Course detail

LET5821 - Biotechnology Applied to Entomology

Credit hours

In-class work
per week
per week
15 weeks
60 hours

Fernando Luis Consoli

The objective of this discipline is to introduce students to techniques and to the technological advances in the study and biotechnological exploitation of insects and their associations, involving different areas of knowledge that are important to the development of the field of Agricultural Entomologym devoted particularly to the use of contemporary strategies that would lead to the use sustainable strategies for pest management. Thus, the course aims to proportionate students a muldisciplinary view of research and teaching to allow inter and multidisciplinary approaches in project development.

A short review on cell structure and functioning; DNA amplification and DNA/RNA sequencing approaches; Molecule isolation - electrophoresis; Molecule isolation - chromatography; Molecular and chemical approaches to study insect diversity; Molecular approches for population genetics; Differention gene expression approaches; Exploiting insect genomics; Exploiting insect transcriptomics; Exploiting insect metabolomics; Exploiting insect proteomes; Exploiting insect interactions; Insect control - RNA interference; Insect control - transgenesis; Insect control - paratransgenesis; Insect control - gene editing, CRISPR; Principles and applications of nanobiotecnology.

ABD-ELSALAM, K. & R. PRASAD. 2018. Nanobiotechnology applications in plant protection. Springer. 394p.
AHUJA, S. 2002. Chromatography and separation science. Academic Press, London. 250p.
AVISE, J.C. 2004. Molecular markers, natural history, and evolution. Sinauer Associates, Inc. Publishers, Sunderland, Massachusetts.
BAKER, A.J. 2000. Molecular methods in ecology. Blackwell Publishing Professional. 352p.
BENEDICT, M.Q. 2014. Transgenic insects: techniques and applications. CABI Biotechnological Series. CABI, 388p.
BERTHOLF, R. & R.E. WINECKER. 2007. Chromatographic methods in clinical chemistry and toxicology. John Wiley & Sons, New York. 308p.
BOWTELL, D. & J. SAMBROOK. 2002. DNA Microarrays: a molecular cloning manual. Cold Spring Harbor Laboratory, New York, 712 p.
BRUFORD, M.W., G. HEWITT, A.R. HOETZEL. 2007. Molecular ecology: genes, organisms and processes. Blackwell Publishers. 256p.
CAMPBELL, A.M. & L.J. HEYER. 2006. Discovering genomics, proteomics and bioinformatics. Benjamin Cummings. 340p.
CARAZZI, N. & M. KOZIEL. 1997. Advances in insect control: the role of transgenic plants. CRC Press. 304p.
CARDÉ, R.T. & J.G. MILLAR. 2004. Advances in Insect Chemical Ecology. Cmabridge University Press. 352p.
CONN, P.M. 2003. Handbook of proteomic methods. Humana Press, 524p.
DRAKE, V.A. & A.G. GATEHOUSE. 1995. Insect migration: tracking resources through space and time. Cambridge University Press. 496p.
FREELAND, J.R. 2006. Molecular ecology. John Wiley & Sons, New York. 400p.
HANDLER, A.M. & A.A. JAMES. 2000. Insect trangenesis: Methods and applications. CRC Press, New York. 424p.
HAYES, A.W. 2001. Principles and methods in toxicology. CRC Press, New York. 1887p.
HILLIS, D.M., C. MORITIZ, B.K. MABLE. 1996. Molecular systematics. Sinauer Associates, 2nd. Edition. 655p.
HOFFMANN, K.H. 2014. Insect molecular biology and ecology. CRC Press, Boca Raton. 411p.
MURPHY, D.B. 2001. Fundamentals of light microscopy and electronic imaging. Wiley-Liss. 360p.
HOFFMAN, E. DE & V. STROOBANT. 2003. Mass spectrometry: Principles and applications. John Wiley & Sons. 407p.
HOKKANEN, H.M.T. & A.E. HAJEK. 2004. Environmental impacts of microbial insecticides: need and methods for risk assessment (Progress in Biological Control, >1). Kluwer Academic Publishers. 272p.
HOY, M.A. 2001. Insect molecular genetics: an introduction to principles and applications. Academic Press, New York. 2nd Edition. 600p.
KINTER, M. & N.E. SHERMAN. 2000. Protein sequencing and identification using tandem mass spectrometry. Wiley-Interscience Series. 301p.
KJELLSSON, G., V. SIMONSEN & K. AMMANN. 1997. Methods for risk assessment of transgenic plants: pollination, gene-transfer and population impacts. Birkhauser Boston. 308p.
KOUL, O. & G.S. DHALIWAL. 2004. Transgenic crop protection: concepts and strategies. Science Publishers. 420p.
MALIK, V.P. & E.P. LILLEHOJ. 1994. Antibody techniques. Academic Press. 353p.
MILLAR, J.G. & K.F. HAYNES. 1998. Methods in chemical ecology. Kluwer Academic Publishers. 375p.
MOUNT, D. 2004. Bioinformatics: sequence and genome analysis. 2nd Edition. Cold Spring Harbor Laboratory. 665 p.
NEI, M. & S. KUMER. 2001. Molecular evolution and phylogenetics. Oxford University Press. 333p.
PERIASAMY, A. 2001. Methods in cellular imaging. American Physiological Society. 434p.
RAMAN, C., M.R. GOLDSMITH et al. 2015. Short views on Insect Genomics and Proteomics, vols. 1 & 2. Book Series Entomology in Focus, Springer.
ROTHE, G.M. 1994. Electrophoresis of enzymes: laboratory methods. Spring-Verlag. 307p.
SAMBROOK, J. & D. RUSSELL. 2001. Molecular Cloning: A laboratory Manual. 3rd Edition. Cold Spring Harbor Laboratory, New York, 2344 p.
SCHALKHAMMER, T.G.M. 2007. Analytical biotechnology. Birkhauser. 200p.
SIMPSON, R.J. 2002. Proteins and proteomics: a laboratory manual. Cold Spring Harbor Laboratory Press, 900p.
THACKER, J.R.M. 2002. An introduction to arthropod pest control. Cambridge University Press. 360p.
VEENSTRA, T.D. & J.R. YATES. 2006. Proteomics for biological diversity. Wiley-Liss, London. 326p.
VILCINSKAS, A. 2011. Insect Biotechnology. Vol. 2. Springer, Dordrecht. 268p.
VILCINSKAS, A. 2013. Yellow Biotechnology. Vol. 2. In Advances in Biochemical Engineering/Biotechnology 136. Springer-Verlag, Berlin. 209p.
WESTERMEIER, R. 2001. Electrophoresis in Practice. 3rd Edition. Wiley-VCH, London. 368p.
WILSON, K. & J. WALKER. 2005. Principles and techniques of biochemistry and molecular biology. Cambridge University Press, Cambridge.
WYATT, T.D. 2003. Pheromones and animal behaviour: communication by smell and taste. Cambridge University Press. 408p.
XIONG, J. 2006. Essential bioinformatics. Cambridge University Press, Cambridge. 352p.