Credit hours
In-class work per week |
Practice per week |
Credits |
Duration |
Total |
3 |
1 |
8 |
15 weeks |
120 hours |
Instructor
Ivan Paulo Bedendo
Objective
Provide the student knowledge and mastery of techniques most commonly used in experimentation in
Plant Pathology.
Content
Techniques for sterilization of instruments and materials used in the laboratory, preparation and use of
culture media, isolation of pathogens, production and quantification of inoculum, inoculation methods of
pathogens and disease assessment, maintenance of fungal and bacterial cultures, understanding of
statistical designs, use of serological and molecular techniques for disease diagnosis.
Bibliography
Amorim, L.; Rezende, JAM; Bergamin Filho, A. Manual de Fitopatologia : Princípios e Conceitos Editora
Agronômica Ceres, Ouro Fino, 2018. Alfenas, A.C.; Gonçalves, R.C.; Mafia, R.G. Métodos em
Fitopatologia. Editora UFV, Viçosa 2007. 329 p. Dingra, O.D.; Sinclair, J.B. Basic Plant Pathology
Methods. CRC Press, Boca Raton, 1985. 355 p. Eiras, M.; Galleti, S.R. Técnicas de Diagnóstico de
Fitopatógenos. Devir livraria. São Paulo. 2012.190 p. Kiraly,Z.; Klement, A.; Solimosy, F.; Voros, J.
Methods in Plant Pathology. Academiai Kiado, Budapest. 1970, 509 p. Kirsop, B.E.; Doyle, A. Maintenace
of microorganisms and cultured cells. Academic Press, New York. 1991. 308 p. Menezes, M.; Silva-
Hanlin, D. Guia prático de fungos fitopatogênicos. Editora UFRPE, Recife. 1997. 106 p. Romeiro, R.S.
Métodos em bacteriologia de plantas. Editora UFV, Viçosa. 2001 279 p. Schaad, N.W.; Jones, J.B.; Chun,
W. Laboratory Guide for Identification of Plant Pathogenic Bacteria. American Phytopathological Society.
St. Paul. 2001. 373 p