Course detail

LET5816 - Pathology and Microbial Control of Arthropods

Credit hours

In-class work
per week
per week
15 weeks
180 hours

Italo Delalibera Junior

Provide theoretical and practical knowledge of the principles of pathology in order to enable the student to recognize the main diseases associated with arthropods specially, insects, mites and ticks. Present
basic and applied knowledge of the main groups of entomopathogens, the importance of these for pest
control and in mass rearing of insects and mites. Presenting a multi-disciplinary approach to the
different non-pathogenic interactions between microorganisms and arthropods of the evolutionary point
of view. Understand the key aspects of the biology, specificity and epizootiology of the most important
microbial control agents. Study the control approaches used with entomopathogens and the possible
risks of these forms of pest management. To learn about methods of production, formulation types and
application techniques microbial products in the field.

Theoretical lessons: History of the pathology and microbial control of arthropods. Diseases of arthropod. Noninfectious diseases, symptoms and diagnosis. Pathophysiology. Immunity and insect defense mechanisms to entomopathogens. Koch's postulates. Viruses, bacteria, protozoa, fungi and entomopathogenic nematodes. Diseases of useful insects. Epizootiology. Types of associations and ecology of the non-pathogenic interactions of microorganism with arthropods. Microorganisms in the digestive tract and extracellular symbionts. Endophytic entomopathogens. Theories of the evolution of virulence. Interactions between micro-organism and micro-organisms: viroses of fungi. Microbial control approaches: classical biological control, augmentative and conservation. Use of viruses, bacteria, fungi, nematodes and microsporidia in pest control. Insect resistance to microbial control agents. Production, formulation, and application techniques of microbial products. Compatibility and synergism of microbial products with chemical pesticides. regulatory and ethical aspects. Risk analysis of microbial control agents.
Practical lessons: Techniques used in pathology of insects and mites, demonstration of detection methods, staining and viability of pathogens. Safety in pathology laboratory. Sterilization. Physiology of infected insects: changes in heartbeat of infected insects, pH of the hemolymph and gut. Materials and culture media, isolation, purification and quantification of entomopathogenic inoculum. Koch's postulates; staining, examination and determination of the main entomopathogenic groups. Modeling the dynamics of pathogens and insect. Microscopic observation and isolation of microorganisms of the digestive tract and extracellular symbionts of insects. Preparation and implementation of microbial control projects by students. Virus purification. Basic concepts on production and bioassays with the following microbial control agents: Bacillus thuringiensis, Bacillus sphaericus (bacteria), Metarhizium anisopliae, Beauveria bassiana, fumosorosea Isaria, Lecanicillium spp (fungi), NPV, GV (virus), nematodes or others. Bioassay on compatibility / synergism of pesticides with entomopathogenic fungi.

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