Credit hours
In-class work per week |
Practice per week |
Credits |
Duration |
Total |
2 |
2 |
8 |
15 weeks |
120 hours |
Instructor
Alberto Soares Correa
Objective
The course aims to address main issues on arthropods molecular ecology by theoretical and practical
ways. Speciation, population genetics, intra- and interspecific interactions and adaptation will be studied
in an evolutionary context. We will show the concepts and techniques applied in molecular ecology for
the development of sustainable management strategies of pests and beneficial arthropods of
agricultural, medical and veterinary importance
Content
(1) Structure and function of DNA and RNA of eukaryotic, prokaryotes and organelles; (2) Replication,
transcription and translation; (3) NCBI (gene sequences, primers and BLAST); (4) DNA and RNA
extraction and primers design; (5) polymerase chain reaction (PCR); (6) PCR molecular markers
(dominates and co-dominates); (7) concepts and processes of speciation; (8) Introduction to molecular
evolution and phylogeny; (9) Molecular identification of species, DNA barcoding; (10) Genetic diversity,
indices and data analysis; (11) populational structure and gene flow; (12) Demography and
Phylogeography; (13) Genomic and Adaptation (14) and (15) Case studies applied in Integrated Pest
Management.
Bibliography
Freeland, J. R., Kirk, H. and Petersen, S. (2020) Molecular Ecology, Third Edition, John Wiley & Sons,
Ltd, Chichester, UK.
Hahn M. W. (2018) Molecular Population Genetics, First Edition, Sunderland, MA: Sinauer Associates.
Poelchau, M. F., … & Shoemaker, D. (2016). Agricultural applications of insect ecological genomics.
Current opinion in insect science, 13, 61-69.
Hoy, M.A. (2013) Insect Molecular Genetics: an Introduction to Principles and Applications, Third Edition,
Elsevier’s Science & Technology Rights Department in Oxford, UK.
Gilbert, L. I. (2012) Insect Molecular Biology and Biochemistry, First Edition, Elsevier’s Science &
Technology Rights Department in Oxford, UK.
Hartl, D. I. & Clark, A. G. (2010) Princípios da Genética de População, 4ª Edição, Editora: Artmed, Porto
Alegre, RS, Brasil.
Beebee, T. J. C. (2008) An introduction to molecular ecology. Oxford University Press.
Ridley, M. (2006) Evolução, 3ª Edição, Editora: Artmed, Porto Alegre, RS, Brasil.
Avise, J. C. (2004) Molecular Markers, Natural History, and Evolution, Second Edition, Harvard University
Press, Cambridge, MA, US.
Lewin, B. (2004) Genes VIII. 8th Edition, Upper Saddle River: Pearson Prentice Hall.
Carlton, J. (2003) Invasive species: vectors and management strategies. Island Press.
Ehler, L. E., Sforza, R., Mateille, T. (2003) Genetics, Evolution and Biological Control, First edition, CABI
Publishing.
Avise, J. C. (2000) Phylogeography: The History and Formation of Species, Harvard University Press,
Cambridge, MA, US.