Credit hours
In-class work per week |
Practice per week |
Credits |
Duration |
Total |
2 |
2 |
8 |
15 weeks |
120 hours |
Instructor
Mateus Mondin
Objective
Genome organizations in higher organisms with emphasis to plants, however, with a solid link to human and other animal genome organizations. Distribution, organization, and evolution of repetitive DNAs as satellites, transposable elements, multigenic families, etc. Contextualization of the gene inside the complex genomes. Relationship between genome size and repetitive DNA. Polyploidy and the complexity of the genome organization. Molecular organization of the chromatin, with particular emphasis on nucleosomal organization and DNA packing. Definition of chromosomal packing levels and their relationship to gene expression. Chromosomal territories and their role in the control of gene expression. Epigenetics and its role in the genome organization and the transcription and replication of the DNA. Histone structures and their modifications. DNA methylation and its role in genome organization and the control of gene expression. Epigenetic inheritance and memory. Epigenetics role in the GxE.
Content
Eukaryote Genome Organization
Concepts of Genome Size and the C-value paradox
Repetitive DNA sequences and their distribution into genome - dispersed and in tandem
Classes and families of Repetitive DNAs - transposable elements and Satellite DNAs
Functional Structures of the Genome - chromomeres, heterochromatin, nucleolus organizer region, telomeres and centromeres
Up and Down of genome size
DNA package
Histones, DNA, Nucleosome and Chromatin
Supra-molecular Structure of the Chromatin - genome organization inside the nucleus and chromosome territories
Chromatin dynamics and its relationship to gene expression
Epigenetic Modifications and the control of Chromatin Dynamics - histones and DNA modifications
How abiotic and biotic stress can affect the epigenetic indexing and its consequences to gene expression
Complex Genome Functioning - polyploidy, genomic dominance and imprinting
Epigenetic inheritance applied to Plant Breeding, Biotechnology and Evolution07
Bibliography
Turner, Bryan M. Chromatin and gene regulation: molecular mechanisms in epigenetics. John Wiley & Sons, 2008.
Wolffe, Alan, ed. Chromatin: structure and function. Vol. 12. No. 1. Academic press, 1998.
Sumner, Adrian T. Chromosomes: organization and function. John Wiley & Sons, 2008.
Ferguson-Smith, Anne C., John M. Greally, and Robert A. Martienssen, eds. Epigenomics. Springer Science & Business Media, 2008.
ALBERTS, B.; JOHNSON, A.; LEWIS, J.; RALF, M.; ROBERTS, K.; WALTER, P. Molecular biology of the cell. 5ª ed. New York: Garland Science, 2008. 1548p.
Brown, Terence A. Genomes. Garland science, 2006.
Ugarkovic, Durdica. Centromere: structure and evolution. Vol. 48. Springer Science & Business Media, 2009.
KIPLING, D. The telomere. Oxford; Oxford University Press, 1995.
Spillane, Charles, and Peter C. McKeown. "Plant Epigenetics and Epigenomics." 2014
Grafi, Gideon, and Nir Ohad. Epigenetic memory and control in plants. Springer, 2013.