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Horticulture Biotechnology Lab

 

The research projects carried out at the Horticulture Biotechnology Lab are related to the development of biotechnological tools for in vitro Citrus breeding. The production of Citrus somatic hybrids by protoplast chemical fusion has been conducted in cooperation with the Plant Biotechnology Lab at CENA (Dr. Beatriz Madalena Januzzi Mendes) since 1996. Investigations on genetic transformation of Citrus rootstocks and scions, started in 1999, were conducted for the optimization of protocols. Currently, the introduction of horticultural important genes and initial transgenic plant evaluation have been carried out.

 

 

 

 

..::INFRASTRUCTURE

 

The Lab is structured for research on plant tissue and cell culture, including rooms and equipments for culture media preparation, aseptic manipulation and incubation. Equipments for molecular analysis, and greenhouses for acclimatization of plants originated from in vitro manipulations are also available.

 


 

 

..::Products and Processes

 

Fifteen Citrus somatic hybrids with potential to serve as rootstocks and/or scions have been developed so far. These hybrids are presently undergoing field evaluations. Research related to protoplast fusion continues to be developed with the goal of obtaining new somatic hybrids combinations.

Regarding transgenic technology, more than 200 transgenic Citrus lines have been developed, harboring genes with potential for pathogen resistance.

 

 

Financial Support

• CNPq
• FAPESP
• Fundecitrus

Contactl: Prof Dr. Francisco de Assis Mourão Filho