SHANGHAI	EDITORIAL BOARD, 650 QINGYUANHUAN RD, SHANGHAI, 200438, PEOPLES R CHINA	2095-2546	2213-2961		J SPORT HEALTH SCI	J. Sport Health Sci.	MAR	2020	9	2					103	104		10.1016/j.jshs.2020.02.001			2	Hospitality, Leisure, Sport & Tourism; Sport Sciences	Social Sciences - Other Topics; Sport Sciences	KN4WL	WOS:000514839200001	32099716	DOAJ Gold, Green Published			2020-04-01	
J	Xu, Y; Li, XF; Zhu, B; Liang, HY; Fang, CX; Gong, Y; Guo, QZ; Sun, X; Zhao, DY; Shen, J; Zhang, HY; Liu, HS; Xia, HM; Tang, JL; Zhang, K; Gong, ST				Xu, Yi; Li, Xufang; Zhu, Bing; Liang, Huiying; Fang, Chunxiao; Gong, Yu; Guo, Qiaozhi; Sun, Xin; Zhao, Danyang; Shen, Jun; Zhang, Huayan; Liu, Hongsheng; Xia, Huimin; Tang, Jinling; Zhang, Kang; Gong, Sitang			Characteristics of pediatric SARS-CoV-2 infection and potential evidence for persistent fecal viral shedding	NATURE MEDICINE			English	Article; Early Access							CHINA	We report epidemiological and clinical investigations on ten pediatric SARS-CoV-2 infection cases confirmed by real-time reverse transcription PCR assay of SARS-CoV-2 RNA. Symptoms in these cases were nonspecific and no children required respiratory support or intensive care. Chest X-rays lacked definite signs of pneumonia, a defining feature of the infection in adult cases. Notably, eight children persistently tested positive on rectal swabs even after nasopharyngeal testing was negative, raising the possibility of fecal-oral transmission. Children infected with the COVID-19 outbreak coronavirus, SARS-CoV-2, show mild symptoms but prolonged shedding of viral RNA in feces, suggesting that the fecal-oral route might play a role in virus transmission.	[Xu, Yi; Li, Xufang; Fang, Chunxiao; Gong, Yu; Shen, Jun; Zhang, Huayan; Gong, Sitang] Guangzhou Med Univ, Dept Pediat, Guangzhou Women & Childrens Med Ctr, Guangzhou, Peoples R China; [Li, Xufang] Guangzhou Med Univ, Dept Ctr Lab, Guangzhou Women & Childrens Med Ctr, Guangzhou, Peoples R China; [Zhu, Bing; Xia, Huimin; Tang, Jinling] Guangzhou Med Univ, Guangzhou Women & Childrens Med Ctr, Clin Data Ctr, Guangzhou, Peoples R China; [Liang, Huiying; Zhang, Huayan; Xia, Huimin; Tang, Jinling; Gong, Sitang] Guangzhou Med Univ, Guangdong Prov Childrens Med Res Ctr, Guangzhou Women & Childrens Med Ctr, Guangzhou, Peoples R China; [Guo, Qiaozhi; Sun, Xin; Zhao, Danyang] Guangzhou Med Univ, Dept Med Adm, Guangzhou Women & Childrens Med Ctr, Guangzhou, Peoples R China; [Zhang, Huayan] Childrens Hosp Philadelphia, Div Neonatol, Philadelphia, PA 19104 USA; [Zhang, Huayan] Childrens Hosp Philadelphia, Dept Pediat, Philadelphia, PA 19104 USA; [Zhang, Huayan] Univ Penn, Philadelphia, PA 19104 USA; [Liu, Hongsheng] Guangzhou Med Univ, Dept Radiol, Guangzhou Women & Childrens Med Ctr, Guangzhou, Peoples R China; [Xia, Huimin; Zhang, Kang] Guangzhou Med Univ, Guangzhou Women & Childrens Med Ctr, Guangdong Prov Key Lab Res Struct Birth Defect Di, Guangzhou, Peoples R China; [Zhang, Kang] Guangzhou Regenerat Med & Hlth Guangdong Lab, Guangzhou, Peoples R China; [Zhang, Kang] Macau Univ Sci & Technol, Fac Med, Ctr Biomed & Innovat, Macau, Peoples R China	Gong, ST (reprint author), Guangzhou Med Univ, Dept Pediat, Guangzhou Women & Childrens Med Ctr, Guangzhou, Peoples R China.; Xia, HM; Tang, JL (reprint author), Guangzhou Med Univ, Guangzhou Women & Childrens Med Ctr, Clin Data Ctr, Guangzhou, Peoples R China.; Xia, HM; Tang, JL; Gong, ST (reprint author), Guangzhou Med Univ, Guangdong Prov Childrens Med Res Ctr, Guangzhou Women & Childrens Med Ctr, Guangzhou, Peoples R China.; Xia, HM; Zhang, K (reprint author), Guangzhou Med Univ, Guangzhou Women & Childrens Med Ctr, Guangdong Prov Key Lab Res Struct Birth Defect Di, Guangzhou, Peoples R China.; Zhang, K (reprint author), Guangzhou Regenerat Med & Hlth Guangdong Lab, Guangzhou, Peoples R China.; Zhang, K (reprint author), Macau Univ Sci & Technol, Fac Med, Ctr Biomed & Innovat, Macau, Peoples R China.	huiminxia@hotmail.com; jltang@cuhk.edu.hk; kang.zhang@gmail.com; sitangg@126.com			National Key Research and Development Program of China [2019YFB1404803]; Guangzhou Regenerative Medicine Guangdong Laboratory [2020GZR110306001]; CDCs of Guangzhou CityUnited States Department of Health & Human ServicesCenters for Disease Control & Prevention - USA	This work was funded by the National Key Research and Development Program of China (2019YFB1404803) and Guangzhou Regenerative Medicine Guangdong Laboratory (2020GZR110306001). We thank the CDCs of Guangzhou City and Guangdong Province for helping with confirmation of the diagnosis of the viral infection.	[Anonymous], 2020, 36 WHO, P2020; Chen NS, 2020, LANCET, V395, P507, DOI 10.1016/S0140-6736(20)30211-7; Guan Wei-Jie, 2020, N Engl J Med, DOI 10.1056/NEJMoa2002032; Holshue ML, 2020, NEW ENGL J MED, V382, P929, DOI 10.1056/NEJMoa2001191; Huang CL, 2020, LANCET, V395, P497, DOI 10.1016/S0140-6736(20)30183-5; Munster VJ, 2020, NEW ENGL J MED, V382, P692, DOI 10.1056/NEJMp2000929; Wang C, 2020, LANCET, V395, P470, DOI 10.1016/S0140-6736(20)30185-9; Wang Dawei, 2020, JAMA, DOI 10.1001/jama.2020.1585; Zhu N, 2020, NEW ENGL J MED, V382, P727, DOI 10.1056/NEJMoa2001017; Zhu ZQ, 2015, J CLIN VIROL, V69, P30, DOI 10.1016/j.jcv.2015.05.013	10	0	0	97	97	NATURE PUBLISHING GROUP	NEW YORK	75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA	1078-8956	1546-170X		NAT MED	Nat. Med.												10.1038/s41591-020-0817-4		MAR 2020	9	Biochemistry & Molecular Biology; Cell Biology; Medicine, Research & Experimental	Biochemistry & Molecular Biology; Cell Biology; Research & Experimental Medicine	KU0SW	WOS:000519422600001		Bronze			2020-04-01	
J	Nishiura, H; Jung, SM; Linton, NM; Kinoshita, R; Yang, YC; Hayashi, K; Kobayashi, T; Yuan, BY; Akhmetzhanov, AR				Nishiura, Hiroshi; Jung, Sung-mok; Linton, Natalie M.; Kinoshita, Ryo; Yang, Yichi; Hayashi, Katsuma; Kobayashi, Tetsuro; Yuan, Baoyin; Akhmetzhanov, Andrei R.			The Extent of Transmission of Novel Coronavirus in Wuhan, China, 2020	JOURNAL OF CLINICAL MEDICINE			English	Editorial Material						epidemiology; foreigner; travel; migration; importation; emerging infectious diseases		A cluster of pneumonia cases linked to a novel coronavirus (2019-nCoV) was reported by China in late December 2019. Reported case incidence has now reached the hundreds, but this is likely an underestimate. As of 24 January 2020, with reports of thirteen exportation events, we estimate the cumulative incidence in China at 5502 cases (95% confidence interval: 3027, 9057). The most plausible number of infections is in the order of thousands, rather than hundreds, and there is a strong indication that untraced exposures other than the one in the epidemiologically linked seafood market in Wuhan have occurred.	[Nishiura, Hiroshi; Jung, Sung-mok; Linton, Natalie M.; Kinoshita, Ryo; Yang, Yichi; Hayashi, Katsuma; Kobayashi, Tetsuro; Yuan, Baoyin; Akhmetzhanov, Andrei R.] Hokkaido Univ, Grad Sch Med, Kita Ku, Kita 15 Jo Nishi 7 Chome, Sapporo, Hokkaido 0608638, Japan; [Nishiura, Hiroshi] Japan Sci & Technol Agcy, CREST, Honcho 4-1-8, Kawaguchi, Saitama 3320012, Japan	Nishiura, H (reprint author), Hokkaido Univ, Grad Sch Med, Kita Ku, Kita 15 Jo Nishi 7 Chome, Sapporo, Hokkaido 0608638, Japan.; Nishiura, H (reprint author), Japan Sci & Technol Agcy, CREST, Honcho 4-1-8, Kawaguchi, Saitama 3320012, Japan.	nishiurah@med.hokudai.ac.jp; seductmd@med.hokudai.ac.jp; nlinton@gmail.com; kinoshitaryo@gmail.com; lukeyang1993@eis.hokudai.ac.jp; katsuma5miffy@gmail.com; tootsieroll2910@gmail.com; baoyinyuan@gmail.com; akhmetzhanov@gmail.com	Akhmetzhanov, Andrei R./B-5530-2013	Akhmetzhanov, Andrei R./0000-0003-3269-7351; Jung, Sung-mok/0000-0002-0787-4515	Japan Agency for Medical Research and Development (AMED)Japan Agency for Medical Research and Development (AMED) [JP18fk0108050]; Japan Society for the Promotion of Science (JSPS) KAKENHIMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT)Japan Society for the Promotion of ScienceGrants-in-Aid for Scientific Research (KAKENHI) [17H04701, 17H05808, 18H04895, 19H01074, 18J21587]; Inamori Foundation; Japan Science and Technology Agency (JST) CREST program [JPMJCR1413]; Ministry of Education, Culture, Sports, Science and Technology, JapanMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT)	H.N. received funding from the Japan Agency for Medical Research and Development (AMED) [grant number: JP18fk0108050]; the Japan Society for the Promotion of Science (JSPS) KAKENHI [grant numbers, H.N.: 17H04701, 17H05808, 18H04895 and 19H01074; R.K.: 18J21587], the Inamori Foundation, and the Japan Science and Technology Agency (JST) CREST program [grant number: JPMJCR1413]. S.M.J. and N.M.L. received graduate study scholarship from the Ministry of Education, Culture, Sports, Science and Technology, Japan.	[Anonymous], 2020, XINHUA NEWS; [Anonymous], 2020, CHANNEL NEWS ASIA; [Anonymous], 2020, NIKKEI NEWS; [Anonymous], 2020, REUTERS WORLD NEWS; [Anonymous], 2020, CNN WORLD; [Anonymous], 2020, KOREA TIMES; [Anonymous], 2020, OUTBREAK PATIENTS PN; Bogoch Isaac I, 2020, J Travel Med, V27, DOI 10.1093/jtm/taaa008; CEIC, 2020, GLOB EC DAT; Centers for Disease Control and Prevention, 2020, 2019 NOV COR; Cui J, 2019, NAT REV MICROBIOL, V17, P181, DOI 10.1038/s41579-018-0118-9; Dorigatti I, 2017, EUROSURVEILLANCE, V22, P10, DOI 10.2807/1560-7917.ES.2017.22.28.30572; Hui DS, 2020, INT J INFECT DIS, V91, P264, DOI 10.1016/j.ijid.2020.01.009; Imperial College London-MRC Centre for Global Infectious Disease Analysis, 2020, NEWS WUHAN CORONAVIR; Kaixian TV, 2020, KAIXIAN TV; Lessler J, 2009, LANCET INFECT DIS, V9, P291, DOI 10.1016/S1473-3099(09)70069-6; Ministry of Health Singapore, 2020, CONF IMP CAS NOV COR; Northeastern University, 2020, LAB MOD BIOL SOC SYS; Shimizu K, 2019, J CLIN MED, V8, DOI 10.3390/jcm8060804; Thailand Ministry of Public Health-Bureau of Information Office of the Permanent Secretary of MOPH, 2020, MIN PUBL HLTH REV 2; The University of Hong Kong-HKUMed WHO Collaborating Centre for Infectious Disease Epidemiology and Control, 2020, NOWC FOR WUH 2019 NC; Tsuzuki S, 2017, EUROSURVEILLANCE, V22, P2, DOI 10.2807/1560-7917.ES.2017.22.46.17-00710; World Health Organization, 2020, 1 WHO; World Health Organization, 2020, NOV COR THAIL EXCH; World Health Organization, 2020, NOV COR JAP EXCH; World Health Organization, 2020, NOV COR REP KOR EXCH; Wuhan Municipal Health Commission, 2020, WUH MUN HLTH COMM BR	27	13	13	96	96	MDPI	BASEL	ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND		2077-0383		J CLIN MED	J. Clin. Med.	FEB	2020	9	2							330	10.3390/jcm9020330			5	Medicine, General & Internal	General & Internal Medicine	KH2ZW	WOS:000510517200001	31991628	DOAJ Gold, Green Published			2020-04-01	
J	Wax, RS; Christian, MD				Wax, Randy S.; Christian, Michael D.			Practical recommendations for critical care and anesthesiology teams caring for novel coronavirus (2019-nCoV) patients	CANADIAN JOURNAL OF ANESTHESIA-JOURNAL CANADIEN D ANESTHESIE			English	Review; Early Access						COVID-19	TRANSMISSION; SARS; ILLNESS	A global health emergency has been declared by the World Health Organization as the 2019-nCoV outbreak spreads across the world, with confirmed patients in Canada. Patients infected with 2019-nCoV are at risk for developing respiratory failure and requiring admission to critical care units. While providing optimal treatment for these patients, careful execution of infection control measures is necessary to prevent nosocomial transmission to other patients and to healthcare workers providing care. Although the exact mechanisms of transmission are currently unclear, human-to-human transmission can occur, and the risk of airborne spread during aerosol-generating medical procedures remains a concern in specific circumstances. This paper summarizes important considerations regarding patient screening, environmental controls, personal protective equipment, resuscitation measures (including intubation), and critical care unit operations planning as we prepare for the possibility of new imported cases or local outbreaks of 2019-nCoV. Although understanding of the 2019-nCoV virus is evolving, lessons learned from prior infectious disease challenges such as Severe Acute Respiratory Syndrome will hopefully improve our state of readiness regardless of the number of cases we eventually manage in Canada.	[Wax, Randy S.] Queens Univ, Fac Hlth Sci, Dept Crit Care Med, Kingston, ON, Canada; [Wax, Randy S.] Univ Toronto, Fac Med, Dept Med, Toronto, ON, Canada; [Wax, Randy S.] Lakeridge Hlth, Dept Crit Care Med, 1 Hosp Court, Oshawa, ON L1G 2B9, Canada; [Christian, Michael D.] Barts Hlth NHS Trust, Royal London Hosp, Londons Air Ambulance, London, England	Wax, RS (reprint author), Queens Univ, Fac Hlth Sci, Dept Crit Care Med, Kingston, ON, Canada.	randy.wax@queensu.ca	Ribeiro, Nuno/AAH-2299-2020				Abrahamson SD, 2006, CRIT CARE, V10, DOI 10.1186/cc3916; Bogoch Isaac I, 2020, J Travel Med, V27, DOI 10.1093/jtm/taaa011; Brest M, WASHINGTON EXAMINER; Buckley C, NY TIMES; Chan JFW, 2020, LANCET, V395, P514, DOI 10.1016/S0140-6736(20)30154-9; Cheung TMT, 2004, CHEST, V126, P845, DOI 10.1378/chest.126.3.845; Christian MD, 2004, CLIN INFECT DIS, V38, P1420, DOI 10.1086/420743; Christian MD, 2004, EMERG INFECT DIS, V10, P287, DOI 10.3201/eid1002.030700; Fan E, 2018, JAMA-J AM MED ASSOC, V319, P698, DOI 10.1001/jama.2017.21907; Fowler RA, 2003, JAMA-J AM MED ASSOC, V290, P367, DOI 10.1001/jama.290.3.367; Gottlieb Michael, 2019, Am J Emerg Med, DOI 10.1016/j.ajem.2019.12.019; Government of Canada, INF PREV CONTR NOV C; Government of Canada, INT NAT CAS DEF NOV; Government of Canada, GOV CAN EV CAN WUH C; Huang CL, 2020, LANCET, V395, P497, DOI 10.1016/S0140-6736(20)30183-5; Kupferschmidt K, 2020, SCIENCE; Leung CCH, 2019, J HOSP INFECT, V101, P84, DOI 10.1016/j.jhin.2018.10.007; Li Qun, 2020, N Engl J Med, V382, P1199, DOI 10.1056/NEJMoa2001316; Li Y, 2005, INDOOR AIR, V15, P83, DOI 10.1111/j.1600-0668.2004.00317.x; Loutfy MR, 2004, EMERG INFECT DIS, V10, P771, DOI 10.3201/eid1005.030717; Lu RJ, 2020, LANCET, V395, P565, DOI 10.1016/S0140-6736(20)30251-8; Majumder MS, 2014, PLOS CURR; McDonald LC, 2004, EMERG INFECT DIS, V10, P777, DOI 10.3201/eid1005.030791; Muller MP, 2006, CURR OPIN CRIT CARE, V12, P37, DOI 10.1097/01.ccx.0000198056.58083.a1; Nkengasong J, 2020, NAT MED, V26, P310, DOI 10.1038/s41591-020-0771-1; Novak D, 2015, USE EFFECTIVENESS PO; Rothe C, 2020, NEW ENGL J MED, V382, P970, DOI 10.1056/NEJMc2001468; Scales DC, 2003, EMERG INFECT DIS, V9, P1205, DOI 10.3201/eid0910.030525; Stelfox HT, 2003, JAMA-J AM MED ASSOC, V290, P1899, DOI 10.1001/jama.290.14.1899; Tien MHC, 2005, CAN J SURG, V48, P71; Warriner DR, 2009, BMJ-BRIT MED J, V338, DOI 10.1136/bmj.b521; World Health Organization, NOV COR NCOV 2019 SI; World Health Organization, WHO B; World Health Organization, 2005, EM COMM REG OUTBR NO; XINHUANET, NOV COR MAY SPREAD V; Yu ITS, 2004, NEW ENGL J MED, V350, P1731, DOI 10.1056/NEJMoa032867; Zamora JE, 2006, CAN MED ASSOC J, V175, P249, DOI 10.1503/cmaj.060094; Zhao Shi, 2020, Int J Infect Dis, V92, P214, DOI 10.1016/j.ijid.2020.01.050; Zhu N, 2020, NEW ENGL J MED, V382, P727, DOI 10.1056/NEJMoa2001017	39	2	2	92	92	SPRINGER	NEW YORK	ONE NEW YORK PLAZA, SUITE 4600, NEW YORK, NY, UNITED STATES	0832-610X	1496-8975		CAN J ANESTH	Can. J. Anesth.												10.1007/s12630-020-01591-x		FEB 2020	9	Anesthesiology	Anesthesiology	KK9FH	WOS:000513039800001	32052373	Bronze			2020-04-01	
J	Riou, J; Althaus, CL				Riou, Julien; Althaus, Christian L.			Pattern of early human-to-human transmission of Wuhan 2019 novel coronavirus (2019-nCoV), December 2019 to January 2020	EUROSURVEILLANCE			English	Article								Since December 2019, China has been experiencing a large outbreak of a novel coronavirus (2019-nCoV) which can cause respiratory disease and severe pneumonia. We estimated the basic reproduction number Ro of 2019-nCoV to be around 2.2 (90% high density interval: 1.4-3.8), indicating the potential for sustained human-to-human transmission. Transmission characteristics appear to be of similar magnitude to severe acute respiratory syndrome-related coronavirus (SARS-CoV) and pandemic influenza, indicating a risk of global spread.	[Riou, Julien; Althaus, Christian L.] Univ Bern, Inst Social & Prevent Med, Bern, Switzerland	Riou, J (reprint author), Univ Bern, Inst Social & Prevent Med, Bern, Switzerland.	julien.riou@ispm.unibe.ch	Ribeiro, Nuno/AAH-2299-2020		Swiss National Science FoundationSwiss National Science Foundation (SNSF) [174281]	JR is funded by the Swiss National Science Foundation (grant 174281).	Althaus CL, 2015, LANCET INFECT DIS, V15, P507, DOI 10.1016/S1473-3099(15)70135-0; Assiri A, 2013, NEW ENGL J MED, V369, P407, DOI 10.1056/NEJMoa1306742; Breban R, 2013, LANCET, V382, P694, DOI 10.1016/S0140-6736(13)61492-0; Chan JFW, 2020, LANCET, V395, P514, DOI 10.1016/S0140-6736(20)30154-9; Cowling BJ, 2008, EPIDEMIOL INFECT, V136, P562, DOI 10.1017/S0950268807008722; Fraser C, 2011, AM J EPIDEMIOL, V174, P505, DOI 10.1093/aje/kwr122; Huang C, 2020, LANCET, VS0140- 6736(20)30183-5, DOI [10.1016/S0140-6736(20)30154-9, DOI 10.1016/S0140-6736(20)30154-9]; Imai N, 2020, 2 IMP COLL; Kucharski AJ, 2015, EUROSURVEILLANCE, V20, P14, DOI 10.2807/1560-7917.ES2015.20.25.21167; Lloyd-Smith JO, 2005, NATURE, V438, P355, DOI 10.1038/nature04153; Oh MD, 2015, J KOREAN MED SCI, V30, P1701, DOI 10.3346/jkms.2015.30.11.1701; Organization WH, 2020, 9 WHO; P Zhou, 2020, BIORXIV, DOI [10.1101/2020.01.22.914952., DOI 10.1101/2020.01.22.914952, 10.1101/2020.01.22.914952]; R Core Team, 2018, R LANG ENV STAT COMP; Rambaut A, 2020, PRELIMINARY PHYLOGEN; World Health Organization, 2020, PNEUM UNKN CAUS CHIN	16	5	5	89	89	EUR CENTRE DIS PREVENTION & CONTROL	STOCKHOLM	TOMTEBODAVAGEN 11A, STOCKHOLM, 171 83, SWEDEN	1560-7917			EUROSURVEILLANCE	Eurosurveillance	JAN 30	2020	25	4					7	11		10.2807/1560-7917.ES.2020.25.4.2000058			5	Infectious Diseases	Infectious Diseases	KH4IF	WOS:000510607800002	32019669	DOAJ Gold, Green Published			2020-04-01	
J	Wu, F; Zhao, S; Yu, B; Chen, YM; Wang, W; Song, ZG; Hu, Y; Tao, ZW; Tian, JH; Pei, YY; Yuan, ML; Zhang, YL; Dai, FH; Liu, Y; Wang, QM; Zheng, JJ; Xu, L; Holmes, EC; Zhang, YZ				Wu, Fan; Zhao, Su; Yu, Bin; Chen, Yan-Mei; Wang, Wen; Song, Zhi-Gang; Hu, Yi; Tao, Zhao-Wu; Tian, Jun-Hua; Pei, Yuan-Yuan; Yuan, Ming-Li; Zhang, Yu-Ling; Dai, Fa-Hui; Liu, Yi; Wang, Qi-Min; Zheng, Jiao-Jiao; Xu, Lin; Holmes, Edward C.; Zhang, Yong-Zhen			A new coronavirus associated with human respiratory disease in China	NATURE			English	Article							MULTIPLE SEQUENCE ALIGNMENT; SARS-LIKE CORONAVIRUS; MAXIMUM-LIKELIHOOD; VIRUS; BATS; IDENTIFICATION; PERFORMANCE; DIVERSITY; HKU1	Emerging infectious diseases, such as severe acute respiratory syndrome (SARS) and Zika virus disease, present a major threat to public health(1-3). Despite intense research efforts, how, when and where new diseases appear are still a source of considerable uncertainty. A severe respiratory disease was recently reported in Wuhan, Hubei province, China. As of 25 January 2020, at least 1,975 cases had been reported since the first patient was hospitalized on 12 December 2019. Epidemiological investigations have suggested that the outbreak was associated with a seafood market in Wuhan. Here we study a single patient who was a worker at the market and who was admitted to the Central Hospital of Wuhan on 26 December 2019 while experiencing a severe respiratory syndrome that included fever, dizziness and a cough. Metagenomic RNA sequencing(4) of a sample of bronchoalveolar lavage fluid from the patient identified a new RNA virus strain from the family Coronaviridae, which is designated here 'WH-Human 1' coronavirus (and has also been referred to as '2019-nCoV'). Phylogenetic analysis of the complete viral genome (29,903 nucleotides) revealed that the virus was most closely related (89.1% nucleotide similarity) to a group of SARS-like coronaviruses (genus Betacoronavirus, subgenus Sarbecovirus) that had previously been found in bats in China(5). This outbreak highlights the ongoing ability of viral spill-over from animals to cause severe disease in humans.	[Wu, Fan; Chen, Yan-Mei; Song, Zhi-Gang; Pei, Yuan-Yuan; Zhang, Yu-Ling; Dai, Fa-Hui; Liu, Yi; Wang, Qi-Min; Zheng, Jiao-Jiao; Xu, Lin; Holmes, Edward C.; Zhang, Yong-Zhen] Fudan Univ, Shanghai Publ Hlth Clin Ctr, Shanghai, Peoples R China; [Zhao, Su; Hu, Yi; Tao, Zhao-Wu; Yuan, Ming-Li] Huazhong Univ Sci & Technol, Tongji Med Coll, Cent Hosp Wuhan, Dept Pulm & Crit Care Med, Wuhan, Peoples R China; [Yu, Bin; Tian, Jun-Hua] Wuhan Ctr Dis Control & Prevent, Wuhan, Peoples R China; [Wang, Wen; Zhang, Yong-Zhen] China Ctr Dis Control & Prevent, Natl Inst Communicable Dis Control & Prevent, Dept Zoonosis, Beijing, Peoples R China; [Holmes, Edward C.] Univ Sydney, Sch Life & Environm Sci, Marie Bashir Inst Infect Dis & Biosecur, Sydney, NSW, Australia; [Holmes, Edward C.] Univ Sydney, Sch Med Sci, Sydney, NSW, Australia; [Zhang, Yong-Zhen] Fudan Univ, Sch Publ Hlth, Shanghai, Peoples R China	Zhang, YZ (reprint author), Fudan Univ, Shanghai Publ Hlth Clin Ctr, Shanghai, Peoples R China.; Zhang, YZ (reprint author), China Ctr Dis Control & Prevent, Natl Inst Communicable Dis Control & Prevent, Dept Zoonosis, Beijing, Peoples R China.; Zhang, YZ (reprint author), Fudan Univ, Sch Publ Hlth, Shanghai, Peoples R China.	zhangyongzhen@shphc.org.cn			Special National Project on investigation of basic resources of China [SQ2019FY010009]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81861138003, 31930001]; ARC Australian Laureate FellowshipAustralian Research Council [FL170100022]	This study was supported by the Special National Project on investigation of basic resources of China (grant SQ2019FY010009) and the National Natural Science Foundation of China (grants 81861138003 and 31930001). E.C.H. is supported by an ARC Australian Laureate Fellowship (FL170100022).	Bermingham A, 2012, EUROSURVEILLANCE, V17, P6; Bolger AM, 2014, BIOINFORMATICS, V30, P2114, DOI 10.1093/bioinformatics/btu170; Cao Y, 2014, BIOINFORMATICS, V30, P1674, DOI 10.1093/bioinformatics/btu104; Drosten C, 2003, NEW ENGL J MED, V348, P1967, DOI 10.1056/NEJMoa030747; Edgar RC, 2004, NUCLEIC ACIDS RES, V32, P1792, DOI 10.1093/nar/gkh340; Ge XY, 2013, NATURE, V503, P535, DOI 10.1038/nature12711; Gorbalenya A. E, 2020, SEVERE ACUTE RESP SY, DOI [10.1101/2020.02.07.93786, DOI 10.1101/2020.02.07.93786]; Grabherr MG, 2011, NAT BIOTECHNOL, V29, P644, DOI 10.1038/nbt.1883; Guindon S, 2010, SYST BIOL, V59, P307, DOI 10.1093/sysbio/syq010; HAMRE D, 1966, P SOC EXP BIOL MED, V121, P190, DOI 10.3181/00379727-121-30734; Hu B, 2017, PLOS PATHOG, V13, DOI 10.1371/journal.ppat.1006698; Hu D, 2018, EMERG MICROBES INFEC, V7, DOI 10.1038/s41426-018-0155-5; Hulswit RJG, 2019, P NATL ACAD SCI USA, V116, P2681, DOI 10.1073/pnas.1809667116; Hwang WC, 2006, J BIOL CHEM, V281, P34610, DOI 10.1074/jbc.M603275200; Katoh K, 2013, MOL BIOL EVOL, V30, P772, DOI 10.1093/molbev/mst010; Langmead B, 2012, NAT METHODS, V9, P357, DOI [10.1038/nmeth.1923, 10.1038/NMETH.1923]; Lau SKP, 2005, P NATL ACAD SCI USA, V102, P14040, DOI 10.1073/pnas.0506735102; Li B, 2010, BIOINFORMATICS, V26, P493, DOI 10.1093/bioinformatics/btp692; Li F, 2005, SCIENCE, V309, P1864, DOI 10.1126/science.1116480; Li H, 2009, BIOINFORMATICS, V25, P1754, DOI 10.1093/bioinformatics/btp324; Li WD, 2005, SCIENCE, V310, P676, DOI 10.1126/science.1118391; Lin XD, 2017, VIROLOGY, V507, P1, DOI 10.1016/j.virol.2017.03.019; Lole KS, 1999, J VIROL, V73, P152, DOI 10.1128/JVI.73.1.152-160.1999; Martin DP, 2010, BIOINFORMATICS, V26, P2462, DOI 10.1093/bioinformatics/btq467; MCINTOSH K, 1967, P NATL ACAD SCI USA, V58, P2268, DOI 10.1073/pnas.58.6.2268; McMullan LK, 2019, LANCET INFECT DIS, V19, P1023, DOI 10.1016/S1473-3099(19)30291-9; Menachery VD, 2015, NAT MED, V21, P1508, DOI 10.1038/nm.3985; Ren W, 2008, J VIROL, V82, P1899, DOI 10.1128/JVI.01085-07; Shi M, 2018, NATURE, V556, P197, DOI 10.1038/s41586-018-0012-7; Shi M, 2016, NATURE, V540, P539, DOI 10.1038/nature20167; Tamura K, 2011, MOL BIOL EVOL, V28, P2731, DOI 10.1093/molbev/msr121; van der Hoek L, 2004, NAT MED, V10, P368, DOI 10.1038/nm1024; Ventura CV, 2016, LANCET, V387, P228, DOI 10.1016/S0140-6736(16)00006-4; Wang W, 2017, J VIROL, V91, DOI [10.1128/JVI.00764-17, 10.1128/jvi.00764-17]; Wang W, 2015, VIROLOGY, V474, P19, DOI 10.1016/j.virol.2014.10.017; Waterhouse A, 2018, NUCLEIC ACIDS RES, V46, pW296, DOI 10.1093/nar/gky427; WHO, 2020, WHO DIR GEN REM MED; Wolfe ND, 2007, NATURE, V447, P279, DOI 10.1038/nature05775; Woo PCY, 2005, J VIROL, V79, P884, DOI 10.1128/JVI.79.2.884-895.2005; Xu L, 2016, VIROL SIN, V31, P69, DOI 10.1007/s12250-016-3727-3; Yadav PD, 2019, EMERG INFECT DIS, V25, P1003, DOI 10.3201/eid2505.181076; Yang XL, 2016, J VIROL, V90, P3253, DOI 10.1128/JVI.02582-15; Zhou P, 2020, NATURE, DOI 10.1038/s41586-020-2012-7	43	14	14	83	83	NATURE PUBLISHING GROUP	LONDON	MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND	0028-0836	1476-4687		NATURE	Nature	MAR	2020	579	7798					265	+		10.1038/s41586-020-2008-3			17	Multidisciplinary Sciences	Science & Technology - Other Topics	KU0CC	WOS:000519378900029	32015508	Other Gold			2020-04-01	
J	Mahase, E				Mahase, Elisabeth			Coronavirus: covid-19 has killed more people than SARS and MERS combined, despite lower case fatality rate	BMJ-BRITISH MEDICAL JOURNAL			English	News Item												Ribeiro, Nuno/AAH-2299-2020				World Health Organization, MIDDL E RESP SYNDR C	1	2	2	83	83	BMJ PUBLISHING GROUP	LONDON	BRITISH MED ASSOC HOUSE, TAVISTOCK SQUARE, LONDON WC1H 9JR, ENGLAND	1756-1833			BMJ-BRIT MED J	BMJ-British Medical Journal	FEB 18	2020	368								m641	10.1136/bmj.m641			1	Medicine, General & Internal	General & Internal Medicine	KO0KR	WOS:000515235200008	32071063	Bronze			2020-04-01	
J	Wu, F; Zhao, S; Yu, B; Chen, YM; Wang, W; Song, ZG; Hu, Y; Tao, ZW; Tian, JH; Pei, YY; Yuan, ML; Zhang, YL; Dai, FH; Liu, Y; Wang, QM; Zheng, JJ; Xu, L; Holmes, EC; Zhang, YZ				Wu, Fan; Zhao, Su; Yu, Bin; Chen, Yan-Mei; Wang, Wen; Song, Zhi-Gang; Hu, Yi; Tao, Zhao-Wu; Tian, Jun-Hua; Pei, Yuan-Yuan; Yuan, Ming-Li; Zhang, Yu-Ling; Dai, Fa-Hui; Liu, Yi; Wang, Qi-Min; Zheng, Jiao-Jiao; Xu, Lin; Holmes, Edward C.; Zhang, Yong-Zhen			A new coronavirus associated with human respiratory disease in China	NATURE			English	Article; Early Access							MULTIPLE SEQUENCE ALIGNMENT; SARS-LIKE CORONAVIRUS; MAXIMUM-LIKELIHOOD; VIRUS; BATS; IDENTIFICATION; PERFORMANCE; DIVERSITY; HKU1	Emerging infectious diseases, such as severe acute respiratory syndrome (SARS) and Zika virus disease, present a major threat to public health(1-3). Despite intense research efforts, how, when and where new diseases appear are still a source of considerable uncertainty. A severe respiratory disease was recently reported in Wuhan, Hubei province, China. As of 25 January 2020, at least 1,975 cases had been reported since the first patient was hospitalized on 12 December 2019. Epidemiological investigations have suggested that the outbreak was associated with a seafood market in Wuhan. Here we study a single patient who was a worker at the market and who was admitted to the Central Hospital of Wuhan on 26 December 2019 while experiencing a severe respiratory syndrome that included fever, dizziness and a cough. Metagenomic RNA sequencing(4) of a sample of bronchoalveolar lavage fluid from the patient identified a new RNA virus strain from the family Coronaviridae, which is designated here 'WH-Human 1' coronavirus (and has also been referred to as '2019-nCoV'). Phylogenetic analysis of the complete viral genome (29,903 nucleotides) revealed that the virus was most closely related (89.1% nucleotide similarity) to a group of SARS-like coronaviruses (genus Betacoronavirus, subgenus Sarbecovirus) that had previously been found in bats in China(5). This outbreak highlights the ongoing ability of viral spill-over from animals to cause severe disease in humans. Phylogenetic and metagenomic analyses of the complete viral genome of a new coronavirus from the family Coronaviridae reveal that the virus is closely related to a group of SARS-like coronaviruses found in bats in China.	[Wu, Fan; Chen, Yan-Mei; Song, Zhi-Gang; Pei, Yuan-Yuan; Zhang, Yu-Ling; Dai, Fa-Hui; Liu, Yi; Wang, Qi-Min; Zheng, Jiao-Jiao; Xu, Lin; Holmes, Edward C.; Zhang, Yong-Zhen] Fudan Univ, Shanghai Publ Hlth Clin Ctr, Shanghai, Peoples R China; [Zhao, Su; Hu, Yi; Tao, Zhao-Wu; Yuan, Ming-Li] Huazhong Univ Sci & Technol, Dept Pulm & Crit Care Med, Tongji Med Coll, Cent Hosp Wuhan, Wuhan, Peoples R China; [Yu, Bin; Tian, Jun-Hua] Wuhan Ctr Dis Control & Prevent, Wuhan, Peoples R China; [Wang, Wen; Zhang, Yong-Zhen] China Ctr Dis Control & Prevent, Natl Inst Communicable Dis Control & Prevent, Dept Zoonosis, Beijing, Peoples R China; [Holmes, Edward C.] Univ Sydney, Marie Bashir Inst Infect Dis & Biosecur, Sch Life & Environm Sci, Sydney, NSW, Australia; [Holmes, Edward C.] Univ Sydney, Sch Med Sci, Sydney, NSW, Australia; [Zhang, Yong-Zhen] Fudan Univ, Sch Publ Hlth, Shanghai, Peoples R China	Zhang, YZ (reprint author), Fudan Univ, Shanghai Publ Hlth Clin Ctr, Shanghai, Peoples R China.; Zhang, YZ (reprint author), China Ctr Dis Control & Prevent, Natl Inst Communicable Dis Control & Prevent, Dept Zoonosis, Beijing, Peoples R China.; Zhang, YZ (reprint author), Fudan Univ, Sch Publ Hlth, Shanghai, Peoples R China.	zhangyongzhen@shphc.org.cn			Special National Project on investigation of basic resources of China [SQ2019FY010009]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81861138003, 31930001]; ARC Australian Laureate FellowshipAustralian Research Council [FL170100022]	This study was supported by the Special National Project on investigation of basic resources of China (grant SQ2019FY010009) and the National Natural Science Foundation of China (grants 81861138003 and 31930001). E.C.H. is supported by an ARC Australian Laureate Fellowship (FL170100022).	Bermingham A, 2012, EUROSURVEILLANCE, V17, P6; Bolger AM, 2014, BIOINFORMATICS, V30, P2114, DOI 10.1093/bioinformatics/btu170; Cao Y, 2014, BIOINFORMATICS, V30, P1674, DOI 10.1093/bioinformatics/btu104; Drosten C, 2003, NEW ENGL J MED, V348, P1967, DOI 10.1056/NEJMoa030747; Edgar RC, 2004, NUCLEIC ACIDS RES, V32, P1792, DOI 10.1093/nar/gkh340; Ge XY, 2013, NATURE, V503, P535, DOI 10.1038/nature12711; Gorbalenya A. E, 2020, SEVERE ACUTE RESP SY, DOI [10.1101/2020.02.07.93786, DOI 10.1101/2020.02.07.93786]; Grabherr MG, 2011, NAT BIOTECHNOL, V29, P644, DOI 10.1038/nbt.1883; Guindon S, 2010, SYST BIOL, V59, P307, DOI 10.1093/sysbio/syq010; HAMRE D, 1966, P SOC EXP BIOL MED, V121, P190, DOI 10.3181/00379727-121-30734; Hu B, 2017, PLOS PATHOG, V13, DOI 10.1371/journal.ppat.1006698; Hu D, 2018, EMERG MICROBES INFEC, V7, DOI 10.1038/s41426-018-0155-5; Hulswit RJG, 2019, P NATL ACAD SCI USA, V116, P2681, DOI 10.1073/pnas.1809667116; Hwang WC, 2006, J BIOL CHEM, V281, P34610, DOI 10.1074/jbc.M603275200; Katoh K, 2013, MOL BIOL EVOL, V30, P772, DOI 10.1093/molbev/mst010; Langmead B, 2012, NAT METHODS, V9, P357, DOI [10.1038/nmeth.1923, 10.1038/NMETH.1923]; Lau SKP, 2005, P NATL ACAD SCI USA, V102, P14040, DOI 10.1073/pnas.0506735102; Li B, 2010, BIOINFORMATICS, V26, P493, DOI 10.1093/bioinformatics/btp692; Li F, 2005, SCIENCE, V309, P1864, DOI 10.1126/science.1116480; Li H, 2009, BIOINFORMATICS, V25, P1754, DOI 10.1093/bioinformatics/btp324; Li WD, 2005, SCIENCE, V310, P676, DOI 10.1126/science.1118391; Lin XD, 2017, VIROLOGY, V507, P1, DOI 10.1016/j.virol.2017.03.019; Lole KS, 1999, J VIROL, V73, P152, DOI 10.1128/JVI.73.1.152-160.1999; Martin DP, 2010, BIOINFORMATICS, V26, P2462, DOI 10.1093/bioinformatics/btq467; MCINTOSH K, 1967, P NATL ACAD SCI USA, V58, P2268, DOI 10.1073/pnas.58.6.2268; McMullan LK, 2019, LANCET INFECT DIS, V19, P1023, DOI 10.1016/S1473-3099(19)30291-9; Menachery VD, 2015, NAT MED, V21, P1508, DOI 10.1038/nm.3985; Ren W, 2008, J VIROL, V82, P1899, DOI 10.1128/JVI.01085-07; Shi M, 2018, NATURE, V556, P197, DOI 10.1038/s41586-018-0012-7; Shi M, 2016, NATURE, V540, P539, DOI 10.1038/nature20167; Tamura K, 2011, MOL BIOL EVOL, V28, P2731, DOI 10.1093/molbev/msr121; van der Hoek L, 2004, NAT MED, V10, P368, DOI 10.1038/nm1024; Ventura CV, 2016, LANCET, V387, P228, DOI 10.1016/S0140-6736(16)00006-4; Wang W, 2017, J VIROL, V91, DOI [10.1128/JVI.00764-17, 10.1128/jvi.00764-17]; Wang W, 2015, VIROLOGY, V474, P19, DOI 10.1016/j.virol.2014.10.017; Waterhouse A, 2018, NUCLEIC ACIDS RES, V46, pW296, DOI 10.1093/nar/gky427; WHO, 2020, WHO DIR GEN REM MED; Wolfe ND, 2007, NATURE, V447, P279, DOI 10.1038/nature05775; Woo PCY, 2005, J VIROL, V79, P884, DOI 10.1128/JVI.79.2.884-895.2005; Xu L, 2016, VIROL SIN, V31, P69, DOI 10.1007/s12250-016-3727-3; Yadav PD, 2019, EMERG INFECT DIS, V25, P1003, DOI 10.3201/eid2505.181076; Yang XL, 2016, J VIROL, V90, P3253, DOI 10.1128/JVI.02582-15; Zhou P, 2020, NATURE, DOI 10.1038/s41586-020-2012-7	43	14	14	83	83	NATURE PUBLISHING GROUP	LONDON	MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND	0028-0836	1476-4687		NATURE	Nature												10.1038/s41586-020-2008-3		FEB 2020	20	Multidisciplinary Sciences	Science & Technology - Other Topics	KS1VQ	WOS:000518098100002	32015508	Other Gold			2020-04-01	
J	Holshue, ML; DeBolt, C; Lindquist, S; Lofy, KH; Wiesman, J; Bruce, H; Spitters, C; Ericson, K; Wilkerson, S; Tural, A; Diaz, G; Cohn, A; Fox, L; Patel, A; Gerber, SI; Kim, L; Tong, SX; Lu, XY; Lindstrom, S; Pallansch, MA; Weldon, WC; Biggs, HM; Uyeki, TM; Pillai, SK				Holshue, Michelle L.; DeBolt, Chas; Lindquist, Scott; Lofy, Kathy H.; Wiesman, John; Bruce, Hollianne; Spitters, Christopher; Ericson, Keith; Wilkerson, Sara; Tural, Ahmet; Diaz, George; Cohn, Amanda; Fox, LeAnne; Patel, Anita; Gerber, Susan I.; Kim, Lindsay; Tong, Suxiang; Lu, Xiaoyan; Lindstrom, Steve; Pallansch, Mark A.; Weldon, William C.; Biggs, Holly M.; Uyeki, Timothy M.; Pillai, Satish K.		Washington State-nCoV Case Invest	First Case of 2019 Novel Coronavirus in the United States	NEW ENGLAND JOURNAL OF MEDICINE			English	Article								A healthy 35 year-old man who had visited Wuhan, China, presented with cough and fever that progressed to pneumonia. This report describes the diagnosis, clinical course, and management of the condition. The case highlights the importance of close coordination between clinicians and public health authorities at the local, state, and federal levels. An outbreak of novel coronavirus (2019-nCoV) that began in Wuhan, China, has spread rapidly, with cases now confirmed in multiple countries. We report the first case of 2019-nCoV infection confirmed in the United States and describe the identification, diagnosis, clinical course, and management of the case, including the patient's initial mild symptoms at presentation with progression to pneumonia on day 9 of illness. This case highlights the importance of close coordination between clinicians and public health authorities at the local, state, and federal levels, as well as the need for rapid dissemination of clinical information related to the care of patients with this emerging infection.	[Holshue, Michelle L.] Ctr Dis Control & Prevent, Epidem Intelligence Serv, Atlanta, GA USA; [Cohn, Amanda; Fox, LeAnne; Patel, Anita] Ctr Dis Control & Prevent, Natl Ctr Immunizat & Resp Dis, Atlanta, GA USA; [Gerber, Susan I.; Kim, Lindsay; Tong, Suxiang; Lu, Xiaoyan; Lindstrom, Steve; Pallansch, Mark A.; Weldon, William C.; Biggs, Holly M.] Ctr Dis Control & Prevent, Div Viral Dis, Atlanta, GA USA; [Uyeki, Timothy M.] Ctr Dis Control & Prevent, Influenza Div, Atlanta, GA USA; [Pillai, Satish K.] Ctr Dis Control & Prevent, Div Preparedness & Emerging Infect, Atlanta, GA USA; [Holshue, Michelle L.; DeBolt, Chas; Lindquist, Scott; Lofy, Kathy H.; Wiesman, John] Washington State Dept Hlth, Shoreline, WA USA; [Bruce, Hollianne; Spitters, Christopher] Snohomish Hlth Dist, Everett, WA USA; [Ericson, Keith] Providence Med Grp, Everett, WA USA; [Wilkerson, Sara; Tural, Ahmet; Diaz, George] Providence Reg Med Ctr, Everett, WA USA; [Spitters, Christopher] Univ Washington, Sch Med, Dept Med, Seattle, WA 98195 USA	Holshue, ML (reprint author), Washington State Dept Hlth Publ Hlth Labs, 1610 NE 150th St, Shoreline, WA 98155 USA.	michelle.holshue@doh.wa.gov					Centers for Disease Control and Prevention, 2020, INT GUID HEALTHC PRO; Centers for Disease Control and Prevention, 2020, INFECT CONTROL; Centers for Disease Control and Prevention, 2020, 2019 NOV COR; Centers for Disease Control and Prevention, 2020, 2019 NOV COR 2019 NC; Centers for Disease Control and Prevention, 2020, REAL TIM RT PCR PAN; Centers for Disease Control and Prevention, 2020, INT GUID COLL HANDL; Chan JF-W, 2020, LANCET; Chen N, 2020, LANCET; Huang C, 2020, LANCET; Johns Hopkins University CSSE, WUH COR 2019 NCOV GL; Mulangu S, 2019, NEW ENGL J MED, V381, P2293, DOI 10.1056/NEJMoa1910993; Phan LT, 2020, NEW ENGL J MED, V382, P872, DOI 10.1056/NEJMc2001272; Sheahan Timothy P, 2020, Nat Commun, V11, P222, DOI 10.1038/s41467-019-13940-6; Washington State Department of Health, NOV COR OUTBR 2020; World Health Organization, 2020, PNEUM UNK CAUS CHIN; World Health Organization, 2020, NOV COR CHIN; Zhu N, 2020, NEW ENGL J MED, V382, P727, DOI 10.1056/NEJMoa2001017	17	17	17	82	82	MASSACHUSETTS MEDICAL SOC	WALTHAM	WALTHAM WOODS CENTER, 860 WINTER ST,, WALTHAM, MA 02451-1413 USA	0028-4793	1533-4406		NEW ENGL J MED	N. Engl. J. Med.	MAR 5	2020	382	10					929	936		10.1056/NEJMoa2001191			8	Medicine, General & Internal	General & Internal Medicine	KT7TC	WOS:000519214900008	32004427	Bronze			2020-04-01	
J	Bassetti, M; Vena, A; Giacobbe, DR				Bassetti, Matteo; Vena, Antonio; Giacobbe, Daniele Roberto			The Novel Chinese Coronavirus (2019-nCoV) Infections: challenges for fighting the storm	EUROPEAN JOURNAL OF CLINICAL INVESTIGATION			English	Article									[Bassetti, Matteo; Vena, Antonio; Giacobbe, Daniele Roberto] Univ Genoa, Dept Hlth Sci, Genoa, Italy; [Bassetti, Matteo; Vena, Antonio; Giacobbe, Daniele Roberto] Osped Policlinico San Martino IRCCS, Clin Malattie Infett, Lgo R Benzi 10, I-16132 Genoa, Italy	Bassetti, M (reprint author), Osped Policlinico San Martino IRCCS, Clin Malattie Infett, Lgo R Benzi 10, I-16132 Genoa, Italy.	matteo.bassetti@unige.it	Ribeiro, Nuno/AAH-2299-2020	Giacobbe, Daniele Roberto/0000-0003-2385-1759			Callaway E, 2020, NATURE, DOI [10.1038/d41586-020-00180-8, DOI 10.1038/D41586-020-00180-8]; Corman VM, 2020, EUROSURVEILLANCE, V25, P23, DOI 10.2807/1560-7917.ES.2020.25.3.2000045; de Wit E, 2016, NAT REV MICROBIOL, V14, P523, DOI 10.1038/nrmicro.2016.81; Heymann DL, 2020, LANCET; Huang C, 2020, LANCET; Ji W, 2020, J MED VIROL; Mo Y, 2016, J ANTIMICROB CHEMOTH, V71, P3340, DOI 10.1093/jac/dkw338; Munster VJ, 2020, NEW ENGLAND J MED; Paules CI, 2020, JAMA; Xu XT, 2020, SCI CHINA LIFE SCI, V63, P457, DOI 10.1007/s11427-020-1637-5; Yu ITS, 2004, NEW ENGL J MED, V350, P1731, DOI 10.1056/NEJMoa032867; Zhu N, 2020, N ENGL J MED; Zumla A, 2015, LANCET, V386, P995, DOI 10.1016/S0140-6736(15)60454-8	13	0	0	82	82	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0014-2972	1365-2362		EUR J CLIN INVEST	Eur. J. Clin. Invest.	MAR	2020	50	3								10.1111/eci.13209		JAN 2020	4	Medicine, General & Internal; Medicine, Research & Experimental	General & Internal Medicine; Research & Experimental Medicine	KS9EZ	WOS:000510768300001	32003000	Bronze			2020-04-01	
J	Zhang, L; Liu, YH				Zhang, Lei; Liu, Yunhui			Potential interventions for novel coronavirus in China: A systematic review	JOURNAL OF MEDICAL VIROLOGY			English	Review						2019-CoV; coronavirus; COVID-19; MERS; potential interventions; SARS	ACUTE RESPIRATORY SYNDROME; INFECTIOUS-BRONCHITIS VIRUS; ALPHA-LIPOIC ACID; ANGIOTENSIN-CONVERTING ENZYME-2; HUMAN MONOCLONAL-ANTIBODY; SARS CORONAVIRUS; VITAMIN-A; CONVALESCENT PLASMA; NITRIC-OXIDE; COXSACKIEVIRUS B3	An outbreak of a novel coronavirus (COVID-19 or 2019-CoV) infection has posed significant threats to international health and the economy. In the absence of treatment for this virus, there is an urgent need to find alternative methods to control the spread of disease. Here, we have conducted an online search for all treatment options related to coronavirus infections as well as some RNA-virus infection and we have found that general treatments, coronavirus-specific treatments, and antiviral treatments should be useful in fighting COVID-19. We suggest that the nutritional status of each infected patient should be evaluated before the administration of general treatments and the current children's RNA-virus vaccines including influenza vaccine should be immunized for uninfected people and health care workers. In addition, convalescent plasma should be given to COVID-19 patients if it is available. In conclusion, we suggest that all the potential interventions be implemented to control the emerging COVID-19 if the infection is uncontrollable.	[Zhang, Lei; Liu, Yunhui] China Med Univ, Dept Neurosurg, Shengjing Hosp, 36 Sanhao St, Shenyang 110004, Liaoning, Peoples R China	Liu, YH (reprint author), China Med Univ, Dept Neurosurg, Shengjing Hosp, 36 Sanhao St, Shenyang 110004, Liaoning, Peoples R China.	liuyh@sj-hospital.org			Key Laboratory of Neurooncology in Liaoning Province, China [112-2400017005]	Project of Key Laboratory of Neurooncology in Liaoning Province, China, Grant/Award Number: 112-2400017005	Agostini ML, 2018, MBIO, V9, DOI 10.1128/mBio.00221-18; Akerstrom S, 2005, J VIROL, V79, P1966, DOI 10.1128/JVI.79.3.1966-1969.2005; Alghamdi IG, 2014, INT J GEN MED, V7, P417, DOI 10.2147/IJGM.S67061; Alves DS, 2004, BIOCHEM PHARMACOL, V68, P549, DOI 10.1016/j.bcp.2004.04.012; Arabi Y, 2015, SPRINGERPLUS, V4, DOI 10.1186/s40064-015-1490-9; ATHERTON JG, 1978, ARCH VIROL, V56, P195, DOI 10.1007/BF01317848; Awotiwon AA, 2017, COCHRANE DB SYST REV, DOI 10.1002/14651858.CD011177.pub3; Banerjee A, 2019, VIRUSES-BASEL, V11, DOI 10.3390/v11010041; BARNES PJ, 1995, ANN MED, V27, P389, DOI 10.3109/07853899509002592; Baumann CA, 1997, MECH AGEING DEV, V94, P85, DOI 10.1016/S0047-6374(96)01860-X; BAUR A, 1991, KLIN WOCHENSCHR, V69, P722, DOI 10.1007/BF01649442; Beck MA, 2001, FASEB J, V15, P1481, DOI 10.1096/fj.00-0721fje; BECK MA, 1995, NAT MED, V1, P433, DOI 10.1038/nm0595-433; Beck MA, 2000, P NUTR SOC, V59, P581, DOI 10.1017/S0029665100000823; Beck MA, 1997, J NUTR, V127, pS966, DOI 10.1093/jn/127.5.966S; BECK MA, 1994, J NUTR, V124, P345; BEGIN ME, 1989, PROSTAG LEUKOTR ESS, V37, P135, DOI 10.1016/0952-3278(89)90110-5; Bijlenga G, 2005, J INFECTION, V51, P263, DOI 10.1016/j.jinf.2005.04.010; Blaising J, 2014, ANTIVIR RES, V107, P84, DOI 10.1016/j.antiviral.2014.04.006; Bodmer BS, 2018, VIROLOGY, V521, P99, DOI 10.1016/j.virol.2018.05.028; Booth CM, 2003, JAMA-J AM MED ASSOC, V289, P2801, DOI 10.1001/jama.289.21.JOC30885; Boriskin YS, 2008, CURR MED CHEM, V15, P997, DOI 10.2174/092986708784049658; BUSSEL JB, 1995, IMMUNOL INVEST, V24, P451, DOI 10.3109/08820139509062794; Cai C, 2018, FRONT IMMUNOL, V9, DOI 10.3389/fimmu.2018.00723; Cavanagh D, 2003, AVIAN PATHOL, V32, P567, DOI 10.1080/03079450310001621198; Chan JFW, 2020, EMERG MICROBES INFEC, V9, P221, DOI 10.1080/22221751.2020.1719902; Channappanavar R, 2017, J IMMUNOL, V198, P4046, DOI 10.4049/jimmunol.1601896; Chen F, 2004, J CLIN VIROL, V31, P69, DOI 10.1016/j.jcv.2004.03.003; Chen LL, 2005, J VIROL, V79, P7095, DOI 10.1128/JVI.79.11.7095-7103.2005; Chen Y, 2020, J MED VIROL, V92, P418, DOI 10.1002/jmv.25681; Cheng Y, 2005, EUR J CLIN MICROBIOL, V24, P44, DOI 10.1007/s10096-004-1271-9; Chu CM, 2004, THORAX, V59, P252, DOI 10.1136/thorax.2003.012658; Cinatl J, 2003, LANCET, V361, P2045, DOI 10.1016/S0140-6736(03)13615-X; Cohen J, 2020, SCIENCE, V367, P234, DOI 10.1126/science.367.6475.234; Costantini C, 2019, FRONT ONCOL, V9, DOI 10.3389/fonc.2019.00873; Dalakas MC, 2003, NEUROLOGY, V60, P1736, DOI 10.1212/01.WNL.0000074394.15882.83; Dawar FU, 2017, CURR ISSUES MOL BIOL, V21, P1, DOI 10.21775/cimb.021.001; Dimitrov DS, 2003, CELL, V115, P652, DOI 10.1016/S0092-8674(03)00976-0; DUCHATEAU J, 1985, SURV IMMUNOL RES, V4, P94; DUCHATEAU J, 1983, IMMUNOL TODAY, V4, P213, DOI 10.1016/0167-5699(83)90028-2; El-Senousey HK, 2018, POULTRY SCI, V97, P30, DOI 10.3382/ps/pex298; Escriou N, 2014, VIROLOGY, V452, P32, DOI 10.1016/j.virol.2014.01.002; Field CJ, 2002, J LEUKOCYTE BIOL, V71, P16; Frantz PN, 2018, MICROBES INFECT, V20, P493, DOI 10.1016/j.micinf.2018.01.005; Galmes S, 2018, NUTRIENTS, V10, DOI 10.3390/nu10121919; Gao Zhan-cheng, 2003, Zhongguo Wei Zhong Bing Ji Jiu Yi Xue, V15, P332; Guillin OM, 2019, NUTRIENTS, V11, DOI 10.3390/nu11092101; Haagmans BL, 2004, NAT MED, V10, P290, DOI 10.1038/nm1001; Harthill M, 2011, BIOL TRACE ELEM RES, V143, P1325, DOI 10.1007/s12011-011-8977-1; Hemila H, 2003, J ANTIMICROB CHEMOTH, V52, P1049, DOI 10.1093/jac/dkh002; Hemila H, 1997, PEDIATR INFECT DIS J, V16, P836, DOI 10.1097/00006454-199709000-00003; Ho TY, 2007, ANTIVIR RES, V74, P92, DOI 10.1016/j.antiviral.2006.04.014; Holick MF, 2004, AM J CLIN NUTR, V80, p1678S, DOI 10.1093/ajcn/80.6.1678S; Holshue M.L, 2020, NEW ENGL J MED, DOI [10.1056/NEJMoa2001191, DOI 10.1056/NEJM0A2001191]; Hui D S C, 2005, Monaldi Arch Chest Dis, V63, P149; Jarvis B, 1998, DRUGS, V56, P147, DOI 10.2165/00003495-199856010-00013; Jayaweera JAAS, 2019, SCI REP-UK, V9, DOI 10.1038/s41598-019-49122-z; Jee J, 2013, AM J VET RES, V74, P1353, DOI 10.2460/ajvr.74.10.1353; Jo S, 2020, J ENZYM INHIB MED CH, V35, P145, DOI 10.1080/14756366.2019.1690480; Jo S, 2019, CHEM BIOL DRUG DES, V94, P2023, DOI 10.1111/cbdd.13604; JOFFE MI, 1983, J CLIN INVEST, V72, P971, DOI 10.1172/JCI111069; Jones HD, 2015, PLOS ONE, V10, DOI 10.1371/journal.pone.0123460; Kantoch Miroslaw, 2002, Rocz Panstw Zakl Hig, V53, P385; Karlberg J, 2004, AM J EPIDEMIOL, V159, P229, DOI 10.1093/aje/kwh056; Keil SD, 2016, TRANSFUSION, V56, P2948, DOI 10.1111/trf.13860; Khamitov R. A., 2008, Voprosy Virusologii, V53, P9; Kim UJ, 2016, ANTIVIR THER, V21, P455, DOI 10.3851/IMP3002; Kumar V, 2013, EXPERT OPIN THER PAT, V23, P1337, DOI 10.1517/13543776.2013.823159; Kuri T, 2009, J GEN VIROL, V90, P2686, DOI 10.1099/vir.0.013599-0; Kyme P, 2012, J CLIN INVEST, V122, P3316, DOI 10.1172/JCI62070; Leong HN, 2006, ANN ACAD MED SINGAP, V35, P326; Leu GZ, 2004, BIOCHEM BIOPH RES CO, V318, P275, DOI 10.1016/j.bbrc.2004.04.019; Lew TWK, 2003, JAMA-J AM MED ASSOC, V290, P374, DOI 10.1001/jama.290.3.374; Li QL, 2011, PEPTIDES, V32, P1518, DOI 10.1016/j.peptides.2011.05.015; Li Qun, 2020, N Engl J Med, V382, P1199, DOI 10.1056/NEJMoa2001316; Li WH, 2003, NATURE, V426, P450, DOI 10.1038/nature02145; Lin SC, 2017, BMC INFECT DIS, V17, DOI 10.1186/s12879-017-2253-8; Loutfy MR, 2003, JAMA-J AM MED ASSOC, V290, P3222, DOI 10.1001/jama.290.24.3222; Luo C, 2004, BIOCHEM BIOPH RES CO, V321, P557, DOI 10.1016/j.bbrc.2004.07.003; Ma X, 2019, POULTRY SCI, V98, P3548, DOI 10.3382/ps/pez207; Maares M, 2016, ARCH BIOCHEM BIOPHYS, V611, P58, DOI 10.1016/j.abb.2016.03.022; Manns MP, 2001, LANCET, V358, P958, DOI 10.1016/S0140-6736(01)06102-5; Marano G, 2016, BLOOD TRANSFUS-ITALY, V14, P152, DOI 10.2450/2015.0131-15; Marriott I, 2006, IMMUNOL RES, V34, P177, DOI 10.1385/IR:34:3:177; Matteucci C, 2017, FUTURE MICROBIOL, V12, P141, DOI 10.2217/fmb-2016-0125; Morgenstern B, 2005, BIOCHEM BIOPH RES CO, V326, P905, DOI 10.1016/j.bbrc.2004.11.128; Morita M, 2013, CELL, V153, P112, DOI 10.1016/j.cell.2013.02.027; Mustafa S, 2018, J INFECT PUBLIC HEAL, V11, P9, DOI 10.1016/j.jiph.2017.08.009; Nonnecke BJ, 2014, J DAIRY SCI, V97, P5566, DOI 10.3168/jds.2014-8293; Oyranoski D, 2003, NATURE, V423, P4, DOI 10.1038/423004a; Panche AN, 2016, J NUTR SCI, V5, DOI 10.1017/jns.2016.41; Park JY, 2012, BIOL PHARM BULL, V35, P2036, DOI 10.1248/bpb.b12-00623; Pecheur EI, 2016, J VIROL, V90, P3086, DOI 10.1128/JVI.02077-15; Pei JW, 2001, J INTERF CYTOK RES, V21, P1071, DOI 10.1089/107999001317205204; Peretz J, 2016, AM J PHYSIOL-LUNG C, V310, pL415, DOI 10.1152/ajplung.00398.2015; Pfefferle S, 2011, PLOS PATHOG, V7, DOI 10.1371/journal.ppat.1002331; Pica F, 2018, EXPERT OPIN BIOL TH, V18, P13, DOI 10.1080/14712598.2018.1474197; Powers HJ, 2003, AM J CLIN NUTR, V77, P1352; Rayman MP, 2012, LANCET, V379, P1256, DOI 10.1016/S0140-6736(11)61452-9; RENOUX G, 1980, DRUGS, V20, P89, DOI 10.2165/00003495-198020020-00001; Robbins RA, 1997, INT J BIOCHEM CELL B, V29, P857, DOI 10.1016/S1357-2725(96)00167-7; ROSSAINT R, 1995, CHEST, V107, P1107, DOI 10.1378/chest.107.4.1107; Ryu YB, 2010, BIOORGAN MED CHEM, V18, P7940, DOI 10.1016/j.bmc.2010.09.035; Sachse G, 1980, Horm Metab Res Suppl, V9, P105; Savarino A, 2003, LANCET INFECT DIS, V3, P722, DOI 10.1016/S1473-3099(03)00806-5; Schoeman D, 2019, VIROL J, V16, DOI 10.1186/s12985-019-1182-0; Semba RD, 1999, P NUTR SOC, V58, P719, DOI 10.1017/S0029665199000944; Sheahan Timothy P, 2020, Nat Commun, V11, P222, DOI 10.1038/s41467-019-13940-6; Shimizu JF, 2017, SCI REP-UK, V7, DOI 10.1038/s41598-017-16336-y; Simmons G, 2004, P NATL ACAD SCI USA, V101, P4240, DOI 10.1073/pnas.0306446101; Soo YOY, 2004, CLIN MICROBIOL INFEC, V10, P676, DOI 10.1111/j.1469-0691.2004.00956.x; Sui JH, 2004, P NATL ACAD SCI USA, V101, P2536, DOI 10.1073/pnas.0307140101; Takahashi S, 2015, BIOMED RES-TOKYO, V36, P219, DOI 10.2220/biomedres.36.219; Tan ELC, 2004, EMERG INFECT DIS, V10, P581, DOI 10.3201/eid1004.030458; Tangpricha V, 2002, AM J MED, V112, P659, DOI 10.1016/S0002-9343(02)01091-4; ter Meulen J, 2004, LANCET, V363, P2139, DOI 10.1016/S0140-6736(04)16506-9; ter Meulen J, 2006, PLOS MED, V3, P1071, DOI 10.1371/journal.pmed.0030237; Tibullo D, 2017, INFLAMM RES, V66, P947, DOI 10.1007/s00011-017-1079-6; Trampezynska A, 2006, FEBS LETT, V580, P3173, DOI 10.1016/j.febslet.2006.04.073; Trottier C, 2009, FASEB J, V23, P3203, DOI 10.1096/fj.09-129288; Tsang K, 2003, RESPIROLOGY, V8, pS25, DOI 10.1046/j.1440-1843.2003.00525.x; Tuerk MJ, 2009, CURR OPIN GASTROEN, V25, P136, DOI 10.1097/MOG.0b013e328321b395; TURNER RB, 1986, J INFECT DIS, V154, P443, DOI 10.1093/infdis/154.3.443; Velthuis AJWT, 2010, PLOS PATHOG, V6, DOI 10.1371/journal.ppat.1001176; Villamor E, 2002, PEDIATRICS, V109, DOI 10.1542/peds.109.1.e6; Vincent MJ, 2005, VIROL J, V2, DOI 10.1186/1743-422X-2-69; Warner FJ, 2004, CELL MOL LIFE SCI, V61, P2704, DOI 10.1007/s00018-004-4240-7; Wei L, 2010, BIOCHEM CELL BIOL, V88, P723, DOI 10.1139/O10-022; Wenzel RP, 2003, NEW ENGL J MED, V348, P1947, DOI 10.1056/NEJMp030072; Wessling-Resnick M, 2018, ANNU REV NUTR, V38, P431, DOI 10.1146/annurev-nutr-082117-051749; WEST CE, 1992, J NUTR, V122, P333; Wu YH, 2008, J INFECT DIS, V197, P812, DOI 10.1086/528377; Yamamoto N, 2004, BIOCHEM BIOPH RES CO, V318, P719, DOI 10.1016/j.bbrc.2004.04.083; Yeung KS, 2006, MED RES REV, V26, P414, DOI 10.1002/med.20055; ZARUBA K, 1983, LANCET, V2, P1245; Zhang NR, 2020, J MED VIROL, V92, P408, DOI 10.1002/jmv.25674; Zhang XW, 2004, BIOORGAN MED CHEM, V12, P2517, DOI 10.1016/j.bmc.2004.03.035; Zhu N, 2020, NEW ENGL J MED, V382, P727, DOI 10.1056/NEJMoa2001017; Ziaei M, 2016, INT OPHTHALMOL, V36, P139, DOI 10.1007/s10792-015-0137-8; Zumla A, 2015, LANCET, V386, P995, DOI 10.1016/S0140-6736(15)60454-8	140	1	1	81	81	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0146-6615	1096-9071		J MED VIROL	J. Med. Virol.	MAY	2020	92	5					479	490		10.1002/jmv.25707		MAR 2020	12	Virology	Virology	KU3AY	WOS:000517680100001	32052466	Bronze			2020-04-01	
J	Fong, SJ; Li, G; Dey, N; Crespo, RG; Herrera-Viedma, E				Fong, Simon James; Li, Gloria; Dey, Nilanjan; Gonzalez Crespo, Ruben; Herrera-Viedma, Enrique			Finding an Accurate Early Forecasting Model from Small Dataset: A Case of 2019-nCoV Novel Coronavirus Outbreak	INTERNATIONAL JOURNAL OF INTERACTIVE MULTIMEDIA AND ARTIFICIAL INTELLIGENCE			English	Article						Forecasting; Machine Learning; Method; Prediction; Data Mining; Epidemic	NEURAL-NETWORKS; SELECTION	Epidemic is a rapid and wide spread of infectious disease threatening many lives and economy damages. It is important to fore-tell the epidemic lifetime so to decide on timely and remedic actions. These measures include closing borders, schools, suspending community services and commuters. Resuming such curfews depends on the momentum of the outbreak and its rate of decay. Being able to accurately forecast the fate of an epidemic is an extremely important but difficult task. Due to limited knowledge of the novel disease, the high uncertainty involved and the complex societal-political factors that influence the widespread of the new virus, any forecast is anything but reliable. Another factor is the insufficient amount of available data. Data samples are often scarce when an epidemic just started. With only few training samples on hand, finding a forecasting model which offers forecast at the best efforts is a big challenge in machine learning. In the past, three popular methods have been proposed, they include 1) augmenting the existing little data, 2) using a panel selection to pick the best forecasting model from several models, and 3) fine-tuning the parameters of an individual forecasting model for the highest possible accuracy. In this paper, a methodology that embraces these three virtues of data mining from a small dataset is proposed. An experiment that is based on the recent coronavirus outbreak originated from Wuhan is conducted by applying this methodology. It is shown that an optimized forecasting model that is constructed from a new algorithm, namely polynomial neural network with corrective feedback (PNN+cf) is able to make a forecast that has relatively the lowest prediction error. The results showcase that the newly proposed methodology and PNN+cf are useful in generating acceptable forecast upon the critical time of disease outbreak when the samples are far from abundant.	[Fong, Simon James] Univ Macau, Dept Comp & Informat Sci, Macau, Peoples R China; [Fong, Simon James; Li, Gloria] Chinese Acad Sci, Zhuhai Inst Adv Technol, DACC Lab, Beijing, Peoples R China; [Dey, Nilanjan] Techno India Coll Technol, Dept Informat Technol, Kolkata, W Bengal, India; [Gonzalez Crespo, Ruben] Univ Int La Rioja, Logrono, Spain; [Herrera-Viedma, Enrique] Univ Granada, Granada, Spain	Fong, SJ (reprint author), Univ Macau, Dept Comp & Informat Sci, Macau, Peoples R China.; Fong, SJ (reprint author), Chinese Acad Sci, Zhuhai Inst Adv Technol, DACC Lab, Beijing, Peoples R China.; Dey, N (reprint author), Techno India Coll Technol, Dept Informat Technol, Kolkata, W Bengal, India.	ccfong@um.edu.mo; nilanjan.dey@tict.edu.in					Adhikari R., 2013, ARXIV13026613; Andoni A., 2014, P 31 INT C MACH LEAR, V32; Andonie R, 2010, INT J COMPUT COMMUN, V5, P280, DOI 10.15837/ijccc.2010.3.2481; Arun V, 2018, INT J INTERACT MULTI, V5, P61, DOI 10.9781/ijimai.2018.10.001; Croda RMC, 2019, INT J INTERACT MULTI, V5, P35, DOI 10.9781/ijimai.2018.04.003; Cohen Jon, 2020, SCIENCE, DOI [10.1126/science.abb0611, DOI 10.1126/SCIENCE.ABB0611]; Dey N., 2018, COMMUNICATIONS COMPU, V876; Fong S, 2012, INT CONF DAT MIN WOR, P464, DOI 10.1109/ICDMW.2012.67; Hyndman R.J., 2007, INT J APPL FORECAST, V6, P12; Ingrassia S, 2005, TECHNOMETRICS, V47, P297, DOI 10.1198/004017005000000058; Ivakhnenko A. G., 1992, Journal of Automation and Information Science, V25, P25; IVAKHNENKO AG, 1970, AUTOMATICA, V6, P207, DOI 10.1016/0005-1098(70)90092-0; Lateh M. A., J PHYS C SERIES, V892; Oh SK, 2003, COMPUT ELECTR ENG, V29, P703, DOI 10.1016/S0045-7906(02)00045-9; Pasini A, 2015, J THORAC DIS, V7, P953, DOI 10.3978/j.issn.2072-1439.2015.04.61; Shaikhina T, 2017, ARTIF INTELL MED, V75, P51, DOI 10.1016/j.artmed.2016.12.003; Shevade S. K., 1999, IEEE T NEURAL NETWOR; Singh A., 2015, STAT TRANSITION, V16, P83; SLIFKER JF, 1980, TECHNOMETRICS, V22, P239, DOI 10.2307/1268463; Sparrow Annie, FOREIGN POLICY; Su J., 2006, P 21 NAT C ART INT A, V1, P500; Waheeb W, 2019, INT J INTERACT MULTI, V5, P126, DOI 10.9781/ijimai.2019.04.004; Wang Y., 1997, 9 EUR C MACH LEARN; WHO, WHO NOV COR CHIN	24	0	0	80	80	UNIV INT RIOJA-UNIR	LOGRONO	RECTORADO, AVENIDA DE LA PAZ, 137, LOGRONO, 26006, SPAIN	1989-1660			INT J INTERACT MULTI	Int. J. Interact. Multimed. Artif. Intell.	MAR	2020	6	1					132	140		10.9781/ijimai.2020.02.002			9	Computer Science, Artificial Intelligence; Computer Science, Interdisciplinary Applications	Computer Science	KN4XF	WOS:000514841200016		DOAJ Gold			2020-04-01	
J	Wu, P; Hao, XX; Lau, EHY; Wong, JY; Leung, KSM; Wu, JT; Cowling, BJ; Leung, GM				Wu, Peng; Hao, Xinxin; Lau, Eric H. Y.; Wong, Jessica Y.; Leung, Kathy S. M.; Wu, Joseph T.; Cowling, Benjamin J.; Leung, Gabriel M.			Real-time tentative assessment of the epidemiological characteristics of novel coronavirus infections in Wuhan, China, as at 22 January 2020	EUROSURVEILLANCE			English	Article							ACUTE-RESPIRATORY-SYNDROME; CASE-FATALITY RATIO; MERS-COV OUTBREAK; SOUTH-KOREA	A novel coronavirus (2019-nCoV) causing severe acute respiratory disease emerged recently in Wuhan, China. Information on reported cases strongly indicates human-to-human spread, and the most recent information is increasingly indicative of sustained human-to-human transmission. While the overall severity profile among cases may change as more mild cases are identified, we estimate a risk of fatality among hospitalised cases at 14% (95% confidence interval: 3.9-32%).	[Wu, Peng; Hao, Xinxin; Lau, Eric H. Y.; Wong, Jessica Y.; Leung, Kathy S. M.; Wu, Joseph T.; Cowling, Benjamin J.; Leung, Gabriel M.] Univ Hong Kong, Sch Publ Hlth, Li Ka Shing Fac Med, WHO,Collaborating Ctr Infect Dis Epidemiol & Cont, Hong Kong, Peoples R China	Cowling, BJ (reprint author), Univ Hong Kong, Sch Publ Hlth, Li Ka Shing Fac Med, WHO,Collaborating Ctr Infect Dis Epidemiol & Cont, Hong Kong, Peoples R China.	bcowling@hku.hk	Ribeiro, Nuno/AAH-2299-2020; Lau, Eric/C-4487-2009; Wu, Joseph Tsz Kei/C-4450-2009; Leung, Kathy/D-5605-2017	Lau, Eric/0000-0002-6688-9637; Wu, Joseph Tsz Kei/0000-0002-3155-5987; Leung, Kathy/0000-0003-4777-388X	Health and Medical Research Fund, Food and Health Bureau, Government of the Hong Kong Special Administrative Region	This project was supported by a commissioned grant from the Health and Medical Research Fund, Food and Health Bureau, Government of the Hong Kong Special Administrative Region.	Amer H, 2018, AM J INFECT CONTROL, V46, P1022, DOI 10.1016/j.ajic.2018.02.023; [Anonymous], 2020, BEIJING NEWS; Cauchemez S., 2013, Eurosurveillance, V18, P20503; Cauchemez S, 2013, PLOS MED, V10, DOI 10.1371/journal.pmed.1001399; CDC, 2020, 1 TRAV REL CAS 2019; Cheung J., 2020, THE STANDARD    0116; Chinese National Health Commission (NHC), 2020, EXP ANSW REP QUEST P; Cowling BJ, 2015, EUROSURVEILLANCE, V20, P7, DOI 10.2807/1560-7917.ES2015.20.25.21163; Cowling BJ, 2006, ARCH INTERN MED, V166, P1505, DOI 10.1001/archinte.166.14.1505; Fan Y, 2019, VIRUSES-BASEL, V11, DOI 10.3390/v11030210; Ghani AC, 2005, AM J EPIDEMIOL, V162, P479, DOI 10.1093/aje/kwi230; Guan Y, 2004, LANCET, V363, P99, DOI 10.1016/S0140-6736(03)15259-2; Hui DS, 2018, LANCET INFECT DIS, V18, pE217, DOI 10.1016/S1473-3099(18)30127-0; Hui DS, 2017, CLIN CHEST MED, V38, P71, DOI 10.1016/j.ccm.2016.11.007; Jewell NP, 2007, STAT MED, V26, P1982, DOI 10.1002/sim.2691; Kim KH, 2017, J HOSP INFECT, V95, P207, DOI 10.1016/j.jhin.2016.10.008; Kucharski AJ, 2015, EUROSURVEILLANCE, V20, P14, DOI 10.2807/1560-7917.ES2015.20.25.21167; Lau EHY, 2010, BMC INFECT DIS, V10, DOI 10.1186/1471-2334-10-50; Leung GM, 2004, ANN INTERN MED, V141, P662, DOI 10.7326/0003-4819-141-9-200411020-00006; Su S, 2016, TRENDS MICROBIOL, V24, P490, DOI 10.1016/j.tim.2016.03.003; Taiwan Centers for Disease Control (CDC), 2020, TAIW TIM ID 1 IMP CA; WHO, 2019, MERS SIT UPD; Wong G, 2015, CELL HOST MICROBE, V18, P398, DOI 10.1016/j.chom.2015.09.013; World Health Organization, 2020, NOV COR THAIL EXCH; World Health Organization Regional Office for the Western Pacific ( WHO/ Pacific), 2020, WHO W PAC TWITT FEED; World Health Organization ( WHO), 2020, WHO STAT REG CLUST P; Wuhan Municipal Health Commission, 2020, QUEST ANSW PENUM DUE; Wuhan Municipal Health Commission, 2020, REP UN VIR PNEUM; Yu HJ, 2013, LANCET, V382, P138, DOI 10.1016/S0140-6736(13)61207-6	29	5	5	80	80	EUR CENTRE DIS PREVENTION & CONTROL	STOCKHOLM	TOMTEBODAVAGEN 11A, STOCKHOLM, 171 83, SWEDEN	1560-7917			EUROSURVEILLANCE	Eurosurveillance	JAN 23	2020	25	3					4	9		10.2807/1560-7917.ES.2020.25.3.2000044			6	Infectious Diseases	Infectious Diseases	KF6OZ	WOS:000509360400002	31992388	DOAJ Gold, Green Published			2020-04-01	
J	Zheng, YY; Ma, YT; Zhang, JY; Xie, X				Zheng, Ying-Ying; Ma, Yi-Tong; Zhang, Jin-Ying; Xie, Xiang			COVID-19 and the cardiovascular system	NATURE REVIEWS CARDIOLOGY			English	Editorial Material; Early Access								Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infects host cells through ACE2 receptors, leading to coronavirus disease (COVID-19)-related pneumonia, while also causing acute myocardial injury and chronic damage to the cardiovascular system. Therefore, particular attention should be given to cardiovascular protection during treatment for COVID-19.	[Zheng, Ying-Ying; Zhang, Jin-Ying] Zhengzhou Univ, Affiliated Hosp 1, Dept Cardiol, Zhengzhou, Peoples R China; [Zheng, Ying-Ying; Zhang, Jin-Ying] Key Lab Cardiac Injury & Repair Henan Prov, Zhengzhou, Peoples R China; [Ma, Yi-Tong; Xie, Xiang] Xinjiang Med Univ, Affiliated Hosp 1, Dept Cardiol, Urumqi, Peoples R China	Zhang, JY (reprint author), Zhengzhou Univ, Affiliated Hosp 1, Dept Cardiol, Zhengzhou, Peoples R China.; Zhang, JY (reprint author), Key Lab Cardiac Injury & Repair Henan Prov, Zhengzhou, Peoples R China.; Ma, YT; Xie, X (reprint author), Xinjiang Med Univ, Affiliated Hosp 1, Dept Cardiol, Urumqi, Peoples R China.	myt_xj@sina.com; jyzhang@zzu.edu.cn; xiangxie999@sina.com					Alhogbani T, 2016, ANN SAUDI MED, V36, P78, DOI 10.5144/0256-4947.2016.78; Badawi A, 2016, INT J INFECT DIS, V49, P129, DOI 10.1016/j.ijid.2016.06.015; Chan JFW, 2020, LANCET, V395, P514, DOI 10.1016/S0140-6736(20)30154-9; Huang CL, 2020, LANCET, V395, P497, DOI 10.1016/S0140-6736(20)30183-5; Sakabe Masao, 2013, J Cardiol Cases, V8, P173, DOI 10.1016/j.jccase.2013.08.002; Turner AJ, 2004, TRENDS PHARMACOL SCI, V25, P291, DOI 10.1016/j.tips.2004.04.001; Wang Dawei, 2020, JAMA, DOI 10.1001/jama.2020.1585; Wong CK, 2004, CLIN EXP IMMUNOL, V136, P95, DOI 10.1111/j.1365-2249.2004.02415.x; Wu Q, 2017, SCI REP-UK, V7, DOI 10.1038/s41598-017-09536-z; Zhou P, 2020, NATURE, DOI 10.1038/s41586-020-2012-7	10	0	0	79	79	NATURE PUBLISHING GROUP	NEW YORK	75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA	1759-5002	1759-5010		NAT REV CARDIOL	Nat. Rev. Cardiol.												10.1038/s41569-020-0360-5		MAR 2020	2	Cardiac & Cardiovascular Systems	Cardiovascular System & Cardiology	KS3VK	WOS:000518240500001	32139904	Bronze			2020-04-01	
J	Al-Tawfiq, JA; Auwaerter, PG				Al-Tawfiq, J. A.; Auwaerter, P. G.			Healthcare-associated infections: the hallmark of Middle East respiratory syndrome coronavirus with review of the literature	JOURNAL OF HOSPITAL INFECTION			English	Review						Middle East respiratory syndrome coronavirus; MERS; Healthcare-associated outbreaks	MERS-COV OUTBREAK; SAUDI-ARABIA; SOUTH-KOREA; FAMILY CLUSTER; HOSPITAL OUTBREAK; HIGH FATALITY; TRANSMISSION; EPIDEMIOLOGY; PREVENTION; PATIENT	Middle East respiratory syndrome coronavirus (MERS-CoV) is capable of causing acute respiratory illness. Laboratory-confirmed MERS-CoV cases may be asymptomatic, have mild disease, or have a life-threatening infection with a high case fatality rate. There are three patterns of transmission: sporadic community cases from presumed non-human exposure, family clusters arising from contact with an infected family index case, and healthcare-acquired infections among patients and from patients to healthcare workers. Healthcare-acquired MERS infection has become a well-known characteristic of the disease and a leading means of spread. The main factors contributing to healthcare-associated outbreaks include delayed recognition, inadequate infection control measures, inadequate triaging and isolation of suspected MERS or other respiratory illness patients, crowding, and patients remaining in the emergency department for many days. A review of the literature suggests that effective control of hospital outbreaks was accomplished in most instances by the application of proper infection control procedures. Prompt recognition, isolation and management of suspected cases are key factors for prevention of the spread of MERS. Repeated assessments of infection control and monitoring of corrective measures contribute to changing the course of an outbreak. Limiting the number of contacts and hospital visits are also important factors to decrease the spread of infection. (C) 2018 The Healthcare Infection Society. Published by Elsevier Ltd. All rights reserved.	[Al-Tawfiq, J. A.] Johns Hopkins Aramco Healthcare, Specialty Internal Med, Dhahran, Saudi Arabia; [Al-Tawfiq, J. A.] Indiana Univ, Indiana Univ Sch Med, Indianapolis, IN 46204 USA; [Al-Tawfiq, J. A.] Johns Hopkins Univ, Sch Med, Baltimore, MD USA; [Auwaerter, P. G.] Johns Hopkins Univ, Sch Med, Sherrilyn & Ken Fisher Ctr Environm Infect Dis, Baltimore, MD USA	Al-Tawfiq, JA (reprint author), Johns Hopkins Aramco Healthcare, Dhahran Hlth Ctr, POB 76,Room A-428-2,Bldg 61, Dhahran 31311, Saudi Arabia.	jaffar.tawfiq@jhah.com					Abroug F, 2014, EMERG INFECT DIS, V20, P1527, DOI 10.3201/eid2009.140378; Al Hosani FI, 2016, EMERG INFECT DIS, V22, P1162, DOI 10.3201/eid2207.160040; Al-Abdallat MM, 2014, CLIN INFECT DIS, V59, P1225, DOI 10.1093/cid/ciu359; Al-Gethamy M, 2015, CLIN INFECT DIS, V60, P973, DOI 10.1093/cid/ciu1135; Al-Tawfiq JA, 2018, J CHEMOTHERAPY, V30, P179, DOI 10.1080/1120009X.2018.1429236; Al-Tawfiq JA, 2018, J INFECT PUBLIC HEAL, V11, P260, DOI 10.1016/j.jiph.2017.08.006; Al-Tawfiq JA, 2016, EXPERT REV RESP MED, V10, P331, DOI 10.1586/17476348.2016.1150784; Al-Tawfiq JA, 2015, CURR OPIN INFECT DIS, V28, P392, DOI 10.1097/QCO.0000000000000178; Al-Tawfiq JA, 2014, INFECT DRUG RESIST, V7, P281, DOI 10.2147/IDR.S51283; Al-Tawfiq JA, 2014, CLIN INFECT DIS, V59, P160, DOI 10.1093/cid/ciu226; Aldrees T, 2017, SAUDI MED J, V38, P942, DOI 10.15537/smj.2017.9.20626; Alenazi TH, 2017, CLIN INFECT DIS, V65, P675, DOI 10.1093/cid/cix352; Alfaraj SH, 2018, AM J INFECT CONTROL, V46, P165, DOI 10.1016/j.ajic.2017.08.010; Alfaraj SH, 2017, INTERVIROLOGY, V60, P53, DOI 10.1159/000477908; Almekhlafi GA, 2016, CRIT CARE, V20, DOI 10.1186/s13054-016-1303-8; Alraddadi B, 2016, ANN THORAC MED, V11, P128, DOI 10.4103/1817-1737.180027; Aly M, 2017, PLOS ONE, V12, DOI 10.1371/journal.pone.0183850; Assiri A, 2016, EMERG INFECT DIS, V22, P32, DOI 10.3201/eid2201.151370; Assiri A, 2013, LANCET INFECT DIS, V13, P752, DOI 10.1016/S1473-3099(13)70204-4; Assiri A, 2013, NEW ENGL J MED, V369, P407, DOI 10.1056/NEJMoa1306742; Assiri AM, 2016, OPEN FORUM INFECT DI, V3, DOI 10.1093/ofid/ofw165; Balkhy H, 2016, J INFECT PUBLIC HEAL, V9, P1, DOI 10.1016/j.jiph.2015.11.001; Balkhy H, 2013, J INFECT PUBLIC HEAL, V6, P317, DOI 10.1016/j.jiph.2013.06.002; Balkhy HH, 2016, INFECT CONT HOSP EP, V37, P1147, DOI 10.1017/ice.2016.132; Balkhy HH, 2016, MMWR-MORBID MORTAL W, V65, P163, DOI 10.15585/mmwr.mm6506a5; Bawazir A, 2018, J INFECT PUBLIC HEAL, V11, P89, DOI 10.1016/j.jiph.2017.05.003; Bin SY, 2016, CLIN INFECT DIS, V62, P755, DOI 10.1093/cid/civ1020; Cauchemez S., 2013, Eurosurveillance, V18, P20503; Cauchemez S, 2014, LANCET INFECT DIS, V14, P50, DOI 10.1016/S1473-3099(13)70304-9; Centers for Disease Control and Prevention, 2015, INT INF PREV CONTR R; Cha RH, 2016, J KOREAN MED SCI, V31, P635, DOI 10.3346/jkms.2016.31.4.635; Choi Ilsu, 2017, Osong Public Health Res Perspect, V8, P373, DOI 10.24171/j.phrp.2017.8.6.03; Choi S, 2018, J HOSP INFECT, V99, P162, DOI 10.1016/j.jhin.2017.09.017; Chowell G, 2015, BMC MED, V13, DOI 10.1186/s12916-015-0450-0; Command and Control Center Ministry of Health Kingdom of Saudi Arabia Scientific Advisory Board, 2017, INF PREV CONTR GUID; Cowling BJ, 2015, EUROSURVEILLANCE, V20, P7, DOI 10.2807/1560-7917.ES2015.20.25.21163; Drosten C, 2015, CLIN INFECT DIS, V60, P369, DOI 10.1093/cid/ciu812; El Bushra HE, 2016, E MEDITERR HEALTH J, V22, P467; El Bushra HE, 2017, AM J INFECT CONTROL, V45, P502, DOI 10.1016/j.ajic.2016.12.020; Fagbo SF, 2015, EMERG INFECT DIS, V21, P1981, DOI 10.3201/eid2111.150944; Foote MMK, 2017, MMWR-MORBID MORTAL W, V66, P945, DOI 10.15585/mmwr.mm6636a2; Hastings DL, 2016, EMERG INFECT DIS, V22, P794, DOI 10.3201/eid2205.151797; Hijawi B, 2013, E MEDITERR HEALTH J, V19, pS12; Hunter JC, 2016, EMERG INFECT DIS, V22, P647, DOI 10.3201/eid2204.151615; Jeong HW, 1978, OPEN FORUM INFECT S1, V2; Kang CK, 2017, J KOREAN MED SCI, V32, P744, DOI 10.3346/jkms.2017.32.5.744; Ki M, 2015, EPIDEMIOL HLTH, V2015, P37, DOI DOI 10.4178/EPIH/E2015033; Kim KH, 2017, J HOSP INFECT, V95, P207, DOI 10.1016/j.jhin.2016.10.008; Kim SW, 2017, CLIN INFECT DIS, V64, P551, DOI 10.1093/cid/ciw768; Kim SH, 2016, CLIN INFECT DIS, V63, P363, DOI 10.1093/cid/ciw239; Kim Y, 2015, EPIDEMIOL HEALTH, V37, DOI 10.4178/epih/e2015006; Kim Yunhwan, 2016, Osong Public Health Res Perspect, V7, P49, DOI 10.1016/j.phrp.2016.01.001; Koenig KL, 2015, WEST J EMERG MED, V16, P619, DOI 10.5811/westjem.2015.7.27915; Kraaij-Dirkzwager M, 2014, EUROSURVEILLANCE, V19, P2, DOI 10.2807/1560-7917.ES2014.19.21.20817; Lamers MM, 2016, EMERG INFECT DIS, V22, P716, DOI 10.3201/eid2204.152065; Lee J, 2016, INFECT CHEMOTHER, V48, P71, DOI 10.3947/ic.2016.48.2.71; Lee JY, 2018, AM J INFECT CONTROL, V46, P436, DOI 10.1016/j.ajic.2017.09.017; Mailles A, 2013, EUROSURVEILLANCE, V18, P2; Majumder MS, 2017, T ROY SOC TROP MED H, V111, P261, DOI 10.1093/trstmh/trx046; Memish ZA, 2015, TRAVEL MED INFECT DI, V13, P311, DOI 10.1016/j.tmaid.2015.06.012; Memish ZA, 2014, INT J INFECT DIS, V29, P307, DOI 10.1016/j.ijid.2014.10.002; Memish ZA, 2014, J INFECT DIS, V210, P1590, DOI 10.1093/infdis/jiu292; Memish ZA, 2014, INT J INFECT DIS, V23, P63, DOI 10.1016/j.ijid.2014.03.1372; Memish ZA, 2013, NEW ENGL J MED, V368, P2487, DOI 10.1056/NEJMoa1303729; Moher David, 2009, Open Med, V3, pe123; Moon SY, 2017, CLIN INFECT DIS, V64, P1457, DOI 10.1093/cid/cix170; Nam HS, 2017, INT J INFECT DIS, V58, P37, DOI 10.1016/j.ijid.2017.02.008; Nazer RI, 2017, ANN THORAC SURG, V104, pE127, DOI 10.1016/j.athoracsur.2017.02.072; Nishiura H, 2016, BMJ OPEN, V6, DOI 10.1136/bmjopen-2015-009936; Nour MO, 2017, E MEDITERR HEALTH J, V23, P534, DOI 10.26719/2017.23.8.534; Oboho IK, 2015, NEW ENGL J MED, V372, P846, DOI 10.1056/NEJMoa1408636; Oh MD, 2015, J KOREAN MED SCI, V30, P1701, DOI 10.3346/jkms.2015.30.11.1701; Omrani AS, 2013, INT J INFECT DIS, V17, pE668, DOI 10.1016/j.ijid.2013.07.001; Park HY, 2015, EUROSURVEILLANCE, V20, P2, DOI 10.2807/1560-7917.ES2015.20.25.21169; Poissy J, 2014, J CLIN VIROL, V61, P275, DOI 10.1016/j.jcv.2014.07.002; Pyankov OV, 2018, J AEROSOL SCI, V115, P158, DOI 10.1016/j.jaerosci.2017.09.009; Rabaan AA, 2017, J INFECT PUBLIC HEAL, V10, P548, DOI 10.1016/j.jiph.2016.11.008; Saad M, 2014, INT J INFECT DIS, V29, P301, DOI 10.1016/j.ijid.2014.09.003; Saudi Ministry of Health, 2015, MOHS COMM CONTR CTR; van Doremalen N, 2013, EUROSURVEILLANCE, V18, P7, DOI 10.2807/1560-7917.ES2013.18.38.20590; WHO, 2016, MIDDL E RESP SYNDR C; World Health Organization, 2016, WHO UPD CLAR REC MER; World Health Organization, 2015, INF PREV CONTR HLTH; World Health Organization, 2017, MIDDL E RESP SYNDR C; World Health Organization (WHO), 2014, MIDDL E RESP SYNDR C; World Health Organization (WHO), 2015, LAB TEST MIDDL E RES; Zaki AM, 2012, NEW ENGL J MED, V367, P1814, DOI 10.1056/NEJMoa1211721; Zhou F, 2017, SCI ADV, V3, DOI 10.1126/sciadv.1700422	88	12	12	65	78	W B SAUNDERS CO LTD	LONDON	32 JAMESTOWN RD, LONDON NW1 7BY, ENGLAND	0195-6701	1532-2939		J HOSP INFECT	J. Hosp. Infect.	JAN	2019	101	1					20	29		10.1016/j.jhin.2018.05.021			10	Public, Environmental & Occupational Health; Infectious Diseases	Public, Environmental & Occupational Health; Infectious Diseases	HI3VQ	WOS:000456379900004	29864486	Bronze	Y	N	2020-04-01	
J	Vetter, P; Eckerle, I; Kaiser, L				Vetter, Pauline; Eckerle, Isabella; Kaiser, Laurent			Covid-19: a puzzle with many missing pieces	BMJ-BRITISH MEDICAL JOURNAL			English	Editorial Material									[Vetter, Pauline; Eckerle, Isabella; Kaiser, Laurent] Geneva Univ Hosp, Geneva Ctr Emerging Viral Dis, CH-1211 Geneva 14, Switzerland; [Vetter, Pauline; Kaiser, Laurent] Geneva Univ Hosp, Div Infect Dis, Geneva, Switzerland; [Eckerle, Isabella; Kaiser, Laurent] Univ Geneva, Fac Med, CH-1205 Geneva, Switzerland	Vetter, P (reprint author), Geneva Univ Hosp, Geneva Ctr Emerging Viral Dis, CH-1211 Geneva 14, Switzerland.; Vetter, P (reprint author), Geneva Univ Hosp, Div Infect Dis, Geneva, Switzerland.	pauline.vetter@hcuge.ch	Ribeiro, Nuno/AAH-2299-2020				Alansari K, 2019, PEDIATRICS, V143, DOI 10.1542/peds.2018-2308; Beigel JH, 2018, CURR OPIN INFECT DIS, V31, P527, DOI 10.1097/QCO.0000000000000499; Chan JFW, 2020, LANCET, V395, P514, DOI 10.1016/S0140-6736(20)30154-9; Coronavirus disease, 2020, COR DIS 2019 COVID 1; Huang CL, 2020, LANCET, V395, P497, DOI 10.1016/S0140-6736(20)30183-5; Mulangu S, 2019, NEW ENGL J MED, V381, P2293, DOI 10.1056/NEJMoa1910993; Pongpirul WA, 2020, NEW ENGL J MED, DOI [10.1056/NEJMc200162132050060, DOI 10.1056/NEJMC200162132050060]; To KK, 2020, CLIN INFECT DIS, DOI 10.1093/cid/ciaa14932047895; Wang ML, 2020, CELL RES, V30, P269, DOI 10.1038/s41422-020-0282-0; Xu XW, 2020, BMJ-BRIT MED J, V368, DOI 10.1136/bmj.m606; Zhang H, 2020, DIGESTIVE SYSTEM IS, DOI [10.1101/2020.01.30, DOI 10.1101/2020.01.30]; Zhu N, 2020, NEW ENGL J MED, DOI [10.1056/NEJMoa200101731978945, DOI 10.1056/NEJMOA200101731978945]	12	0	0	77	77	BMJ PUBLISHING GROUP	LONDON	BRITISH MED ASSOC HOUSE, TAVISTOCK SQUARE, LONDON WC1H 9JR, ENGLAND	1756-1833			BMJ-BRIT MED J	BMJ-British Medical Journal	FEB 19	2020	368								m627	10.1136/bmj.m627			2	Medicine, General & Internal	General & Internal Medicine	KO0KS	WOS:000515235300011	32075791	Bronze			2020-04-01	
J	Jia, HP				Jia, Hepeng			Diagnosing COVID-19	CHEMICAL & ENGINEERING NEWS			English	News Item																	0	0	0	73	73	AMER CHEMICAL SOC	WASHINGTON	1155 16TH ST, NW, WASHINGTON, DC 20036 USA	0009-2347	1520-605X		CHEM ENG NEWS	Chem. Eng. News	FEB 24	2020	98	8					5	5					1	Chemistry, Multidisciplinary; Engineering, Chemical	Chemistry; Engineering	KQ0XL	WOS:000516654700005					2020-04-01	
J	Catton, H				Catton, H.			Global challenges in health and health care for nurses and midwives everywhere	INTERNATIONAL NURSING REVIEW			English	Article						climate change; coronavirus; COVID-19 recruitment and retention; Year of the Nurse and Midwife		The next decade is likely to produce any number of global challenges that will affect health and health care, including pan-national infections such as the new coronavirus COVID-19 and others that will be related to global warming. Nurses will be required to react to these events, even though they will also be affected as ordinary citizens. The future resilience of healthcare services will depend on having sufficient numbers of nurses who are adequately resourced to face the coming challenges.	[Catton, H.] Int Council Nurses, Geneva, Switzerland	Catton, H (reprint author), Int Council Nurses, Geneva, Switzerland.	catton@icn.ch	Ribeiro, Nuno/AAH-2299-2020				International Council of Nurses, 2018, NURS CLIM CHANG HLTH; International Council of Nurses, 2019, COR COMP DIS NURS VE; International Council of Nurses, 2020, POP ACKN INT YEAR NU; Organisation WH, 2020, 2 M INT HLTH REG 200; Watts N, 2019, LANCET, V394, P1836, DOI 10.1016/S0140-6736(19)32596-6; World Health Organization, 2020, YEAR NURS MIDW 2020	6	0	0	72	72	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0020-8132	1466-7657		INT NURS REV	Int. Nurs. Rev.	MAR	2020	67	1					4	6		10.1111/inr.12578			3	Nursing	Nursing	KN3QG	WOS:000514754800002	32083728	Bronze			2020-04-01	
J	Jin, YB; Long, JT; Ma, X; Zhou, TH; Zhang, ZZ; Lin, HX; Long, JL; Wang, XX				Jin, Yubo; Long, Jintao; Ma, Xi; Zhou, Tanghua; Zhang, Zizhong; Lin, Huaxiang; Long, Jinlin; Wang, Xuxu			Synthesis of caged iodine-modified ZnO nanomaterials and study on their visible light photocatalytic antibacterial properties	APPLIED CATALYSIS B-ENVIRONMENTAL			English	Article						Iodine-modified ZnO; Surface oxygen vacancy defects; Photocatalysis; Disinfection	RESPIRATORY SYNDROME CORONAVIRUS; HIGHLY EFFICIENT; OXYGEN VACANCY; NANOPARTICLES; NANOSHEETS; DESTRUCTION; COMPOSITES; CELLS; FILM	The grain size and surface oxygen vacancy concentration of ZnO have great influence on its photocatalytic performance. In this work, caged Iodine-modified ZnO (I-ZnO-n) catalyst with nano-structure and surface oxygen vacancy were successfully prepared by reflux method. The crystal structure, surface properties and optical properties were characterized by XRD, SEM, TEM, RAMAN, ESR and EPR. And the photocatalytic antibacterial activity under visible light has been tested. The results show that the nano-structure I-ZnO-n's band gap is narrow than ZnO because of the existence of oxygen vacancy. And I-ZnO-n exhibits better antibacterial property than micron-structured Iodine-modified ZnO. The ESR and electrochemical experiments confirm that the I-ZnO-n has much more surface oxygen vacancy and higher separation efficiency of photogenic carriers than micron-structured Iodine-modified ZnO, which results its superior photocatalytic performance.	[Jin, Yubo; Long, Jintao; Ma, Xi; Zhou, Tanghua; Zhang, Zizhong; Lin, Huaxiang; Long, Jinlin; Wang, Xuxu] Fuzhou Univ, Coll Chem, State Key Lab Photocatalysis Energy & Environm, Fuzhou 350116, Fujian, Peoples R China	Lin, HX (reprint author), Fuzhou Univ, Coll Chem, State Key Lab Photocatalysis Energy & Environm, Fuzhou 350116, Fujian, Peoples R China.	lhx@fzu.edu.cn		wang, Xuxu/0000-0003-3228-109X; Zhang, Zizhong/0000-0002-9541-8981	NSFCNational Natural Science Foundation of China [21773031, 21673042, 21673043]; Science and Technology project of Fujian Province of P.R. China [2018H6008]	This work was financially supported by the NSFC (Grant No. 21773031, 21673042 and 21673043), the Science and Technology project of Fujian Province of P.R. China (2018H6008).	Banerjee P, 2012, ULTRASON SONOCHEM, V19, P85, DOI 10.1016/j.ultsonch.2011.05.007; Bykkam S, 2015, SUPERLATTICE MICROST, V83, P776, DOI 10.1016/j.spmi.2015.03.063; Cappitelli F, 2008, APPL ENVIRON MICROB, V74, P564, DOI 10.1128/AEM.01768-07; Chakrabarti S, 2004, J HAZARD MATER, V112, P269, DOI 10.1016/j.jhazmat.2004.05.013; Deng WH, 2016, COLLOID SURFACE B, V144, P196, DOI 10.1016/j.colsurfb.2016.03.085; Etacheri V, 2013, ACS APPL MATER INTER, V5, P1663, DOI 10.1021/am302676a; Gao M., 2018, APPL CATAL B-ENVIRON, V243, P734; Guan Y, 2003, SCIENCE, V302, P276, DOI 10.1126/science.1087139; Guo DD, 2008, J PHOTOCH PHOTOBIO B, V93, P119, DOI 10.1016/j.jphotobiol.2008.07.009; Huang ZA, 2013, ACS APPL MATER INTER, V5, P8663, DOI 10.1021/am4023048; Joanne G., 2010, INT J PHOTOENERGY; Kairyte K, 2013, J PHOTOCH PHOTOBIO B, V128, P78, DOI 10.1016/j.jphotobiol.2013.07.017; Kumar R, 2014, ACS APPL MATER INTER, V6, P13138, DOI 10.1021/am502915v; Lan YQ, 2007, APPL CATAL B-ENVIRON, V73, P354, DOI 10.1016/j.apcatb.2007.01.004; Lee MK, 2016, ANN LAB MED, V36, P154, DOI 10.3343/alm.2016.36.2.154; Lin HX, 2015, APPL CATAL B-ENVIRON, V176, P36, DOI 10.1016/j.apcatb.2015.03.039; Lin QY, 2017, RES CHEM INTERMEDIAT, V43, P5067, DOI 10.1007/s11164-017-3053-x; Liu Q, 2018, CATAL SCI TECHNOL, V8, P4399, DOI 10.1039/c8cy00994e; Liu XW, 2009, COLLOID SURFACE B, V74, P154, DOI 10.1016/j.colsurfb.2009.07.011; MATSUNAGA T, 1985, FEMS MICROBIOL LETT, V29, P211, DOI 10.1111/j.1574-6968.1985.tb00864.x; Moulay S, 2013, J POLYM ENG, V33, P389, DOI 10.1515/polyeng-2012-0122; Murillo G, 2019, NANO ENERGY, V60, P817, DOI 10.1016/j.nanoen.2019.04.017; Nagarajan S, 2008, BMC BIOINFORMATICS, V9, DOI 10.1186/1471-2105-9-168; Ni XS, 2015, MICROBES INFECT, V17, P48, DOI 10.1016/j.micinf.2014.09.007; Ning SB, 2017, APPL CATAL B-ENVIRON, V204, P1, DOI 10.1016/j.apcatb.2016.11.006; Park WB, 2016, J KOREAN MED SCI, V31, P315, DOI 10.3346/jkms.2016.31.2.315; Piva DH, 2017, ADV POWDER TECHNOL, V28, P463, DOI [10.1016/j.apt2016.11.001, 10.1016/j.apt.2016.11.001]; Podporska-Carroll J, 2017, J HAZARD MATER, V324, P39, DOI 10.1016/j.jhazmat.2015.12.038; Prasanna VL, 2015, LANGMUIR, V31, P9155, DOI 10.1021/acs.langmuir.5b02266; Sapkota A, 2011, NANOTECHNOLOGY, V22, DOI 10.1088/0957-4484/22/21/215703; Schumm M, 2007, J PHYS CONF SER, V92, DOI 10.1088/1742-6596/92/1/012149; Simek P, 2015, NANOSCALE, V7, P261, DOI 10.1039/c4nr05219f; SMART R, 1972, J SOC COSMET CHEM, V23, P721; Tan HQ, 2014, ACS APPL MATER INTER, V6, P19184, DOI 10.1021/am5051907; Wang CM, 2014, INTEGR ZOOL, V9, P372, DOI 10.1111/1749-4877.12047; Wang J, 2013, SCI ADV MATER, V5, P1052, DOI 10.1166/sam.2013.1554; Wang WG, 2012, APPL CATAL B-ENVIRON, V119, P109, DOI 10.1016/j.apcatb.2012.02.035; Wang XP, 2013, WATER RES, V47, P4148, DOI 10.1016/j.watres.2012.11.057; Wolcott A, 2009, ADV FUNCT MATER, V19, P1849, DOI 10.1002/adfm.200801363; Wrobel J, 2007, PHYS STATUS SOLIDI B, V244, P1538, DOI 10.1002/pssb.200675132; Wu SH, 2018, SCI REP-UK, V8, DOI 10.1038/s41598-018-26494-2; Xiao GC, 2008, J PHOTOCH PHOTOBIO A, V193, P213, DOI 10.1016/j.jphotochem.2007.06.027; Xiao LH, 2005, CATAL COMMUN, V6, P796, DOI 10.1016/j.catcom.2005.07.015; Xiao Q, 2008, J HAZARD MATER, V150, P62, DOI 10.1016/j.jhazmat.2007.04.045; Yang RW, 2017, APPL CATAL B-ENVIRON, V210, P184, DOI 10.1016/j.apcatb.2017.03.064; Ye LQ, 2012, PHYS CHEM CHEM PHYS, V14, P82, DOI 10.1039/c1cp22876e; Zhang YQ, 2018, APPL CATAL B-ENVIRON, V235, P238, DOI 10.1016/j.apcatb.2018.05.001; Zhang ZB, 1998, J PHYS CHEM B, V102, P10871, DOI 10.1021/jp982948+	48	4	4	67	72	ELSEVIER	AMSTERDAM	RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS	0926-3373	1873-3883		APPL CATAL B-ENVIRON	Appl. Catal. B-Environ.	NOV 5	2019	256								UNSP 117873	10.1016/j.apcatb.2019.117873			8	Chemistry, Physical; Engineering, Environmental; Engineering, Chemical	Chemistry; Engineering	IU3AN	WOS:000483451200080					2020-04-01	
J	Velavan, TP; Meyer, CG				Velavan, Thirumalaisamy P.; Meyer, Christian G.			The COVID-19 epidemic	TROPICAL MEDICINE & INTERNATIONAL HEALTH			English	Editorial Material						COVID-19; Epidemic; SARS-CoV2; Wuhan; 2019-nCoV	SARS		[Velavan, Thirumalaisamy P.; Meyer, Christian G.] Univ Klinikum Tubingen, Inst Trop Med, Tubingen, Germany; [Velavan, Thirumalaisamy P.; Meyer, Christian G.] Vietnamese German Ctr Med Res, Hanoi, Vietnam; [Velavan, Thirumalaisamy P.; Meyer, Christian G.] Duy Tan Univ, Fac Med, Da Nang, Viet Nam	Velavan, TP (reprint author), Inst Trop Med, Wilhelmstr 27, D-72074 Tubingen, Germany.	velavan@medizin.uni-tuebingen.de		Velavan, Thirumalaisamy P/0000-0002-9809-9883			Bauch CT, 2005, EPIDEMIOLOGY, V16, P791, DOI 10.1097/01.ede.0000181633.80269.4c; Chan JFW, 2020, LANCET, V395, P514, DOI 10.1016/S0140-6736(20)30154-9; Chu CM, 2004, THORAX, V59, P252, DOI 10.1136/thorax.2003.012658; GISAID Global Initiative on Sharing All Influenza Data, PHYL SARS BET INCL N; Guan W, 2020, CLIN CHARACTERISTICS, DOI [10.1101/2020.02.06.20020974, DOI 10.1101/2020.02.06.20020974]; Holshue ML, 2019, N ENGL J MED; Kono M, 2008, ANTIVIR RES, V77, P150, DOI 10.1016/j.antiviral.2007.10.011; Li Qun, 2020, N Engl J Med, V382, P1199, DOI 10.1056/NEJMoa2001316; Richardson P, 2020, LANCET, V395, pE30, DOI 10.1016/S0140-6736(20)30304-4; Rottier PJM., 2013, CORONAVIRIDAE, P115; TYRRELL DA, 1966, LANCET, V1, P76; Wang ML, 2020, CELL RES, V30, P269, DOI 10.1038/s41422-020-0282-0; World Health Organization, 2019, MIDDL E RESP SYNDR C; World Health Organization, 2020, CUM NUMB REP PROBL C, DOI DOI 10.1016/J.IJID.2020.01.050; Zhao Shi, 2020, Int J Infect Dis, V92, P214, DOI 10.1016/j.ijid.2020.01.050; Zhou P, 2020, NATURE, DOI 10.1038/s41586-020-2012-7	16	0	0	71	71	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	1360-2276	1365-3156		TROP MED INT HEALTH	Trop. Med. Int. Health	MAR	2020	25	3					278	280		10.1111/tmi.13383			3	Public, Environmental & Occupational Health; Tropical Medicine	Public, Environmental & Occupational Health; Tropical Medicine	KR1QV	WOS:000517394200001	32052514	Bronze			2020-04-01	
J	MacKenzie, D				MacKenzie, Debora			Covid-19 goes global	NEW SCIENTIST			English	News Item																	0	0	0	71	71	REED BUSINESS INFORMATION LTD	SUTTON	QUADRANT HOUSE THE QUADRANT, SUTTON SM2 5AS, SURREY, ENGLAND	0262-4079			NEW SCI	New Sci.	FEB 29	2020	245	3271					7	7					1	Multidisciplinary Sciences	Science & Technology - Other Topics	KR5NS	WOS:000517665700001					2020-04-01	
J	Lim, J; Jeon, S; Shin, HY; Kim, MJ; Seong, YM; Lee, WJ; Choe, KW; Kang, YM; Lee, B; Park, SJ				Lim, Jaegyun; Jeon, Seunghyun; Shin, Hyun-Young; Kim, Moon Jung; Seong, Yu Min; Lee, Wang Jun; Choe, Kang-Won; Kang, Yu Min; Lee, Baeckseung; Park, Sang-Joon			Case of the Index Patient Who Caused Tertiary Transmission of Coronavirus Disease 2019 in Korea: the Application of Lopinavir/Ritonavir for the Treatment of COVID-19 Pneumonia Monitored by Quantitative RT-PCR	JOURNAL OF KOREAN MEDICAL SCIENCE			English	Article						Coronavirus; COVID-19; Pneumonia; Tertiary Infection; Viral Load; Real Time Reverse-Transcriptase Polymerase Chain Reaction		Since mid-December of 2019, coronavirus disease 2019 (COVID-19) has been spreading from Wuhan, China. The confirmed COVID-19 patients in South Korea are those who came from or visited China. As secondary transmissions have occurred and the speed of transmission is accelerating, there are rising concerns about community infections. The 54-year old male is the third patient diagnosed with COVID-19 in Korea. He is a worker for a clothing business and had mild respiratory symptoms and intermittent fever in the beginning of hospitalization, and pneumonia symptoms on chest computerized tomography scan on day 6 of admission. This patient caused one case of secondary transmission and three cases of tertiary transmission. Hereby, we report the clinical findings of the index patient who was the first to cause tertiary transmission outside China. Interestingly, after lopinavir/ritonavir (Kaletra, AbbVie) was administered, beta-coronavirus viral loads significantly decreased and no or little coronavirus titers were observed.	[Lim, Jaegyun; Kim, Moon Jung] Hanyang Univ, Myongji Hosp, Coll Med, Dept Lab Med, Goyang, South Korea; [Jeon, Seunghyun] Myongji Hosp, New Horizon Canc Inst, Goyang, South Korea; [Shin, Hyun-Young] Hanyang Univ, Myongji Hosp, Coll Med, Dept Family Med, Goyang, South Korea; [Seong, Yu Min] Myongji Hosp, Dept Internal Med, Goyang, South Korea; [Lee, Wang Jun] Myongji Hosp, Dept Gen Surg, Goyang, South Korea; [Choe, Kang-Won; Kang, Yu Min] Myongji Hosp, Dept Infect Dis, Goyang, South Korea; [Lee, Baeckseung] CancerROP, 173 Digital Ro, Seoul 08511, South Korea; [Park, Sang-Joon] Myongji Hosp, Dept Pulm & Crit Care Med, 55 Havasu Ro,14 Beon Gil, Goyang 10475, South Korea	Lee, B (reprint author), CancerROP, 173 Digital Ro, Seoul 08511, South Korea.; Park, SJ (reprint author), Myongji Hosp, Dept Pulm & Crit Care Med, 55 Havasu Ro,14 Beon Gil, Goyang 10475, South Korea.	baeckseung@gmail.com; drjoseph@mjh.or.kr	Ribeiro, Nuno/AAH-2299-2020	Kim, Moon Jung/0000-0003-4148-9116; Kang, Yu Min/0000-0002-4368-9878; Lim, Jaegyun/0000-0002-3553-0058; Shin, Hyun-Young/0000-0001-7261-3365			Chang D, 2020, JAMA; Hamer M, 2019, BRAIN BEHAV IMMUN, V76, P280, DOI 10.1016/j.bbi.2018.12.011; Kim JY, 2020, J KOREAN MED SCI, V35, DOI 10.3346/jkms.2020.35.e61; Li Q, 2020, N ENGL J MED; Rothe C, 2020, N ENGL J MED	5	3	3	71	71	KOREAN ACAD MEDICAL SCIENCES	SEOUL	302 75 DONG DU ICHON, DONG YONGSAN KU, SEOUL 140 031, SOUTH KOREA	1011-8934	1598-6357		J KOREAN MED SCI	J. Korean Med. Sci.	FEB 17	2020	35	6							e79	10.3346/jkms.2020.35.e79			6	Medicine, General & Internal	General & Internal Medicine	KN4NN	WOS:000514815500010	32056407	DOAJ Gold, Green Published			2020-04-01	
J	Park, M; Thwaites, RS; Openshaw, PJM				Park, Mirae; Thwaites, Ryan S.; Openshaw, Peter J. M.			COVID-19: Lessons from SARS and MERS	EUROPEAN JOURNAL OF IMMUNOLOGY			English	Editorial Material									[Park, Mirae; Thwaites, Ryan S.; Openshaw, Peter J. M.] Imperial Coll London, Natl Heart & Lung Inst, London, England	Openshaw, PJM (reprint author), Imperial Coll London, Natl Heart & Lung Inst, London, England.	p.openshaw@imperial.ac.uk					Arabi YM, 2018, AM J RESP CRIT CARE, V197, P757, DOI 10.1164/rccm.201706-1172OC; Chan JFW, 2020, LANCET, V395, P514, DOI 10.1016/S0140-6736(20)30154-9; Chen NS, 2020, LANCET, V395, P507, DOI 10.1016/S0140-6736(20)30211-7; Chinese Centre for Disease Control, 2020, EP CHAR OUTBR 2019 N; Dunning J, 2018, NAT IMMUNOL, V19, P625, DOI 10.1038/s41590-018-0111-5; European Centre for Disease Prevention and Control, COVID 19 SIT UPD EU; Guo LX, 2019, FRONT MICROBIOL, V10, DOI 10.3389/fmicb.2019.02752; Hayward AC, 2014, LANCET RESP MED, V2, P445, DOI 10.1016/S2213-2600(14)70034-7; Huang CL, 2020, LANCET, V395, P497, DOI 10.1016/S0140-6736(20)30183-5; Huang KJ, 2005, J MED VIROL, V75, P185, DOI 10.1002/jmv.20255; Lu RJ, 2020, LANCET, V395, P565, DOI 10.1016/S0140-6736(20)30251-8; Public Health England, 2020, COVID 19 BACKGR INF; Public Health England, 2020, COVID 19 INV IN CLIN; Russell CD, 2020, LANCET, V395, P473, DOI 10.1016/S0140-6736(20)30317-2; Shen L, 2020, J MED ECON, DOI 10.1080/13696998.2020.1717500; Stockman LJ, 2006, PLOS MED, V3, P1525, DOI 10.1371/journal.pmed.0030343; Wang ML, 2020, CELL RES, V30, P269, DOI 10.1038/s41422-020-0282-0; Wei Min, 2020, JAMA, DOI 10.1001/jama.2020.2131; World Health Organisation, 2020, 35 WHO; World Health Organisation, 2003, CONS DOC EP SEV AC R; World Health Organization, 2020, CLIN MAN SEV AC RESP; World Health Organization (WHO), 2020, MIDDL E RESP SYNDR C; World Health Organization ( WHO), 2020, GLOB SURV HUM INF CO	23	0	0	70	70	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0014-2980	1521-4141		EUR J IMMUNOL	Eur. J. Immunol.	MAR	2020	50	3					308	311		10.1002/eji.202070035		FEB 2020	4	Immunology	Immunology	KR5NB	WOS:000516609000001	32104909	Bronze			2020-04-01	
J	Wang, C; Horby, PW; Hayden, FG; Gao, GF				Wang, Chen; Horby, Peter W.; Hayden, Frederick G.; Gao, George F.			A novel coronavirus outbreak of global health concern	LANCET			English	Editorial Material							ACUTE RESPIRATORY SYNDROME; EPIDEMIOLOGY		[Wang, Chen] China Japan Friendship Hosp, Ctr Resp Med, Dept Pulm & Crit Care Med, Beijing 100029, Peoples R China; [Wang, Chen] Natl Clin Res Ctr Resp Dis, Beijing, Peoples R China; [Wang, Chen] Chinese Acad Med Sci & Peking Union Med Coll, Beijing, Peoples R China; [Wang, Chen] Chinese Acad Med Sci, Inst Resp Med, Beijing, Peoples R China; [Wang, Chen] Capital Med Univ, Dept Resp Med, Beijing, Peoples R China; [Horby, Peter W.] Univ Oxford, Ctr Trop Med & Global Hlth, Oxford, England; [Hayden, Frederick G.] Univ Virginia, Sch Med, Dept Med, Charlottesville, VA 22908 USA; [Gao, George F.] Chinese Ctr Dis Control & Prevent China CDC, Natl Inst Viral Dis Control & Prevent, Beijing, Peoples R China	Wang, C (reprint author), China Japan Friendship Hosp, Ctr Resp Med, Dept Pulm & Crit Care Med, Beijing 100029, Peoples R China.; Wang, C (reprint author), Natl Clin Res Ctr Resp Dis, Beijing, Peoples R China.; Wang, C (reprint author), Chinese Acad Med Sci & Peking Union Med Coll, Beijing, Peoples R China.; Wang, C (reprint author), Chinese Acad Med Sci, Inst Resp Med, Beijing, Peoples R China.; Wang, C (reprint author), Capital Med Univ, Dept Resp Med, Beijing, Peoples R China.	cyh-birm@263.net	Ribeiro, Nuno/AAH-2299-2020				[Anonymous], 2020, ANN HEADQ NOV COR PN; Assiri A, 2013, LANCET INFECT DIS, V13, P752, DOI 10.1016/S1473-3099(13)70204-4; Chan JFW, 2020, LANCET, V395, P514, DOI 10.1016/S0140-6736(20)30154-9; China National Health Commission, 2020, UPD NOV COR PNEUM OU; Huang CL, 2020, LANCET, V395, P497, DOI 10.1016/S0140-6736(20)30183-5; Leung GM, 2004, ANN INTERN MED, V141, P662, DOI 10.7326/0003-4819-141-9-200411020-00006; US Centers for Disease Control and Prevention, 2020, 1 TRAV REL CAS 2019; Viboud C, 2013, J INFECT DIS, V207, P721, DOI 10.1093/infdis/jis745; *WHO, 2003, SUMM PROB SARS CAS O; WHO, 2020, 2 WHO; World Health Organization, 2020, NOV COR THAIL EXCH; World Health Organization, 2020, NOV COR JAP EXCH; World Health Organization, 2020, NOV COR REP KOR EXCH; World Health Organization, 2020, CLIN MAN SEV AC RESP; World Health Organization (WHO), 2020, MIDDL E RESP SYNDR C; Zhong NS, 2003, LANCET, V362, P1353, DOI 10.1016/S0140-6736(03)14630-2	16	25	26	69	69	ELSEVIER SCIENCE INC	NEW YORK	STE 800, 230 PARK AVE, NEW YORK, NY 10169 USA	0140-6736	1474-547X		LANCET	Lancet	FEB 15	2020	395	10223					470	473		10.1016/S0140-6736(20)30185-9			5	Medicine, General & Internal	General & Internal Medicine	KN1CG	WOS:000514576900009	31986257	Bronze			2020-04-01	
J	Brown, AJ; Won, JJ; Graham, RL; Dinnon, KH; Sims, AC; Feng, JY; Cihlar, T; Denison, MR; Baric, RS; Sheahan, TP				Brown, Ariane J.; Won, John J.; Graham, Rachel L.; Dinnon, Kenneth H., III; Sims, Amy C.; Feng, Joy Y.; Cihlar, Tomas; Denison, Mark R.; Baric, Ralph S.; Sheahan, Timothy P.			Broad spectrum antiviral remdesivir inhibits human endemic and zoonotic deltacoronaviruses with a highly divergent RNA dependent RNA polymerase	ANTIVIRAL RESEARCH			English	Article						Coronavirus; Emerging viruses; Broad-spectrum antivirals; GS-5743; Remdesivir	HUMAN CORONAVIRUS OC43; PORCINE DELTACORONAVIRUS; SARS; REPLICATION; DISCOVERY; INFECTION; EFFICACY; GS-5734; VIRUS; MODEL	The genetically diverse Orthocoronavirinae (CoV) family is prone to cross species transmission and disease emergence in both humans and livestock. Viruses similar to known epidemic strains circulating in wild and domestic animals further increase the probability of emergence in the future. Currently, there are no approved therapeutics for any human CoV presenting a clear unmet medical need. Remdesivir (RDV, GS-5734) is a monophosphoramidate prodrug of an adenosine analog with potent activity against an array of RNA virus families including Filoviridae, Paramyxoviridae, Pneumoviridae, and Orthocoronavirinae, through the targeting of the viral RNA dependent RNA polymerase (RdRp). We developed multiple assays to further define the breadth of RDV antiviral activity against the CoV family. Here, we show potent antiviral activity of RDV against endemic human CoVs OC43 (HCoV-OC43) and 229E (HCoV-229E) with submicromolar EC50 values. Of known CoVs, the members of the deltacoronavirus genus have the most divergent RdRp as compared to SARS- and MERS-CoV and both avian and porcine members harbor a native residue in the RdRp that confers resistance in beta-CoVs. Nevertheless, RDV is highly efficacious against porcine deltacoronavirus (PDCoV). These data further extend the known breadth and antiviral activity of RDV to include both contemporary human and highly divergent zoonotic CoV and potentially enhance our ability to fight future emerging CoV.	[Brown, Ariane J.; Won, John J.; Graham, Rachel L.; Dinnon, Kenneth H., III; Sims, Amy C.; Baric, Ralph S.; Sheahan, Timothy P.] Univ N Carolina, Dept Epidemiol, Chapel Hill, NC 27515 USA; [Feng, Joy Y.; Cihlar, Tomas] Gilead Sci Inc, 353 Lakeside Dr, Foster City, CA 94404 USA; [Denison, Mark R.] Vanderbilt Univ, Med Ctr, Dept Pathol Microbiol & Immunol, Dept Pediat Infect Dis, Nashville, TN USA	Sheahan, TP (reprint author), Univ N Carolina, Gillings Sch Global Publ Hlth, Dept Epidemiol, 135 Dauer Dr, Chapel Hill, NC 27599 USA.	sheahan@email.unc.edu	Sheahan, Timothy/J-8201-2019	Sheahan, Timothy/0000-0001-9181-2183	Antiviral Drug Discovery and Development Center [5U19AI109680]; National Institutes of Health (NIH), United StatesUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [5R01AI132178]; NIH National Institute of Allergy and Infectious Diseases virology training grant [T32 AI007419]	We would like to acknowledge the following funding sources, Antiviral Drug Discovery and Development Center (5U19AI109680) and a partnership grant from the National Institutes of Health (NIH), United States (5R01AI132178). KD was supported by a fellowship from the NIH National Institute of Allergy and Infectious Diseases virology training grant (T32 AI007419).	Agostini ML, 2018, MBIO, V9, DOI 10.1128/mBio.00221-18; Barnard Dale L., 2006, Antiviral Chemistry & Chemotherapy, V17, P275; Beall A, 2016, MBIO, V7, DOI 10.1128/mBio.01451-15; Carman K.B., 2018, HUM VACC IMMUNOTHER, P1; Chan JFW, 2013, J INFECTION, V67, P606, DOI 10.1016/j.jinf.2013.09.029; de Wilde AH, 2014, ANTIMICROB AGENTS CH, V58, P4875, DOI 10.1128/AAC.03011-14; de Wit E, 2016, NAT REV MICROBIOL, V14, P523, DOI 10.1038/nrmicro.2016.81; Dijkman R, 2012, J CLIN VIROL, V53, P135, DOI 10.1016/j.jcv.2011.11.011; Falsey AR, 2002, J INFECT DIS, V185, P1338, DOI 10.1086/339881; Heimdal I, 2019, J INFECT DIS, V219, P1198, DOI 10.1093/infdis/jiy646; Hu B, 2015, VIROL J, V12, DOI 10.1186/s12985-015-0422-1; Hu H, 2015, J CLIN MICROBIOL, V53, P1537, DOI 10.1128/JCM.00031-15; Huang YW, 2013, MBIO, V4, DOI 10.1128/mBio.00737-13; Huynh J, 2012, J VIROL, V86, P12816, DOI 10.1128/JVI.00906-12; Ibarra KD, 2009, J VIROL, V83, P4538, DOI 10.1128/JVI.02280-08; Jordan PC, 2018, PLOS PATHOG, V14, DOI 10.1371/journal.ppat.1006889; Jung K, 2015, EMERG INFECT DIS, V21, P650, DOI 10.3201/eid2104.141859; Kirchdoerfer RN, 2019, NAT COMMUN, V10, DOI 10.1038/s41467-019-10280-3; Lassnig C, 2005, P NATL ACAD SCI USA, V102, P8275, DOI 10.1073/pnas.0408589102; Lau SKP, 2010, J VIROL, V84, P11385, DOI 10.1128/JVI.01121-10; Leung S, 2001, CAN J PHYSIOL PHARM, V79, P59, DOI 10.1139/cjpp-79-1-59; Lo MK, 2017, SCI REP-UK, V7, DOI 10.1038/srep43395; Loens K, 2012, J CLIN MICROBIOL, V50, P977, DOI 10.1128/JCM.00200-11; Matsuda M, 2018, HEPATOLOGY, V67, P296, DOI 10.1002/hep.29421; Menachery VD, 2016, P NATL ACAD SCI USA, V113, P3048, DOI 10.1073/pnas.1517719113; Murphy BG, 2018, VET MICROBIOL, V219, P226, DOI 10.1016/j.vetmic.2018.04.026; Neuman BW, 2008, J VIROL, V82, P5279, DOI 10.1128/JVI.02631-07; Patrick David M, 2006, Can J Infect Dis Med Microbiol, V17, P330; Sheahan TP, 2017, SCI TRANSL MED, V9, DOI 10.1126/scitranslmed.aal3653; Shen L, 2016, ANTIMICROB AGENTS CH, V60, P5492, DOI 10.1128/AAC.00814-16; Siegel D, 2017, J MED CHEM, V60, P1648, DOI 10.1021/acs.jmedchem.6b01594; St-Jean JR, 2004, J VIROL, V78, P8824, DOI 10.1128/JVI.78.16.8824-8834.2004; Tchesnokov EP, 2019, VIRUSES-BASEL, V11, DOI 10.3390/v11040326; Tchesnokov EP, 2018, SCI REP-UK, V8, DOI 10.1038/s41598-018-22328-3; Varghese L, 2018, J PEDIAT INF DIS SOC, V7, P151, DOI 10.1093/jpids/pix027; Vassilara F, 2018, CASE REP INFECT DIS, DOI 10.1155/2018/6796839; Vijgen L, 2005, J VIROL, V79, P1595, DOI 10.1128/JVI.79.3.1595-1604.2005; Walsh EE, 2013, J INFECT DIS, V208, P1634, DOI 10.1093/infdis/jit393; Warren TK, 2016, NATURE, V531, P381, DOI 10.1038/nature17180; Xu X, 2003, NUCLEIC ACIDS RES, V31, P7117, DOI 10.1093/nar/gkg916; Zhou P, 2018, NATURE, V556, P255, DOI 10.1038/s41586-018-0010-9; Zumla A, 2016, NAT REV DRUG DISCOV, V15, P327, DOI 10.1038/nrd.2015.37	42	0	0	67	68	ELSEVIER	AMSTERDAM	RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS	0166-3542	1872-9096		ANTIVIR RES	Antiviral Res.	SEP	2019	169								UNSP 104541	10.1016/j.antiviral.2019.104541			10	Pharmacology & Pharmacy; Virology	Pharmacology & Pharmacy; Virology	JR9KI	WOS:000499934800006	31233808	Green Published, Bronze			2020-04-01	
J	Xu, X; Yu, CC; Qu, J; Zhang, LG; Jiang, SF; Huang, DY; Chen, BH; Zhang, ZP; Guan, WH; Ling, ZK; Jiang, R; Hu, TL; Ding, Y; Lin, L; Gan, QX; Luo, LP; Tang, XP; Liu, JX				Xu, Xi; Yu, Chengcheng; Qu, Jing; Zhang, Lieguang; Jiang, Songfeng; Huang, Deyang; Chen, Bihua; Zhang, Zhiping; Guan, Wanhua; Ling, Zhoukun; Jiang, Rui; Hu, Tianli; Ding, Yan; Lin, Lin; Gan, Qingxin; Luo, Liangping; Tang, Xiaoping; Liu, Jinxin			Imaging and clinical features of patients with 2019 novel coronavirus SARS-CoV-2	EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING			English	Article; Early Access						2019 novel coronavirus pneumonia; COVID-19; SARS-CoV-2; Infection; Imaging features; Computed tomography; Ground glass opacification	PNEUMONIA	Background The pneumonia caused by the 2019 novel coronavirus (SARS-CoV-2, also called 2019-nCoV) recently break out in Wuhan, China, and was named as COVID-19. With the spread of the disease, similar cases have also been confirmed in other regions of China. We aimed to report the imaging and clinical characteristics of these patients infected with SARS-CoV-2 in Guangzhou, China. Methods All patients with laboratory-identified SARS-CoV-2 infection by real-time polymerase chain reaction (PCR) were collected between January 23, 2020, and February 4, 2020, in a designated hospital (Guangzhou Eighth People's Hospital). This analysis included 90 patients (39 men and 51 women; median age, 50 years (age range, 18-86 years). All the included SARS-CoV-2-infected patients underwent non-contrast enhanced chest computed tomography (CT). We analyzed the clinical characteristics of the patients, as well as the distribution characteristics, pattern, morphology, and accompanying manifestations of lung lesions. In addition, after 1-6 days (mean 3.5 days), follow-up chest CT images were evaluated to assess radiological evolution. Findings The majority of infected patients had a history of exposure in Wuhan or to infected patients and mostly presented with fever and cough. More than half of the patients presented bilateral, multifocal lung lesions, with peripheral distribution, and 53 (59%) patients had more than two lobes involved. Of all included patients, COVID-19 pneumonia presented with ground glass opacities in 65 (72%), consolidation in 12 (13%), crazy paving pattern in 11 (12%), interlobular thickening in 33 (37%), adjacent pleura thickening in 50 (56%), and linear opacities combined in 55 (61%). Pleural effusion, pericardial effusion, and lymphadenopathy were uncommon findings. In addition, baseline chest CT did not show any abnormalities in 21 patients (23%), but 3 patients presented bilateral ground glass opacities on the second CT after 3-4 days. Conclusion SARS-CoV-2 infection can be confirmed based on the patient's history, clinical manifestations, imaging characteristics, and laboratory tests. Chest CT examination plays an important role in the initial diagnosis of the novel coronavirus pneumonia. Multiple patchy ground glass opacities in bilateral multiple lobular with periphery distribution are typical chest CT imaging features of the COVID-19 pneumonia.	[Xu, Xi; Luo, Liangping] Jinan Univ, Dept Med Imaging Ctr, Affiliated Hosp 1, 613,Huangpu Rd West, Guangzhou 510630, Guangdong, Peoples R China; [Yu, Chengcheng; Qu, Jing; Zhang, Lieguang; Jiang, Songfeng; Huang, Deyang; Chen, Bihua; Zhang, Zhiping; Guan, Wanhua; Ling, Zhoukun; Jiang, Rui; Hu, Tianli; Ding, Yan; Lin, Lin; Gan, Qingxin; Tang, Xiaoping; Liu, Jinxin] Guangzhou Med Univ, Guangzhou Peoples Hosp 8, Dept Radiol, 8,Huaying Rd, Guangzhou 510060, Guangdong, Peoples R China	Luo, LP (reprint author), Jinan Univ, Dept Med Imaging Ctr, Affiliated Hosp 1, 613,Huangpu Rd West, Guangzhou 510630, Guangdong, Peoples R China.; Tang, XP; Liu, JX (reprint author), Guangzhou Med Univ, Guangzhou Peoples Hosp 8, Dept Radiol, 8,Huaying Rd, Guangzhou 510060, Guangdong, Peoples R China.	tluolp@jnu.edu.cn; xtang@21cn.com; Liujx83710378@126.com					Chan JFW, 2020, LANCET, V395, P514, DOI 10.1016/S0140-6736(20)30154-9; Chen NS, 2020, LANCET, V395, P507, DOI 10.1016/S0140-6736(20)30211-7; Chong S, 2010, BRIT J RADIOL, V83, P585, DOI 10.1259/bjr/51409455; Chung M, 2020, RADIOLOGY, V295, P202, DOI 10.1148/radiol.2020200230; Cui J, 2019, NAT REV MICROBIOL, V17, P181, DOI 10.1038/s41579-018-0118-9; Das KM, 2016, AM J ROENTGENOL, V206, P1193, DOI 10.2214/AJR.15.15363; Hansell DM, 2008, RADIOLOGY, V246, P697, DOI 10.1148/radiol.2462070712; Heymann DL, 2020, LANCET, V395, P469, DOI 10.1016/S0140-6736(20)30184-7; Huang CL, 2020, LANCET, V395, P497, DOI 10.1016/S0140-6736(20)30183-5; Koo HJ, 2018, RADIOGRAPHICS, V38, P719, DOI 10.1148/rg.2018170048; Ooi GC, 2003, RESPIROLOGY, V8, pS15, DOI 10.1046/j.1440-1843.2003.00519.x; Richman DD, 2016, CLIN VIROLOGY; Su S, 2016, TRENDS MICROBIOL, V24, P490, DOI 10.1016/j.tim.2016.03.003; Xu XT, 2020, SCI CHINA LIFE SCI, V63, P457, DOI 10.1007/s11427-020-1637-5; Zhu N, 2020, NEW ENGL J MED, V382, P727, DOI 10.1056/NEJMoa2001017	15	0	0	65	65	SPRINGER	NEW YORK	ONE NEW YORK PLAZA, SUITE 4600, NEW YORK, NY, UNITED STATES	1619-7070	1619-7089		EUR J NUCL MED MOL I	Eur. J. Nucl. Med. Mol. Imaging												10.1007/s00259-020-04735-9		FEB 2020	6	Radiology, Nuclear Medicine & Medical Imaging	Radiology, Nuclear Medicine & Medical Imaging	KQ4YX	WOS:000516931000001	32107577	Bronze			2020-04-01	
J	Yoshizawa, S; Hattori, Y; Kobayashi, K; Akaji, K				Yoshizawa, Shin-ichiro; Hattori, Yasunao; Kobayashi, Kazuya; Akaji, Kenichi			Evaluation of an octahydroisochromene scaffold used as a novel SARS 3CL protease inhibitor	BIOORGANIC & MEDICINAL CHEMISTRY			English	Article						SARS 3CL protease; Inhibitor; Fused ring scaffold; Octahydroisochromene	ACUTE RESPIRATORY SYNDROME; CORONAVIRUS; DIHYDROXYLATION	An octahydroisochromene scaffold has been introduced into a known SARS 3CL protease inhibitor as a novel hydrophobic core to interact with the S2 pocket of the protease. An alkyl or aryl substituent was also introduced at the 1-position of the octahydroisochromene scaffold and expected to introduce additional interactions with the protease. Sharpless-Katsuki asymmetric epoxidation and Sharpless asymmetric dihydroxylation were employed to construct the octahydroisochromene scaffold. The introductions of the P1 site His-al and the substituent at 1-position was achieved using successive reductive amination reactions. Our initial evaluations of the diastereo-isomeric mixtures (16a-d) revealed that the octahydroisochromene moiety functions as a core hydrophobic scaffold for the S2 pocket of the protease and the substituent at the 1-position may form additional interactions with the protease. The inhibitory activities of the diastereoisomerically-pure inhibitors (3a-d) strongly suggest that a specific stereo-isomer of the octahydroisochromene scaffold, (1S, 3S) 3b, directs the P1 site imidazole, the warhead aldehyde, and substituent at the 1-position of the fused ring to their appropriate pockets in the protease.	[Yoshizawa, Shin-ichiro; Kobayashi, Kazuya; Akaji, Kenichi] Kyoto Pharmaceut Univ, Dept Med Chem, Yamashina Ku, Kyoto 6078412, Japan; [Hattori, Yasunao] Kyoto Pharmaceut Univ, Ctr Instrumental Anal, Yamashina Ku, Kyoto 6078412, Japan	Akaji, K (reprint author), Kyoto Pharmaceut Univ, Dept Med Chem, Kyoto 6078412, Japan.	akaji@mb.kyoto-phu.ac.jp			Japan Society for the Promotion of ScienceMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT)Japan Society for the Promotion of Science [16H05104]	This work was supported, in part, by a Grant-in-aid for Scientific Research 16H05104 given to KA from the Japan Society for the Promotion of Science.	Akaji K., 2018, AMINO ACIDS PEPT PRO, V42, P228; Akaji K, 2008, BIOORGAN MED CHEM, V16, P9400, DOI 10.1016/j.bmc.2008.09.057; Akaji K, 2011, J MED CHEM, V54, P7962, DOI 10.1021/jm200870n; BASHA A, 1977, TETRAHEDRON LETT, P4171; Bredikhina ZA, 2014, TETRAHEDRON-ASYMMETR, V25, P1015, DOI 10.1016/j.tetasy.2014.05.008; Corey EJ, 2001, ORG LETT, V3, P3211, DOI 10.1021/ol016577i; Drosten C, 2003, NEW ENGL J MED, V348, P1967, DOI 10.1056/NEJMoa030747; HENTGES SG, 1980, J AM CHEM SOC, V102, P4263, DOI 10.1021/ja00532a050; KATSUKI T, 1980, J AM CHEM SOC, V102, P5974, DOI 10.1021/ja00538a077; Ksiazek TG, 2003, NEW ENGL J MED, V348, P1953, DOI 10.1056/NEJMoa030781; Lee N, 2003, NEW ENGL J MED, V348, P1986, DOI 10.1056/NEJMoa030685; Marra MA, 2003, SCIENCE, V300, P1399, DOI 10.1126/science.1085953; Moitessier N, 2002, J ORG CHEM, V67, P7275, DOI 10.1021/jo0258148; Ohnishi K, 2019, BIOORGAN MED CHEM, V27, P425, DOI 10.1016/j.bmc.2018.12.019; Pillaiyar T, 2016, J MED CHEM, V59, P6595, DOI 10.1021/acs.jmedchem.5b01461; Rota PA, 2003, SCIENCE, V300, P1394, DOI 10.1126/science.1085952; Shimamoto Y, 2015, BIOORGAN MED CHEM, V23, P876, DOI 10.1016/j.bmc.2014.12.028; Thiel V, 2003, J GEN VIROL, V84, P2305, DOI 10.1099/vir.0.19424-0	18	0	0	64	64	PERGAMON-ELSEVIER SCIENCE LTD	OXFORD	THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND	0968-0896	1464-3391		BIOORGAN MED CHEM	Bioorg. Med. Chem.	FEB 15	2020	28	4							115273	10.1016/j.bmc.2019.115273			11	Biochemistry & Molecular Biology; Chemistry, Medicinal; Chemistry, Organic	Biochemistry & Molecular Biology; Pharmacology & Pharmacy; Chemistry	KF8CA	WOS:000509463700004	31926775				2020-04-01	
J	Xu, XW; Wu, XX; Jiang, XG; Xu, KJ; Ying, LJ; Ma, CL; Li, SB; Wang, HY; Zhang, S; Gao, HN; Sheng, JF; Cai, HL; Qiu, YQ; Li, LJ				Xu, Xiao-Wei; Wu, Xiao-Xin; Jiang, Xian-Gao; Xu, Kai-Jin; Ying, Ling-Jun; Ma, Chun-Lian; Li, Shi-Bo; Wang, Hua-Ying; Zhang, Sheng; Gao, Hai-Nv; Sheng, Ji-Fang; Cai, Hong-Liu; Qiu, Yun-Qing; Li, Lan-Juan			Clinical findings in a group of patients infected with the 2019 novel coronavirus (SARS-Cov-2) outside of Wuhan, China: retrospective case series	BMJ-BRITISH MEDICAL JOURNAL			English	Article								OBJECTIVE To study the clinical characteristics of patients in Zhejiang province, China, infected with the 2019 severe acute respiratory syndrome coronavirus 2 (SARS-Cov-2) responsible for coronavirus disease 2019 (covid-2019). DESIGN Retrospective case series. SETTING Seven hospitals in Zhejiang province, China. PARTICIPANTS 62 patients admitted to hospital with laboratory confirmed SARS-Cov-2 infection. Data were collected from 10 January 2020 to 26 January 2020. MAIN OUTCOME MEASURES Clinical data, collected using a standardised case report form, such as temperature, history of exposure, incubation period. If information was not clear, the working group in Hangzhou contacted the doctor responsible for treating the patient for clarification. RESULTS Of the 62 patients studied (median age 41 years), only one was admitted to an intensive care unit, and no patients died during the study. According to research, none of the infected patients in Zhejiang province were ever exposed to the Huanan seafood market, the original source of the virus; all studied cases were infected by human to human transmission. The most common symptoms at onset of illness were fever in 48 (77%) patients, cough in 50 (81%), expectoration in 35 (56%), headache in 21 (34%), myalgia or fatigue in 32 (52%), diarrhoea in 3 (8%), and haemoptysis in 2 (3%). Only two patients (3%) developed shortness of breath on admission. The median time from exposure to onset of illness was 4 days (interquartile range 3-5 days), and from onset of symptoms to first hospital admission was 2 (1-4) days. CONCLUSION As of early February 2020, compared with patients initially infected with SARS-Cov-2 in Wuhan, the symptoms of patients in Zhejiang province are relatively mild.	[Xu, Xiao-Wei; Wu, Xiao-Xin; Xu, Kai-Jin; Sheng, Ji-Fang; Cai, Hong-Liu; Qiu, Yun-Qing; Li, Lan-Juan] Zhejiang Univ, Affiliated Hosp 1, Coll Med,State Key Lab Diag & Treatment Infect Di, Natl Clin Res Ctr Infect Dis,Collaborat Innovat C, Hangzhou 310003, Zhejiang, Peoples R China; [Jiang, Xian-Gao] Wenzhou Cent Hosp, Dept Infect Dis, Wenzhou, Zhejiang, Peoples R China; [Ying, Ling-Jun] Enze Hosp, Taizhou Enze Med Ctr Grp, Dept Infect Dis, Taizhou, Zhejiang, Peoples R China; [Ma, Chun-Lian] First Peoples Hosp Wenling, Dept Infect Dis, Wenling, Zhejiang, Peoples R China; [Li, Shi-Bo] Wenzhou Med Univ, Affiliated Zhoushan Hosp, Dept Infect Dis, Zhoushan, Zhejiang, Peoples R China; [Wang, Hua-Ying] Ningbo Univ, Affiliated Yinzhou Hosp, Yinzhou Peoples Hosp, Coll Med,Dept Resp & Crit Care Med, Ningbo, Zhejiang, Peoples R China; [Zhang, Sheng] Taizhou Hosp, Dept Infect Dis, Taizhou, Zhejiang, Peoples R China; [Gao, Hai-Nv] Zhejiang Shuren Univ, ShuLan Hangzhou Hosp, Shulan Int Med Coll, Dept Infect Dis, Hangzhou, Zhejiang, Peoples R China	Li, LJ (reprint author), Zhejiang Univ, Affiliated Hosp 1, Coll Med,State Key Lab Diag & Treatment Infect Di, Natl Clin Res Ctr Infect Dis,Collaborat Innovat C, Hangzhou 310003, Zhejiang, Peoples R China.	ljli@zju.edu.cn					Chan JFW, 2020, LANCET, V395, P514, DOI 10.1016/S0140-6736(20)30154-9; Chen NS, 2020, LANCET, V395, P507, DOI 10.1016/S0140-6736(20)30211-7; Chu CM, 2004, THORAX, V59, P252, DOI 10.1136/thorax.2003.012658; de Groot RJ, 2013, J VIROL, V87, P7790, DOI 10.1128/JVI.01244-13; Huang CL, 2020, LANCET, V395, P497, DOI 10.1016/S0140-6736(20)30183-5; Ji W, 2020, J MED VIROL, V92, P433, DOI 10.1002/jmv.25682; Kim KH, 2017, J HOSP INFECT, V95, P207, DOI 10.1016/j.jhin.2016.10.008; Lu HZ, 2020, J MED VIROL, V92, P401, DOI 10.1002/jmv.25678; Lu RJ, 2020, LANCET, V395, P565, DOI 10.1016/S0140-6736(20)30251-8; World Health Organization, 2019, MIDDL E RESP SYNDR C; World Health Organization, 2020, NOV COR 2019 NCOV SI; World Health Organization, MIDDL E RESP SYNDR C; World Health Organization, 2020, CLIN MAN SEV AC RESP; Xu XT, 2020, SCI CHINA LIFE SCI, V63, P457, DOI 10.1007/s11427-020-1637-5; Zaki AM, 2012, NEW ENGL J MED, V367, P1814, DOI 10.1056/NEJMoa1211721	15	2	2	62	62	BMJ PUBLISHING GROUP	LONDON	BRITISH MED ASSOC HOUSE, TAVISTOCK SQUARE, LONDON WC1H 9JR, ENGLAND	1756-1833			BMJ-BRIT MED J	BMJ-British Medical Journal	FEB 19	2020	368								m606	10.1136/bmj.m606			7	Medicine, General & Internal	General & Internal Medicine	KO0KS	WOS:000515235300008	32075786	Other Gold			2020-04-01	
J	Baruah, V; Bose, S				Baruah, Vargab; Bose, Sujoy			Immunoinformatics-aided identification of T cell and B cell epitopes in the surface glycoprotein of 2019-nCoV	JOURNAL OF MEDICAL VIROLOGY			English	Article						2019-nCoV; coronavirus; COVID-19; epitope prediction; immunoinformatics; SARS-CoV-2	PREDICTION; PROTEIN; EVOLUTION	The 2019 novel coronavirus (2019-nCoV) outbreak has caused a large number of deaths with thousands of confirmed cases worldwide, especially in East Asia. This study took an immunoinformatics approach to identify significant cytotoxic T lymphocyte (CTL) and B cell epitopes in the 2019-nCoV surface glycoprotein. Also, interactions between identified CTL epitopes and their corresponding major histocompatibility complex (MHC) class I supertype representatives prevalent in China were studied by molecular dynamics simulations. We identified five CTL epitopes, three sequential B cell epitopes and five discontinuous B cell epitopes in the viral surface glycoprotein. Also, during simulations, the CTL epitopes were observed to be binding MHC class I peptide-binding grooves via multiple contacts, with continuous hydrogen bonds and salt bridge anchors, indicating their potential in generating immune responses. Some of these identified epitopes can be potential candidates for the development of 2019-nCoV vaccines.	[Baruah, Vargab; Bose, Sujoy] Gauhati Univ, Dept Biotechnol, Gauhati 781014, Assam, India	Baruah, V (reprint author), Gauhati Univ, Dept Biotechnol, Gauhati 781014, Assam, India.	vargabbarua@gauhati.ac.in		Baruah, Vargab/0000-0002-2882-7785			Bhasin M, 2004, VACCINE, V22, P3195, DOI 10.1016/j.vaccine.2004.02.005; Doytchinova IA, 2007, BMC BIOINFORMATICS, V8, DOI 10.1186/1471-2105-8-4; Du LY, 2009, NAT REV MICROBIOL, V7, P226, DOI 10.1038/nrmicro2090; Fernando BG, 2016, BIOMED RES INT, V2016, P1; Francisco RD, 2015, IMMUNOGENETICS, V67, P651, DOI 10.1007/s00251-015-0875-9; Guest JD, 2018, FRONT IMMUNOL, V9, DOI 10.3389/fimmu.2018.01117; Huang J, 2012, BMC IMMUNOL, V13, DOI 10.1186/1471-2172-13-50; Jespersen MC, 2017, NUCLEIC ACIDS RES, V45, pW24, DOI 10.1093/nar/gkx346; Ji W, 2019, MOL IMMUNOL, V112, P274, DOI 10.1016/j.molimm.2019.06.005; Jurtz V, 2017, J IMMUNOL, V199, P3360, DOI 10.4049/jimmunol.1700893; Kallberg M, 2012, NAT PROTOC, V7, P1511, DOI 10.1038/nprot.2012.085; Larsen MV, 2007, BMC BIOINFORMATICS, V8, DOI 10.1186/1471-2105-8-424; Li F, 2016, ANNU REV VIROL, V3, P237, DOI 10.1146/annurev-virology-110615-042301; Lovell SC, 2003, PROTEINS, V50, P437, DOI 10.1002/prot.10286; Pierce BG, 2014, BIOINFORMATICS, V30, P1771, DOI 10.1093/bioinformatics/btu097; Ponomarenko J, 2008, BMC BIOINFORMATICS, V9, DOI 10.1186/1471-2105-9-514; ROGNAN D, 1992, EUR J BIOCHEM, V208, P101, DOI 10.1111/j.1432-1033.1992.tb17163.x; Saha S, 2006, PROTEINS, V65, P40, DOI 10.1002/prot.21078; Salemi M, 2004, J VIROL, V78, P1602, DOI 10.1128/JVI.78.3.1602-1603.2004; Shen YM, 2014, J CHEM THEORY COMPUT, V10, P4745, DOI 10.1021/ct500592m; Sievers F, 2011, MOL SYST BIOL, V7, DOI 10.1038/msb.2011.75; SMITH TF, 1981, J MOL BIOL, V147, P195, DOI 10.1016/0022-2836(81)90087-5; Ul Qamar MT, 2019, J TRANSL MED, V17, DOI 10.1186/s12967-019-2116-8; Wieczorek M, 2017, FRONT IMMUNOL, V8, DOI 10.3389/fimmu.2017.00292; World Health Organization, 2020 WHO; Zhou YS, 2018, EXPERT REV VACCINES, V17, P677, DOI 10.1080/14760584.2018.1506702	26	0	0	59	59	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0146-6615	1096-9071		J MED VIROL	J. Med. Virol.	MAY	2020	92	5					495	500		10.1002/jmv.25698		MAR 2020	6	Virology	Virology	KU3AY	WOS:000517679900001	32022276	Bronze			2020-04-01	
J	Ena, J; Wenzel, RP				Ena, J.; Wenzel, R. P.			A Novel Coronavirus Emerges	REVISTA CLINICA ESPANOLA			Spanish	Editorial Material									[Ena, J.] Hosp Marina Baixa, Dept Med Interna, Alicante, Spain; [Wenzel, R. P.] Virginia Commonwealth Univ, Dept Med Interna, Richmond, VA USA	Ena, J (reprint author), Hosp Marina Baixa, Dept Med Interna, Alicante, Spain.	ena_jav@yahoo.es					Assiri A, 2013, NEW ENGL J MED, V369, P407, DOI 10.1056/NEJMoa1306742; Azhar EI, 2019, INFECT DIS CLIN N AM, V33, P891, DOI 10.1016/j.idc.2019.08.001; Centers for Disease Control and Prevention Health Alert Network, UPD INT GUID OUTBR 2; de Wilde AH, 2018, CURR TOP MICROBIOL, V419, P1, DOI 10.1007/82_2017_25; Graham BS, 2018, JAMA-J AM MED ASSOC, V319, P1431, DOI 10.1001/jama.2018.0345; Huang CL, 2020, LANCET, V395, P497, DOI 10.1016/S0140-6736(20)30183-5; Ji W, 2020, J MED VIROL, V92, P433, DOI 10.1002/jmv.25682; Ksiazek TG, 2003, NEW ENGL J MED, V348, P1953, DOI 10.1056/NEJMoa030781; WHO, 2020, INF PREV CONTR HLTH; Yuan L., 2020, NY TIMES	10	0	0	59	59	EDICIONES DOYMA S A	BARCELONA	TRAV DE GRACIA 17-21, 08021 BARCELONA, SPAIN	0014-2565	1578-1860		REV CLIN ESP	Rev. Clin. Esp.	MAR	2020	220	2					115	116		10.1016/j.rce.2020.01.001			2	Medicine, General & Internal	General & Internal Medicine	KM6CE	WOS:000514225400006	32063263	Bronze			2020-04-01	
J	Carlos, WG; Dela Cruz, CS; Cao, B; Pasnick, S; Jamil, S				Carlos, W. Graham; Dela Cruz, Charles S.; Cao, Bin; Pasnick, Susan; Jamil, Shazia			Novel Wuhan (2019-nCoV) Coronavirus	AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE			English	Editorial Material												Ribeiro, Nuno/AAH-2299-2020				Huang CL, 2020, LANCET, V395, P497, DOI 10.1016/S0140-6736(20)30183-5; Munster VJ, 2020, NEW ENGL J MED, V382, P692, DOI 10.1056/NEJMp2000929; Zhu N, 2020, NEW ENGL J MED, V382, P727, DOI 10.1056/NEJMoa2001017	3	2	2	59	59	AMER THORACIC SOC	NEW YORK	25 BROADWAY, 18 FL, NEW YORK, NY 10004 USA	1073-449X	1535-4970		AM J RESP CRIT CARE	Am. J. Respir. Crit. Care Med.	FEB 15	2020	201	4					P7	P8					2	Critical Care Medicine; Respiratory System	General & Internal Medicine; Respiratory System	KM5GK	WOS:000514164200001	32004066	Bronze			2020-04-01	
J	Xu, XT; Chen, P; Wang, JF; Feng, JN; Zhou, H; Li, X; Zhong, W; Hao, P				Xu, Xintian; Chen, Ping; Wang, Jingfang; Feng, Jiannan; Zhou, Hui; Li, Xuan; Zhong, Wu; Hao, Pei			Evolution of the novel coronavirus from the ongoing Wuhan outbreak and modeling of its spike protein for risk of human transmission	SCIENCE CHINA-LIFE SCIENCES			English	Letter							RECEPTOR		[Xu, Xintian; Hao, Pei] Chinese Acad Sci, Inst Pasteur Shanghai, Ctr Biosafety Mega Sci, Key Lab Mol Virol & Immunol, Shanghai 200031, Peoples R China; [Chen, Ping; Zhou, Hui; Li, Xuan] Chinese Acad Sci, CAS Ctr Excellence Mol Plant Sci, Key Lab Synthet Biol, Shanghai 200032, Peoples R China; [Wang, Jingfang] Shanghai Jiao Tong Univ, Shanghai Ctr Syst Biomed, Key Lab Syst Biomed, Minist Educ, Shanghai 200240, Peoples R China; [Feng, Jiannan; Zhong, Wu] Beijing Inst Pharmacol & Toxicol, Natl Engn Res Ctr Emergence Drugs, Beijing 100850, Peoples R China; [Chen, Ping; Hao, Pei] Guangzhou Med Univ, Guangzhou Women & Childrens Med Ctr, Joint Program Infect & Immun, Guangzhou 510623, Guangdong, Peoples R China; [Chen, Ping; Hao, Pei] Chinese Acad Sci, Inst Pasteur Shanghai, Joint Program Infect & Immun, Shanghai 200031, Peoples R China	Hao, P (reprint author), Chinese Acad Sci, Inst Pasteur Shanghai, Ctr Biosafety Mega Sci, Key Lab Mol Virol & Immunol, Shanghai 200031, Peoples R China.; Li, X (reprint author), Chinese Acad Sci, CAS Ctr Excellence Mol Plant Sci, Key Lab Synthet Biol, Shanghai 200032, Peoples R China.; Zhong, W (reprint author), Beijing Inst Pharmacol & Toxicol, Natl Engn Res Ctr Emergence Drugs, Beijing 100850, Peoples R China.; Hao, P (reprint author), Guangzhou Med Univ, Guangzhou Women & Childrens Med Ctr, Joint Program Infect & Immun, Guangzhou 510623, Guangdong, Peoples R China.; Hao, P (reprint author), Chinese Acad Sci, Inst Pasteur Shanghai, Joint Program Infect & Immun, Shanghai 200031, Peoples R China.	lixuan@sippe.ac.cn; zhongwu@bmi.ac.cn; phao@ips.ac.cn			National Science and Technology Major Projects for "Major New Drugs Innovation and Development" of China [2018ZX09711003]; National Key R&D Program of China [2018YFC0310600]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [31771412]; Special Fund for strategic bio-resources from Chinese Academy of Sciences [ZSYS-014]	This work was supported in part by grants from the National Science and Technology Major Projects for "Major New Drugs Innovation and Development" (directed by Dr. Song Li) (2018ZX09711003) of China, the National Key R&D Program (2018YFC0310600) of China, the National Natural Science Foundation of China (31771412), and Special Fund for strategic bio-resources from Chinese Academy of Sciences (ZSYS-014). We also acknowledge the National Institute for Viral Disease Control and Prevention, China CDC; Wuhan Institute of Virology, Chinese Academy of Sciences; Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College; and Wuhan Jinyintan Hospital for their efforts in research and collecting the data and genome sequencing sharing. In addition, we acknowledge GISAID (https://www.gisaid.org/) for facilitating open data sharing.	CDC, 2020, 2019 NOV COR 2019 NC; Cotten M, 2013, LANCET, V382, P1993, DOI 10.1016/S0140-6736(13)61887-5; Hu B, 2017, PLOS PATHOG, V13, DOI 10.1371/journal.ppat.1006698; Li F, 2005, SCIENCE, V309, P1864, DOI 10.1126/science.1116480; Li F, 2012, J VIROL, V86, P2856, DOI 10.1128/JVI.06882-11; Maier JA, 2015, J CHEM THEORY COMPUT, V11, P3696, DOI 10.1021/acs.jctc.5b00255; Normile D, 2020, MYSTERY VIRUS FOUND; Raj VS, 2013, NATURE, V495, P251, DOI 10.1038/nature12005; Schwede T, 2003, NUCLEIC ACIDS RES, V31, P3381, DOI 10.1093/nar/gkg520; Shu Y, 2017, EUROSURVEILLANCE; World Health Organization, 2020, PNEUM UNKN CAUS CHIN; Zhang YZ, 2020, INITIAL GENOME RELEA	12	14	14	59	59	SCIENCE PRESS	BEIJING	16 DONGHUANGCHENGGEN NORTH ST, BEIJING 100717, PEOPLES R CHINA	1674-7305	1869-1889		SCI CHINA LIFE SCI	Sci. China-Life Sci.	MAR	2020	63	3					457	460		10.1007/s11427-020-1637-5		JAN 2020	4	Biology	Life Sciences & Biomedicine - Other Topics	KU1DB	WOS:000510426500001	32009228	Bronze			2020-04-01	
J	Li, GD; De Clercq, E				Li, Guangdi; De Clercq, Erik			Therapeutic options for the 2019 novel coronavirus (2019-nCoV)	NATURE REVIEWS DRUG DISCOVERY			English	Editorial Material								Therapeutic options in response to the 2019-nCoV outbreak are urgently needed. Here, we discuss the potential for repurposing existing antiviral agents to treat 2019-nCoV infection (now known as COVID-19), some of which are already moving into clinical trials.	[Li, Guangdi] Cent South Univ, Xiangya Sch Publ Hlth, Dept Epidemiol & Hlth Stat, Changsha, Peoples R China; [De Clercq, Erik] Katholieke Univ Leuven, Rega Inst Med Res, Dept Microbiol Immunol & Transplantat, Leuven, Belgium	Li, GD (reprint author), Cent South Univ, Xiangya Sch Publ Hlth, Dept Epidemiol & Hlth Stat, Changsha, Peoples R China.; De Clercq, E (reprint author), Katholieke Univ Leuven, Rega Inst Med Res, Dept Microbiol Immunol & Transplantat, Leuven, Belgium.	liguangdi.research@gmail.com; erik.declercq@kuleuven.be			National Nature Science Foundation of ChinaNational Natural Science Foundation of China [31571368, 31871324, 81730064]; National Science and Technology Major Project [2018ZX10715004]; Natural Science Foundation of Hunan ProvinceNatural Science Foundation of Hunan Province [2018JJ3713]; Hunan Youth Elite Project [2018RS3006]; Project of Innovation-Driven Plan of Central South University [2016CX031]	We would like to thank P. Kirkpatrick for his critical comments and editorial assistance to improve this article. This work was supported by the National Nature Science Foundation of China (grant numbers 31571368, 31871324 and 81730064), the National Science and Technology Major Project (grant number 2018ZX10715004), the Natural Science Foundation of Hunan Province (grant number 2018JJ3713), the Hunan Youth Elite Project (grant number 2018RS3006) and the Project of Innovation-Driven Plan of Central South University (grant 2016CX031).	Clercq E, 2016, CLIN MICROBIOL REV, V29, P695, DOI DOI 10.1128/CMR.00102-15.ADDRESS; De Clercq E, 2019, CHEM-ASIAN J, V14, P3962, DOI 10.1002/asia.201900841; Holshue ML, 2020, NEW ENGL J MED, V382, P929, DOI 10.1056/NEJMoa2001191; Morse JS, 2020, CHEMBIOCHEM, V21, P730, DOI 10.1002/cbic.202000047; Wang ML, 2020, CELL RES, V30, P269, DOI 10.1038/s41422-020-0282-0; Zumla A, 2016, NAT REV DRUG DISCOV, V15, P327, DOI 10.1038/nrd.2015.37	6	3	3	57	57	NATURE PUBLISHING GROUP	LONDON	MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND	1474-1776	1474-1784		NAT REV DRUG DISCOV	Nat. Rev. Drug Discov.	MAR	2020	19	3					149	150		10.1038/d41573-020-00016-0			2	Biotechnology & Applied Microbiology; Pharmacology & Pharmacy	Biotechnology & Applied Microbiology; Pharmacology & Pharmacy	KS2PH	WOS:000518151800001	32127666	Bronze			2020-04-01	
J	Liu, J; Zheng, X; Tong, QX; Li, W; Wang, BJ; Sutter, K; Trilling, M; Lu, MJ; Dittmer, U; Yang, DL				Liu, Jia; Zheng, Xin; Tong, Qiaoxia; Li, Wei; Wang, Baoju; Sutter, Kathrin; Trilling, Mirko; Lu, Mengji; Dittmer, Ulf; Yang, Dongliang			Overlapping and discrete aspects of the pathology and pathogenesis of the emerging human pathogenic coronaviruses SARS-CoV, MERS-CoV, and 2019-nCoV	JOURNAL OF MEDICAL VIROLOGY			English	Review						coronavirus; immnopathology; immune responses; pathogenesis; respiratory tract; virus classification	RESPIRATORY SYNDROME CORONAVIRUS; IN-SITU HYBRIDIZATION; INFLAMMATORY CYTOKINES; INTERFERON; PNEUMONIA; INFECTION; REPLICATION; CHEMOKINES; RESPONSES; OUTBREAK	First reported from Wuhan, The People's Republic of China, on 31 December 2019, the ongoing outbreak of a novel coronavirus (2019-nCoV) causes great global concerns. Based on the advice of the International Health Regulations Emergency Committee and the fact that to date 24 other countries also reported cases, the WHO Director-General declared that the outbreak of 2019-nCoV constitutes a Public Health Emergency of International Concern on 30 January 2020. Together with the other two highly pathogenic coronaviruses, the severe acute respiratory syndrome coronavirus (SARS-CoV) and Middle East respiratory syndrome coronavirus (MERS-CoV), 2019-nCov and other yet to be identified coronaviruses pose a global threat to public health. In this mini-review, we provide a brief introduction to the pathology and pathogenesis of SARS-CoV and MERS-CoV and extrapolate this knowledge to the newly identified 2019-nCoV.	[Liu, Jia; Zheng, Xin; Tong, Qiaoxia; Li, Wei; Wang, Baoju; Yang, Dongliang] Huazhong Univ Sci & Technol, Tongji Med Coll, Union Hosp, Dept Infect Dis, Wuhan 430022, Hubei, Peoples R China; [Sutter, Kathrin; Trilling, Mirko; Lu, Mengji; Dittmer, Ulf] Univ Duisburg Essen, Univ Hosp Essen, Inst Virol, Essen, Germany; [Liu, Jia; Zheng, Xin; Wang, Baoju; Sutter, Kathrin; Trilling, Mirko; Lu, Mengji; Dittmer, Ulf; Yang, Dongliang] Huazhong Univ Sci & Technol, Joint Int Lab Infect & Immun, Wuhan, Hubei, Peoples R China	Liu, J; Yang, DL (reprint author), Huazhong Univ Sci & Technol, Tongji Med Coll, Union Hosp, Dept Infect Dis, Wuhan 430022, Hubei, Peoples R China.	jialiu77@hust.edu.cn; dlyang@hust.edu.cn	Ribeiro, Nuno/AAH-2299-2020	Liu, Jia/0000-0002-8262-4997	National Scientific and Technological Major Project of China [2017ZX10202203]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81861138044, 91742114, 91642118]	National Scientific and Technological Major Project of China, Grant/Award Number: 2017ZX10202203; National Natural Science Foundation of China, Grant/Award Numbers: 81861138044, 91742114, 91642118	Alsaad KO, 2018, HISTOPATHOLOGY, V72, P516, DOI 10.1111/his.13379; Bradley BT, 2019, SEMIN DIAGN PATHOL, V36, P152, DOI 10.1053/j.semdp.2019.04.006; Cameron MJ, 2007, J VIROL, V81, P8692, DOI 10.1128/JVI.00527-07; Cha RH, 2016, J KOREAN MED SCI, V31, P635, DOI 10.3346/jkms.2016.31.4.635; Chan JFW, 2020, LANCET, V395, P514, DOI 10.1016/S0140-6736(20)30154-9; Channappanavar R, 2016, CELL HOST MICROBE, V19, P181, DOI 10.1016/j.chom.2016.01.007; Chen NS, 2020, LANCET, V395, P507, DOI 10.1016/S0140-6736(20)30211-7; Chen Y, 2020, J MED VIROL, V92, P418, DOI 10.1002/jmv.25681; Chien JY, 2006, RESPIROLOGY, V11, P715, DOI 10.1111/j.1440-1843.2006.00942.x; Chu H, 2016, J INFECT DIS, V213, P904, DOI 10.1093/infdis/jiv380; Chu H, 2014, VIROLOGY, V454, P197, DOI 10.1016/j.virol.2014.02.018; Ding YQ, 2003, J PATHOL, V200, P282, DOI 10.1002/path.1440; Gu J, 2005, J EXP MED, V202, P415, DOI 10.1084/jem.20050828; Huang CL, 2020, LANCET, V395, P497, DOI 10.1016/S0140-6736(20)30183-5; Huang KJ, 2005, J MED VIROL, V75, P185, DOI 10.1002/jmv.20255; Kong SL, 2009, VIRUS RES, V145, P260, DOI 10.1016/j.virusres.2009.07.014; Kupferschmidt K, 2013, SCIENCE, V341, P702, DOI 10.1126/science.341.6147.702; Li Qun, 2020, N Engl J Med, V382, P1199, DOI 10.1056/NEJMoa2001316; Li WD, 2005, SCIENCE, V310, P676, DOI 10.1126/science.1118391; Menachery VD, 2014, MBIO, V5, DOI 10.1128/mBio.01174-14; Meyerholz DK, 2016, AM J PATHOL, V186, P78, DOI 10.1016/j.ajpath.2015.09.014; Ng DL, 2016, AM J PATHOL, V186, P652, DOI 10.1016/j.ajpath.2015.10.024; Nicholls JM, 2003, LANCET, V361, P1773, DOI 10.1016/S0140-6736(03)13413-7; Peiris JSM, 2003, LANCET, V361, P1767, DOI 10.1016/S0140-6736(03)13412-5; Ren LL, 2020, CHIN MED J, P1; Shieh WJ, 2005, HUM PATHOL, V36, P303, DOI 10.1016/j.humpath.2004.11.006; Song ZQ, 2019, VIRUSES-BASEL, V11, DOI 10.3390/v11010059; To K, 2004, J PATHOL, V202, P157, DOI 10.1002/path.1510; Walker DH, 2016, AM J PATHOL, V186, P507, DOI 10.1016/j.ajpath.2015.11.001; Wang C, 2020, LANCET, V395, P470, DOI 10.1016/S0140-6736(20)30185-9; Widagdo W, 2016, J VIROL, V90, P4838, DOI 10.1128/JVI.02994-15; Wong CK, 2004, CLIN EXP IMMUNOL, V136, P95, DOI 10.1111/j.1365-2249.2004.02415.x; Yeung ML, 2016, NAT MICROBIOL, V1, DOI [10.1038/NMICROBIOL.2016.4, 10.1038/nmicrobiol.2016.4]; Zhang YC, 2004, INFECT IMMUN, V72, P4410, DOI 10.1128/IAI.72.8.4410-4415.2004; Zhou J, 2014, J INFECT DIS, V209, P1331, DOI 10.1093/infdis/jit504; Zhou P, 2020, NATURE, DOI 10.1038/s41586-020-2012-7; Zhu N, 2020, NEW ENGL J MED, V382, P727, DOI 10.1056/NEJMoa2001017	37	0	0	57	57	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0146-6615	1096-9071		J MED VIROL	J. Med. Virol.	MAY	2020	92	5					491	494		10.1002/jmv.25709		FEB 2020	4	Virology	Virology	KU3AY	WOS:000514677000001	32056249	Bronze			2020-04-01	
J							Eurosurveillance Editorial Team	Note from the editors: novel coronavirus (2019-nCoV)	EUROSURVEILLANCE			English	Editorial Material								At the end of 2019, on 31 December, the World Health Organization (WHO) Country Office in China was informed of cases of pneumonia of unknown aetiology that had been detected in Wuhan, a city in the province of Hubei. Just a day earlier, on 30 December, ProMED-mail published a post calling for more information about 'A cluster of undiagnosed pneumonia China (Hubei)'. The post indicated that there had been some 20 patients diagnosed with 'atypical pneumonia' with seven of them being severely ill [1]. In their accompanying note, the post's moderator stated 'the type of social media activity that is now surrounding this event, is very reminiscent of the original "rumors" that accompanied the SARS-CoV outbreak.' [Ed. severe acute respiratory syndrome (SARS) coronavirus outbreak in 2003].	[Eurosurveillance Editorial Team] European Ctr Dis Prevent & Control ECDC, Stockholm, Sweden	European Ctr Dis Prevent & Control ECDC, Stockholm, Sweden.		Ribeiro, Nuno/AAH-2299-2020				Corman VM, 2020, EUROSURVEILLANCE, V25, P23, DOI 10.2807/1560-7917.ES.2020.25.3.2000045; ECDC, 2020, RISK ASS OUTBR AC RE; European Centre for Disease Prevention and Control (ECDC), 2019, NOV COR CHIN; World Health Organization, 2020, LAB TEST 2019 NOV CO; Wu P, 2020, EUROSURVEILLANCE, V25, P4, DOI 10.2807/1560-7917.ES.2020.25.3.2000044; Zhang Y-Z, VIROLOGICAL	6	0	0	57	57	EUR CENTRE DIS PREVENTION & CONTROL	STOCKHOLM	TOMTEBODAVAGEN 11A, STOCKHOLM, 171 83, SWEDEN	1560-7917			EUROSURVEILLANCE	Eurosurveillance	JAN 23	2020	25	3					2	3		10.2807/1560-7917.ES.2020.25.3.2001231			2	Infectious Diseases	Infectious Diseases	KF6OZ	WOS:000509360400001	31992390	DOAJ Gold, Green Published			2020-04-01	
J	Du Toit, A				Du Toit, Andrea			Outbreak of a novel coronavirus	NATURE REVIEWS MICROBIOLOGY			English	Editorial Material								The emergence of a new coronavirus in China raises global alarm.			nrmicro@nature.com	Ribeiro, Nuno/AAH-2299-2020					0	1	1	55	55	NATURE PUBLISHING GROUP	LONDON	MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND	1740-1526	1740-1534		NAT REV MICROBIOL	Nat. Rev. Microbiol.	MAR	2020	18	3					123	123		10.1038/s41579-020-0332-0			1	Microbiology	Microbiology	KM4YW	WOS:000514142000002	31988490	Bronze			2020-04-01	
J	Rubin, EJ; Baden, LR; Morrissey, S; Campion, EW				Rubin, Eric J.; Baden, Lindsey R.; Morrissey, Stephen; Campion, Edward W.			Medical Journals and the 2019-nCoV Outbreak	NEW ENGLAND JOURNAL OF MEDICINE			English	Editorial Material								The editors announce expedited handling for submitted manuscripts describing the 2019-nCoV outbreak and make broad recommendations for speed, transparency, and data sharing.									0	0	0	55	55	MASSACHUSETTS MEDICAL SOC	WALTHAM	WALTHAM WOODS CENTER, 860 WINTER ST,, WALTHAM, MA 02451-1413 USA	0028-4793	1533-4406		NEW ENGL J MED	N. Engl. J. Med.	FEB 27	2020	382	9					866	866		10.1056/NEJMe2001329			1	Medicine, General & Internal	General & Internal Medicine	KS8TT	WOS:000518581500013	31986242	Bronze			2020-04-01	
J	Huang, C; Wang, Y; Li, X				Huang, C.; Wang, Y.; Li, X.			Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China (vol 395, pg 497, 2020)	LANCET			English	Correction																Huang CL, 2020, LANCET, V395, P497, DOI 10.1016/S0140-6736(20)30183-5	1	0	0	55	55	ELSEVIER SCIENCE INC	NEW YORK	STE 800, 230 PARK AVE, NEW YORK, NY 10169 USA	0140-6736	1474-547X		LANCET	Lancet	FEB 15	2020	395	10223					496	496		10.1016/S0140-6736(20)30252-X			1	Medicine, General & Internal	General & Internal Medicine	KN1CG	WOS:000514576900031		Bronze			2020-04-01	
J	Lu, HZ				Lu, Hongzhou			Drug treatment options for the 2019-new coronavirus (2019-nCoV)	BIOSCIENCE TRENDS			English	Article						2019-nCoV; Coronaviruses; pneumonia	RESPIRATORY SYNDROME CORONAVIRUS	As of January 22, 2020, a total of 571 cases of the 2019-new coronavirus (2019-nCoV) have been reported in 25 provinces (districts and cities) in China. At present, there is no vaccine or antiviral treatment for human and animal coronavirus, so that identifying the drug treatment options as soon as possible is critical for the response to the 2019-nCoV outbreak. Three general methods, which include existing broad-spectrum antiviral drugs using standard assays, screening of a chemical library containing many existing compounds or databases, and the redevelopment of new specific drugs based on the genome and biophysical understanding of individual coronaviruses, are used to discover the potential antiviral treatment of human pathogen coronavirus. Lopinavir /Ritonavir, Nucleoside analogues, Neuraminidase inhibitors, Remdesivir, peptide (EK1), arbidol, RNA synthesis inhibitors (such as TDF, 3TC), anti-inflammatory drugs (such as hormones and other molecules), Chinese traditional medicine, such ShuFengJieDu Capsules and Lianhuaqingwen Capsule, could be the drug treatment options for 2019-nCoV. However, the efficacy and safety of these drugs for 2019-nCoV still need to be further confirmed by clinical experiments.	[Lu, Hongzhou] Fudan Univ, Sci Res Ctr, Shanghai Publ Hlth Clin Ctr, Shanghai, Peoples R China; [Lu, Hongzhou] Fudan Univ, Shanghai Publ Hlth Clin Ctr, Dept Infect Dis, Shanghai, Peoples R China; [Lu, Hongzhou] Fudan Univ, Dept Infect Dis, Huashan Hosp, Shanghai, Peoples R China	Lu, HZ (reprint author), Fudan Univ, Shanghai Publ Hlth Clin Ctr, 2901 Caolang Rd, Shanghai 201508, Peoples R China.	luhongzhou@fudan.edu.cn			13th Five-Year National Science and Technology Major Project from Ministry of Science and Technology of the People's Republic of China [2017ZX09304027]	This research was funded by the 13th Five-Year National Science and Technology Major Project from Ministry of Science and Technology of the People's Republic of China (Grant No.: 2017ZX09304027).	Agostini ML, 2018, MBIO, V9, DOI 10.1128/mBio.00221-18; Arabi YM, 2018, TRIALS, V19, DOI 10.1186/s13063-017-2427-0; Bleibtreu A, 2018, BMC INFECT DIS, V18, DOI 10.1186/s12879-018-3223-5; Channappanavar R, 2017, J IMMUNOL, V198, P4046, DOI 10.4049/jimmunol.1601896; Chow EJ, 2019, CRIT CARE, V23, DOI 10.1186/s13054-019-2491-9; Chu CM, 2004, THORAX, V59, P252, DOI 10.1136/thorax.2003.012658; Coleman CM, 2016, J VIROL, V90, P8924, DOI 10.1128/JVI.01429-16; Ding YW, 2017, BMC COMPLEM ALTERN M, V17, DOI 10.1186/s12906-017-1585-7; Imai Natsuko, ESTIMATING POTENTIAL; Ji S, 2020, BIOMED PHARMACOTHER, V121, DOI 10.1016/j.biopha.2019.109652; Kim Y, 2016, PLOS PATHOG, V12, DOI 10.1371/journal.ppat.1005531; Lu HZ, 2020, J MED VIROL, V92, P401, DOI 10.1002/jmv.25678; National Health Commission of the People's Republic of China, NOT PRINT DISTR DIAG; National Health Commission of the People's Republic of China, PNEUM EP IT NEW COR; Pang Y, 2019, J VIS COMMUN IMAGE R, V65, DOI 10.1016/j.jvcir.2019.102676; Sheahan Timothy P, 2020, Nat Commun, V11, P222, DOI 10.1038/s41467-019-13940-6; Xia S, 2019, SCI ADV, V5, DOI 10.1126/sciadv.aav4580; Zumla A, 2016, NAT REV DRUG DISCOV, V15, P327, DOI 10.1038/nrd.2015.37	18	6	6	55	55	IRCA-BSSA	TOKYO	PEARL CITY KOISHIKAWA 603, 2-4-5 KASUGA, BUNKYO-KU, TOKYO, 112-0003, JAPAN	1881-7815	1881-7823		BIOSCI TRENDS	BioSci. Trends	FEB	2020	14	1					69	71		10.5582/bst.2020.01020			3	Biology	Life Sciences & Biomedicine - Other Topics	KU2OR	WOS:000519548300011	31996494	Bronze			2020-04-01	
J	[Anonymous]				[Anonymous]			Prevent and predict	NATURE ECOLOGY & EVOLUTION			English	Editorial Material								As the COVID-19 outbreak continues, the next pandemic could be prevented by ending the wildlife trade and reinvesting in the monitoring of potential zoonoses.								Blair ME, 2017, BIOSCIENCE, V67, P994, DOI 10.1093/biosci/bix113; Hu B, 2017, PLOS PATHOG, V13, DOI 10.1371/journal.ppat.1006698; MacGregor H, 2017, PHILOS T R SOC B, V372, DOI 10.1098/rstb.2016.0170; Wallen KE, 2018, NAT CONSERV-BULGARIA, P55, DOI 10.3897/natureconservation.26.22725; Zhou P, 2020, NATURE, DOI 10.1038/s41586-020-2012-7	5	0	0	53	53	NATURE PUBLISHING GROUP	LONDON	MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND	2397-334X			NAT ECOL EVOL	Nat. Ecol. Evol.	MAR	2020	4	3					283	283		10.1038/s41559-020-1150-5		FEB 2020	1	Ecology; Evolutionary Biology	Environmental Sciences & Ecology; Evolutionary Biology	KS0XI	WOS:000514763000002	32080369	Bronze			2020-04-01	
J	Shi, ZH; Wang, WJ; Xu, ZX; Zhao, MM; Lan, YL				Shi, Zhihai; Wang, Wenjia; Xu, Zhaoxue; Zhao, Mengmeng; Lan, Yali			Detection and Molecular Characterization of BCoVs Circulating in Central China Based on the Full-length Spike Gene	KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI			English	Article						BCoV; Spike gene; Phylogenetic analysis; China	BOVINE CORONAVIRUS; DAIRY-CATTLE; DIARRHEA	Bovine coronavirus (BCoV) is prevalent throughout the world and is an important aetiological agent of diarrhoea in new-born calves. However, little is known about the genetic diversity and molecular epidemiology of BCoV in China. In this study, a total of 127 faecal samples from diarrhoeic dairy calves from nine cities in Henan Province, Central China, were collected between 2017 and 2018 and evaluated by RTPCR for the N gene. BCoV was detected in 15% (19/127) of calves. In addition, the full-length sequence of the S gene from 13 representative BCoV strains was obtained and analysed. Sequencing results for the full-length S gene showed 97.3-97.5% nucleotide (nt) identity and 96.3-96.8% amino acid (aa) identity with the classical Mebus strain. Phylogenetic analysis based on the full-length S gene showed that the BCoV strains in this study clustered with Vietnamese and Cuban BCoV strains on a large branch of the tree. These results enrich the molecular characterization of the Chinese BCoV strains and provide the first large-scale epidemiological examination of the prevalence of BCoV in diarrhoeic calves in Henan Province, Central China.	[Shi, Zhihai; Xu, Zhaoxue; Lan, Yali] Henan Acad Agr Sci, Inst Anim Husb & Vet, Zhengzhou 450002, Henan, Peoples R China; [Wang, Wenjia] Henan Univ Anim Husb & Econ, Coll Pharmaceut Engn, Zhengzhou 450046, Henan, Peoples R China; [Shi, Zhihai; Xu, Zhaoxue; Lan, Yali] Henan Key Lab Farm Anim Breeding & Nutr Regulat, Zhengzhou 450002, Henan, Peoples R China; [Zhao, Mengmeng] Foshan Univ, Sch Life Sci & Engn, Foshan 528225, Guangdong, Peoples R China	Lan, YL (reprint author), Henan Acad Agr Sci, Inst Anim Husb & Vet, Zhengzhou 450002, Henan, Peoples R China.; Lan, YL (reprint author), Henan Key Lab Farm Anim Breeding & Nutr Regulat, Zhengzhou 450002, Henan, Peoples R China.; Zhao, MM (reprint author), Foshan Univ, Sch Life Sci & Engn, Foshan 528225, Guangdong, Peoples R China.	mzhao8@tulane.edu; yali2005haonan@sina.com			Key Program for Science and Technology Development of Henan [192102110074]; National Key Research and Development Program of China [2018YFD0501700]; National beef cattle industrial technology system [CARS-37]; Open Program for Buffalo research of Guangxi [SNKF-2015-03]; National Natural Science Foundation of China (NSFC)National Natural Science Foundation of China [31902279]	This work was funded by the Key Program for Science and Technology Development of Henan (192102110074), the National Key Research and Development Program of China 2018YFD0501700), the National beef cattle industrial technology system (CARS-37), the Open Program for Buffalo research of Guangxi (SNKF-2015-03), as well as the National Natural Science Foundation of China (NSFC) (grants 31902279).	Aleanizy FS, 2017, BMC INFECT DIS, V17, DOI 10.1186/s12879-016-2137-3; Castells M, 2019, ARCH VIROL, V164, P2715, DOI 10.1007/s00705-019-04384-w; Dennis AF, 2014, J VIROL, V88, P3789, DOI 10.1128/JVI.03516-13; Fernandes AD, 2018, VET MED SCI, V4, P183, DOI 10.1002/vms3.102; Forni D, 2015, SCI REP-UK, V5, DOI 10.1038/srep14480; Gallagher TM, 2001, VIROLOGY, V279, P371, DOI 10.1006/viro.2000.0757; Gao FL, 2019, ECOL EVOL, V9, P3891, DOI 10.1002/ece3.5015; Giannitti F, 2015, VET PATHOL, V52, P1148, DOI 10.1177/0300985814568683; Gomez DE, 2017, J VET INTERN MED, V31, P1884, DOI 10.1111/jvim.14811; Graham RL, 2010, J VIROL, V84, P3134, DOI 10.1128/JVI.01394-09; Gunn L, 2015, IRISH VET J, V68, DOI 10.1186/s13620-015-0060-3; Keha A, 2019, TRANSBOUND EMERG DIS, V66, P1971, DOI 10.1111/tbed.13228; Li F, 2016, ANNU REV VIROL, V3, P237, DOI 10.1146/annurev-virology-110615-042301; Lojkic I, 2015, BMC VET RES, V11, DOI 10.1186/s12917-015-0511-9; LU CP, 1991, J VET MED B, V38, P473, DOI 10.1111/j.1439-0450.1991.tb00898.x; Masters PS, 2006, ADV VIRUS RES, V66, P193, DOI 10.1016/S0065-3527(06)66005-3; Park SJ, 2006, J CLIN MICROBIOL, V44, P3178, DOI 10.1128/JCM.02667-05; Rambaut A., 2009, FIGTREE V1 4 3; Ribeiro J, 2016, TROP ANIM HEALTH PRO, V48, P649, DOI 10.1007/s11250-015-0975-4; Singasa K, 2017, TROP ANIM HEALTH PRO, V49, P1523, DOI 10.1007/s11250-017-1358-9; YOO DW, 1991, VIROLOGY, V183, P91, DOI 10.1016/0042-6822(91)90121-Q; Yoo DW, 2001, CLIN DIAGN LAB IMMUN, V8, P297, DOI 10.1128/CDLI.8.2.297-302.2001	22	0	0	52	52	KAFKAS UNIV, VETERINER FAKULTESI DERGISI	KARS	KAFKAS UNIV, VETERINER FAKULTESI DERGISI, KARS, 36040, TURKEY	1300-6045			KAFKAS UNIV VET FAK	Kafkas Univ. Vet. Fak. Derg.	MAR-APR	2020	26	2					181	186		10.9775/kvfd.2019.22678			6	Veterinary Sciences	Veterinary Sciences	KF0UR	WOS:000508967700004		DOAJ Gold			2020-04-01	
J	[Anonymous]				[Anonymous]			Emerging understandings of 2019-nCoV	LANCET			English	Editorial Material												Ribeiro, Nuno/AAH-2299-2020					0	8	8	52	52	ELSEVIER SCIENCE INC	NEW YORK	STE 800, 230 PARK AVE, NEW YORK, NY 10169 USA	0140-6736	1474-547X		LANCET	Lancet	FEB 1	2020	395	10221					311	311		10.1016/S0140-6736(20)30186-0			1	Medicine, General & Internal	General & Internal Medicine	KH7WI	WOS:000510860200004	31986259	Bronze			2020-04-01	
J	Li, G; Fan, YH; Lai, YN; Han, TT; Li, ZH; Zhou, PW; Pan, P; Wang, WB; Hu, DW; Liu, XH; Zhang, QW; Wu, JG				Li, Geng; Fan, Yaohua; Lai, Yanni; Han, Tiantian; Li, Zonghui; Zhou, Peiwen; Pan, Pan; Wang, Wenbiao; Hu, Dingwen; Liu, Xiaohong; Zhang, Qiwei; Wu, Jianguo			Coronavirus infections and immune responses	JOURNAL OF MEDICAL VIROLOGY			English	Review						chemokine; coronavirus; cytokines; inflammation; interferon	RESPIRATORY-SYNDROME-CORONAVIRUS; PATTERN-RECOGNITION RECEPTORS; GMP-AMP SYNTHASE; RIG-I; MERS-COV; T-CELLS; RNA-RECOGNITION; SPIKE PROTEIN; CYTOSOLIC DNA; MOUSE MODEL	Coronaviruses (CoVs) are by far the largest group of known positive-sense RNA viruses having an extensive range of natural hosts. In the past few decades, newly evolved Coronaviruses have posed a global threat to public health. The immune response is essential to control and eliminate CoV infections, however, maladjusted immune responses may result in immunopathology and impaired pulmonary gas exchange. Gaining a deeper understanding of the interaction between Coronaviruses and the innate immune systems of the hosts may shed light on the development and persistence of inflammation in the lungs and hopefully can reduce the risk of lung inflammation caused by CoVs. In this review, we provide an update on CoV infections and relevant diseases, particularly the host defense against CoV-induced inflammation of lung tissue, as well as the role of the innate immune system in the pathogenesis and clinical treatment.	[Li, Geng; Zhou, Peiwen; Wang, Wenbiao; Zhang, Qiwei; Wu, Jianguo] Jinan Univ, Inst Med Microbiol, Guangdong Prov Key Lab Virol, Guangzhou 510632, Peoples R China; [Li, Geng; Li, Zonghui; Pan, Pan] Guangzhou Univ Chinese Med, Lab Anim Ctr, Guangzhou, Peoples R China; [Fan, Yaohua; Lai, Yanni; Han, Tiantian] Guangzhou Univ Chinese Med, Clin Med Coll 1, Guangzhou, Peoples R China; [Hu, Dingwen; Wu, Jianguo] Wuhan Univ, Coll Life Sci, State Key Lab Virol, Wuhan, Peoples R China; [Liu, Xiaohong] Guangzhou Univ Chinese Med, Affiliated Hosp 1, Guangzhou, Peoples R China; [Zhang, Qiwei] Southern Med Univ, Sch Pub Hlth, Guangzhou, Peoples R China	Wu, JG (reprint author), Jinan Univ, Inst Med Microbiol, Guangdong Prov Key Lab Virol, Guangzhou 510632, Peoples R China.	jwu898@jnu.edu.cn			National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81730061, 81902066, 81471942]	National Natural Science Foundation of China, Grant/Award Numbers: 81730061, 81902066, 81471942	Ababneh M, 2019, VET WORLD, V12, P1554, DOI 10.14202/vetworld.2019.1554-1562; Agostini L, 2004, IMMUNITY, V20, P319, DOI 10.1016/S1074-7613(04)00046-9; Akira S, 2006, CELL, V124, P783, DOI 10.1016/j.cell.2006.02.015; Ali MT, 2014, BIOINFORMATION, V10, P533, DOI 10.6026/97320630010533; Baccala R, 2009, NAT REV RHEUMATOL, V5, P448, DOI 10.1038/nrrheum.2009.136; Baker S, 2019, PLOS ONE, V14, DOI 10.1371/journal.pone.0217626; Bunte K, 2019, INT J MOL SCI, V20, DOI 10.3390/ijms20143394; Campbell O, 2019, LETT APPL MICROBIOL, V69, P353, DOI 10.1111/lam.13213; Cao L, 2019, PLOS PATHOG, V15, DOI 10.1371/journal.ppat.1008079; Cardone M, 2015, J VIROL, V89, P4798, DOI 10.1128/JVI.03681-14; Cecere TE, 2012, VIRUSES-BASEL, V4, P833, DOI 10.3390/v4050833; Chan JFW, 2015, CLIN MICROBIOL REV, V28, P465, DOI 10.1128/CMR.00102-14; Channappanavar R, 2014, J VIROL, V88, P11034, DOI 10.1128/JVI.01505-14; Chen J, 2010, J VIROL, V84, P1289, DOI 10.1128/JVI.01281-09; Chen WJ, 2004, J MED MICROBIOL, V53, P435, DOI 10.1099/jmm.0.45561-0; Chen Z, 2017, J INFECT DIS, V215, P1807, DOI 10.1093/infdis/jix209; Cheng VCC, 2007, CLIN MICROBIOL REV, V20, P660, DOI 10.1128/CMR.00023-07; Chiu YH, 2009, CELL, V138, P576, DOI 10.1016/j.cell.2009.06.015; Civril F, 2011, EMBO REP, V12, P1127, DOI 10.1038/embor.2011.190; Coleman CM, 2014, VACCINE, V32, P3169, DOI 10.1016/j.vaccine.2014.04.016; DAHER KA, 1986, J VIROL, V60, P1068, DOI 10.1128/JVI.60.3.1068-1074.1986; Davis BK, 2011, J IMMUNOL, V186, P1333, DOI 10.4049/jimmunol.1003111; Dutzan N, 2019, ADV EXP MED BIOL, V1197, P107, DOI 10.1007/978-3-030-28524-1_9; Ermolaeva MA, 2008, NAT IMMUNOL, V9, P1037, DOI 10.1038/ni.1638; Fairman P, 2012, CLIN EXP IMMUNOL, V170, P101, DOI 10.1111/j.1365-2249.2012.04628.x; Gallego-Marin C, 2018, J IMMUNOL, V200, P768, DOI 10.4049/jimmunol.1701048; Geijtenbeek TBH, 2009, NAT REV IMMUNOL, V9, P465, DOI 10.1038/nri2569; Gordon S, 2002, CELL, V111, P927, DOI 10.1016/S0092-8674(02)01201-1; Goubau D, 2014, NATURE, V514, P372, DOI 10.1038/nature13590; Gralinski LE, 2018, MBIO, V9, DOI 10.1128/mBio.01753-18; Guo Y, 2012, PLOS ONE, V7, DOI 10.1371/journal.pone.0040179; Hacker H, 2006, NATURE, V439, P204, DOI 10.1038/nature04369; Hacker Hans, 2006, Sci STKE, V2006, pre13, DOI 10.1126/stke.3572006re13; Hara H, 2013, NAT IMMUNOL, V14, P1247, DOI 10.1038/ni.2749; Hemmi H, 2000, NATURE, V408, P740, DOI 10.1038/35047123; Hornung V, 2009, NATURE, V458, P514, DOI 10.1038/nature07725; Hou J, 2009, J IMMUNOL, V183, P2150, DOI 10.4049/jimmunol.0900707; Houser KV, 2016, J INFECT DIS, V213, P1557, DOI 10.1093/infdis/jiw080; Inohara N, 2005, ANNU REV BIOCHEM, V74, P355, DOI 10.1146/annurev.biochem.74.082803.133347; Inohara N, 2001, ONCOGENE, V20, P6473, DOI 10.1038/sj.onc.1204787; Ivashkiv LB, 2014, NAT REV IMMUNOL, V14, P36, DOI 10.1038/nri3581; Jensen S, 2012, J VIROL, V86, P2900, DOI 10.1128/JVI.05738-11; Kaewraemruaen Chamraj, 2019, Asian Pac J Allergy Immunol, DOI 10.12932/AP-070919-0639; Kawai T, 2010, NAT IMMUNOL, V11, P373, DOI 10.1038/ni.1863; Kell AM, 2015, VIROLOGY, V479, P110, DOI 10.1016/j.virol.2015.02.017; Koh D, 2010, ASIA-PAC J PUBLIC HE, V22, p132S, DOI 10.1177/1010539510373010; Kopecky-Bromberg SA, 2007, J VIROL, V81, P548, DOI 10.1128/JVI.01782-06; LEHRER RI, 1993, ANNU REV IMMUNOL, V11, P105, DOI 10.1146/annurev.iy.11.040193.000541; Li SZ, 2019, NAT COMMUN, V10, DOI 10.1038/s41467-019-11258-x; Liu Y, 2014, VIRAL IMMUNOL, V27, P267, DOI 10.1089/vim.2013.0127; Lu XL, 2011, VIRUS GENES, V42, P37, DOI 10.1007/s11262-010-0544-x; Ma DY, 2015, CURR OPIN VIROL, V12, P26, DOI 10.1016/j.coviro.2015.02.005; Maloir Q, 2018, Rev Med Liege, V73, P370; Manni ML, 2014, EXPERT REV RESP MED, V8, P25, DOI 10.1586/17476348.2014.854167; Mubarak A, 2019, J IMMUNOL RES, DOI 10.1155/2019/6491738; NAKASHIMA H, 1993, AIDS, V7, P1129, DOI 10.1097/00002030-199308000-00019; Nelemans T, 2019, VIRUSES-BASEL, V11, DOI 10.3390/v11100961; Ng OW, 2016, VACCINE, V34, P2008, DOI 10.1016/j.vaccine.2016.02.063; Niu PH, 2018, SCI CHINA LIFE SCI, V61, P1280, DOI 10.1007/s11427-018-9343-8; Niu PH, 2018, J INFECT DIS, V218, P1249, DOI 10.1093/infdis/jiy311; Nouri-Shirazi M, 2012, J IMMUNOL, V188, P2359, DOI 10.4049/jimmunol.1102552; Okumura A, 2010, J VIROL, V84, P27, DOI 10.1128/JVI.01462-09; Pascal KE, 2015, P NATL ACAD SCI USA, V112, P8738, DOI 10.1073/pnas.1510830112; Pauli EK, 2008, PLOS PATHOG, V4, DOI 10.1371/journal.ppat.1000196; Phadwal K, 2012, AUTOPHAGY, V8, P677, DOI 10.4161/auto.18935; Pobezinskaya YL, 2008, NAT IMMUNOL, V9, P1047, DOI 10.1038/ni.1639; Rogers NC, 2005, IMMUNITY, V22, P507, DOI 10.1016/j.immuni.2005.03.004; Sato K, 2018, J VIROL, V92, DOI 10.1128/JVI.01905-17; Schneider WM, 2014, ANNU REV IMMUNOL, V32, P513, DOI 10.1146/annurev-immunol-032713-120231; Seranova Elena, 2019, Methods Mol Biol, V1880, P389, DOI 10.1007/978-1-4939-8873-0_26; Spiegel M, 2005, J VIROL, V79, P2079, DOI 10.1128/JVI.79.4.2079-2086.2005; Strasser D, 2012, IMMUNITY, V36, P32, DOI 10.1016/j.immuni.2011.11.015; Sun LJ, 2013, SCIENCE, V339, P786, DOI 10.1126/science.1232458; Sun SH, 2013, AM J RESP CELL MOL, V49, P221, DOI 10.1165/rcmb.2012-0428OC; Takahasi K, 2009, J BIOL CHEM, V284, P17465, DOI 10.1074/jbc.M109.007179; Takeuchi O, 2010, CELL, V140, P805, DOI 10.1016/j.cell.2010.01.022; Tu ZK, 2012, INT IMMUNOL, V24, P97, DOI 10.1093/intimm/dxr104; van Doremalen N, 2017, ANTIVIR RES, V143, P30, DOI 10.1016/j.antiviral.2017.03.025; Van Gorp H, 2014, FEBS J, V281, P4568, DOI 10.1111/febs.13014; Weber M, 2013, CELL HOST MICROBE, V13, P336, DOI 10.1016/j.chom.2013.01.012; Weingartl H, 2004, J VIROL, V78, P12672, DOI 10.1128/JVI.78.22.12672-12676.2004; White MR, 2007, AM J PHYSIOL-LUNG C, V293, pL1293, DOI 10.1152/ajplung.00266.2007; World Health Organization, LAB TEST HUM SUSP CA; World Health Organization, 2 WHO; World Health Organization, WHO MERS GLOB SUMM A; World Health Organization, MIDDL E RESP SYNDR C; Wu B, 2013, CELL, V152, P276, DOI 10.1016/j.cell.2012.11.048; Wu L, 2004, CELL MOL IMMUNOL, V1, P112; Yang Y, 2005, BIOCHEM J, V392, P135, DOI 10.1042/BJ20050698; Yasui F, 2008, J IMMUNOL, V181, P6337, DOI 10.4049/jimmunol.181.9.6337; Ying TL, 2014, J VIROL, V88, P7796, DOI 10.1128/JVI.00912-14; Yoneyama M, 2015, CURR OPIN IMMUNOL, V32, P48, DOI 10.1016/j.coi.2014.12.012; Yoneyama M, 2010, REV MED VIROL, V20, P4, DOI 10.1002/rmv.633; Yoneyama M, 2009, IMMUNOL REV, V227, P54, DOI 10.1111/j.1600-065X.2008.00727.x; Zapata W, 2016, MICROBES INFECT, V18, P211, DOI 10.1016/j.micinf.2015.10.009; Zhao JC, 2014, P NATL ACAD SCI USA, V111, P4970, DOI 10.1073/pnas.1323279111; Zharkova MS, 2019, FRONT CELL INFECT MI, V9, DOI 10.3389/fcimb.2019.00128; Zhu XH, 2011, PLOS ONE, V6, DOI 10.1371/journal.pone.0023730	98	4	4	51	51	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0146-6615	1096-9071		J MED VIROL	J. Med. Virol.	APR	2020	92	4			SI		424	432		10.1002/jmv.25685		FEB 2020	9	Virology	Virology	KM6QU	WOS:000511535900001	31981224	Bronze			2020-04-01	
J	Ceraolo, C; Giorgi, FM				Ceraolo, Carmine; Giorgi, Federico M.			Genomic variance of the 2019-nCoV coronavirus	JOURNAL OF MEDICAL VIROLOGY			English	Article						biostatistics & bioinformatics; CLUSTAL analysis; coronavirus; data visualization; virus classification	MULTIPLE SEQUENCE ALIGNMENT	There is a rising global concern for the recently emerged novel coronavirus (2019-nCoV). Full genomic sequences have been released by the worldwide scientific community in the last few weeks to understand the evolutionary origin and molecular characteristics of this virus. Taking advantage of all the genomic information currently available, we constructed a phylogenetic tree including also representatives of other coronaviridae, such as Bat coronavirus (BCoV) and severe acute respiratory syndrome. We confirm high sequence similarity (>99%) between all sequenced 2019-nCoVs genomes available, with the closest BCoV sequence sharing 96.2% sequence identity, confirming the notion of a zoonotic origin of 2019-nCoV. Despite the low heterogeneity of the 2019-nCoV genomes, we could identify at least two hypervariable genomic hotspots, one of which is responsible for a Serine/Leucine variation in the viral ORF8-encoded protein. Finally, we perform a full proteomic comparison with other coronaviridae, identifying key aminoacidic differences to be considered for antiviral strategies deriving from previous anti-coronavirus approaches.	[Ceraolo, Carmine; Giorgi, Federico M.] Univ Bologna, Dept Pharm & Biotechnol, Via Selmi 3, I-40126 Bologna, Italy	Giorgi, FM (reprint author), Univ Bologna, Dept Pharm & Biotechnol, Via Selmi 3, I-40126 Bologna, Italy.	federico.giorgi@unibo.it	Ribeiro, Nuno/AAH-2299-2020; Giorgi, Federico M./AAE-4281-2020	Giorgi, Federico M./0000-0002-7325-9908	Ministero dell'Istruzione, dell' Universita e della RicercaMinistero dell' Istruzione, dell' Universita e della Ricerca (MIUR)	Ministero dell'Istruzione, dell' Universita e della Ricerca, Grant/Award Number: Montalcini 2016	ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1006/jmbi.1990.9999; Anand K, 2003, SCIENCE, V300, P1763, DOI 10.1126/science.1085658; Benvenuto D, 2020, J MED VIROL, V92, P455, DOI 10.1002/jmv.25688; Burley Stephen K, 2017, Methods Mol Biol, V1607, P627, DOI 10.1007/978-1-4939-7000-1_26; Cui J, 2019, NAT REV MICROBIOL, V17, P181, DOI 10.1038/s41579-018-0118-9; Edgar RC, 2004, NUCLEIC ACIDS RES, V32, P1792, DOI 10.1093/nar/gkh340; FELSENSTEIN J, 1985, EVOLUTION, V39, P783, DOI 10.1111/j.1558-5646.1985.tb00420.x; Hu B, 2015, VIROL J, V12, DOI 10.1186/s12985-015-0422-1; Kumar S, 2018, MOL BIOL EVOL, V35, P1547, DOI 10.1093/molbev/msy096; Le S, 2008, J STAT SOFTW, V25, P1, DOI 10.18637/jss.v025.i01; Linding R, 2003, NUCLEIC ACIDS RES, V31, P3701, DOI 10.1093/nar/gkg519; Lu HZ, 2020, J MED VIROL, V92, P401, DOI 10.1002/jmv.25678; Lu RJ, 2020, LANCET, V395, P565, DOI 10.1016/S0140-6736(20)30251-8; Manaresi E, 2017, VIROLOGY, V508, P54, DOI 10.1016/j.virol.2017.05.006; Narayanan K, 2008, VIRUS RES, V133, P113, DOI 10.1016/j.virusres.2007.10.009; Sayers EW, 2019, NUCLEIC ACIDS RES, V47, pD94, DOI 10.1093/nar/gky989; Schoeman D, 2019, VIROL J, V16, DOI 10.1186/s12985-019-1182-0; Shu YL, 2017, EUROSURVEILLANCE, V22, P2, DOI 10.2807/1560-7917.ES.2017.22.13.30494; TAMURA K, 1993, MOL BIOL EVOL, V10, P512; Waterhouse AM, 2009, BIOINFORMATICS, V25, P1189, DOI 10.1093/bioinformatics/btp033	20	0	0	48	48	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0146-6615	1096-9071		J MED VIROL	J. Med. Virol.	MAY	2020	92	5					522	528		10.1002/jmv.25700		FEB 2020	7	Virology	Virology	KU3AY	WOS:000517439500001	32027036	Bronze			2020-04-01	
J	Eickmann, M; Gravemann, U; Handke, W; Tolksdorf, F; Reichenberg, S; Muller, TH; Seltsam, A				Eickmann, Markus; Gravemann, Ute; Handke, Wiebke; Tolksdorf, Frank; Reichenberg, Stefan; Mueller, Thomas H.; Seltsam, Axel			Inactivation of three emerging viruses - severe acute respiratory syndrome coronavirus, Crimean-Congo haemorrhagic fever virus and Nipah virus - in platelet concentrates by ultraviolet C light and in plasma by methylene blue plus visible light	VOX SANGUINIS			English	Article; Early Access						ultraviolet light; methylene blue; pathogen inactivation; plasma; platelet concentrates	FRESH-FROZEN PLASMA; PATHOGEN INACTIVATION; REDUCTION; SYSTEM; EPIDEMIOLOGY; TRANSMISSION; INFECTIVITY	Background Emerging viruses like severe acute respiratory syndrome coronavirus (SARS-CoV), Crimean-Congo haemorrhagic fever virus (CCHFV) and Nipah virus (NiV) have been identified to pose a potential threat to transfusion safety. In this study, the ability of the THERAFLEX UV-Platelets and THERAFLEX MB-Plasma pathogen inactivation systems to inactivate these viruses in platelet concentrates and plasma, respectively, was investigated. Materials and methods Blood products were spiked with SARS-CoV, CCHFV or NiV, and then treated with increasing doses of UVC light (THERAFLEX UV-Platelets) or with methylene blue (MB) plus increasing doses of visible light (MB/light; THERAFLEX MB-Plasma). Samples were taken before and after treatment with each illumination dose and tested for residual infectivity. Results Treatment with half to three-fourths of the full UVC dose (0 center dot 2 J/cm(2)) reduced the infectivity of SARS-CoV (>= 3 center dot 4 log), CCHFV (>= 2 center dot 2 log) and NiV (>= 4 center dot 3 log) to the limit of detection (LOD) in platelet concentrates, and treatment with MB and a fourth of the full light dose (120 J/cm(2)) decreased that of SARS-CoV (>= 3 center dot 1 log), CCHFV (>= 3 center dot 2 log) and NiV (>= 2 center dot 7 log) to the LOD in plasma. Conclusion Our study demonstrates that both THERAFLEX UV-Platelets (UVC) and THERAFLEX MB-Plasma (MB/light) effectively reduce the infectivity of SARS-CoV, CCHFV and NiV in platelet concentrates and plasma, respectively.	[Eickmann, Markus] Philipps Univ Marburg, Inst Virol, Marburg, Germany; [Gravemann, Ute; Handke, Wiebke; Mueller, Thomas H.; Seltsam, Axel] German Red Cross Blood Serv NSTOB, Springe, Germany; [Tolksdorf, Frank; Reichenberg, Stefan] Maco Pharma Int GmbH, Langen, Germany	Seltsam, A (reprint author), German Red Cross Blood Serv NSTOB, Inst Springe, Eldagsener Str 38, D-31832 Springe, Germany.	axel.seltsam@bsd-nstob.de	Reichenberg, Stefan/AAB-3159-2020	Muller, Thomas H./0000-0002-5417-6787	German Red Cross Blood Services (Deutsche Forschungsgemeinschaft der Blutspendedienste des Deutschen Roten Kreuzes); Macopharma S.A.S.	This work was supported by the German Red Cross Blood Services (Deutsche Forschungsgemeinschaft der Blutspendedienste des Deutschen Roten Kreuzes) and Macopharma S.A.S.	Agafonov A P, 2004, Dokl Biol Sci, V394, P58, DOI 10.1023/B:DOBS.0000017131.06970.74; Ang BSP, 2018, J CLIN MICROBIOL, V56, DOI 10.1128/JCM.01875-17; ARNASON NA, 2019, [No title captured], DOI DOI 10.1111/TRF.15575; Bahar B, 2020, BRIT J HAEMATOL, V188, P465, DOI 10.1111/bjh.16187; Bente DA, 2013, ANTIVIR RES, V100, P159, DOI 10.1016/j.antiviral.2013.07.006; Castro E, 2008, VOX SANG, V95, P290; Castro E, 2014, TRANSFUSION, V54, P2207, DOI 10.1111/trf.12598; Conger NG, 2015, EMERG INFECT DIS, V21, P23, DOI 10.3201/eid2101.141413; de Wit E, 2016, NAT REV MICROBIOL, V14, P523, DOI 10.1038/nrmicro.2016.81; Dunstan RA, 2008, AUST NZ J PUBL HEAL, V32, P354, DOI 10.1111/j.1753-6405.2008.00254.x; Eickmann M, 2018, TRANSFUSION, V58, P2202, DOI 10.1111/trf.14652; Faddy HM, 2016, TRANSFUSION, V56, P1548, DOI 10.1111/trf.13519; Fryk JJ, 2017, TRANSFUSION, V57, P2677, DOI 10.1111/trf.14256; Gravemann U, 2019, TRANSFUSION, V59, P1324, DOI 10.1111/trf.15119; Gravemann U, 2018, TRANSFUSION, V58, P2669, DOI 10.1111/trf.14957; Karber G, 1931, N-S ARCH EX PATH PH, V162, P480, DOI 10.1007/BF01863914; Kim S, 2018, TRANSFUSION, V58, P758, DOI 10.1111/trf.14464; McCullough J, 2019, TRANSFUSION, V59, P1132, DOI 10.1111/trf.15103; MOHR H, 1995, IMMUNOL INVEST, V24, P73, DOI 10.3109/08820139509062763; Mohr H, 2009, TRANSFUSION, V49, P2612, DOI 10.1111/j.1537-2995.2009.02334.x; Nikolay B, 2019, NEW ENGL J MED, V380, P1804, DOI 10.1056/NEJMoa1805376; Olschlager S, 2011, J CLIN VIROL, V50, P90, DOI 10.1016/j.jcv.2010.09.018; Politis C, 2014, TRANSFUSION MED, V24, P316, DOI 10.1111/tme.12144; Seghatchian J, 2008, TRANSFUS APHER SCI, V38, P271, DOI 10.1016/j.transci.2008.04.004; Seghatchian J, 2011, TRANSFUS MED HEMOTH, V38, P55, DOI 10.1159/000323786; Seltsam A, 2013, BRIT J HAEMATOL, V162, P442, DOI 10.1111/bjh.12403; Seltsam A, 2011, TRANSFUS MED HEMOTH, V38, P43, DOI 10.1159/000323845; Sharma V, 2019, REV MED VIROL, V29, DOI 10.1002/rmv.2010; Spearman C, 1908, BRIT J PSYCHOL, V2, P227, DOI 10.1111/j.2044-8295.1908.tb00176.x; Stramer SL, 2014, ISBT SCI SER, V9, P30, DOI 10.1111/voxs.12070; Stramer SL, 2009, TRANSFUSION, V49, p1S, DOI 10.1111/j.1537-2995.2009.02279.x; Wild TF, 2009, PATHOL BIOL, V57, P188, DOI 10.1016/j.patbio.2008.04.006; Williamson LM, 2003, TRANSFUSION, V43, P1322, DOI 10.1046/j.1537-2995.2003.00483.x; Woolhouse MEJ, 2005, TRENDS ECOL EVOL, V20, P238, DOI 10.1016/j.tree.2005.02.009	34	0	0	48	48	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0042-9007	1423-0410		VOX SANG	Vox Sang.												10.1111/vox.12888		JAN 2020	6	Hematology	Hematology	KB7BT	WOS:000506646700001	31930543	Bronze			2020-04-01	
J	Arabi, YM; Murthy, S; Webb, S				Arabi, Yaseen M.; Murthy, Srinivas; Webb, Steve			COVID-19: a novel coronavirus and a novel challenge for critical care	INTENSIVE CARE MEDICINE			English	Editorial Material; Early Access							EAST RESPIRATORY SYNDROME; ILL PATIENTS; OUTBREAK		[Arabi, Yaseen M.] Minist Natl Guard Hlth Affairs, Intens Care Dept, Riyadh, Saudi Arabia; [Arabi, Yaseen M.] King Abdullah Int Med Res Ctr, Riyadh, Saudi Arabia; [Arabi, Yaseen M.] King Saud Bin Abdulaziz Univ Hlth Sci, ICU 1425,POB 22490, Riyadh 11426, Saudi Arabia; [Murthy, Srinivas] Univ British Columbia, Vancouver, BC V6H 3V4, Canada; [Webb, Steve] Monash Univ, Sch Publ Hlth & Prevent Med, Melbourne, Vic, Australia	Arabi, YM (reprint author), Minist Natl Guard Hlth Affairs, Intens Care Dept, Riyadh, Saudi Arabia.	arabi@ngha.med.sa		Arabi, Yaseen/0000-0001-5735-6241			Adaptive Platform Trials Coalition, 2019, Nat Rev Drug Discov, V18, P797, DOI 10.1038/s41573-019-0034-3; Al-Dorzi HM, 2016, ANN INTENSIVE CARE, V6, DOI 10.1186/s13613-016-0203-z; Almutairi AF, 2018, J INFECT PUBLIC HEAL, V11, P187, DOI 10.1016/j.jiph.2017.06.011; Arabi YM, 2017, CRIT CARE MED, V45, P1683, DOI 10.1097/CCM.0000000000002621; Booth Christopher M, 2005, Crit Care Med, V33, pS53, DOI 10.1097/01.CCM.0000150954.88817.6; Brun-Buisson C, 2003, INTENS CARE MED, V29, P861, DOI 10.1007/s00134-003-1823-y; Chan JFW, 2020, LANCET, V395, P514, DOI 10.1016/S0140-6736(20)30154-9; Chen NS, 2020, LANCET, V395, P507, DOI 10.1016/S0140-6736(20)30211-7; Fowler RA, 2003, JAMA-J AM MED ASSOC, V290, P367, DOI 10.1001/jama.290.3.367; Hick JL, 2014, CHEST, V146, pE1S, DOI 10.1378/chest.14-0733; Huang CL, 2020, LANCET, V395, P497, DOI 10.1016/S0140-6736(20)30183-5; Iwasenko JM, 2010, CLIN INFECT DIS, V51, P70, DOI 10.1086/653610; Li Q, 2020, NEW ENGL J MED, DOI [10.1056/NEJMoa2002032, DOI 10.1056/NEJMOA2002032]; Lu RJ, 2020, LANCET, V395, P565, DOI 10.1016/S0140-6736(20)30251-8; Lurie N, 2013, NEW ENGL J MED, V368, P1251, DOI 10.1056/NEJMsb1209510; Munster VJ, 2020, NEW ENGL J MED, V382, P692, DOI 10.1056/NEJMp2000929; Perlman S, 2020, NEW ENGL J MED, V382, P760, DOI 10.1056/NEJMe2001126; Shalhoub S, 2018, PLOS ONE, V13, DOI 10.1371/journal.pone.0206831; Wang C, 2020, LANCET, V395, P470, DOI 10.1016/S0140-6736(20)30185-9; Wang Dawei, 2020, JAMA, DOI 10.1001/jama.2020.1585; World Health Organization, 2019, 9 WHO; Yang Xiaobo, 2020, Lancet Respir Med, DOI 10.1016/S2213-2600(20)30079-5; Zhou P, 2020, NATURE, DOI 10.1038/s41586-020-2012-7; Zhu N, 2020, NEW ENGL J MED, V382, P727, DOI 10.1056/NEJMoa2001017	24	0	0	47	47	SPRINGER	NEW YORK	ONE NEW YORK PLAZA, SUITE 4600, NEW YORK, NY, UNITED STATES	0342-4642	1432-1238		INTENS CARE MED	Intensive Care Med.												10.1007/s00134-020-05955-1		MAR 2020	4	Critical Care Medicine	General & Internal Medicine	KS1EV	WOS:000518054400005	32125458	Bronze			2020-04-01	
J	Li, XG; Wang, W; Zhao, XF; Zai, JJ; Zhao, Q; Li, Y; Chaillon, A				Li, Xingguang; Wang, Wei; Zhao, Xiaofang; Zai, Junjie; Zhao, Qiang; Li, Yi; Chaillon, Antoine			Transmission dynamics and evolutionary history of 2019-nCoV	JOURNAL OF MEDICAL VIROLOGY			English	Article						2019-nCoV; evolutionary rate; phylogenetic cluster; time to most recent common ancestor; TMRCA; transmission cluster	RESPIRATORY SYNDROME CORONAVIRUS; SARS CORONAVIRUS; RECOMBINATION; EPIDEMIOLOGY; PERFORMANCE; ALGORITHMS; INFECTION; CAMELS; GENOME; CHINA	To investigate the time origin, genetic diversity, and transmission dynamics of the recent 2019-nCoV outbreak in China and beyond, a total of 32 genomes of virus strains sampled from China, Thailand, and the USA with sampling dates between 24 December 2019 and 23 January 2020 were analyzed. Phylogenetic, transmission network, and likelihood-mapping analyses of the genome sequences were performed. On the basis of the likelihood-mapping analysis, the increasing tree-like signals (from 0% to 8.2%, 18.2%, and 25.4%) over time may be indicative of increasing genetic diversity of 2019-nCoV in human hosts. We identified three phylogenetic clusters using the Bayesian inference framework and three transmission clusters using transmission network analysis, with only one cluster identified by both methods using the above genome sequences of 2019-nCoV strains. The estimated mean evolutionary rate for 2019-nCoV ranged from 1.7926 x 10(-3) to 1.8266 x 10(-3) substitutions per site per year. On the basis of our study, undertaking epidemiological investigations and genomic data surveillance could positively impact public health in terms of guiding prevention efforts to reduce 2019-nCOV transmission in real-time.	[Li, Xingguang; Li, Yi] Wuhan Univ Bioengn, Hubei Engn Res Ctr Viral Vector, Wuhan 430415, Peoples R China; [Wang, Wei] Guangxi Univ Chinese Med, Dept Spleen & Stomach Dis, Affiliated Hosp 1, Nanning, Peoples R China; [Zhao, Xiaofang] Guangxi Univ Chinese Med, Dept Sci & Technol, Ruikang Hosp, Nanning, Peoples R China; [Zai, Junjie] Ningbo Univ, Sch Med, Immunol Innovat Team, Ningbo, Peoples R China; [Zhao, Qiang] Tianjin Canc Hosp, Precis Canc Ctr, Airport Ctr, Airport Hosp, Tianjin, Peoples R China; [Chaillon, Antoine] Univ Calif San Diego, Dept Med, La Jolla, CA 92093 USA	Li, XG; Li, Y (reprint author), Wuhan Univ Bioengn, Hubei Engn Res Ctr Viral Vector, Wuhan 430415, Peoples R China.; Chaillon, A (reprint author), Univ Calif San Diego, Dept Med, La Jolla, CA 92093 USA.	xingguanglee@hotmail.com; liyi@whsw.edu.cn; achaillon@health.ucsd.edu	Ribeiro, Nuno/AAH-2299-2020		National Natural Science Foundation of ChinaNational Natural Science Foundation of China [31470268]; Project of Guangxi Health Committee [Z20191111]; Natural Science Foundation of Guangxi Province of ChinaNational Natural Science Foundation of Guangxi Province [2017GXNSFAA198080]	National Natural Science Foundation of China, Grant/Award Number: 31470268; Project of Guangxi Health Committee, Grant/Award Number: Z20191111; Natural Science Foundation of Guangxi Province of China, Grant/Award Number: 2017GXNSFAA198080	Chan JFW, 2020, LANCET, V395, P514, DOI 10.1016/S0140-6736(20)30154-9; CHINESE SME, 2004, SCIENCE, V303, P1666, DOI DOI 10.1126/SCIENCE.1092002; Chu DKW, 2014, EMERG INFECT DIS, V20, P1049, DOI 10.3201/eid2006.140299; Cotten M, 2014, MBIO, V5, DOI 10.1128/mBio.01062-13; Cotten M, 2013, LANCET, V382, P1993, DOI 10.1016/S0140-6736(13)61887-5; Darriba D, 2012, NAT METHODS, V9, P772, DOI 10.1038/nmeth.2109; de Groot RJ, 2013, J VIROL, V87, P7790, DOI 10.1128/JVI.01244-13; Drosten C, 2003, NEW ENGL J MED, V348, P1967, DOI 10.1056/NEJMoa030747; Drummond AJ, 2012, MOL BIOL EVOL, V29, P1969, DOI 10.1093/molbev/mss075; Elbe S, 2017, GLOB CHALL, V1, P33, DOI 10.1002/gch2.1018; Guan Y, 2003, SCIENCE, V302, P276, DOI 10.1126/science.1087139; Guindon S, 2010, SYST BIOL, V59, P307, DOI 10.1093/sysbio/syq010; Hall T.A., 1999, NUCL ACIDS S SER, V41, P95, DOI DOI 10.1021/BK-1999-0734.CH008; Huson DH, 2006, MOL BIOL EVOL, V23, P254, DOI 10.1093/molbev/msj030; Katoh K, 2013, MOL BIOL EVOL, V30, P772, DOI 10.1093/molbev/mst010; Ksiazek TG, 2003, NEW ENGL J MED, V348, P1953, DOI 10.1056/NEJMoa030781; Lau SKP, 2005, P NATL ACAD SCI USA, V102, P14040, DOI 10.1073/pnas.0506735102; Lau SKP, 2010, J VIROL, V84, P2808, DOI 10.1128/JVI.02219-09; Li Q, 2020, NEW ENGL J MED, DOI [10.1056/NEJMoa2001316, DOI 10.1056/NEJM0A2001316]; Li WD, 2005, SCIENCE, V310, P676, DOI 10.1126/science.1118391; Li XG, 2020, J MED VIROL, V92, P448, DOI 10.1002/jmv.25693; Lu R, 2020, LANCET; Muller MA, 2014, EMERG INFECT DIS, V20, P2093, DOI 10.3201/eid2012.141026; Muth D, 2018, SCI REP-UK, V8, DOI 10.1038/s41598-018-33487-8; Pond SLK, 2018, MOL BIOL EVOL, V35, P1812, DOI 10.1093/molbev/msy016; Rambaut A, 2018, SYST BIOL, V67, P901, DOI 10.1093/sysbio/syy032; Rambaut A, 2016, VIRUS EVOL, V2, DOI 10.1093/ve/vew007; Schmidt HA, 2002, BIOINFORMATICS, V18, P502, DOI 10.1093/bioinformatics/18.3.502; Schmidt HA, 2007, CURR PROTOC BIOINFOR, V1, P661; Song HD, 2005, P NATL ACAD SCI USA, V102, P2430, DOI 10.1073/pnas.0409608102; Su S, 2016, TRENDS MICROBIOL, V24, P490, DOI 10.1016/j.tim.2016.03.003; Suchard MA, 2009, BIOINFORMATICS, V25, P1370, DOI 10.1093/bioinformatics/btp244; Wang M, 2005, EMERG INFECT DIS, V11, P1860, DOI 10.3201/eid1112.041293; Zaki AM, 2012, NEW ENGL J MED, V367, P1814, DOI 10.1056/NEJMoa1211721; Zhao ZM, 2004, BMC EVOL BIOL, V4, DOI 10.1186/1471-2148-4-21; Zhong NS, 2003, LANCET, V362, P1353, DOI 10.1016/S0140-6736(03)14630-2; Zhou P, 2020, DISCOVERY NOVEL CORO	37	0	0	47	47	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0146-6615	1096-9071		J MED VIROL	J. Med. Virol.	MAY	2020	92	5					501	511		10.1002/jmv.25701		FEB 2020	11	Virology	Virology	KU3AY	WOS:000513293400001	32027035	Bronze			2020-04-01	
J	Xia, S; Zhu, Y; Liu, MQ; Lan, QH; Xu, W; Wu, YL; Ying, TL; Liu, SW; Shi, ZL; Jiang, SB; Lu, L				Xia, Shuai; Zhu, Yun; Liu, Meiqin; Lan, Qiaoshuai; Xu, Wei; Wu, Yanling; Ying, Tianlei; Liu, Shuwen; Shi, Zhengli; Jiang, Shibo; Lu, Lu			Fusion mechanism of 2019-nCoV and fusion inhibitors targeting HR1 domain in spike protein	CELLULAR & MOLECULAR IMMUNOLOGY			English	Letter; Early Access							CORONAVIRUS		[Xia, Shuai; Lan, Qiaoshuai; Xu, Wei; Wu, Yanling; Ying, Tianlei; Jiang, Shibo; Lu, Lu] Fudan Univ, Sch Basic Med Sci, Fudan Jinbo Joint Res Ctr, Key Lab Med Mol Virol MOE NHC CAMS, Shanghai, Peoples R China; [Zhu, Yun] Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing, Peoples R China; [Liu, Meiqin; Shi, Zhengli] Chinese Acad Sci, Wuhan Inst Virol, CAS Key Lab Special Pathogens & Biosafety, Wuhan, Peoples R China; [Liu, Shuwen] Southern Med Univ, Guangzhou Key Lab Drug Res Emerging Virus Prevent, Sch Pharmaceut Sci, Guangdong Prov Key Lab New Drug Screening, Guangzhou, Peoples R China; [Jiang, Shibo] New York Blood Ctr, Lindsley F Kimball Res Inst, New York, NY 10021 USA	Jiang, SB; Lu, L (reprint author), Fudan Univ, Sch Basic Med Sci, Fudan Jinbo Joint Res Ctr, Key Lab Med Mol Virol MOE NHC CAMS, Shanghai, Peoples R China.; Jiang, SB (reprint author), New York Blood Ctr, Lindsley F Kimball Res Inst, New York, NY 10021 USA.	shibojiang@fudan.edu.cn; lul@fudan.edu.cn	Ribeiro, Nuno/AAH-2299-2020	Ying, Tianlei/0000-0002-9597-2843	National Megaprojects of China for Major Infectious Diseases [2018ZX10301403]	We thank Qian Wang at the Core Facility of Microbiology and Parasitology (SHMC) of Fudan University. We thank GL Biochem (Shanghai) Company for peptide synthesis. This work was supported by the National Megaprojects of China for Major Infectious Diseases (2018ZX10301403).	Huang CL, 2020, LANCET, V395, P497, DOI 10.1016/S0140-6736(20)30183-5; Jiang SB, 2020, EMERG MICROBES INFEC, V9, P275, DOI 10.1080/22221751.2020.1723441; Li F, 2005, SCIENCE, V309, P1864, DOI 10.1126/science.1116480; Liu SW, 2004, LANCET, V363, P938, DOI 10.1016/S0140-6736(04)15788-7; Lu L, 2014, NAT COMMUN, V5, DOI 10.1038/ncomms4067; Xia S, 2019, SCI ADV, V5, DOI 10.1126/sciadv.aav4580; Yuan Y, 2017, NAT COMMUN, V8, DOI 10.1038/ncomms15092; Zhou P, 2020, NATURE, DOI 10.1038/s41586-020-2012-7	8	1	1	47	47	NATURE PUBLISHING GROUP	LONDON	MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND	1672-7681	2042-0226		CELL MOL IMMUNOL	Cell. Mol. Immunol.												10.1038/s41423-020-0374-2		FEB 2020	3	Immunology	Immunology	KK6IV	WOS:000512844500004	32047258	Bronze, Green Accepted			2020-04-01	
J	Ayittey, FK; Dzuvor, C; Ayittey, MK; Chiwero, NB; Habib, A				Ayittey, Foster Kofi; Dzuvor, Christian; Ayittey, Matthew Kormla; Chiwero, Nyasha Bennita; Habib, Ahmed			Updates on Wuhan 2019 novel coronavirus epidemic	JOURNAL OF MEDICAL VIROLOGY			English	Editorial Material									[Ayittey, Foster Kofi; Chiwero, Nyasha Bennita] Curtin Univ Malaysia, Dept Chem Engn, CDT 250, Sarawak 98009, Malaysia; [Dzuvor, Christian] Monash Univ, Dept Chem Engn, Clayton, Vic, Australia; [Ayittey, Matthew Kormla] Minist Food & Agr, Agr Extens Off, Adidome, Ghana; [Habib, Ahmed] Ghana Hlth Serv, Urol Div, Sogakope, Ghana	Ayittey, FK (reprint author), Curtin Univ Malaysia, Dept Chem Engn, CDT 250, Sarawak 98009, Malaysia.	Ayittey.FosterKofi@postgrad.curtin.edu.my		Ayittey, Foster Kofi/0000-0003-3129-2053			[Anonymous], REUTERS NEW BEIJING; [Anonymous], CHINA BRIEFING; [Anonymous], THIS WEEK ASIA; [Anonymous], NY TIMES; [Anonymous], 2017, CHINA DAILY; [Anonymous], CNN WORLD; [Anonymous], GUARDIAN; [Anonymous], GLOBAL TIMES; BBC News, BBC NEWS; Centre for Health Protection of the Hong Kong Special Administrative Region Government, 2020, CHP PROV FURTH INF C; Chan JF-W, 2020, LANCET; Chen Y, 2020, J MED VIROL, V92, P418, DOI 10.1002/jmv.25681; Cohen J., 2020, CHINESE RES REVEAL D; Horton R, 2020, LANCET, V395, P322, DOI 10.1016/S0140-6736(20)30212-9; Huang C, 2020, LANCET; Lai MMC, 2001, FIELDS VIROLOGY LIPP, V1163, P1185; Li G, 2020, J MED VIROL, V92, P424, DOI 10.1002/jmv.25685; Lu HZ, 2020, J MED VIROL, V92, P401, DOI 10.1002/jmv.25678; Luo G, 2020, J MED VIROL, V92, P399, DOI 10.1002/jmv.25683; Steenhuysen J, 2020, WUHAN VIRUS GENETIC; Wang C, 2020, LANCET; Wang WE, 2020, J MED VIROL, V92, P441, DOI 10.1002/jmv.25689; Woo PCY, 2012, J VIROL, V86, P3995, DOI 10.1128/JVI.06540-11; World Health Organization, NOV COR 2019 NCOV AD; Wu JT, 2020, LANCET; Zhang NR, 2020, J MED VIROL, V92, P408, DOI 10.1002/jmv.25674	26	3	3	46	46	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0146-6615	1096-9071		J MED VIROL	J. Med. Virol.	APR	2020	92	4			SI		403	407		10.1002/jmv.25695		FEB 2020	5	Virology	Virology	KM6QU	WOS:000512170300001	32017153	Bronze			2020-04-01	
J	Walls, AC; Xiong, XL; Park, YJ; Tortorici, MA; Snijder, J; Quispe, J; Cameroni, E; Gopal, R; Dai, M; Lanzavecchia, A; Zambon, M; Rey, FA; Corti, D; Veesler, D				Walls, Alexandra C.; Xiong, Xiaoli; Park, Young-Jun; Tortorici, M. Alejandra; Snijder, Joost; Quispe, Joel; Cameroni, Elisabetta; Gopal, Robin; Dai, Mian; Lanzavecchia, Antonio; Zambon, Maria; Rey, Felix A.; Corti, Davide; Veesler, David			Unexpected Receptor Functional Mimicry Elucidates Activation of Coronavirus Fusion	CELL			English	Article							RESPIRATORY SYNDROME CORONAVIRUS; HUMAN MONOCLONAL-ANTIBODY; BEAM-INDUCED MOTION; SARS-CORONAVIRUS; SPIKE PROTEIN; MERS-COV; MAXIMUM-LIKELIHOOD; GLYCAN SHIELD; IMMUNOGENICITY; BINDING	Recent outbreaks of severe acute respiratory syndrome and Middle East respiratory syndrome, along with the threat of a future coronavirus-mediated pandemic, underscore the importance of finding ways to combat these viruses. The trimeric spike transmembrane glycoprotein S mediates entry into host cells and is the major target of neutralizing antibodies. To understand the humoral immune response elicited upon natural infections with coronaviruses, we structurally characterized the SARS-CoV and MERS-CoV S glycoproteins in complex with neutralizing antibodies isolated from human survivors. Although the two antibodies studied blocked attachment to the host cell receptor, only the anti-SARS-CoV S antibody triggered fusogenic conformational changes via receptor functional mimicry. These results provide a structural framework for understanding coronavirus neutralization by human antibodies and shed light on activation of coronavirus membrane fusion, which takes place through a receptor-driven ratcheting mechanism.	[Walls, Alexandra C.; Xiong, Xiaoli; Park, Young-Jun; Tortorici, M. Alejandra; Snijder, Joost; Quispe, Joel; Veesler, David] Univ Washington, Dept Biochem, Seattle, WA 98195 USA; [Tortorici, M. Alejandra; Rey, Felix A.] Inst Pasteur, F-75015 Paris, France; [Tortorici, M. Alejandra; Rey, Felix A.] CNRS UMR 3569, Unite Virol Struct, F-75015 Paris, France; [Cameroni, Elisabetta; Corti, Davide] Humabs Biomed SA, Vir Biotechnol, CH-6500 Bellinzona, Switzerland; [Gopal, Robin; Zambon, Maria] Publ Hlth England, Natl Infect Serv, London NW9 5HT, England; [Dai, Mian] Francis Crick Inst, Crick Worldwide Influenza Ctr, 1 Midland Rd, London NW1 1AT, England; [Lanzavecchia, Antonio] Univ Svizzera italiana, Fac Biomed Sci, Inst Res Biomed, CH-6500 Bellinzona, Switzerland	Veesler, D (reprint author), Univ Washington, Dept Biochem, Seattle, WA 98195 USA.	dveesler@uw.edu		Tortorici, M. Alejandra/0000-0002-2260-2577	National Institute of General Medical SciencesUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of General Medical Sciences (NIGMS) [R01GM120553]; National Institute of Allergy and Infectious DiseasesUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of Allergy & Infectious Diseases (NIAID) [HHSN272201700059C]; Pew Biomedical Scholars Award; Burroughs Wellcome FundBurroughs Wellcome Fund; Netherlands Organisation for Scientific ResearchNetherlands Organization for Scientific Research (NWO) [Rubicon 019.2015.2.310.006]; European Molecular Biology OrganizationEuropean Molecular Biology Organization (EMBO) [ALTF933-2015]; Zoonoses Anticipation and Preparedness Initiative [IMI115760]; Pasteur Institute; Centre National de la Recherche ScientifiqueCentre National de la Recherche Scientifique (CNRS); LabEx Integrative Biology of Emerging Infectious Diseases; University of Washington Arnold and Mabel Beckman CryoEM Center and Proteomics Resource [UWPR95794]; beamline 5.0.1 at the Advanced Light Source at Lawrence Berkley National Laboratory	We are grateful to Thomas Hinds and Ning Zheng for assistance with the crystallization robot and BLI device; to Jerome Cattin-Ortola, Irini Topalidou, and Michael Ailion for assistance with western blotting; and to Michelle Chen (Wyatt technology) for her help with the light scattering experiments. This study was supported by the National Institute of General Medical Sciences (R01GM120553, D.V.), the National Institute of Allergy and Infectious Diseases (HHSN272201700059C, D.V.), a Pew Biomedical Scholars Award (D.V.), an Investigators in the Pathogenesis of Infectious Disease Award from the Burroughs Wellcome Fund (D.V.), the Netherlands Organisation for Scientific Research (Rubicon 019.2015.2.310.006, J.S.), the European Molecular Biology Organization (ALTF933-2015, J.S.), the Zoonoses Anticipation and Preparedness Initiative (IMI115760, F.A.R.), the Pasteur Institute (M.A.T. and F.A.R.), the Centre National de la Recherche Scientifique (F.A.R.), the LabEx Integrative Biology of Emerging Infectious Diseases (F.A.R.), the University of Washington Arnold and Mabel Beckman CryoEM Center and Proteomics Resource (UWPR95794), and the beamline 5.0.1 at the Advanced Light Source at Lawrence Berkley National Laboratory.	Adams PD, 2010, ACTA CRYSTALLOGR D, V66, P213, DOI 10.1107/S0907444909052925; Agirre J, 2015, NAT STRUCT MOL BIOL, V22, P833, DOI 10.1038/nsmb.3115; Belouzard S, 2009, P NATL ACAD SCI USA, V106, P5871, DOI 10.1073/pnas.0809524106; Bern Marshall, 2012, Curr Protoc Bioinformatics, VChapter 13, DOI 10.1002/0471250953.bi1320s40; Blanc E, 2004, ACTA CRYSTALLOGR D, V60, P2210, DOI 10.1107/S0907444904016427; Brown A, 2015, ACTA CRYSTALLOGR D, V71, P136, DOI 10.1107/S1399004714021683; Burkard C, 2014, PLOS PATHOG, V10, DOI 10.1371/journal.ppat.1004502; Chen SX, 2013, ULTRAMICROSCOPY, V135, P24, DOI 10.1016/j.ultramic.2013.06.004; Chen VB, 2010, ACTA CRYSTALLOGR D, V66, P12, DOI 10.1107/S0907444909042073; Corti D, 2015, P NATL ACAD SCI USA, V112, P10473, DOI 10.1073/pnas.1510199112; Cowtan K, 2006, ACTA CRYSTALLOGR D, V62, P1002, DOI 10.1107/S0907444906022116; Deng XF, 2017, J VIROL, V91, DOI 10.1128/JVI.01533-17; Du LY, 2016, NAT COMMUN, V7, DOI 10.1038/ncomms13473; Emsley P, 2010, ACTA CRYSTALLOGR D, V66, P486, DOI 10.1107/S0907444910007493; Evans PR, 2013, ACTA CRYSTALLOGR D, V69, P1204, DOI 10.1107/S0907444913000061; Frenz B, 2019, STRUCTURE, V27, P134, DOI 10.1016/j.str.2018.09.006; Frese CK, 2013, J PROTEOME RES, V12, P1520, DOI 10.1021/pr301130k; Ge XY, 2013, NATURE, V503, P535, DOI 10.1038/nature12711; Goddard TD, 2007, J STRUCT BIOL, V157, P281, DOI 10.1016/j.jsb.2006.06.010; Goddard TD, 2018, PROTEIN SCI, V27, P14, DOI 10.1002/pro.3235; Gui M, 2017, CELL RES, V27, P119, DOI 10.1038/cr.2016.152; Haagmans BL, 2014, LANCET INFECT DIS, V14, P140, DOI 10.1016/S1473-3099(13)70690-X; Hu B, 2017, PLOS PATHOG, V13, DOI 10.1371/journal.ppat.1006698; Kabsch W, 2010, ACTA CRYSTALLOGR D, V66, P125, DOI 10.1107/S0907444909047337; Kimanius D, 2016, ELIFE, V5, DOI 10.7554/eLife.18722; Kirchdoerfer RN, 2018, SCI REP-UK, V8, DOI 10.1038/s41598-018-34171-7; Kirchdoerfer RN, 2016, NATURE, V531, P118, DOI 10.1038/nature17200; Krissinel E, 2007, J MOL BIOL, V372, P774, DOI 10.1016/j.jmb.2007.05.022; Krokhin O, 2003, MOL CELL PROTEOMICS, V2, P346, DOI 10.1074/mcp.M300048-MCP200; Lander GC, 2009, J STRUCT BIOL, V166, P95, DOI 10.1016/j.jsb.2009.01.002; Li F, 2005, SCIENCE, V309, P1864, DOI 10.1126/science.1116480; Li WD, 2005, SCIENCE, V310, P676, DOI 10.1126/science.1118391; Li WH, 2003, NATURE, V426, P450, DOI 10.1038/nature02145; Li WH, 2005, EMBO J, V24, P1634, DOI 10.1038/sj.emboj.7600640; Liu HQ, 2015, J STRUCT BIOL, V192, P14, DOI 10.1016/j.jsb.2015.09.001; Lu GW, 2013, NATURE, V500, P227, DOI 10.1038/nature12328; MacLean B, 2010, BIOINFORMATICS, V26, P966, DOI 10.1093/bioinformatics/btq054; Matsuyama S, 2009, J VIROL, V83, P11133, DOI 10.1128/JVI.00959-09; McCoy AJ, 2018, ACTA CRYSTALLOGR D, V74, P279, DOI 10.1107/S2059798318001353; McCoy LE, 2016, CELL REP, V16, P2327, DOI 10.1016/j.celrep.2016.07.074; Memish ZA, 2013, EMERG INFECT DIS, V19, P1819, DOI 10.3201/eid1911.131172; Menachery VD, 2016, P NATL ACAD SCI USA, V113, P3048, DOI 10.1073/pnas.1517719113; Menachery VD, 2015, NAT MED, V21, P1508, DOI 10.1038/nm.3985; Michielssens S, 2014, ANGEW CHEM INT EDIT, V53, P10367, DOI 10.1002/anie.201403102; Millet J. K., 2016, BIOL PROTOC, V6; Millet JK, 2014, P NATL ACAD SCI USA, V111, P15214, DOI 10.1073/pnas.1407087111; Ng ML, 2003, J GEN VIROL, V84, P3291, DOI 10.1099/vir.0.19505-0; Nkolola JP, 2014, J VIROL, V88, P9538, DOI 10.1128/JVI.01739-14; Pallesen J, 2017, P NATL ACAD SCI USA, V114, pE7348, DOI 10.1073/pnas.1707304114; Park JE, 2016, P NATL ACAD SCI USA, V113, P12262, DOI 10.1073/pnas.1608147113; Pettersen EF, 2004, J COMPUT CHEM, V25, P1605, DOI 10.1002/jcc.20084; Punjani A, 2017, NAT METHODS, V14, P290, DOI [10.1038/nmeth.4169, 10.1038/NMETH.4169]; Raj VS, 2013, NATURE, V495, P251, DOI 10.1038/nature12005; Ritchie G, 2010, VIROLOGY, V399, P257, DOI 10.1016/j.virol.2009.12.020; Rockx B, 2008, J VIROL, V82, P3220, DOI 10.1128/JVI.02377-07; Rosenthal PB, 2003, J MOL BIOL, V333, P721, DOI 10.1016/j.jmb.2003.07.013; Rossen JWA, 1998, J VIROL, V72, P497, DOI 10.1128/JVI.72.1.497-503.1998; Scharf L, 2015, CELL, V162, P1379, DOI 10.1016/j.cell.2015.08.035; Scheres SHW, 2012, NAT METHODS, V9, P853, DOI 10.1038/nmeth.2115; Shang J., 2017, J VIROL, V92; Shang J, 2018, PLOS PATHOG, V14, DOI 10.1371/journal.ppat.1007009; Snijder J, 2018, IMMUNITY, V48, P799, DOI 10.1016/j.immuni.2018.03.026; Song WF, 2018, PLOS PATHOG, V14, DOI 10.1371/journal.ppat.1007236; Stertz S, 2007, VIROLOGY, V361, P304, DOI 10.1016/j.virol.2006.11.027; Stewart-Jones GBE, 2016, CELL, V165, P813, DOI 10.1016/j.cell.2016.04.010; Struwe WB, 2018, CELL REP, V24, P1958, DOI 10.1016/j.celrep.2018.07.080; Suloway C, 2005, J STRUCT BIOL, V151, P41, DOI 10.1016/j.jsb.2005.03.010; Traggiai E, 2004, NAT MED, V10, P871, DOI 10.1038/nm1080; Vagin AA, 2004, ACTA CRYSTALLOGR D, V60, P2184, DOI 10.1107/S0907444904023510; Veesler D, 2009, ANAL BIOCHEM, V388, P115, DOI 10.1016/j.ab.2009.02.018; Voss NR, 2009, J STRUCT BIOL, V166, P205, DOI 10.1016/j.jsb.2009.01.004; Walls AC, 2017, P NATL ACAD SCI USA, V114, P11157, DOI 10.1073/pnas.1708727114; Walls AC, 2016, NAT STRUCT MOL BIOL, V23, P899, DOI 10.1038/nsmb.3293; Walls AC, 2016, NATURE, V531, P114, DOI 10.1038/nature16988; Wang M, 2005, EMERG INFECT DIS, V11, P1860, DOI 10.3201/eid1112.041293; Wang QH, 2014, CELL HOST MICROBE, V16, P328, DOI 10.1016/j.chom.2014.08.009; Wang RYR, 2016, ELIFE, V5, DOI 10.7554/eLife.17219; Watanabe Y, 2018, P NATL ACAD SCI USA, V115, P7320, DOI 10.1073/pnas.1803990115; Yang Y, 2015, J VIROL, V89, P9119, DOI 10.1128/JVI.01279-15; Yuan Y, 2017, NAT COMMUN, V8, DOI 10.1038/ncomms15092; Zhang H, 2004, J VIROL, V78, P6938, DOI 10.1128/JVI.78.13.6938-6945.2004; Zheng SQ, 2017, NAT METHODS, V14, P331, DOI 10.1038/nmeth.4193; Zivanov J, 2019, IUCRJ, V6, P5, DOI 10.1107/S205225251801463X; Zivanov J, 2018, ELIFE, V7, DOI 10.7554/eLife.42166	84	15	15	32	46	CELL PRESS	CAMBRIDGE	50 HAMPSHIRE ST, FLOOR 5, CAMBRIDGE, MA 02139 USA	0092-8674	1097-4172		CELL	Cell	FEB 21	2019	176	5					1026	+		10.1016/j.cell.2018.12.028			29	Biochemistry & Molecular Biology; Cell Biology	Biochemistry & Molecular Biology; Cell Biology	HM2AK	WOS:000459257500008	30712865	Bronze, Green Accepted, Green Published			2020-04-01	
J	Goo, J; Jeong, Y; Park, YS; Yang, E; Jung, DI; Rho, S; Park, U; Sung, H; Park, PG; Choi, JA; Seo, SH; Cho, NH; Lee, H; Lee, JM; Kim, JO; Song, M				Goo, Junghyun; Jeong, Yuji; Park, Young-Shin; Yang, Eunji; Jung, Dae-Im; Rho, Semi; Park, Uni; Sung, Hyeyeong; Park, Pil-Gu; Choi, Jung-ah; Seo, Sang Hwan; Cho, Nam Hyuck; Lee, Hyeja; Lee, Jae Myun; Kim, Jae-Ouk; Song, Manki			Characterization of novel monoclonal antibodies against MERS-coronavirus spike protein	VIRUS RESEARCH			English	Article						MERS-CoV; Monoclonal antibody; Neutralizing antibody; Pseudovirus; Neutralization; Epitope	EAST RESPIRATORY SYNDROME; RECEPTOR-BINDING DOMAIN; SOUTH-KOREA; SINGLE PATIENT; COV; NEUTRALIZATION; IMMUNOGENICITY; TRANSMISSION; VACCINE	Middle East Respiratory Syndrome coronavirus (MERS-CoV) causes severe pulmonary infection, with similar to 35 % mortality. Spike glycoprotein (S) of MERS-CoV is a key target for vaccines and therapeutics because S mediates viral entry and membrane-fusion to host cells. Here, four different S subunit proteins, receptor-binding domain (RBD; 358-606 aa), S1 (1-751 aa), S2 (752-1296 aa), and S Delta TM (1-1296 aa), were generated using the baculoviral system and immunized in mice to develop neutralizing antibodies. We developed 77 hybridomas and selected five neutralizing mAbs by immunization with S Delta TM against MERS-CoV EMC/2012 strain S-pseudotyped lentivirus. However, all five monoclonal antibodies (mAb) did not neutralize the pseudotyped V534A mutation. Additionally, one mAb RBD-14F8 did not show neutralizing activity against pseudoviruses with amino acid substitution of L506 F or D509 G (Englandl strain, EMC/2012 L506 F, and EMC/2012 D509 G), and RBD-43E4 mAb could not neutralize the pseudotyped 1529 T mutation, while three other neutralizing mAbs showed broad neutralizing activity. This implies that the mutation in residue 506-509, 529, and 534 of S is critical to generate neutralization escape variants of MERS-CoV. Interestingly, all five neutralizing mAbs have binding affinity to RBD, although most mAbs generated by RBD did not have neutralizing activity. Additionally, chimeric antibodies of RBD-14F8 and RBD-43E4 with human Fc and light chain showed neutralizing effect against wild type MERS-CoV KOR/KNIH/002, similar to the original mouse mAbs. Thus, our mAbs can be utilized for the identification of specific mutations of MERS-CoV.	[Goo, Junghyun; Jeong, Yuji; Park, Young-Shin; Yang, Eunji; Jung, Dae-Im; Rho, Semi; Choi, Jung-ah; Seo, Sang Hwan; Kim, Jae-Ouk; Song, Manki] Int Vaccine Inst, Sci Unit, Seoul, South Korea; [Park, Uni; Cho, Nam Hyuck] Dept Microbiol & Immunol, Seoul, South Korea; [Park, Uni; Cho, Nam Hyuck] Seoul Natl Univ, Coll Med, Dept Biomed Sci, Seoul, South Korea; [Cho, Nam Hyuck] Seoul Natl Univ, Inst Endem Dis, Med Res Ctr, Seoul, South Korea; [Cho, Nam Hyuck] Bundang Hosp, Seoul, South Korea; [Lee, Hyeja] NKMAX Co Ltd, Seongnam, South Korea; [Lee, Jae Myun] Yonsei Univ, Inst Immunol & Immunol Dis, Brain Korea PLUS Project Med Sci 21, Dept Microbiol & Immunol, Seoul, South Korea	Kim, JO; Song, M (reprint author), Int Vaccine Inst, Sci Unit, Seoul, South Korea.	jokim@ivi.int; mksong@ivi.int			Ministry of Health and Welfare, Republic of KoreaMinistry of Health & Welfare, Republic of Korea [HI15C2971]	This work was supported by a grant from the Ministry of Health and Welfare, Republic of Korea (HI15C2971).	Chen YZ, 2017, EMERG MICROBES INFEC, V6, DOI 10.1038/emi.2017.50; Cho SY, 2016, LANCET, V388, P994, DOI 10.1016/S0140-6736(16)30623-7; Dang VT, 2013, CLIN DEV IMMUNOL, DOI 10.1155/2013/716961; de Groot RJ, 2013, J VIROL, V87, P7790, DOI 10.1128/JVI.01244-13; de Wit E, 2018, ANTIVIR RES, V156, P64, DOI 10.1016/j.antiviral.2018.06.006; Drosten C, 2014, NEW ENGL J MED, V371, P828, DOI 10.1056/NEJMoa1405858; Du LY, 2013, J VIROL, V87, P9939, DOI 10.1128/JVI.01048-13; Hemida MG, 2017, TRANSBOUND EMERG DIS, V64, P344, DOI 10.1111/tbed.12401; Kim DW, 2016, EMERG INFECT DIS, V22, P100, DOI 10.3201/eid2201.151055; Kim JO, 2007, J VIROL, V81, P1444, DOI 10.1128/JVI.01790-06; Kim JO, 2015, CLIN VACCINE IMMUNOL, V22, P381, DOI 10.1128/CVI.00661-14; Kim Y, 2016, MBIO, V7, DOI 10.1128/mBio.00019-16; Li DP, 2016, J VIROL, V90, P10486, DOI 10.1128/JVI.01462-16; Min CK, 2016, SCI REP-UK, V6, DOI 10.1038/srep25359; Naldini L, 1996, P NATL ACAD SCI USA, V93, P11382, DOI 10.1073/pnas.93.21.11382; Pallesen J, 2017, P NATL ACAD SCI USA, V114, pE7348, DOI 10.1073/pnas.1707304114; Park SH, 2016, INFECT CHEMOTHER, V48, P99, DOI 10.3947/ic.2016.48.2.99; Pascal KE, 2015, P NATL ACAD SCI USA, V112, P8738, DOI 10.1073/pnas.1510830112; Rao S, 2008, PLOS ONE, V3, DOI 10.1371/journal.pone.0002432; Shere KD, 1997, MOL MICROBIOL, V25, P451, DOI 10.1046/j.1365-2958.1997.4681827.x; van Boheemen S, 2012, MBIO, V3, DOI 10.1128/mBio.00473-12; Wang L, 2018, J VIROL, V92, DOI 10.1128/JVI.01060-18; Wang LS, 2015, NAT COMMUN, V6, DOI 10.1038/ncomms8712; Wang NS, 2013, CELL RES, V23, P986, DOI 10.1038/cr.2013.92; Wang QH, 2014, CELL HOST MICROBE, V16, P328, DOI 10.1016/j.chom.2014.08.009; Yang E, 2015, J MICROBIOL BIOTECHN, V25, P280, DOI 10.4014/jmb.1410.10035; Yang JS, 2015, EMERG INFECT DIS, V21, P2084, DOI 10.3201/eid2111.151016; Ying TL, 2014, J VIROL, V88, P7796, DOI 10.1128/JVI.00912-14; Yu XJ, 2015, SCI REP-UK, V5, DOI 10.1038/srep13133; Zhang SY, 2018, CELL REP, V24, P441, DOI 10.1016/j.celrep.2018.06.041	30	1	1	45	45	ELSEVIER	AMSTERDAM	RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS	0168-1702	1872-7492		VIRUS RES	Virus Res.	MAR 12	2020	278								197863	10.1016/j.virusres.2020.197863			10	Virology	Virology	KO3DZ	WOS:000515429400011	31945421	Other Gold			2020-04-01	
J	Mowbray, H				Mowbray, Heather			Letter from China: covid-19 on the grapevine, on the internet, and in commerce	BMJ-BRITISH MEDICAL JOURNAL			English	Editorial Material											hcmowbray@gmail.com	Ribeiro, Nuno/AAH-2299-2020					0	0	0	45	45	BMJ PUBLISHING GROUP	LONDON	BRITISH MED ASSOC HOUSE, TAVISTOCK SQUARE, LONDON WC1H 9JR, ENGLAND	1756-1833			BMJ-BRIT MED J	BMJ-British Medical Journal	FEB 19	2020	368								m643	10.1136/bmj.m643			2	Medicine, General & Internal	General & Internal Medicine	KO0KS	WOS:000515235300013	32075783	Bronze			2020-04-01	
J	Kim, JH; Kang, M; Park, E; Chung, DR; Kim, J; Hwang, ES				Kim, Jin Hwa; Kang, Minhee; Park, Eunkyoung; Chung, Doo Ryeon; Kim, Jiyeon; Hwang, Eung Soo			A Simple and Multiplex Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid Detection of SARS-CoV	BIOCHIP JOURNAL			English	Article						SARS-CoV; Loop-mediated isothermal amplification; Colorimetric detection; Point-of-care test	ACUTE-RESPIRATORY-SYNDROME; SYNDROME CORONAVIRUS	The current diagnosis of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) relies on laboratory-based tests since its clinical features are nonspecific, unlike other respiratory pathogens. Therefore, the development of a rapid and simple method for on-site detection of SARS-CoV is crucial for the identification and prevention of future SARS outbreaks. In this study, a simple colorimetric and multiplex loop-mediated isothermal amplification (LAMP) assay was developed to rapid screening of severe acute respiratory syndrome-associated coronavirus (SARS-CoV). It can be visually detected based on color change and monitored in real-time with fluorescent signals. The performance of this assay, based on six primers targeting open reading frame (ORF1b) and nucleocapsid (N) genes located in different regions of the SARS-CoV, was compared with real-time RT-PCR assay using various concentrations of target genes. The detection limit of the LAMP assay was comparable to that of real-time RT-PCR assay and therefore a few target RNA to 10(4)-10(5) copies could be detected within a short period of time (20-25 min). In addition, we established a multiplex real-time LAMP assay to simultaneously detect two target regions within the SARS-CoV genome. Two target sequences were amplified by specific primers in the same reaction tube and revealed that it was able to detect down to 10(5) copies. The standard curve had a linear relationship with similar amplification efficiencies. The LAMP assay results in shorter "sample-to-answer" time than conventional PCR method. Therefore, it is suitable not only for diagnosis of clinical test, but also for surveillance of SARS virus in developing countries.	[Kim, Jin Hwa; Kang, Minhee; Park, Eunkyoung] Sungkyunkwan Univ, Biomed Engn Res Ctr, Samsung Med Ctr, Smart Healthcare Res Inst,Sch Med, Seoul, South Korea; [Kang, Minhee; Park, Eunkyoung] Sungkyunkwan Univ, Dept Med Device Management & Res, SAIHST, Seoul, South Korea; [Chung, Doo Ryeon] Samsung Med Ctr, Ctr Infect Prevent & Control, Seoul, South Korea; [Chung, Doo Ryeon] APFID, Seoul, South Korea; [Chung, Doo Ryeon] Sungkyunkwan Univ, Samsung Med Ctr, Dept Internal Med, Div Infect Dis,Sch Med, Seoul, South Korea; [Kim, Jiyeon; Hwang, Eung Soo] Seoul Natl Univ, Dept Microbiol & Immunol, Coll Med, Seoul, South Korea; [Kim, Jiyeon; Hwang, Eung Soo] Seoul Natl Univ, Inst Endem Dis, Med Res Ctr, Seoul, South Korea	Kang, M (reprint author), Sungkyunkwan Univ, Biomed Engn Res Ctr, Samsung Med Ctr, Smart Healthcare Res Inst,Sch Med, Seoul, South Korea.; Kang, M (reprint author), Sungkyunkwan Univ, Dept Med Device Management & Res, SAIHST, Seoul, South Korea.; Chung, DR (reprint author), Samsung Med Ctr, Ctr Infect Prevent & Control, Seoul, South Korea.; Chung, DR (reprint author), APFID, Seoul, South Korea.; Chung, DR (reprint author), Sungkyunkwan Univ, Samsung Med Ctr, Dept Internal Med, Div Infect Dis,Sch Med, Seoul, South Korea.	minhee.kang@samsung.com; minikang@skku.edu		Kang, Minhee/0000-0003-0330-7828	government-wide R&D Fund for the research of infectious diseases in Republic of Korea [HG18C0062]; Bio & Medical Technology Development Program of the National Research Foundation (NRF) - Ministry of Science [2016M3A9B6919187, 2016M3A9B6919189]	This research was supported by the government-wide R&D Fund for the research of infectious diseases in Republic of Korea (HG18C0062), and the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Ministry of Science (2016M3A9B6919187, 2016M3A9B6919189).	Almazan F, 2014, VIRUS RES, V189, P262, DOI 10.1016/j.virusres.2014.05.026; Chan JFW, 2015, CLIN MICROBIOL REV, V28, P465, DOI 10.1128/CMR.00102-14; Cheng VCC, 2007, CLIN MICROBIOL REV, V20, P660, DOI 10.1128/CMR.00023-07; Deng HW, 2000, BIOTECHNIQUES, V29, P298, DOI 10.2144/00292st05; Gadkar VJ, 2018, SCI REP-UK, V8, DOI 10.1038/s41598-018-23930-1; Gong Shu-Ran, 2018, Animal Model Exp Med, V1, P125, DOI 10.1002/ame2.12017; He ZP, 2007, VIROL J, V4, DOI 10.1186/1743-422X-4-32; Hill J, 2008, J CLIN MICROBIOL, V46, P2800, DOI 10.1128/JCM.00152-08; Iwamoto T, 2003, J CLIN MICROBIOL, V41, P2616, DOI 10.1128/JCM.41.6.2616-2622.2003; Kargar M, 2012, INDIAN J VIROL, V23, P18, DOI 10.1007/s13337-012-0067-2; Kenneth J, 2003, NATL MED J INDIA, V16, P115; Kong QM, 2012, PARASITE VECTOR, V5, DOI 10.1186/1756-3305-5-2; Ksiazek TG, 2003, NEW ENGL J MED, V348, P1953, DOI 10.1056/NEJMoa030781; Kubota R, 2008, PHYTOPATHOLOGY, V98, P1045, DOI 10.1094/PHYTO-98-9-1045; Kuiken T, 2003, LANCET, V362, P263, DOI 10.1016/S0140-6736(03)13967-0; Lim Y.X., 2016, DISEASES, V4, P26, DOI DOI 10.3390/DISEASES4030026; Marra MA, 2003, SCIENCE, V300, P1399, DOI 10.1126/science.1085953; Mori Y, 2001, BIOCHEM BIOPH RES CO, V289, P150, DOI 10.1006/bbrc.2001.5921; Njiru ZK, 2012, PLOS NEGLECT TROP D, V6, DOI 10.1371/journal.pntd.0001572; Notomi T, 2000, NUCLEIC ACIDS RES, V28, DOI 10.1093/nar/28.12.e63; Peiris JSM, 2003, LANCET, V361, P1319, DOI 10.1016/S0140-6736(03)13077-2; Poole CB, 2015, PLOS ONE, V10, DOI 10.1371/journal.pone.0139286; Poon LLM, 2004, CLIN CHEM, V50, P1050, DOI 10.1373/clinchem.2004.032011; Viana GMR, 2018, PLOS ONE, V13, DOI 10.1371/journal.pone.0200492; Rahman SMM, 2017, PLOS NEGLECT TROP D, V11, DOI 10.1371/journal.pntd.0005995; Schlagenhauf P, 2003, LANCET, V361, P1017, DOI 10.1016/S0140-6736(03)12843-7; Shirato K, 2018, J VIROL METHODS, V258, P41, DOI 10.1016/j.jviromet.2018.05.006; Sun MM, 2019, INT J MOL SCI, V20, DOI 10.3390/ijms20081937; Thai HTC, 2004, J CLIN MICROBIOL, V42, P1956, DOI 10.1128/JCM.42.5.1956-1961.2004; Tomlinson JA, 2010, LETT APPL MICROBIOL, V51, P650, DOI 10.1111/j.1472-765X.2010.02949.x; Tsang KW, 2003, NEW ENGL J MED, V348, P1977, DOI 10.1056/NEJMoa030666; Wang YZ, 2017, INT J NANOMED, V12, P4789, DOI 10.2147/IJN.S137338; Wong SCC, 2005, J CLIN PATHOL, V58, P276, DOI 10.1136/jcp.2004.016592; Wu HS, 2004, EMERG INFECT DIS, V10, P304; Yoneyama T, 2007, J VIROL METHODS, V145, P162, DOI 10.1016/j.jviromet.2007.05.023; Zhang X, 2013, PLOS ONE, V8, DOI 10.1371/journal.pone.0082841; Zhou QJ, 2014, J MICROBIOL METH, V104, P26, DOI 10.1016/j.mimet.2014.06.008	37	0	0	45	45	KOREAN BIOCHIP SOCIETY-KBCS	SEOUL	KOREA SCI & TECHNOL CENT, #310, 635-4, YEOGSAM-DONG, KANGNAM-GU, SEOUL, 135-703, SOUTH KOREA	1976-0280	2092-7843		BIOCHIP J	BioChip J.	DEC	2019	13	4					341	351		10.1007/s13206-019-3404-3		NOV 2019	11	Biochemical Research Methods; Chemistry, Analytical; Nanoscience & Nanotechnology	Biochemistry & Molecular Biology; Chemistry; Science & Technology - Other Topics	KJ0DA	WOS:000496262000003		Bronze			2020-04-01	
J	Liu, P; Tan, XZ				Liu, Peng; Tan, Xian-zheng			2019 Novel Coronavirus (2019-nCoV) Pneumonia	RADIOLOGY			English	Editorial Material									[Liu, Peng; Tan, Xian-zheng] Hunan Normal Univ, Affiliated Hosp 1, Dept Radiol, Hunan Prov Peoples Hosp, Changsha 410005, Peoples R China	Tan, XZ (reprint author), Hunan Normal Univ, Affiliated Hosp 1, Dept Radiol, Hunan Prov Peoples Hosp, Changsha 410005, Peoples R China.	xianzhengtan@163.com		tan, xianzheng/0000-0001-9319-1237			Chung M, RADIOLOGY; Huang C, 2020, LANCET; Kanne J., RADIOLOGY; Lei J, 2020, RADIOLOGY; Zhu N, 2020, N ENGL J MED	5	1	1	44	44	RADIOLOGICAL SOC NORTH AMERICA	OAK BROOK	820 JORIE BLVD, OAK BROOK, IL 60523 USA	0033-8419			RADIOLOGY	Radiology	APR	2020	295	1					19	19		10.1148/radiol.2020200257			1	Radiology, Nuclear Medicine & Medical Imaging	Radiology, Nuclear Medicine & Medical Imaging	KV1EC	WOS:000520166000005	32013795	Bronze			2020-04-01	
J	Ruan, QR; Yang, K; Wang, WX; Jiang, LY; Song, JX				Ruan, Qiurong; Yang, Kun; Wang, Wenxia; Jiang, Lingyu; Song, Jianxin			Clinical predictors of mortality due to COVID-19 based on an analysis of data of 150 patients from Wuhan, China	INTENSIVE CARE MEDICINE			English	Letter; Early Access									[Ruan, Qiurong] Huazhong Univ Sci & Technol, Inst Pathol, Tongji Hosp, Tongji Med Coll, 1095 Jiefang Ave, Wuhan 430030, Hubei, Peoples R China; [Ruan, Qiurong] Huazhong Univ Sci & Technol, Sch Basic Med, Dept Pathol, Tongji Med Coll, 1095 Jiefang Ave, Wuhan 430030, Hubei, Peoples R China; [Yang, Kun] Huazhong Univ Sci & Technol, Dept Dermatol, Tongji Med Coll, Tongji Hosp, 1095 Jiefang Ave, Wuhan 430030, Hubei, Peoples R China; [Wang, Wenxia; Song, Jianxin] Huazhong Univ Sci & Technol, Tongji Hosp, Dept Infect Dis, Tongji Med Coll, 1095 Jiefang Ave, Wuhan 430030, Hubei, Peoples R China; [Jiang, Lingyu] Huazhong Univ Sci & Technol, Tongji Hosp, Dept Clin Immunol, Tongji Med Coll, 1095 Jiefang Ave, Wuhan 430030, Hubei, Peoples R China	Song, JX (reprint author), Huazhong Univ Sci & Technol, Tongji Hosp, Dept Infect Dis, Tongji Med Coll, 1095 Jiefang Ave, Wuhan 430030, Hubei, Peoples R China.	songsingsjx@sina.com	; Yang, Kun/J-7229-2013	song, jianxin/0000-0002-4090-6670; Yang, Kun/0000-0001-6729-2552	Emergency Project for the Prevention and Control of the Novel Coronavirus (SARS-CoV-2) Outbreak in Hubei Province, China; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81703174, 81602722]	This work was supported by the Emergency Project for the Prevention and Control of the Novel Coronavirus (SARS-CoV-2) Outbreak in Hubei Province, China, as well as the National Natural Science Foundation of China (81703174 and 81602722). The funding source had no role in the design and conduct of the study, the analysis and interpretation of the data, or in the writing of the manuscript.	Chen NS, 2020, LANCET, V395, P507, DOI 10.1016/S0140-6736(20)30211-7; Mahase E, 2020, BMJ-BRIT MED J, V368, DOI 10.1136/bmj.m641; Wang DW, 2019, SCI CHINA LIFE SCI, V62, P187, DOI 10.1007/s11427-018-9385-3	3	1	1	44	44	SPRINGER	NEW YORK	ONE NEW YORK PLAZA, SUITE 4600, NEW YORK, NY, UNITED STATES	0342-4642	1432-1238		INTENS CARE MED	Intensive Care Med.												10.1007/s00134-020-05991-x		MAR 2020	3	Critical Care Medicine	General & Internal Medicine	KS1EV	WOS:000518054400003	32125452	Bronze			2020-04-01	
J	Shang, WL; Yang, Y; Rao, YF; Rao, XC				Shang, Weilong; Yang, Yi; Rao, Yifan; Rao, Xiancai			The outbreak of SARS-CoV-2 pneumonia calls for viral vaccines	NPJ VACCINES			English	Article							SPIKE PROTEIN; SARS-COV; NEUTRALIZATION; IMMUNIZATION	The outbreak of 2019-novel coronavirus disease (COVID-19) that is caused by SARS-CoV-2 has spread rapidly in China, and has developed to be a Public Health Emergency of International Concern. However, no specific antiviral treatments or vaccines are available yet. This work aims to share strategies and candidate antigens to develop safe and effective vaccines against SARS-CoV-2.	[Shang, Weilong; Yang, Yi; Rao, Yifan; Rao, Xiancai] Third Mil Med Univ, Army Med Univ, Coll Basic Med Sci, Dept Microbiol, Chongqing 400038, Peoples R China	Rao, XC (reprint author), Third Mil Med Univ, Army Med Univ, Coll Basic Med Sci, Dept Microbiol, Chongqing 400038, Peoples R China.	raoxiancai@126.com			National Key Biosafety Technology Research and Development Program of China [2017YFC1200404-4]; Biosafety Research Program of PLA [17SAZ08]	This work was supported by the National Key Biosafety Technology Research and Development Program of China (2017YFC1200404-4), and the Biosafety Research Program of PLA (17SAZ08).	Bukreyev A, 2004, LANCET, V363, P2122, DOI 10.1016/S0140-6736(04)16501-X; Chan JFW, 2020, EMERG MICROBES INFEC, V9, P221, DOI 10.1080/22221751.2020.1719902; Du LY, 2009, NAT REV MICROBIOL, V7, P226, DOI 10.1038/nrmicro2090; Duan YN, 2020, RADIOLOGY, V295, P21, DOI 10.1148/radiol.2020200323; Eyigun CP, 1998, J VIRAL HEPATITIS, V5, P265, DOI 10.1046/j.1365-2893.1998.00106.x; Graham RL, 2013, NAT REV MICROBIOL, V11, P836, DOI 10.1038/nrmicro3143; Gralinski LE, 2020, VIRUSES-BASEL, V12, DOI 10.3390/v12020135; Grohskopf LA, 2018, MMWR RECOMM REP, V67, DOI 10.15585/mmwr.rr6703a1; Guan W, 2020, CLIN CHARACTERISTICS, DOI [10.1101/2020.02.06.20020974, DOI 10.1101/2020.02.06.20020974]; He WQ, 2015, METHODS, V90, P95, DOI 10.1016/j.ymeth.2015.04.037; Holshue ML, 2020, NEW ENGL J MED, V382, P929, DOI 10.1056/NEJMoa2001191; Huang CL, 2020, LANCET, V395, P497, DOI 10.1016/S0140-6736(20)30183-5; Jiang SB, 2005, EMERG INFECT DIS, V11, P1016, DOI 10.3201/1107.050219; Li Qun, 2020, N Engl J Med, V382, P1199, DOI 10.1056/NEJMoa2001316; National Health Commission of the People's Republic of China, EP PREV CONTR DYN; Rauch S, 2018, FRONT IMMUNOL, V9, DOI 10.3389/fimmu.2018.01963; Regla-Nava JA, 2015, J VIROL, V89, P3870, DOI 10.1128/JVI.03566-14; Roper RL, 2009, EXPERT REV VACCINES, V8, P887, DOI [10.1586/erv.09.43, 10.1586/ERV.09.43]; Schoeman D, 2019, VIROL J, V16, DOI 10.1186/s12985-019-1182-0; Shi SQ, 2006, MOL IMMUNOL, V43, P1791, DOI 10.1016/j.molimm.2005.11.005; Song ZQ, 2019, VIRUSES-BASEL, V11, DOI 10.3390/v11010059; Wan Yushun, 2020, J Virol, V94, DOI 10.1128/JVI.00127-20; Wu F, 2020, NATURE, DOI 10.1038/s41586-020-2008-3; Yang ZY, 2004, NATURE, V428, P561, DOI 10.1038/nature02463; Yuan JZ, 2018, NANO LETT, V18, P725, DOI 10.1021/acs.nanolett.7b03893	25	0	0	43	43	NATURE PUBLISHING GROUP	LONDON	MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND		2059-0105		NPJ VACCINES	npj Vaccines	MAR 6	2020	5	1							18	10.1038/s41541-020-0170-0			3	Immunology; Medicine, Research & Experimental	Immunology; Research & Experimental Medicine	KS5SB	WOS:000518367000001	32194995	DOAJ Gold, Green Published			2020-04-01	
J	Giovanetti, M; Benvenuto, D; Angeletti, S; Ciccozzi, M				Giovanetti, Marta; Benvenuto, Domenico; Angeletti, Silvia; Ciccozzi, Massimo			The first two cases of 2019-nCoV in Italy: Where they come from?	JOURNAL OF MEDICAL VIROLOGY			English	Article						epidemiology; engineering and technology; infection; macromolecular design		A novel Coronavirus, 2019-nCoV, has been identified as the causal pathogen of an ongoing epidemic, with the first cases reported in Wuhan, China, last December 2019, and has since spread to other countries worldwide, included Europe and very recently Italy. In this short report, phylogenetic reconstruction was used to better understand the transmission dynamics of the virus from its first introduction in China focusing on the more recent evidence of infection in a couple of Chinese tourists arrived in Italy on 23rd January 2020 and labeled as Coronavirus Italian cases. A maximum clade credibility tree has been built using a dataset of 54 genome sequences of 2019-nCoV plus two closely related bat strains (SARS-like CoV) available in GenBank. Bayesian time-scaled phylogenetic analysis was implemented in BEAST 1.10.4. The Bayesian phylogenetic reconstruction showed that 2019-2020 nCoV firstly introduced in Wuhan on 25 November 2019, started epidemic transmission reaching many countries worldwide, including Europe and Italy where the two strains isolated dated back 19 January 2020, the same that the Chinese tourists arrived in Italy. Strains isolated outside China were intermixed with strains isolated in China as evidence of likely imported cases in Rome, Italy, and Europe, as well. In conclusion, this report suggests that further spread of 2019-nCoV epidemic was supported by human mobility and that quarantine of suspected or diagnosed cases is useful to prevent further transmission. Viral genome phylogenetic analysis represents a useful tool for the evaluation of transmission dynamics and preventive action.	[Giovanetti, Marta] Fundacao Oswaldo Cruz, Inst Oswaldo Cruz, Lab Flavivirus, Rio De Janeiro, Brazil; [Benvenuto, Domenico; Ciccozzi, Massimo] Univ Campus Biomed Rome, Unit Med Stat & Mol Epidemiol, Rome, Italy; [Angeletti, Silvia] Univ Campus Biomed Rome, Unit Clin Lab Sci, I-00128 Rome, Italy	Angeletti, S (reprint author), Univ Campus Biomed Rome, Unit Clin Lab Sci, I-00128 Rome, Italy.	s.angeletti@unicampus.it	Ribeiro, Nuno/AAH-2299-2020; ciccozzi, massimo/C-6484-2016	ciccozzi, massimo/0000-0003-3866-9239; Benvenuto, Domenico/0000-0003-3833-2927			[Anonymous], 2020, LANCET, V395, P311, DOI 10.1016/S0140-6736(20)30186-0; Baele G, 2013, MOL BIOL EVOL, V30, P239, DOI 10.1093/molbev/mss243; Benvenuto D, 2020, J MED VIROL, V92, P455, DOI 10.1002/jmv.25688; Darriba D, 2012, NAT METHODS, V9, P772, DOI 10.1038/nmeth.2109; Drummond AJ, 2006, PLOS BIOL, V4, P699, DOI 10.1371/journal.pbio.0040088; Hall T.A., 1999, NUCL ACIDS S SER, V41, P95, DOI DOI 10.1021/BK-1999-0734.CH008; Huang CL, 2020, LANCET, V395, P497, DOI 10.1016/S0140-6736(20)30183-5; Hui DS, 2020, INT J INFECT DIS, V91, P264, DOI 10.1016/j.ijid.2020.01.009; Katoh K, 2019, BRIEF BIOINFORM, V20, P1160, DOI 10.1093/bib/bbx108; Rambaut A, 2018, SYST BIOL, V67, P901, DOI 10.1093/sysbio/syy032; Rambaut A, 2016, VIRUS EVOL, V2, DOI 10.1093/ve/vew007; Suchard MA, 2018, VIRUS EVOL, V4, DOI 10.1093/ve/vey016; Zhu N, 2020, NEW ENGL J MED, V382, P727, DOI 10.1056/NEJMoa2001017	13	0	0	43	43	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0146-6615	1096-9071		J MED VIROL	J. Med. Virol.	MAY	2020	92	5					518	521		10.1002/jmv.25699		FEB 2020	4	Virology	Virology	KU3AY	WOS:000512724700001	32022275	Bronze			2020-04-01	
J	More, GD; Dunowska, M; Acke, E; Cave, NJ				More, G. D.; Dunowska, M.; Acke, E.; Cave, N. J.			A serological survey of canine respiratory coronavirus in New Zealand	NEW ZEALAND VETERINARY JOURNAL			English	Article						Canine respiratory coronavirus; ELISA; seroprevalence; New Zealand; survey	GROUP-2 CORONAVIRUS; INFLUENZA-VIRUS; PREVALENCE; DOGS; DISEASE	Aims: To determine the seroprevalence of canine respiratory coronavirus (CRCoV) in New Zealand dogs, and to explore associations with age, sex, breed, month, and geographical region of sampling and reported presence of clinical signs suggestive of respiratory disease. Methods: A total of 1,015 canine serum samples were randomly selected from submissions to a diagnostic laboratory between March and December 2014, and were analysed for CRCoV antibodies using a competitive ELISA. Logistic regression analysis was used to determine associations between seroprevalence of CRCoV and breed category, age, sex, sampling month, region, and reported health status of dogs. Results: Overall, 538/1,015 (53.0%) samples were seropositive for CRCoV, with 492/921 (53.4%) positive dogs in the North Island and 46/94 (49%) in the South Island. Age of dog, sampling month, region, and presence of abnormal respiratory signs were included in the initial logistic regression model. Seroprevalence was higher in dogs aged >= 3 compared with <= 2 years (p<0.01). The lowest seroprevalence was observed in July (30/105; 28.5%) and August (32/100; 32%), and the highest in June (74/100; 74%). Seroprevalence in dogs from Auckland was higher than in dogs from the Hawkes Bay, Manawatu, Marlborough, and Waikato regions (p<0.05). Abnormal respiratory signs (coughing, nasal discharge, or sneezing) were reported for 28/1,015 (2.8%) dogs sampled. Seroprevalence for CRCoV tended to be higher among dogs with respiratory signs (67.9 (95% CI=47.6-83.4)%) than dogs with no reported respiratory signs (52.6 (95% CI=49.5-55.7)%). Conclusions: Serological evidence of infection with CRCoV was present in more than half of the dogs tested from throughout New Zealand. Differences in CRCoV seroprevalence between regions and lack of seasonal pattern indicate that factors other than external temperatures may be important in the epidemiology of CRCoV in New Zealand.	[More, G. D.; Dunowska, M.; Acke, E.; Cave, N. J.] Massey Univ, Sch Vet Sci, Palmerston North, New Zealand; [Acke, E.] Vet Med Lab GmbH, IDEXX Labs, Ludwigsburg, Germany	Dunowska, M (reprint author), Massey Univ, Sch Vet Sci, Palmerston North, New Zealand.	m.dunowska@massey.ac.nz			New Zealand Greyhound Racing Association	Sincere thanks to Raewynne Pearson and Adrienne French for supplying the serum samples for ELISA and to Janis Bridges for help with statistical analysis. This study was partly funded by the New Zealand Greyhound Racing Association.	An DJ, 2010, J VET MED SCI, V72, P1217, DOI 10.1292/jvms.10-0067; Decaro N, 2007, VET MICROBIOL, V121, P225, DOI 10.1016/j.vetmic.2006.12.001; Ellis JA, 2005, CAN VET J, V46, P447; Erles K, 2005, ARCH VIROL, V150, P1493, DOI 10.1007/s00705-005-0533-x; Erles K, 2003, VIROLOGY, V310, P216, DOI 10.1016/S0042-6822(03)00160-0; Eyles K, 2008, VET CLIN N AM-SMALL, V38, P815, DOI 10.1016/j.cvsm.2008.02.008; Kaneshima T, 2006, J VET MED SCI, V68, P21, DOI 10.1292/jvms.68.21; Knesl O, 2009, NEW ZEAL VET J, V57, P295, DOI 10.1080/00480169.2009.58624; Mitchell JA, 2017, VET MICROBIOL, V212, P31, DOI 10.1016/j.vetmic.2017.10.019; Priestnall SL, 2007, J VET DIAGN INVEST, V19, P176, DOI 10.1177/104063870701900206; Priestnall SL, 2006, VET MICROBIOL, V115, P43, DOI 10.1016/j.vetmic.2006.02.008; Soma T, 2008, VET REC, V163, P368, DOI 10.1136/vr.163.12.368; Sowman HR, 2018, NEW ZEAL VET J, V66, P236, DOI 10.1080/00480169.2018.1490214	13	0	0	42	43	TAYLOR & FRANCIS LTD	ABINGDON	2-4 PARK SQUARE, MILTON PARK, ABINGDON OR14 4RN, OXON, ENGLAND	0048-0169	1176-0710		NEW ZEAL VET J	N. Z. Vet. J.	JAN 2	2020	68	1					54	59		10.1080/00480169.2019.1667282			6	Veterinary Sciences	Veterinary Sciences	JS8RS	WOS:000500569700001	31513753	Bronze			2020-04-01	
J	Zhan, SY; Yang, YY; Fu, CX				Zhan, Siyi; Yang, Ying Ying; Fu, Chuanxi			Public's early response to the novel coronavirus-infected pneumonia	EMERGING MICROBES & INFECTIONS			English	Letter									[Zhan, Siyi; Yang, Ying Ying; Fu, Chuanxi] Zhejiang Chinese Med Univ, Sch Publ Hlth, 548 Binwen Rd, Hangzhou 310053, Peoples R China	Fu, CX (reprint author), Zhejiang Chinese Med Univ, Sch Publ Hlth, 548 Binwen Rd, Hangzhou 310053, Peoples R China.	fuchuanxi@gmail.com					Li Qun, 2020, N Engl J Med, V382, P1199, DOI 10.1056/NEJMoa2001316; MacIntyre CR, 2017, INFLUENZA OTHER RESP, V11, P511, DOI 10.1111/irv.12474; National Health Committee of the People's Republic of China, UPD PNEUM 2019 NCOV; Wong TW, 2005, AM J INFECT CONTROL, V33, P580, DOI 10.1016/j.ajic.2005.05.025; World Health Organization, 14 WHO	5	0	0	43	43	TAYLOR & FRANCIS LTD	ABINGDON	2-4 PARK SQUARE, MILTON PARK, ABINGDON OR14 4RN, OXON, ENGLAND		2222-1751		EMERG MICROBES INFEC	Emerg. Microbes Infect.	JAN 1	2020	9	1					534	534		10.1080/22221751.2020.1732232			1	Immunology; Microbiology	Immunology; Microbiology	KS9ZO	WOS:000518670100001	32122250	DOAJ Gold, Green Accepted			2020-04-01	
J	Chiolero, A				Chiolero, Arnaud			CORONAVIRUS COVERAGE Covid-19: a digital epidemic	BMJ-BRITISH MEDICAL JOURNAL			English	Letter									[Chiolero, Arnaud] Univ Fribourg, PopHealthLab, Populat Hlth Lab, CH-1700 Fribourg, Switzerland	Chiolero, A (reprint author), Univ Fribourg, PopHealthLab, Populat Hlth Lab, CH-1700 Fribourg, Switzerland.	achiolero@gmail.com					Candeais V, 2018, HUMAN COSTS EPIDEMIC; Gigerenzer G, 2015, RISK SAVVY MAKE GOOD; MOWBRAY H, 2020, BMJ-BRIT MED J, V368, DOI DOI 10.1136/BMJ.M516	3	0	0	42	42	BMJ PUBLISHING GROUP	LONDON	BRITISH MED ASSOC HOUSE, TAVISTOCK SQUARE, LONDON WC1H 9JR, ENGLAND	1756-1833			BMJ-BRIT MED J	BMJ-British Medical Journal	MAR 2	2020	368								m764	10.1136/bmj.m764			1	Medicine, General & Internal	General & Internal Medicine	KU2KC	WOS:000519536400004	32122876	Bronze			2020-04-01	
J	Gao, PC; Zhang, H; Wu, ZW; Wang, JC				Gao, Peichao; Zhang, Hong; Wu, Zhiwei; Wang, Jicheng			Visualising the expansion and spread of coronavirus disease 2019 by cartograms	ENVIRONMENT AND PLANNING A-ECONOMY AND SPACE			English	Article; Early Access						Cartogram; visualisation; graphic		Coronavirus disease 2019 (COVID-19) has emerged as a growing focus of global attention and a critical factor in public-health decision making. Towards fighting the COVID-19 outbreak, countries worldwide and international organisations have taken various actions, including promoting the transparency of and public access to disease data. In such public communications, maps have played an important role in that a map is worth a thousand words. Most of these have taken the form of a choropleth map. Here, we propose employing cartograms to visualise both the expansion and spread of COVID-19. We designed a combination of six circular cartograms containing the data of confirmed cases every 48 hours from 24 January to 3 February 2020. Such a design conveys both spatial and temporal information more intuitively and efficiently, so it can be expected to facilitate better public participation in the fight against COVID-19.	[Gao, Peichao] Beijing Normal Univ, State Key Lab Earth Surface Proc & Resource Ecol, Beijing, Peoples R China; [Gao, Peichao] Beijing Normal Univ, Fac Geog Sci, Beijing, Peoples R China; [Zhang, Hong; Wu, Zhiwei; Wang, Jicheng] Southwest Jiaotong Univ, Fac Geosci & Environm Engn, Chengdu 611756, Sichuan, Peoples R China; [Wang, Jicheng] Hong Kong Polytech Univ, Geoinformat, Hong Kong, Peoples R China; [Wang, Jicheng] Dept Land Surveying, Chengdu, Sichuan, Peoples R China	Zhang, H (reprint author), Southwest Jiaotong Univ, Fac Geosci & Environm Engn, Chengdu 611756, Sichuan, Peoples R China.	zhangh2011@swjtu.edu.cn			Fundamental Research Funds for the Central UniversitiesFundamental Research Funds for the Central Universities [2019NTST02]	The author(s) disclosed receipt of the following financial support for the research, authorship and/or publication of this article: This research was supported by the Fundamental Research Funds for the Central Universities (Grant No. 2019NTST02).	[Anonymous], 2020, NOV COR 2019 NCOV; Dorling D, 1996, AREA CARTOGRAMS THEI; Gao PC, 2019, J SPAT SCI, V64, P239, DOI 10.1080/14498596.2018.1440649; Harrower M, 2003, CARTOGR J, V40, P27, DOI 10.1179/000870403235002042	4	0	0	40	40	SAGE PUBLICATIONS INC	THOUSAND OAKS	2455 TELLER RD, THOUSAND OAKS, CA 91320 USA	0308-518X	1472-3409		ENVIRON PLANN A	Environ. Plan. A											0308518X20910162	10.1177/0308518X20910162		FEB 2020	4	Environmental Studies; Geography	Environmental Sciences & Ecology; Geography	KQ4LZ	WOS:000516897400001		Bronze			2020-04-01	
J	Chen, WL; Lan, Y; Yuan, XZ; Deng, XL; Li, YP; Cai, XL; Li, LY; He, RY; Tan, YZ; Deng, XZ; Gao, M; Tang, GF; Zhao, LZ; Wang, JL; Fan, QH; Wen, CY; Tong, YW; Tang, YB; Hu, FY; Li, F; Tang, XP				Chen, Weilie; Lan, Yun; Yuan, Xiaozhen; Deng, Xilong; Li, Yueping; Cai, Xiaoli; Li, Liya; He, Ruiying; Tan, Yizhou; Deng, Xizi; Gao, Ming; Tang, Guofang; Zhao, Lingzhai; Wang, Jinlin; Fan, Qinghong; Wen, Chunyan; Tong, Yuwei; Tang, Yangbo; Hu, Fengyu; Li, Feng; Tang, Xiaoping			Detectable 2019-nCoV viral RNA in blood is a strong indicator for the further clinical severity	EMERGING MICROBES & INFECTIONS			English	Article						2019-ncov; laboratory test; blood viral RNA; pharyngeal swab; anal swab	CORONAVIRUS; PNEUMONIA; WUHAN	The novel coronavirus (2019-nCoV) infection caused pneumonia. we retrospectively analyzed the virus presence in the pharyngeal swab, blood, and the anal swab detected by real-time PCR in the clinical lab. Unexpectedly, the 2109-nCoV RNA was readily detected in the blood (6 of 57 patients) and the anal swabs (11 of 28 patients). Importantly, all of the 6 patients with detectable viral RNA in the blood cohort progressed to severe symptom stage, indicating a strong correlation of serum viral RNA with the disease severity (p-value = 0.0001). Meanwhile, 8 of the 11 patients with annal swab virus-positive was in severe clinical stage. However, the concentration of viral RNA in the anal swab (Ct value = 24 + 39) was higher than in the blood (Ct value = 34 + 39) from patient 2, suggesting that the virus might replicate in the digestive tract. Altogether, our results confirmed the presence of virus RNA in extra-pulmonary sites.	[Chen, Weilie; Lan, Yun; Yuan, Xiaozhen; Deng, Xilong; Li, Yueping; Cai, Xiaoli; Li, Liya; He, Ruiying; Tan, Yizhou; Deng, Xizi; Gao, Ming; Tang, Guofang; Zhao, Lingzhai; Wang, Jinlin; Fan, Qinghong; Wen, Chunyan; Tong, Yuwei; Tang, Yangbo; Hu, Fengyu; Li, Feng; Tang, Xiaoping] Guangzhou Med Univ, Guangzhou Peoples Hosp 8, 627 Dongfeng Dong Rd, Guangzhou 510060, Guangdong, Peoples R China	Hu, FY; Li, F; Tang, XP (reprint author), Guangzhou Med Univ, Guangzhou Peoples Hosp 8, 627 Dongfeng Dong Rd, Guangzhou 510060, Guangdong, Peoples R China.	gz8h_lifeng@126.com; gz8hhfy@126.com; tangxiaopinggz@163.com			National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81670536, 81770593]; National Science and Technology Major Project [2017ZX10202203-004-002]	We declare no competing interest. We thank all the physicians and nurses who cared these patients. This work was supported by National Natural Science Foundation of China Grant (No. 81670536 and 81770593) and by the National Science and Technology Major Project (2017ZX10202203-004-002).	Chen L, 2020, Zhonghua Jie He He Hu Xi Za Zhi, V43, pE005, DOI 10.3760/cma.j.issn.1001-0939.2020.0005; Chen LJ, 2020, EMERG MICROBES INFEC, V9, P313, DOI 10.1080/22221751.2020.1725399; Chen NS, 2020, LANCET, V395, P507, DOI 10.1016/S0140-6736(20)30211-7; Hamming I, 2004, J PATHOL, V203, P631, DOI 10.1002/path.1570; Holshue M.L, 2020, NEW ENGL J MED, DOI [10.1056/NEJMoa2001191, DOI 10.1056/NEJM0A2001191]; Huang CL, 2020, LANCET, V395, P497, DOI 10.1016/S0140-6736(20)30183-5; Wan Yushun, 2020, J Virol, V94, DOI 10.1128/JVI.00127-20; Wu F, 2020, NATURE, DOI 10.1038/s41586-020-2008-3; Zhou P, 2020, NATURE, DOI 10.1038/s41586-020-2012-7; Zhou P, 2018, NATURE, V556, P255, DOI 10.1038/s41586-018-0010-9	10	0	0	40	40	TAYLOR & FRANCIS LTD	ABINGDON	2-4 PARK SQUARE, MILTON PARK, ABINGDON OR14 4RN, OXON, ENGLAND		2222-1751		EMERG MICROBES INFEC	Emerg. Microbes Infect.	JAN 1	2020	9	1					469	473		10.1080/22221751.2020.1732837			5	Immunology; Microbiology	Immunology; Microbiology	KR3MS	WOS:000517523100001	32102625	DOAJ Gold, Green Published			2020-04-01	
J	Jo, S; Kim, S; Shin, DH; Kim, MS				Jo, Seri; Kim, Suwon; Shin, Dong Hae; Kim, Mi-Sun			Inhibition of SARS-CoV 3CL protease by flavonoids	JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY			English	Article						SARS-CoV; SARS-CoV 3CLpro; flavonoid; FRET; inhibitory compounds	RESPIRATORY-SYNDROME; 3C-LIKE PROTEASE; CORONAVIRUS; DERIVATIVES; INSIGHTS; DRUGS	There were severe panics caused by Severe Acute Respiratory Syndrome (SARS) and Middle-East Respiratory Syndrome-Coronavirus. Therefore, researches targeting these viruses have been required. Coronaviruses (CoVs) have been rising targets of some flavonoids. The antiviral activity of some flavonoids against CoVs is presumed directly caused by inhibiting 3C-like protease (3CLpro). Here, we applied a flavonoid library to systematically probe inhibitory compounds against SARS-CoV 3CLpro. Herbacetin, rhoifolin and pectolinarin were found to efficiently block the enzymatic activity of SARS-CoV 3CLpro. The interaction of the three flavonoids was confirmed using a tryptophan-based fluorescence method, too. An induced-fit docking analysis indicated that S1, S2 and S3 ' sites are involved in binding with flavonoids. The comparison with previous studies showed that Triton X-100 played a critical role in objecting false positive or overestimated inhibitory activity of flavonoids. With the systematic analysis, the three flavonoids are suggested to be templates to design functionally improved inhibitors.	Ewha W Univ, Coll Pharm, Seoul, South Korea; Ewha W Univ, Grad Sch Pharmaceut Sci, Seoul, South Korea	Shin, DH; Kim, MS (reprint author), Ewha W Univ, Dept Pharm, Seoul 03760, South Korea.	dhshin55@ewha.ac.kr; shfwk31@ewha.ac.kr			National Research Foundation of Korea - Ministry of Education, Science and Technology, Republic of Korea (MEST) [2018R1D1A1B07050781, 2018R1 D1A1B07050942]; Brain Korea 21 (BK21) ProjectMinistry of Education & Human Resources Development (MOEHRD), Republic of Korea	This work was supported by the Basic Science Research Programmes, 2018R1D1A1B07050781 to DHS and 2018R1 D1A1B07050942 to MK, funded by the National Research Foundation of Korea grant granted by the Ministry of Education, Science and Technology, Republic of Korea (MEST). S. Jo was supported by Brain Korea 21 (BK21) Project.	Burak M, 1999, TURK KLIN TIP BILIM, V19, P296; Castaneda-Ovando A, 2009, FOOD CHEM, V113, P859, DOI 10.1016/j.foodchem.2008.09.001; Chuck CP, 2011, PLOS ONE, V6, DOI 10.1371/journal.pone.0027228; de Wit E, 2016, NAT REV MICROBIOL, V14, P523, DOI 10.1038/nrmicro.2016.81; Donnelly CA, 2003, LANCET, V361, P1761, DOI 10.1016/S0140-6736(03)13410-1; Drosten C, 2003, NEW ENGL J MED, V348, P1967, DOI 10.1056/NEJMoa030747; Frabasile S, 2017, SCI REP-UK, V7, DOI 10.1038/srep41864; Friesner RA, 2006, J MED CHEM, V49, P6177, DOI 10.1021/jm051256o; Jacobson MP, 2004, PROTEINS, V55, P351, DOI 10.1002/prot.10613; Juca MM, 2020, NAT PROD RES, V34, P692, DOI 10.1080/14786419.2018.1493588; Kiat TS, 2006, BIOORG MED CHEM LETT, V16, P3337, DOI 10.1016/j.bmcl.2005.12.075; Kilianski A, 2013, J VIROL, V87, P11955, DOI 10.1128/JVI.02105-13; Kuo CJ, 2004, BIOCHEM BIOPH RES CO, V318, P862, DOI 10.1016/j.bbrc.2004.04.098; Lai MMC, 1997, ADV VIRUS RES, V48, P1, DOI 10.1016/S0065-3527(08)60286-9; Lau JTF, 2005, EMERG INFECT DIS, V11, P417; Lee YK, 2009, BRAIN RES, V1250, P164, DOI 10.1016/j.brainres.2008.10.012; Li WD, 2005, SCIENCE, V310, P676, DOI 10.1126/science.1118391; Lin H, 2009, SPECTROCHIM ACTA A, V73, P936, DOI 10.1016/j.saa.2009.04.025; Liu YC, 2005, BIOCHEM BIOPH RES CO, V333, P194, DOI 10.1016/j.bbrc.2005.05.095; Mesel-Lemoine M, 2012, J VIROL, V86, P7577, DOI 10.1128/JVI.00269-12; Needle D, 2015, ACTA CRYSTALLOGR D, V71, P1102, DOI 10.1107/S1399004715003521; Pohjala L, 2012, MOLECULES, V17, P10774, DOI 10.3390/molecules170910774; Ren ZL, 2013, PROTEIN CELL, V4, P248, DOI 10.1007/s13238-013-2841-3; Ryu YB, 2010, BIOORGAN MED CHEM, V18, P7940, DOI 10.1016/j.bmc.2010.09.035; Schwarz S, 2014, PLANTA MED, V80, P177, DOI 10.1055/s-0033-1360277; Sherman W, 2006, J MED CHEM, V49, P534, DOI 10.1021/jm050540c; Thi THN, 2012, BIOTECHNOL LETT, V34, P831, DOI 10.1007/s10529-011-0845-8; Wu AD, 2015, VIRUS RES, V208, P56, DOI 10.1016/j.virusres.2015.05.018; Yang LY, 2017, NAT PROD RES, V31, P487, DOI 10.1080/14786419.2016.1190719; Zakaryan H, 2017, ARCH VIROL, V162, P2539, DOI 10.1007/s00705-017-3417-y	30	1	1	40	40	TAYLOR & FRANCIS LTD	ABINGDON	2-4 PARK SQUARE, MILTON PARK, ABINGDON OR14 4RN, OXON, ENGLAND	1475-6366	1475-6374		J ENZYM INHIB MED CH	J. Enzym. Inhib. Med. Chem.	JAN 1	2020	35	1					145	151		10.1080/14756366.2019.1690480			7	Biochemistry & Molecular Biology; Chemistry, Medicinal	Biochemistry & Molecular Biology; Pharmacology & Pharmacy	JM6VB	WOS:000496348600001	31724441	DOAJ Gold, Green Published			2020-04-01	
J	Luk, HKH; Li, X; Fung, J; Lau, SKP; Woo, PCY				Luk, Hayes K. H.; Li, Xin; Fung, Joshua; Lau, Susanna K. P.; Woo, Patrick C. Y.			Molecular epidemiology, evolution and phylogeny of SARS coronavirus	INFECTION GENETICS AND EVOLUTION			English	Review						SARS coronavirus; Molecular epidemiology; Evolution; Phylogeny	RESPIRATORY SYNDROME CORONAVIRUS; CHINESE HORSESHOE BATS; MIDDLE-EAST; GENOMIC CHARACTERIZATION; DIFFERENT GENOTYPES; REVEALS EVOLUTION; SPIKE PROTEIN; BETACORONAVIRUS; RECOMBINATION; DISCOVERY	Shortly after its emergence in southern China in 2002/2003, Severe Acute Respiratory Syndrome coronavirus (SARS-CoV) was confirmed to be the cause of SARS. Subsequently, SARS-related CoVs (SARSr-CoVs) were found in palm civets from live animal markets in Guangdong and in various horseshoe bat species, which were believed to be the ultimate reservoir of SARSr-CoV. Till November 2018, 339 SARSr-CoV genomes have been sequenced, including 274 from human, 18 from civets and 47 from bats [mostly from Chinese horseshoe bats (Rhinolophus sinicus), n = 30; and greater horseshoe bats (Rhinolophus ferrumequinum), n = 9]. The human SARS-CoVs and civet SARSr-CoVs were collected in 2003/2004, while bat SARSr-CoVs were continuously isolated in the past 13 years even after the cessation of the SARS epidemic. SARSr-CoVs belong to the subgenus Sarbecovirus (previously lineage B) of genus Betacoronavirus and occupy a unique phylogenetic position. Overall, it is observed that the SARSr-CoV genomes from bats in Yunnan province of China possess the highest nucleotide identity to those from civets. It is evident from both multiple alignment and phylogenetic analyses that some genes of a particular SARSr-CoV from bats may possess higher while other genes possess much lower nucleotide identity to the corresponding genes of SARSr-CoV from human/civets, resulting in the shift of phylogenetic position in different phylogenetic trees. Our current model on the origin of SARS is that the human SARS-CoV that caused the epidemic in 2002/2003 was probably a result of multiple recombination events from a number of SARSr-CoV ancestors in different horseshoe bat species.	[Luk, Hayes K. H.; Li, Xin; Fung, Joshua; Lau, Susanna K. P.; Woo, Patrick C. Y.] Univ Hong Kong, Dept Microbiol, Hong Kong, Peoples R China; [Lau, Susanna K. P.; Woo, Patrick C. Y.] Univ Hong Kong, State Key Lab Emerging Infect Dis, Hong Kong, Peoples R China; [Lau, Susanna K. P.; Woo, Patrick C. Y.] Univ Hong Kong, Carol Yu Ctr Infect, Hong Kong, Peoples R China; [Lau, Susanna K. P.; Woo, Patrick C. Y.] Zhejiang Univ, Collaborat Innovat Ctr Diag & Treatment Infect Di, Hangzhou 310006, Zhejiang, Peoples R China	Lau, SKP; Woo, PCY (reprint author), Univ Hong Kong, Queen Mary Hosp, State Key Lab Emerging Infect Dis, Dept Microbiol, Block T, Hong Kong, Peoples R China.	skplau@hku.hk; pcywoo@hku.hk	Woo, Patrick Chiu Yat/AAJ-6112-2020	Woo, Patrick Chiu Yat/0000-0001-9401-1832; Luk, Hayes Kam Hei/0000-0002-9831-2912	Theme-based Research Scheme, University Grant Committee [T11/707/15]; Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Diseases and Research Capability on Antimicrobial Resistance for Department of Health, HKSAR Government; University Development Fund, The University of Hong KongUniversity of Hong Kong	This work is partly supported by the Theme-based Research Scheme (Project No. T11/707/15), University Grant Committee; Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Diseases and Research Capability on Antimicrobial Resistance for Department of Health, HKSAR Government; and University Development Fund, The University of Hong Kong.	Campanella JJ, 2003, BMC BIOINFORMATICS, V4, DOI 10.1186/1471-2105-4-29; de Groot RJ, 2011, VIRUS TAXONOMY, P806; Drexler JF, 2010, J VIROL, V84, P11336, DOI 10.1128/JVI.00650-10; Ge XY, 2013, NATURE, V503, P535, DOI 10.1038/nature12711; Guan Y, 2003, SCIENCE, V302, P276, DOI 10.1126/science.1087139; He BA, 2014, J VIROL, V88, P7070, DOI 10.1128/JVI.00631-14; Herrewegh AAPM, 1998, J VIROL, V72, P4508, DOI 10.1128/JVI.72.5.4508-4514.1998; Hu B, 2017, PLOS PATHOG, V13, DOI 10.1371/journal.ppat.1006698; Hu D, 2018, EMERG MICROBES INFEC, V7, DOI 10.1038/s41426-018-0155-5; Hulswit RJG, 2016, ADV VIRUS RES, V96, P29, DOI 10.1016/bs.aivir.2016.08.004; Kan B, 2005, J VIROL, V79, P11892, DOI 10.1128/JVI.79.18.11892-11900.2005; Lau S. K. P., 2016, New Microbes and New Infections, V11, P54, DOI 10.1016/j.nmni.2016.02.011; Lau SKP, 2005, P NATL ACAD SCI USA, V102, P14040, DOI 10.1073/pnas.0506735102; Lau SKP, 2007, VIROLOGY, V367, P428, DOI 10.1016/j.virol.2007.06.009; Lau SKP, 2018, J INFECT DIS, V218, P197, DOI 10.1093/infdis/jiy018; Lau SKP, 2018, J VIROL, V92, DOI [10.1128/JVI.00265-18, 10.1128/jvi.00265-18]; Lau SKP, 2015, J VIROL, V89, P10532, DOI 10.1128/JVI.01048-15; Lau SKP, 2015, J VIROL, V89, P3076, DOI 10.1128/JVI.02420-14; Lau SKP, 2013, J VIROL, V87, P8638, DOI 10.1128/JVI.01055-13; Lau SKP, 2012, J VIROL, V86, P11906, DOI 10.1128/JVI.01305-12; Lau SKP, 2012, J VIROL, V86, P5481, DOI 10.1128/JVI.06927-11; Lau SKP, 2011, J VIROL, V85, P11325, DOI 10.1128/JVI.05512-11; Lau SKP, 2010, J VIROL, V84, P11385, DOI 10.1128/JVI.01121-10; Lau SKP, 2010, J VIROL, V84, P2808, DOI 10.1128/JVI.02219-09; Li WD, 2005, SCIENCE, V310, P676, DOI 10.1126/science.1118391; Li WH, 2005, EMBO J, V24, P1634, DOI 10.1038/sj.emboj.7600640; Liu DX, 2014, ANTIVIR RES, V109, P97, DOI 10.1016/j.antiviral.2014.06.013; Menachery VD, 2015, NAT MED, V21, P1508, DOI 10.1038/nm.3985; Muth D, 2018, SCI REP-UK, V8, DOI 10.1038/s41598-018-33487-8; Peiris JSM, 2003, LANCET, V361, P1319, DOI 10.1016/S0140-6736(03)13077-2; Song HD, 2005, P NATL ACAD SCI USA, V102, P2430, DOI 10.1073/pnas.0409608102; Tang XC, 2006, J VIROL, V80, P7481, DOI 10.1128/JVI.00697-06; Terada Y, 2014, PLOS ONE, V9, DOI 10.1371/journal.pone.0106534; Tu CC, 2004, EMERG INFECT DIS, V10, P2244, DOI 10.3201/eid1012.040520; Wang N, 2018, VIROL SIN, V33, P104, DOI 10.1007/s12250-018-0012-7; Woo PCY, 2007, J VIROL, V81, P1574, DOI 10.1128/JVI.02182-06; Woo PCY, 2006, J VIROL, V80, P7136, DOI 10.1128/JVI.00509-06; Woo PCY, 2006, VIROLOGY, V351, P180, DOI 10.1016/j.virol.2006.02.041; Woo PCY, 2017, EMERG MICROBES INFEC, V6, DOI 10.1038/emi.2017.37; Woo PCY, 2014, EMERG INFECT DIS, V20, P560, DOI 10.3201/eid2004.131769; Woo PCY, 2014, J VIROL, V88, P1318, DOI 10.1128/JVI.02351-13; Woo PCY, 2012, J VIROL, V86, P3995, DOI 10.1128/JVI.06540-11; World Health Organisation, 2004, SUMM PROB SARS CAS O, P2003; Wu ZG, 2016, J INFECT DIS, V213, P579, DOI 10.1093/infdis/jiv476; Yang L, 2013, EMERG INFECT DIS, V19, P989, DOI 10.3201/eid1906.121648; Yang XL, 2016, J VIROL, V90, P3253, DOI 10.1128/JVI.02582-15; Yang ZY, 2005, P NATL ACAD SCI USA, V102, P797, DOI 10.1073/pnas.0409065102; Zeng LP, 2016, J VIROL, V90, P6573, DOI 10.1128/JVI.03079-15; 2016, P NATL ACAD SCI USA, V113, P3048, DOI DOI 10.1073/PNAS.1517719113; 2005, J VIROL, V79, P884	50	3	3	25	40	ELSEVIER SCIENCE BV	AMSTERDAM	PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS	1567-1348	1567-7257		INFECT GENET EVOL	Infect. Genet. Evol.	JUL	2019	71						21	30		10.1016/j.meegid.2019.03.001			10	Infectious Diseases	Infectious Diseases	HU7JT	WOS:000465457800004	30844511	Bronze			2020-04-01	
J	Morse, JS; Lalonde, T; Xu, SQ; Liu, WR				Morse, Jared S.; Lalonde, Tyler; Xu, Shiqing; Liu, Wenshe Ray			Learning from the Past: Possible Urgent Prevention and Treatment Options for Severe Acute Respiratory Infections Caused by 2019-nCoV	CHEMBIOCHEM			English	Article						antiviral agents; coronavirus; 2019-nCoV; SARS; spike proteins; RdRp; 3CLpro	PAPAIN-LIKE PROTEASE; RECEPTOR-BINDING DOMAIN; SARS-CORONAVIRUS; AURINTRICARBOXYLIC ACID; SPIKE PROTEIN; COV; VACCINE; POTENT; IDENTIFICATION; RIBAVIRIN	With the current trajectory of the 2019-nCoV outbreak unknown, public health and medicinal measures will both be needed to contain spreading of the virus and to optimize patient outcomes. Although little is known about the virus, an examination of the genome sequence shows strong homology with its better-studied cousin, SARS-CoV. The spike protein used for host cell infection shows key nonsynonymous mutations that might hamper the efficacy of previously developed therapeutics but remains a viable target for the development of biologics and macrocyclic peptides. Other key drug targets, including RNA-dependent RNA polymerase and coronavirus main proteinase (3CLpro), share a strikingly high (>95 %) homology to SARS-CoV. Herein, we suggest four potential drug candidates (an ACE2-based peptide, remdesivir, 3CLpro-1 and a novel vinylsulfone protease inhibitor) that could be used to treat patients suffering with the 2019-nCoV. We also summarize previous efforts into drugging these targets and hope to help in the development of broad-spectrum anti-coronaviral agents for future epidemics.	[Morse, Jared S.; Lalonde, Tyler; Xu, Shiqing; Liu, Wenshe Ray] Texas A&M Univ, Dept Chem, Texas A&M Drug Discovery Lab, College Stn, TX 77843 USA	Xu, SQ; Liu, WR (reprint author), Texas A&M Univ, Dept Chem, Texas A&M Drug Discovery Lab, College Stn, TX 77843 USA.	shiqing.xu@tamu.edu; wliu@chem.tamu.edu	Liu, wenshe/O-4425-2014	Liu, wenshe/0000-0002-7078-6534	National Institutes of HealthUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [R01GM121584, R01GM127575]; Cancer Prevention & Research Institute of Texas [RP170797]; Welch FoundationThe Welch Foundation [A1715]	Research support in the Texas A&M Drug Discovery Center is provided by the National Institutes of Health (grants R01GM121584 and R01GM127575), the Cancer Prevention & Research Institute of Texas (grant RP170797), and the Welch Foundation (grant A1715). We thank Sunshine Zea Leeuwon for providing assistance in designing all the figures.	Agostini ML, 2018, MBIO, V9, DOI 10.1128/mBio.00221-18; Al-Tawfiq JA, 2014, INT J INFECT DIS, V20, P42, DOI 10.1016/j.ijid.2013.12.003; [Anonymous], 2019, ANGEW CHEM, V131, P16051; Baez-Santos YM, 2015, ANTIVIR RES, V115, P21, DOI 10.1016/j.antiviral.2014.12.015; Baranov PV, 2005, VIROLOGY, V332, P498, DOI 10.1016/j.virol.2004.11.038; Cavanagh D, 2003, AVIAN PATHOL, V32, P567, DOI 10.1080/03079450310001621198; Chen Xin, 2009, Antivir Chem Chemother, V19, P151; Cheng KW, 2015, ANTIVIR RES, V115, P9, DOI 10.1016/j.antiviral.2014.12.011; Chiou HE, 2005, CHEST, V128, P263, DOI 10.1378/chest.128.1.263; CUSHMAN M, 1991, J MED CHEM, V34, P329, DOI 10.1021/jm00105a052; Du LY, 2009, NAT REV MICROBIOL, V7, P226, DOI 10.1038/nrmicro2090; Ferron F, 2018, P NATL ACAD SCI USA, V115, pE162, DOI 10.1073/pnas.1718806115; GONZALEZ RG, 1979, BIOCHIM BIOPHYS ACTA, V562, P534, DOI 10.1016/0005-2787(79)90116-3; Han DP, 2006, VIROLOGY, V350, P15, DOI 10.1016/j.virol.2006.01.029; He RT, 2004, BIOCHEM BIOPH RES CO, V320, P1199, DOI 10.1016/j.bbrc.2004.06.076; He YX, 2004, BIOCHEM BIOPH RES CO, V324, P773, DOI 10.1016/j.bbrc.2004.09.106; Hipolito CJ, 2012, CURR OPIN CHEM BIOL, V16, P196, DOI 10.1016/j.cbpa.2012.02.014; Holmes KV, 2003, J CLIN INVEST, V111, P1605, DOI [10.1172/JCI200318819, 10.1172/JCI18819]; Hu HB, 2005, J COMB CHEM, V7, P648, DOI 10.1021/cc0500607; Jiang SB, 2005, EMERG INFECT DIS, V11, P1016, DOI 10.3201/1107.050219; Kim Y, 2016, PLOS PATHOG, V12, DOI 10.1371/journal.ppat.1005531; Kim Y, 2015, J VIROL, V89, P4942, DOI 10.1128/JVI.03688-14; Kirchdoerfer RN, 2019, NAT COMMUN, V10, DOI 10.1038/s41467-019-10280-3; Kumar V, 2017, ANTIVIR RES, V141, P101, DOI 10.1016/j.antiviral.2017.02.007; Lee TW, 2005, J MOL BIOL, V353, P1137, DOI 10.1016/j.jmb.2005.09.004; Li CC, 2019, DRUG DISCOV TODAY, V24, P726, DOI 10.1016/j.drudis.2019.01.018; Li F, 2005, SCIENCE, V309, P1864, DOI 10.1126/science.1116480; Liu W, 2014, BIOORGAN MED CHEM, V22, P292, DOI 10.1016/j.bmc.2013.11.028; MCLAFFERTY MA, 1993, GENE, V128, P29, DOI 10.1016/0378-1119(93)90149-W; Muller MP, 2007, PHARMACOTHERAPY, V27, P494, DOI 10.1592/phco.27.4.494; Navas-Martin S, 2004, J NEUROVIROL, V10, P75, DOI 10.1080/13550280490280292; ONEIL KT, 1992, PROTEINS, V14, P509, DOI 10.1002/prot.340140411; Palei S, 2019, CHEMBIOCHEM, V20, P72, DOI 10.1002/cbic.201800552; Park JY, 2016, J ENZYM INHIB MED CH, V31, P23, DOI 10.3109/14756366.2014.1003215; Park JY, 2012, BIOL PHARM BULL, V35, P2036, DOI 10.1248/bpb.b12-00623; Ratia K, 2014, PLOS PATHOG, V10, DOI 10.1371/journal.ppat.1004113; Roper RL, 2009, EXPERT REV VACCINES, V8, P887, DOI [10.1586/erv.09.43, 10.1586/ERV.09.43]; Sheahan TP, 2017, SCI TRANSL MED, V9, DOI 10.1126/scitranslmed.aal3653; Smith EC, 2013, PLOS PATHOG, V9, DOI 10.1371/journal.ppat.1003565; Song YH, 2014, BIOL PHARM BULL, V37, P1021, DOI 10.1248/bpb.b14-00026; Stockman LJ, 2006, PLOS MED, V3, P1525, DOI 10.1371/journal.pmed.0030343; Taylor DR, 2006, VACCINE, V24, P863, DOI 10.1016/j.vaccine.2005.08.102; Wang XSS, 2019, ANGEW CHEM INT EDIT, V58, P15904, DOI 10.1002/anie.201908713; Xiao WW, 2010, MOL CANCER THER, V9, P2714, DOI 10.1158/1535-7163.MCT-10-0308; Xu X, 2003, NUCLEIC ACIDS RES, V31, P7117, DOI 10.1093/nar/gkg916; Yap YL, 2005, COMPUT BIOL CHEM, V29, P212, DOI 10.1016/j.compbiolchem.2005.04.006; Zhou Y, 2015, ANTIVIR RES, V116, P76, DOI 10.1016/j.antiviral.2015.01.011; Ziebuhr J, 2000, J GEN VIROL, V81, P853, DOI 10.1099/0022-1317-81-4-853	48	3	3	38	38	WILEY-V C H VERLAG GMBH	WEINHEIM	POSTFACH 101161, 69451 WEINHEIM, GERMANY	1439-4227	1439-7633		CHEMBIOCHEM	ChemBioChem	MAR 2	2020	21	5					730	738		10.1002/cbic.202000047		FEB 2020	9	Biochemistry & Molecular Biology; Chemistry, Medicinal	Biochemistry & Molecular Biology; Pharmacology & Pharmacy	KR0RR	WOS:000515400100001	32022370	Bronze			2020-04-01	
J	Stoecklin, SB; Rolland, P; Silue, Y; Mailles, A; Campese, C; Simondon, A; Mechain, M; Meurice, L; Nguyen, M; Bassi, C; Yamani, E; Behillil, S; Ismael, S; Nguyen, D; Malvy, D; Lescure, FX; Georges, S; Lazarus, C; Tabai, A; Stempfelet, M; Enouf, V; Coignard, B; Levy-Bruhl, D; Filleul, L; Vandentorren, S; Spaccaferri, G; Prouvost, H; Albert, M; Barbet, M; Brisebarre, A; Donati, F; van der Werf, S; Ardoin, A; Dreyer, M; Tararbit, K; Amodeo, M; Couaillier, E; Fabre, P; Ha Bold, D; Che, D; Bendjelloul, G; Lallemant, MB; Charachon, V; Deconinck, L; Descamps, D; Gerard, S; Houhou, N; Le Hingrat, Q; Lolom, I; Lucet, JC; Pourbaix, A; Valayer, S; Yazdanpana, Y; Boyer, A; Clouzeau, B; Combes, X; Desclaux, A; Dindart, JM; Duvignaud, A; Garrigue, I; Gruson, D; Lafon, ME; Perreau, P; Pistone, T; Poteau, M; Tentillier, E; Mathieu, P				Stoecklin, Sibylle Bernard; Rolland, Patrick; Silue, Yassoungo; Mailles, Alexandra; Campese, Christine; Simondon, Anne; Mechain, Matthieu; Meurice, Laure; Nguyen, Mathieu; Bassi, Clement; Yamani, Estelle; Behillil, Sylvie; Ismael, Sophie; Nguyen, Duc; Malvy, Denis; Lescure, Francois Xavier; Georges, Scarlett; Lazarus, Clement; Tabai, Anouk; Stempfelet, Morgane; Enouf, Vincent; Coignard, Bruno; Levy-Bruhl, Daniel; Filleul, Laurent; Vandentorren, Stephanie; Spaccaferri, Guillaume; Prouvost, Helene; Albert, Melanie; Barbet, Marion; Brisebarre, Angela; Donati, Flora; van der Werf, Sylvie; Ardoin, Alexis; Dreyer, Marion; Tararbit, Karim; Amodeo, Matthieu; Couaillier, Elodie; Fabre, Pascal; Ha Bold, Daniel; Che, Didier; Bendjelloul, Gisele; Lallemant, Marine Billaudelle; Charachon, Valentine; Deconinck, Laurene; Descamps, Diane; Gerard, Sandrine; Houhou, Nadirha; Le Hingrat, Quentin; Lolom, Isabelle; Lucet, Jean-Christophe; Pourbaix, Annabelle; Valayer, Simon; Yazdanpana, Yazdan; Boyer, Alexandre; Clouzeau, Benjamin; Combes, Xavier; Desclaux, Arnaud; Dindart, Jean-Michel; Duvignaud, Alexandre; Garrigue, Isabelle; Gruson, Didier; Lafon, Marie-Edith; Perreau, Pauline; Pistone, Thierry; Poteau, Maxime; Tentillier, Eric; Mathieu, Pauline		Invest Team	First cases of coronavirus disease 2019 (COVID-19) in France: surveillance, investigations and control measures, January 2020	EUROSURVEILLANCE			English	Article								A novel coronavirus (severe acute respiratory syndrome coronavirus 2, SARS-CoV-2) causing a cluster of respiratory infections (coronavirus disease 2019, COVID-19) in Wuhan, China, was identified on 7 January 2020. The epidemic quickly disseminated from Wuhan and as at 12 February 2020, 45,179 cases have been confirmed in 25 countries, including 1,116 deaths. Strengthened surveillance was implemented in France on 10 January 2020 in order to identify imported cases early and prevent secondary transmission. Three categories of risk exposure and follow-up procedure were defined for contacts. Three cases of COVID-19 were confirmed on 24 January, the first cases in Europe. Contact tracing was immediately initiated. Five contacts were evaluated as at low risk of exposure and 18 at moderate/high risk. As at 12 February 2020, two cases have been discharged and the third one remains symptomatic with a persistent cough, and no secondary transmission has been identified. Effective collaboration between all parties involved in the surveillance and response to emerging threats is required to detect imported cases early and to implement adequate control measures.	[Stoecklin, Sibylle Bernard; Mailles, Alexandra; Campese, Christine; Georges, Scarlett; Coignard, Bruno; Levy-Bruhl, Daniel; Che, Didier] Sante Publ France, Direct Malad Infect, St Maurice, France; [Rolland, Patrick] Sante Publ France, Direct Reg, St Maurice, France; [Silue, Yassoungo; Bassi, Clement] Sante Publ France, Direct Reg, Cellule Reg Ile France, Paris, France; [Simondon, Anne; Yamani, Estelle; van der Werf, Sylvie; Ardoin, Alexis; Dreyer, Marion; Tararbit, Karim] Agence Reg Sante Ile France, Paris, France; [Mechain, Matthieu; Nguyen, Mathieu; Amodeo, Matthieu; Couaillier, Elodie; Fabre, Pascal; Ha Bold, Daniel] Agence Reg Sante Nouvelle Aquitaine, Bordeaux, France; [Meurice, Laure; Filleul, Laurent; Vandentorren, Stephanie] Sante Publ France, Direct Reg, Cellule Reg Nouvelle Aquitaine, Bordeaux, France; [Behillil, Sylvie; Enouf, Vincent; Albert, Melanie; Barbet, Marion; Brisebarre, Angela; Donati, Flora; van der Werf, Sylvie] Inst Pasteur, Ctr Natl Reference Virus Infect Resp, Dont Grippe, Paris, France; [Ismael, Sophie; Lescure, Francois Xavier; Bendjelloul, Gisele; Lallemant, Marine Billaudelle; Charachon, Valentine; Deconinck, Laurene; Descamps, Diane; Gerard, Sandrine; Houhou, Nadirha; Le Hingrat, Quentin; Lolom, Isabelle; Lucet, Jean-Christophe; Pourbaix, Annabelle; Valayer, Simon; Yazdanpana, Yazdan] Hop Bichat Claude Bernard, AP HP, Serv Malad Infect & Trop, Paris, France; [Nguyen, Duc; Malvy, Denis] CHU Bordeaux, Serv Malad Infect & Trop, Bordeaux GeoSentinel Site, Bordeaux, France; [Malvy, Denis] Univ Bordeaux, UMR 1219, Bordeaux, France; [Lescure, Francois Xavier] Univ Paris, INSERM, IAME, Paris, France; [Lazarus, Clement; Mathieu, Pauline] Minist Solidarites & Sante, Direct Gen Sante, Ctr Operationnel Recept & Regulat Urgences Sanit, Paris, France; [Tabai, Anouk; Stempfelet, Morgane] Sante Publ France, Direct Alerte & Crise, St Maurice, France; [Spaccaferri, Guillaume] Sante Publ France, Direct Reg, Cellule Reg Auvergne Rhone Alpes, Lyon, France; [Prouvost, Helene] Sante Publ France, Direct Reg, Cellule Reg Hauts France, Lille, France; [Boyer, Alexandre; Clouzeau, Benjamin; Combes, Xavier; Desclaux, Arnaud; Dindart, Jean-Michel; Duvignaud, Alexandre; Garrigue, Isabelle; Gruson, Didier; Lafon, Marie-Edith; Perreau, Pauline; Pistone, Thierry; Poteau, Maxime; Tentillier, Eric] CHU Bordeaux, Bordeaux GeoSentinel Site, Bordeaux, France	Stoecklin, SB (reprint author), Sante Publ France, Direct Malad Infect, St Maurice, France.	sibylle.bernard-stoecklin@santepubUquefrance.fr	Ribeiro, Nuno/AAH-2299-2020	ARDOIN, Alexis/0000-0001-9506-5672			Chan JFW, 2020, LANCET, V395, P514, DOI 10.1016/S0140-6736(20)30154-9; Corman VM, 2020, EUROSURVEILLANCE, V25, P23, DOI 10.2807/1560-7917.ES.2020.25.3.2000045; ECDC, 2020, NOV COR; European Centre for Disease Prevention and Control (ECDC), 2020, OUTBR AC RESP SYNDR; European Centre for Disease Prevention and Control (ECDC), 2020, RISK ASS GUID INF DI; European Centre for Disease Prevention and Control (ECDC), 2020, PUBL HLTH MAN PERS H; Haut Conseil de la sante publique (HCSP), 2015, DEF CLASS CAS POSS C; Imai N., 2020, 3 IMP COLL LOND; Li Qun, 2020, N Engl J Med, V382, P1199, DOI 10.1056/NEJMoa2001316; Liu T, 2020, TRANSMISSION DYNAMIC, V2020, P2025; Riou J, PATTERN EARLY HUMAN; World Health Organization (WHO), 2020, 1 FEW X FFX CAS CONT; World Health Organization (WHO), GO DAT MAN COMPL DAT; World Health Organization ( WHO), 2020, NOV COR CHIN DIS OUT; Wu JT, 2020, LANCET	15	1	1	38	38	EUR CENTRE DIS PREVENTION & CONTROL	STOCKHOLM	TOMTEBODAVAGEN 11A, STOCKHOLM, 171 83, SWEDEN	1560-7917			EUROSURVEILLANCE	Eurosurveillance	FEB 13	2020	25	6					20	26	2000094	10.2807/1560-7917.ES.2020.25.6.2000094			7	Infectious Diseases	Infectious Diseases	KM9CR	WOS:000514439600004	32070465	DOAJ Gold, Green Published			2020-04-01	
J	Yoo, JH				Yoo, Jin-Hong			The Fight against the 2019-nCoV Outbreak: an Arduous March Has Just Begun	JOURNAL OF KOREAN MEDICAL SCIENCE			English	Editorial Material									[Yoo, Jin-Hong] Catholic Univ Korea, Coll Med, Dept Internal Med, Div Infect Dis, 327 Sosa Ro, Seoul 14647, South Korea; [Yoo, Jin-Hong] Bucheon St Marys Hosp, Div Infect Dis, Dept Internal Med, Bucheon, South Korea	Yoo, JH (reprint author), Catholic Univ Korea, Coll Med, Dept Internal Med, Div Infect Dis, 327 Sosa Ro, Seoul 14647, South Korea.	jhyoo@catholic.ac.kr		Yoo, Jin-Hong/0000-0003-2611-3399			[Anonymous], 2020, BNO NEWS; Chan JF-W, 2020, LANCET; Cui J, 2019, NAT REV MICROBIOL, V17, P181, DOI 10.1038/s41579-018-0118-9; Huang C, 2020, LANCET; Kang CK, 2017, J KOREAN MED SCI, V32, P744, DOI 10.3346/jkms.2017.32.5.744; Menachery VD, 2015, NAT MED, V21, P1508, DOI 10.1038/nm.3985; Munster V.J, 2020, N ENGL J MED; World Health Organization, 2020, WHO ISS BEST PRACT N; World Health Organization, 2020, SURV CAS DEF HUM INF; Zhu N, 2019, N ENGL J MED	10	5	5	38	38	KOREAN ACAD MEDICAL SCIENCES	SEOUL	302 75 DONG DU ICHON, DONG YONGSAN KU, SEOUL 140 031, SOUTH KOREA	1011-8934	1598-6357		J KOREAN MED SCI	J. Korean Med. Sci.	FEB 3	2020	35	4							e56	10.3346/jkms.2020.35.e56			3	Medicine, General & Internal	General & Internal Medicine	KJ4QY	WOS:000512044700008	31997618	DOAJ Gold, Green Published			2020-04-01	
J	Weinhart, M; Hocke, A; Hippenstiel, S; Kurreck, J; Hedtrich, S				Weinhart, Marie; Hocke, Andreas; Hippenstiel, Stefan; Kurreck, Jens; Hedtrich, Sarah			3D organ models-Revolution in pharmacological research?	PHARMACOLOGICAL RESEARCH			English	Review						Organ model; Pharmacological testing in vitro; 3D printing; Excised human tissue; Alternatives to animal testing; Tissue engineering	RESPIRATORY SYNDROME CORONAVIRUS; MESENCHYMAL STEM-CELLS; IN-VITRO; ATOPIC-DERMATITIS; LIVER SLICES; SKIN; LUNG; DIFFERENTIATION; INFLAMMATION; REPLICATION	3D organ models have gained increasing attention as novel preclinical test systems and alternatives to animal testing. Over the years, many excellent in vitro tissue models have been developed. In parallel, microfluidic organ-on-a-chip tissue cultures have gained increasing interest for their ability to house several organ models on a single device and interlink these within a human-like environment. In contrast to these advancements, the development of human disease models is still in its infancy. Although major advances have recently been made, efforts still need to be intensified. Human disease models have proven valuable for their ability to closely mimic disease patterns in vitro, permitting the study of pathophysiological features and new treatment options. Although animal studies remain the gold standard for preclinical testing, they have major drawbacks such as high cost and ongoing controversy over their predictive value for several human conditions. Moreover, there is growing political and social pressure to develop alternatives to animal models, clearly promoting the search for valid, cost-efficient and easy-to-handle systems lacking interspecies-related differences. In this review, we discuss the current state of the art regarding 3D organ as well as the opportunities, limitations and future implications of their use.	[Weinhart, Marie] Free Univ Berlin, Inst Chem & Biochem, Berlin, Germany; [Hocke, Andreas; Hippenstiel, Stefan] Charite Univ Med Berlin, Dept Infect & Resp Dis, Berlin, Germany; [Hocke, Andreas; Hippenstiel, Stefan] Free Univ Berlin, Berlin, Germany; [Hocke, Andreas; Hippenstiel, Stefan] Humboldt Univ, Berlin, Germany; [Hocke, Andreas; Hippenstiel, Stefan] Berlin Inst Hlth, Berlin, Germany; [Kurreck, Jens] Tech Univ Berlin, Inst Biotechnol, Berlin, Germany; [Hedtrich, Sarah] Free Univ Berlin, Inst Pharm Pharmacol & Toxicol, Konigin Luise Str 2-4, D-14195 Berlin, Germany	Hedtrich, S (reprint author), Free Univ Berlin, Inst Pharm Pharmacol & Toxicol, Konigin Luise Str 2-4, D-14195 Berlin, Germany.	sarah.hedtrich@fu-berlin.de	Kurreck, Jens/B-7785-2008; Weinhart, Marie/D-1696-2018	Kurreck, Jens/0000-0002-1469-0052; Hedtrich (nee Kuchler), Sarah/0000-0001-6770-3657	Berlin-Brandenburg research platform BB3R; "Bundesinstitut fur Risikobewertung" (BfR) [1328-566]; German Research FoundationGerman Research Foundation (DFG) [DFG SFB-TR84]; German Federal Ministry of Education and Research (BMBF, RAPID-Network); German Federal Ministry of Education and ResearchFederal Ministry of Education & Research (BMBF) [FKZ13N13523]; "Stiftung zur Forderung der Erforschung von Ersatz-und Erganzungsmethoden zur Einschrankung von Tierversuchen" (Stiftung SET); Bundesinstitut fur Risikobewertung [1328-568]	Financial support by the Berlin-Brandenburg research platform BB3R and the "Bundesinstitut fur Risikobewertung" (BfR; 1328-566) is gratefully acknowledged by S.He. This work was also supported by the German Research Foundation (DFG SFB-TR84) to A.C.H. and S.Hi. (B6 and TF1) and A.C.H (Z1a) and the German Federal Ministry of Education and Research (BMBF, RAPID-Network to A.C.H. and S.Hi and FKZ13N13523 to M.W.). Further financial support to J.K. from the "Stiftung zur Forderung der Erforschung von Ersatz-und Erganzungsmethoden zur Einschrankung von Tierversuchen" (Stiftung SET) and the "Bundesinstitut fur Risikobewertung" (1328-568) is gratefully acknowledged. Moreover, we thank Dr. Andrea Marzoll and Dr. Roland Frotschl from the Federal Institute for Drugs and Medical Devices in Germany for their valuable help and insights in decision processes of regulatory authorities. Dr. Guy Yealland's help in language editing is gratefully acknowledged and we are thankful to Johanna Berg, Thomas Hiller and Anna Lowa for providing images of 3D printed models and figure design.	Bai Y, 2016, AM J RESP CELL MOL, V54, P656, DOI 10.1165/rcmb.2015-0290MA; Berg J, 2018, SCI REP-UK, V8, DOI 10.1038/s41598-018-31880-x; Berg J, 2017, EUR RESPIR J, V50, DOI 10.1183/13993003.01953-2016; Berroth A, 2013, J ALLERGY CLIN IMMUN, V131, P1547, DOI 10.1016/j.jaci.2013.02.029; Blunder S, 2017, J INVEST DERMATOL, V137, P706, DOI 10.1016/j.jid.2016.09.034; Caddeo S, 2017, FRONT BIOENG BIOTECH, V5, DOI 10.3389/fbioe.2017.00040; Chan RWY, 2014, LANCET RESP MED, V2, P813, DOI 10.1016/S2213-2600(14)70158-4; Choi YJ, 2018, J MATER CHEM B, V6, DOI 10.1039/c8tb01475b; Couzin-Frankel J, 2013, SCIENCE, V342, P922, DOI 10.1126/science.342.6161.922; Crespo M, 2017, NAT MED, V23, P878, DOI 10.1038/nm.4355; Danso MO, 2014, J INVEST DERMATOL, V134, P1941, DOI 10.1038/jid.2014.83; Desmet E, 2017, EXP BIOL MED, V242, P1158, DOI 10.1177/1535370217710637; DesRochers TM, 2013, PLOS ONE, V8, DOI 10.1371/journal.pone.0059219; Duque-Fernandez Alexandra, 2016, Biochem Biophys Rep, V8, P268, DOI 10.1016/j.bbrep.2016.09.012; Eckl KM, 2014, BRIT J DERMATOL, V171, P1289, DOI 10.1111/bjd.13450; Essaidi-Laziosi M, 2018, J ALLERGY CLIN IMMUN, V141, P2074, DOI 10.1016/j.jaci.2017.07.018; Ewald DA, 2017, J ALLERGY CLIN IMMUN, V139, P562, DOI 10.1016/j.jaci.2016.08.029; Fatehullah A, 2016, NAT CELL BIOL, V18, P246, DOI 10.1038/ncb3312; Faway E, 2017, MED MYCOL, V55, P485, DOI 10.1093/mmy/myw111; Fytianos K, 2017, ACS NANO, V11, P375, DOI 10.1021/acsnano.6b06061; Gerber PA, 2014, BIOL CHEM, V395, P577, DOI 10.1515/hsz-2013-0279; Gnadt M, 2012, EUR J PHARM BIOPHARM, V81, P617, DOI 10.1016/j.ejpb.2012.04.013; Gordon S, 2015, ALTEX-ALTERN ANIM EX, V32, P327, DOI 10.14573/altex.1510051; Harrington H, 2014, MOL PHARMACEUT, V11, P2082, DOI 10.1021/mp5000295; Hedstrom U, 2018, SCI REP-UK, V8, DOI 10.1038/s41598-018-21727-w; Hocke AC, 2013, AM J RESP CRIT CARE, V188, P882, DOI 10.1164/rccm.201305-0954LE; Honzke S, 2016, J CONTROL RELEASE, V242, P50, DOI 10.1016/j.jconrel.2016.06.030; Honzke S, 2016, J INVEST DERMATOL, V136, P631, DOI 10.1016/j.jid.2015.11.007; Homan KA, 2016, SCI REP-UK, V6, DOI 10.1038/srep34845; Hopkins AM, 2015, PROG NEUROBIOL, V125, P1, DOI 10.1016/j.pneurobio.2014.11.003; Horvath L, 2015, SCI REP-UK, V5, DOI 10.1038/srep07974; Huber B, 2016, TISSUE ENG PART C-ME, V22, P756, DOI [10.1089/ten.tec.2016.0141, 10.1089/ten.TEC.2016.0141]; Johnson BP, 2018, BIOTECHNIQUES, V64, P13, DOI 10.2144/000114626; Kim BS, 2018, BIOMATERIALS, V168, P38, DOI 10.1016/j.biomaterials.2018.03.040; Kress S, 2018, SCI REP-UK, V8, DOI 10.1038/s41598-018-22688-w; Lagaye S, 2012, HEPATOLOGY, V56, P861, DOI 10.1002/hep.25738; Lee HP, 2017, NAT MATER, V16, P1243, DOI [10.1038/NMAT4993, 10.1038/nmat4993]; Lee JW, 2013, AM J RESP CRIT CARE, V187, P751, DOI 10.1164/rccm.201206-0990OC; Lee PS, 2017, TISSUE ENG PART C-ME, V23, P286, DOI [10.1089/ten.tec.2016.0244, 10.1089/ten.TEC.2016.0244]; Leist M, 2013, ARCH TOXICOL, V87, P563, DOI 10.1007/s00204-013-1038-0; Ma XY, 2016, P NATL ACAD SCI USA, V113, P2206, DOI 10.1073/pnas.1524510113; Marrazzo P, 2016, PLOS ONE, V11, DOI 10.1371/journal.pone.0153985; Maschmeyer I, 2015, LAB CHIP, V15, P2688, DOI 10.1039/c5lc00392j; Mazza G, 2017, SCI REP-UK, V7, DOI 10.1038/s41598-017-05134-1; McAuley DF, 2014, AM J PHYSIOL-LUNG C, V306, pL809, DOI 10.1152/ajplung.00358.2013; Nelson CM, 2017, DEVELOPMENT, V144, P4328, DOI 10.1242/dev.154823; Netland J, 2008, J VIROL, V82, P7264, DOI 10.1128/JVI.00737-08; Ng WL, 2018, BIOFABRICATION, V10, DOI 10.1088/1758-5090/aa9e1e; Ng WL, 2016, TRENDS BIOTECHNOL, V34, P689, DOI 10.1016/j.tibtech.2016.04.006; Nguyen DG, 2016, PLOS ONE, V11, DOI 10.1371/journal.pone.0158674; Nichols JE, 2014, EXP BIOL MED, V239, P1135, DOI 10.1177/1535370214536679; Norona LM, 2016, TOXICOL SCI, V154, P354, DOI 10.1093/toxsci/kfw169; Osuchowski MF, 2014, SHOCK, V41, P463, DOI 10.1097/SHK.0000000000000153; Park JA, 2017, SCI REP-UK, V7, DOI 10.1038/s41598-017-14726-w; Pati F, 2014, NAT COMMUN, V5, DOI 10.1038/ncomms4935; Perrin S, 2014, NATURE, V507, P423, DOI 10.1038/507423a; Pound P, 2014, BMJ-BRIT MED J, V348, DOI 10.1136/bmj.g3387; Pridgeon CS, 2018, ARCH TOXICOL, V92, P557, DOI 10.1007/s00204-018-2152-9; Ronaldson-Bouchard K, 2018, CELL STEM CELL, V22, P310, DOI 10.1016/j.stem.2018.02.011; Rubsam M, 2017, NAT COMMUN, V8, DOI 10.1038/s41467-017-01170-7; Sargent R. S., 2010, WINT SIM C; Saturni S, 2015, ALLERGY ASTHMA IMMUN, V7, P525, DOI 10.4168/aair.2015.7.6.525; Schweinlin M, 2017, DRUG DELIV TRANSL RE, V7, P217, DOI 10.1007/s13346-016-0316-9; Seok J, 2013, P NATL ACAD SCI USA, V110, P3507, DOI 10.1073/pnas.1222878110; Smits JPH, 2017, SCI REP-UK, V7, DOI 10.1038/s41598-017-12041-y; Takao K, 2015, P NATL ACAD SCI USA, V112, P1167, DOI 10.1073/pnas.1401965111; Temann A, 2017, HUM VACC IMMUNOTHER, V13, P351, DOI 10.1080/21645515.2017.1264794; Tsioptsias N, 2016, 5 STUD C OP RES SCOR; Valm AM, 2017, NATURE, V546, P162, DOI 10.1038/nature22369; Vickers AEM, 2017, INT J MOL SCI, V18, DOI 10.3390/ijms18030574; Wagner A, 2015, J BIOTECHNOL, V212, P134, DOI 10.1016/j.jbiotec.2015.08.012; Warnecke G, 2018, LANCET RESP MED, V6, P357, DOI 10.1016/S2213-2600(18)30136-X; Westra IM, 2016, TOXICOL IN VITRO, V35, P77, DOI 10.1016/j.tiv.2016.05.012; Wilkinson DC, 2017, STEM CELL TRANSL MED, V6, P622, DOI 10.5966/sctm.2016-0192; Wu X, 2018, J IMMUNOL METHODS, V455, P71, DOI 10.1016/j.jim.2018.01.012; Wufuer M, 2016, SCI REP-UK, V6, DOI 10.1038/srep37471; Yonker LM, 2017, SCI REP-UK, V7, DOI 10.1038/s41598-017-08567-w; Zoschke C, 2016, J CONTROL RELEASE, V233, P10, DOI 10.1016/j.jconrel.2016.04.037	78	7	8	11	38	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD	LONDON	24-28 OVAL RD, LONDON NW1 7DX, ENGLAND	1043-6618			PHARMACOL RES	Pharmacol. Res.	JAN	2019	139						446	451		10.1016/j.phrs.2018.11.002			6	Pharmacology & Pharmacy	Pharmacology & Pharmacy	HL4QU	WOS:000458709000042	30395949				2020-04-01	
J	Sheikh, A; Al-Taher, A; Al-Nazawi, M; Al-Mubarak, AI; Kandeel, M				Sheikh, Abdullah; Al-Taher, Abdulla; Al-Nazawi, Mohammed; Al-Mubarak, Abdullah, I; Kandeel, Mahmoud			Analysis of preferred codon usage in the coronavirus N genes and their implications for genome evolution and vaccine design	JOURNAL OF VIROLOGICAL METHODS			English	Article						Coronavirus; Nucleocapsid protein; Preferred nucleotides; Amino acid; Codon bias	SARS-ASSOCIATED CORONAVIRUS; NUCLEOCAPSID PROTEIN; MONOCLONAL-ANTIBODY; BACTERIAL GENES; SPIKE PROTEIN; PATTERNS; VIRUS; BIAS; PRESSURE; PURIFICATION	The nucleocapsid (N) protein of a coronavirus plays a crucial role in virus assembly and in its RNA transcription. It is important to characterize a virus at the nucleotide level to discover the virus's genomic sequence variations and similarities relative to other viruses that could have an impact on the functions of its genes and proteins. This entails a comprehensive and comparative analysis of the viral genomes of interest for preferred nucleotides, codon bias, nucleotide changes at the 3rd position (NT3s), synonymous codon usage and relative synonymous codon usage. In this study, the variations in the N proteins among 13 different coronaviruses (CoVs) were analysed at the nucleotide and amino acid levels in an attempt to reveal how these viruses adapt to their hosts relative to their preferred codon usage in the N genes. The results revealed that, overall, eighteen amino acids had different preferred codons and eight of these were over-biased. The N genes had a higher AT% over GC% and the values of their effective number of codons ranged from 40.43 to 53.85, indicating a slight codon bias. Neutrality plots and correlation analyses showed a very high level of GC3s/GC correlation in porcine epidemic diarrhea CoV (pedCoV), followed by Middle East respiratory syndrome-CoV (MERS CoV), porcine delta CoV (dCoV), bat CoV (bCoV) and feline CoV (fCoV) with r values 0.81, 0.68, -0.47, 0.98 and 0.58, respectively. These data implied a high rate of evolution of the CoV genomes and a strong influence of mutation on evolutionary selection in the CoV N genes. This type of genetic analysis would be useful for evaluating a virus's host adaptation, evolution and is thus of value to vaccine design strategies.	[Sheikh, Abdullah] King Faisal Univ, Camel Res Ctr, Alhofuf 31982, Alahsa, Saudi Arabia; [Al-Taher, Abdulla; Al-Nazawi, Mohammed; Kandeel, Mahmoud] King Faisal Univ, Coll Vet Med, Dept Biomed Sci, Alhofuf 31982, Alahsa, Saudi Arabia; [Al-Mubarak, Abdullah, I] King Faisal Univ, Coll Vet Med, Dept Microbiol, Alhofuf 31982, Alahsa, Saudi Arabia; [Kandeel, Mahmoud] Kafrelsheikh Univ, Fac Vet Med, Dept Pharmacol, Kafrelsheikh 33516, Egypt	Sheikh, A (reprint author), King Faisal Univ, Camel Res Ctr, Alhofuf 31982, Alahsa, Saudi Arabia.		Al-Taher, Abdulla/P-6246-2016; Kandeel, Mahmoud/G-3636-2016; Sheikh, Abdullah/X-7065-2018	Al-Taher, Abdulla/0000-0002-0831-6486; Kandeel, Mahmoud/0000-0003-3668-5147; Sheikh, Abdullah/0000-0002-0482-503X	Deanship of Scientific Research at King Faisal University [171001]	The authors acknowledge the Deanship of Scientific Research at King Faisal University for the financial support under strategic projects track [grant number 171001].	Ahn I, 2009, EXP MOL MED, V41, P746, DOI 10.3858/emm.2009.41.10.081; Bahir I, 2009, MOL SYST BIOL, V5, DOI 10.1038/msb.2009.71; Behura SK, 2013, BIOL REV, V88, P49, DOI 10.1111/j.1469-185X.2012.00242.x; Berry M., 2012, AFR J BIOTECHNOL, V11, P13962; Blanchard EG, 2011, J VIROL METHODS, V177, P100, DOI 10.1016/j.jviromet.2011.07.005; Cavanagh D., 1995, CORONAVIRIDAE, P73; Chaney JL, 2015, ANNU REV BIOPHYS, V44, P143, DOI 10.1146/annurev-biophys-060414-034333; Chantawannakul P, 2008, J INVERTEBR PATHOL, V98, P206, DOI 10.1016/j.jip.2008.02.016; Che XY, 2004, EMERG INFECT DIS, V10, P1947, DOI 10.3201/eid1011.040516; Chen SL, 2004, P NATL ACAD SCI USA, V101, P3480, DOI 10.1073/pnas.0307827100; Chen Y, 2014, INFECT GENET EVOL, V28, P95, DOI 10.1016/j.meegid.2014.09.004; Chen Youhua, 2014, VirusDisease, V25, P285, DOI 10.1007/s13337-014-0191-2; Cheng XF, 2012, VIROL J, V9, DOI 10.1186/1743-422X-9-113; Cristina J, 2015, VIRUS RES, V196, P87, DOI 10.1016/j.virusres.2014.11.005; D'Andrea L, 2011, VIRUS RES, V157, P19, DOI 10.1016/j.virusres.2011.01.012; Di B, 2005, CLIN DIAGN LAB IMMUN, V12, P135, DOI 10.1128/CDLI.12.1.135-140.2005; Doolittle WE, 1998, TRENDS GENET, V14, P307; Du LY, 2010, MICROBES INFECT, V12, P280, DOI 10.1016/j.micinf.2010.01.001; Dutta NK, 2008, IMMUNOL LETT, V118, P65, DOI 10.1016/j.imlet.2008.03.003; Fellenberg K, 2001, P NATL ACAD SCI USA, V98, P10781, DOI 10.1073/pnas.181597298; Gao J, 2013, J VIROL, V87, P13134, DOI 10.1128/JVI.02433-13; Greenacre M. J., 1984, THEORY APPL CORRES A; Gu WJ, 2004, VIRUS RES, V101, P155, DOI 10.1016/j.virusres.2004.01.006; He QG, 2005, J VIROL METHODS, V127, P46, DOI 10.1016/j.jviromet.2005.03.004; IKEMURA T, 1985, MOL BIOL EVOL, V2, P13; Jenkins GM, 2003, VIRUS RES, V92, P1, DOI 10.1016/S0168-1702(02)00309-X; Johnson R.F., 2019, U.S. Patent Application, Patent No. [16/091,005, 16091005]; Kanaya S, 2001, GENE, V276, P89, DOI 10.1016/S0378-1119(01)00673-4; Kandeel M, 2017, BIOL PHARM BULL, V40, P1086, DOI 10.1248/bpb.b17-00168; Kandeel Mahmoud, 2009, Nucleic Acids Symp Ser (Oxf), P39, DOI 10.1093/nass/nrp020; Kandeel Mahmoud, 2009, Nucleic Acids Symp Ser (Oxf), P283, DOI 10.1093/nass/nrp142; Kandeel M, 2009, BIOL PHARM BULL, V32, P1321, DOI 10.1248/bpb.32.1321; Karniychuk UU, 2016, VIRUS RES, V223, P73, DOI 10.1016/j.virusres.2016.06.015; Kattoor JJ, 2015, INFECT GENET EVOL, V34, P17, DOI 10.1016/j.meegid.2015.06.018; Kumar N, 2016, PLOS ONE, V11, DOI 10.1371/journal.pone.0154376; Lee HK, 2008, J MICROBIOL BIOTECHN, V18, P1717; Liang FY, 2013, J VIROL METHODS, V187, P413, DOI 10.1016/j.jviromet.2012.11.009; LLOYD AT, 1992, NUCLEIC ACIDS RES, V20, P5289, DOI 10.1093/nar/20.20.5289; Loa CC, 2004, J VIROL METHODS, V116, P161, DOI 10.1016/j.jviromet.2003.11.006; Ma YP, 2015, J FISH DIS, V38, P665, DOI 10.1111/jfd.12316; Maache M, 2006, CLIN VACCINE IMMUNOL, V13, P409, DOI 10.1128/CVI.13.3.409-414.2006; McBride R, 2014, VIRUSES-BASEL, V6, P2991, DOI 10.3390/v6082991; Nakamura Y, 2000, NUCLEIC ACIDS RES, V28, P292, DOI 10.1093/nar/28.1.292; Nasrullah I, 2015, BMC EVOL BIOL, V15, DOI 10.1186/s12862-015-0456-4; Neuman BW, 2011, J STRUCT BIOL, V174, P11, DOI 10.1016/j.jsb.2010.11.021; Ochman H, 2000, NATURE, V405, P299, DOI 10.1038/35012500; Peden J.F., 2000, ANAL CODON USAGE, P2000; Perriere G, 2002, NUCLEIC ACIDS RES, V30, P4548, DOI 10.1093/nar/gkf565; Risco C, 1996, J VIROL, V70, P4773, DOI 10.1128/JVI.70.7.4773-4777.1996; Ruch TR, 2012, VIRUSES-BASEL, V4, P363, DOI 10.3390/v4030363; SHARP PM, 1987, MOL BIOL EVOL, V4, P222; SHARP PM, 1993, BIOCHEM SOC T, V21, P835, DOI 10.1042/bst0210835; Shi SL, 2016, INFECT GENET EVOL, V44, P412, DOI 10.1016/j.meegid.2016.07.042; Shi SL, 2013, VIRUS GENES, V46, P10, DOI 10.1007/s11262-012-0818-6; Siddell S.G., 2005, TOPLEY WILSONS MICRO; SUEOKA N, 1988, P NATL ACAD SCI USA, V85, P2653, DOI 10.1073/pnas.85.8.2653; Sui JH, 2014, J VIROL, V88, P13769, DOI 10.1128/JVI.02232-14; Supek F, 2016, J MOL EVOL, V82, P65, DOI 10.1007/s00239-015-9714-8; Suzuki H, 2008, DNA RES, V15, P357, DOI 10.1093/dnares/dsn028; Timani KA, 2004, J CLIN VIROL, V30, P309, DOI 10.1016/j.jcv.2004.01.001; van Hemert F, 2016, J GEN VIROL, V97, P2608, DOI 10.1099/jgv.0.000579; Wasmoen T L, 1995, Adv Exp Med Biol, V380, P221; Woese CR, 2002, P NATL ACAD SCI USA, V99, P8742, DOI 10.1073/pnas.132266999; Wu G, 2005, J MOL MODEL, V11, P8, DOI 10.1007/s00894-004-0210-0; Wu HS, 2004, J BIOMED SCI, V11, P117, DOI [10.1159/000075294, 10.1007/BF02256554]; Wu XD, 2004, CELL RES, V14, P400, DOI 10.1038/sj.cr.7290240; Yu M, 2008, J IMMUNOL METHODS, V331, P1, DOI 10.1016/j.jim.2007.11.009; Zhou H, 2015, INFECT GENET EVOL, V35, P27, DOI 10.1016/j.meegid.2015.07.024; Zhou T, 2005, BIOSYSTEMS, V81, P77, DOI 10.1016/j.biosystems.2005.03.002	69	0	0	37	37	ELSEVIER	AMSTERDAM	RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS	0166-0934	1879-0984		J VIROL METHODS	J. Virol. Methods	MAR	2020	277								113806	10.1016/j.jviromet.2019.113806			8	Biochemical Research Methods; Biotechnology & Applied Microbiology; Virology	Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Virology	KM6NT	WOS:000514255500007	31911390	Bronze			2020-04-01	
J	Luo, H; Tang, QL; Shang, YX; Liang, SB; Yang, M; Robinson, N; Liu, JP				Luo Hui; Tang Qiao-ling; Shang Ya-xi; Liang Shi-bing; Yang Ming; Robinson, Nicola; Liu Jian-ping			Can Chinese Medicine Be Used for Prevention of Corona Virus Disease 2019 (COVID-19)? A Review of Historical Classics, Research Evidence and Current Prevention Programs	CHINESE JOURNAL OF INTEGRATIVE MEDICINE			English	Review; Early Access						Chinese medicine; corona virus disease 2019 (COVID-19); prevention program; clinical evidence; review	ACUTE RESPIRATORY SYNDROME	Objective Since December 2019, an outbreak of corona virus disease 2019 (COVID-19) occurred in Wuhan, and rapidly spread to almost all parts of China. This was followed by prevention programs recommending Chinese medicine (CM) for the prevention. In order to provide evidence for CM recommendations, we reviewed ancient classics and human studies. Methods Historical records on prevention and treatment of infections in CM classics, clinical evidence of CM on the prevention of severe acute respiratory syndrome (SARS) and H1N1 influenza, and CM prevention programs issued by health authorities in China since the COVID-19 outbreak were retrieved from different databases and websites till 12 February, 2020. Research evidence included data from clinical trials, cohort or other population studies using CM for preventing contagious respiratory virus diseases. Results The use of CM to prevent epidemics of infectious diseases was traced back to ancient Chinese practice cited in Huangdi's Internal Classic (Huang Di Nei Jing) where preventive effects were recorded. There were 3 studies using CM for prevention of SARS and 4 studies for H1N1 influenza. None of the participants who took CM contracted SARS in the 3 studies. The infection rate of H1N1 influenza in the CM group was significantly lower than the non-CM group (relative risk 0.36, 95% confidence interval 0.24-0.52; n=4). For prevention of COVID-19, 23 provinces in China issued CM programs. The main principles of CM use were to tonify qi to protect from external pathogens, disperse wind and discharge heat, and resolve dampness. The most frequently used herbs included Radix astragali (Huangqi), Radix glycyrrhizae (Gancao), Radix saposhnikoviae (Fangfeng), Rhizoma Atractylodis Macrocephalae (Baizhu), Lonicerae Japonicae Flos (Jinyinhua), and Fructus forsythia (Lianqiao). Conclusions Based on historical records and human evidence of SARS and H1N1 influenza prevention, Chinese herbal formula could be an alternative approach for prevention of COVID-19 in high-risk population. Prospective, rigorous population studies are warranted to confirm the potential preventive effect of CM.	[Luo Hui] China Tibetol Res Ctr, Inst Tibetan Med, Beijing 100101, Peoples R China; [Luo Hui; Shang Ya-xi; Liang Shi-bing; Yang Ming; Robinson, Nicola; Liu Jian-ping] Beijing Univ Chinese Med, Ctr Evidence Based Chinese Med, Beijing 100029, Peoples R China; [Tang Qiao-ling; Shang Ya-xi; Liang Shi-bing; Yang Ming] Beijing Univ Chinese Med, Sch Tradit Chinese Med, Beijing 100029, Peoples R China; [Robinson, Nicola] London South Bank Univ, Sch Hlth & Social Care, London SE1 0AA, England; [Liu Jian-ping] Guangzhou Med Univ, Inst Integrated Tradit Chinese Med & Western Med, Guangzhou 510120, Peoples R China	Liu, JP (reprint author), Beijing Univ Chinese Med, Ctr Evidence Based Chinese Med, Beijing 100029, Peoples R China.; Liu, JP (reprint author), Guangzhou Med Univ, Inst Integrated Tradit Chinese Med & Western Med, Guangzhou 510120, Peoples R China.	jianping_l@hotmail.com			National Natural Science Foundation of China, ChinaNational Natural Science Foundation of China [81830115]; Overseas Expertise Project, Ministry of Education of China [MS20080009]	Supported by the National Natural Science Foundation of China (No.81830115), China; Prof. Nicola Robinson (visiting professor of Beijing University of Chinese Medicine) is supported by the Overseas Expertise Project, Ministry of Education of China (No. MS20080009)	[Anonymous], 2007, GEN TREATISE FEBRILE; [Anonymous], 2020, FEB 12 DAIL BRIEF NO; [Anonymous], 2020, 22 WHO; [Anonymous], 2011, ESSENTIAL PRESCRIPTI; [Anonymous], 2004, SARS CLIN TRIALS TRE; Chen M, 2013, THESIS; Chen N, 2020, LANCET; Cheng KF, 2007, INT J INFECT DIS, V11, P360, DOI 10.1016/j.ijid.2006.07.009; Du CYQ, 2015, PHYTOTHER RES, V29, P656, DOI 10.1002/ptr.5290; Gao J, 2009, CHINESE MED J-PEKING, V122, P1636, DOI 10.3760/cma.j.issn.0366-6999.2009.14.007; Gao Y, 2020, EPIDEMIC DYNAMICS 20; Lau JTF, 2005, J ALTERN COMPLEM MED, V11, P49, DOI 10.1089/acm.2005.11.49; Liu B, 2010, TRADIT CHIN MED RES, V23, P46; Liu JP, 2004, J ALTERN COMPLEM MED, V10, P1041, DOI 10.1089/acm.2004.10.1041; Liu L, 2013, BEIJING J TRADIT CHI, V32, P91; Liu LiSong, 2015, International Journal of Biotechnology for Wellness Industries, V4, P57; National Administration of Traditional Chinese Medicine, 2009, CHIN COMM DOCTORS CH, V25, P13; National Health Commission of People's Republic of China, 2020, DIAGN TREATM PNEUM C; NEEDHAM J, 1962, J Hist Med Allied Sci, V17, P429; Ou AH, 2014, CHIN J INTEGR MED, V20, P101, DOI 10.1007/s11655-013-1582-8; Song Y, 2019, SHAANXI J TRADIT CHI, V40, P886; Su Y, 2005, J PRACT TRADIT CHIN, V21, P508; Wang, 2011, JILIN J TRADIT CHIN, V31, P197; Wang C, 2020, LANCET; Wang C, 2011, ANN INTERN MED, V155, P217, DOI 10.7326/0003-4819-155-4-201108160-00005; Wang D, 2020, JAMA; World Health Organization, 2020, Q A COR; Xia B, 2010, PEOPLES MILITARY SUR, V53, P645; Xu J, 2006, C PREV TREATM SARS I, P158; Yao W, 2009, THESIS; Yuan Y, 1990, J CHENGDU U TRADIT C, V13, P46; [钟燕春 Zhong Yanchun], 2011, [南京中医药大学学报, Journal of Nanjing University of Traditional Chinese Medicine], V27, P209; 张丽, 2005, [中国医院药学杂志, Chinese Journal of Hospital Pharmacy], V25, P59	33	0	0	37	37	SPRINGER	NEW YORK	ONE NEW YORK PLAZA, SUITE 4600, NEW YORK, NY, UNITED STATES	1672-0415	1993-0402		CHIN J INTEGR MED	Chin. J. Integr. Med.												10.1007/s11655-020-3192-6		FEB 2020	8	Integrative & Complementary Medicine	Integrative & Complementary Medicine	KP5FA	WOS:000516262000001	32065348	Bronze			2020-04-01	
J	Wang, ZW; Chen, XR; Lu, YF; Chen, FF; Zhang, W				Wang, Zhenwei; Chen, Xiaorong; Lu, Yunfei; Chen, Feifei; Zhang, Wei			Clinical characteristics and therapeutic procedure for four cases with 2019 novel coronavirus pneumonia receiving combined Chinese and Western medicine treatment	BIOSCIENCE TRENDS			English	Article						2019-nCoV; lopinavir; ritonavir; atbidol; Shufeng Jiedu Capsule		Pneumonia associated with the 2019 novel coronavirus (2019-nCoV) is continuously and rapidly circulating at present. No effective antiviral treatment has been verified thus far. We report here the clinical characteristics and therapeutic procedure for four patients with mild or severe 2019-nCoV pneumonia admitted to Shanghai Public Health Clinical Center. All the patients were given antiviral treatment including lopinavir/ritonavir (Kaletra (R)), arbidol, and Shufeng Jiedu Capsule (SFJDC, a traditional Chinese medicine) and other necessary support care. After treatment, three patients gained significant improvement in pneumonia associated symptoms, two of whom were confirmed 2019-nCoV negative and discharged, and one of whom was virus negative at the first test. The remaining patient with severe pneumonia had shown signs of improvement by the cutoff date for data collection. Results obtained in the current study may provide clues for treatment of 2019-nCoV pneumonia. The efficacy of antiviral treatment including lopinavir/ritonavir, arbidol, and SFJDC warrants further verification in future study.	[Wang, Zhenwei] Shanghai Univ Tradit Chinese Med, Yueyang Hosp Integrated Tradit Chinese & Western, Dept Resp Dis, Shanghai, Peoples R China; [Chen, Xiaorong; Lu, Yunfei] Shanghai Publ Hlth Clin Ctr, Dept Tradit Chinese Med, Shanghai, Peoples R China; [Chen, Feifei; Zhang, Wei] Shanghai Univ Tradit Chinese Med, Shuguang Hosp, Dept Resp Dis, Shanghai, Peoples R China	Zhang, W (reprint author), Shanghai Univ Tradit Chinese Med, Shuguang Hosp, Dept Resp Dis, Shanghai, Peoples R China.	zhangw1190@sina.com			Shanghai Municipal Key Clinical Specialty [shslczdzk05101]; Shanghai Key Clinical Laboratory of Internal Medicine of Traditional Chinese Medicine [14DZ2273200]	The study is supported by Shanghai Municipal Key Clinical Specialty (shslczdzk05101) and Shanghai Key Clinical Laboratory of Internal Medicine of Traditional Chinese Medicine (14DZ2273200).	[Anonymous], NOT 2019 NCOV INF; Chu CM, 2004, THORAX, V59, P252, DOI 10.1136/thorax.2003.012658; Gralinski LE, 2020, VIRUSES-BASEL, V12, DOI 10.3390/v12020135; Huang CL, 2020, LANCET, V395, P497, DOI 10.1016/S0140-6736(20)30183-5; Lu HZ, 2020, BIOSCI TRENDS, V14, P69, DOI 10.5582/bst.2020.01020; Su B, 2019, FRONT PHARMACOL, V10, DOI 10.3389/fphar.2019.01472; Wang YT, 2017, BIOMED PHARMACOTHER, V91, P393, DOI 10.1016/j.biopha.2017.04.091; Zhu N, 2020, NEW ENGL J MED, V382, P727, DOI 10.1056/NEJMoa2001017	8	1	1	37	37	IRCA-BSSA	TOKYO	PEARL CITY KOISHIKAWA 603, 2-4-5 KASUGA, BUNKYO-KU, TOKYO, 112-0003, JAPAN	1881-7815	1881-7823		BIOSCI TRENDS	BioSci. Trends	FEB	2020	14	1					64	68		10.5582/bst.2020.01030			5	Biology	Life Sciences & Biomedicine - Other Topics	KU2OR	WOS:000519548300010	32037389	Bronze			2020-04-01