Course detail

LCF5899 - Biotechnology Techniques in the Production of Species Plantlets Forestry

Credit hours

In-class work
per week
per week
15 weeks
120 hours

Julieta Andrea Silva de Almeida
Paulo Hercílio Viegas Rodrigues

The course aims to achieve the following objectives:
1. To provide an understanding of the productive processes in a Biofactory of forest species;
2. To develop mechanisms related to automation in in vitro propagation processes and
acclimatization of forest species;
3. Develop competence and critical capacity regarding the study and domestication of new
(native) forest species.

I. Introduction
A. Presentation of the program's objectives, work methodology and tasks.
B. General aspects of a Biofactory.
II. Introduction to Plant Tissue Culture
A. Tissue culture in the production of seedlings
1. Culture media
2. Growth regulators
3. Pathogen-free clones
B. Structure of a Biofactory
1. Architecture
2. Equipment
3. Production flowchart
III. Germplasm bank and in vitro establishment
A. Collection and maintenance of a germplasm bank (BG)
1. Substrate
2. Water quality
3. Phytosanitary control
4. Use of protected cultivation to maintain a BG
B. In vitro establishment
1. Selection of explants
2. Culture media
3. Effect of pH
4. Effect of the gelling agent
5. Oxidation
IV. Use of bioreactors
A. Types of bioreactors
B. Automation of the time immersion system
C. Chemical sterilization
D. Autotrophic photo system
V. Use of different light spectra from LED
A. Photo morphogenesis
B. Slow grown “in vitro” storage
C. Environmentally Modified Organisms (OAM)
V. Seedling quality
A. genetic variation
B. epigenetic variation
VII. Embryogenesis in in vitro cultivation of forest species
A. History of the research in somatic embryogenesis
B. Physiological and biochemical aspects of somatic embryogenesis
C. Indirect and direct somatic embryogenesis in plants
D. Factors affecting the control of the occurrence of somatic embryogenesis
E. Comparison between zygotic embryogenesis and somatic embryogenesis
VIII. Acclimatization techniques
A. Suitable substrate
B. Greenhouses
C. Containers
D. Humidity control
E. Temperature control
F. Spectra of light and light intensity
G. Automation systems for acclimatization
IX. Cost evaluation
A. Cost management
B. Traceability
X. Native species
A. Economic potential vs. market
B. Domestication strategies
C. Legislation on the use of native plants
D. Registration of new species at MAPA

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Periódicos da Área:
Tree Physiology
Revista Árvore
Revista Scientia Forestalis
Plant Physiology
Plant Cell, Tissue and Organ Culture