LGN5835 - Bioinformática Aplicada

Carga Horária

Docentes responsáveis
Gabriel Rodrigues Alves Margarido

Objetivo
A disciplina oferecerá aos alunos uma visão teórico-prática das tecnologias disponíveis para o
sequenciamento genômico em larga escala, com ênfase nas ferramentas disponíveis para análise dos
dados. Capacitar os alunos a aplicar estratégias de genotipagem e transcriptômica ao melhoramento
genético.

Conteúdo
Genômica e Bioinformática. Tecnologias modernas para o sequenciamento de DNA. Pré-processamento
de dados de sequenciamento de nucleotídeos. Alinhamento de sequências biológicas. Sequenciamento e
montagem de novo de genomas completos. Descoberta de polimorfismos e genotipagem:
resequenciamento genômico; sequenciamento de bibliotecas de representação reduzida (GBS e RAD).
Genômica funcional e transcriptômica. Montagem de novo de transcriptomas. Expressão gênica
diferencial. Estudos de enriquecimento funcional. Entre os programas e plataformas utilizados,
destacam-se: Bowtie, HISAT, BWA-MEM, IGV, TASSEL-GBS, GATK, FreeBayes, Trinity, R,
R/Bioconductor, edgeR, goseq.

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